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이희정 ( Hee Jung Lee ),정헌상 ( Heon Sang Jeong ),박춘근 ( Chun Geon Park ),이정훈 ( Jeong Hoon Lee ),박충범 ( Chung Berm Park ),김종태 ( Chong Tai Kim ),최애진 ( Ae Jin Choi ) 한국산업식품공학회 2014 산업 식품공학 Vol.18 No.4
본 연구는 생약재로 이용되는 참당귀의 다용도 기능성 소재 개발을 통한 이용가치를 높이기 위하여 효소처리를 활용한 기능성 다당체 분리방법에 대한 연구를 수행하였다. 참당귀의 세포벽 성분 분해를 위한 최적의 효소는 Viscozyme L 로 선정되었다. 다당체 분리를 위한 효소처리는 단백분해 효소(Alcalase 및 Flavorzyme)와 전분분해효소(Termamyl 120L)를 Viscozyme L과 함께 복합적으로 처리한 VAFT 처리구가 추출 수율과 총당 함량이 각각 12.20%, 76.80% 로 다른 효소처리구(T, AFT 처리구)에 비하여 가장 높았으며, 제거된 전분 함량 역시 29.62%로 가장 높게 나타났다. 또한 비전분다당체 함량이 22.58%로 T 처리구의 20.78%과 AFT 처리구의 21.98%에 비하여 유의적(p<0.001) 으로 가장 높게 나타나 참당귀 다당체 분리를 위한 최적 효소처리 조건으로 선정되었다. 비전분다당체의 주성분인 arabinose, galactose의 함량은 대조구보다 T, AFT, VAFT의 모든 효소처리구에서 유의적(p<0.001)으로 높게 나타났다. 또한 참당귀 다당체의 분자량 분석을 통하여 대조구 (491,000 Da)에 비하여 VAFT 효소처리구가 13,000 Da로 가장 저분자화되는 것을 확인할 수 있었다. 따라서 VAFT 효소처리에 의한 참당귀 기능성 다당류의 효율적인 분리방법은 참당귀를 활용한 새로운 산업적 기능소재 및 제품 개발에 활용 가능성이 높을 것으로 기대되었다. This study conducted enzyme treatments to maximize the extraction of polysaccharide components in Angelica gigas Nakai (A. gigas). An extraction method of polysaccharides was established by using different enzyme treatments for the degradation of plant cell walls. Total carbohydrates and removed starch of A. gigas increased after enzyme treatments such as Viscozyme (V), Alcalase (A), Flavorzyme (F) and Termamyl 120L (T). The yield and total carbohydrates of VAFT were 12.20% and 76.80%, respectively; an increase of 1.4 times that of T and AFT and showed a significant difference (p<0.05). Removed starch content also increased by more than three times the control in water-extractable polysaccharides. Non-starch polysaccharide content of VAFT was 22.58%; it increased about 1.7 times that of the control (12.84%) and it was the highest compared to T and AFT. Arabinose and galactose, which were the main components of A. gigas polysaccharides, increased from 1.5 to 2.2 times the control in the enzyme treatment groups. The high molecular weight fraction I of the control, depending on enzyme treatment conditions, was about 491,282 Da. It degraded into a low molecular weight fraction ranged from 282,459 to 16,362 Da. The molecular weight of polysaccharides treated by the VAFT decreased to an average of 13,000 Da. So, VAFT was selected as the optimizing condition for extracting water extractable polysaccharides from A. gigas. As a result of this study, it is expected that A. gigas will be widely used for industrial materials and useful to develop and research materials of different medicinal plants which are not yet known.
16 MDCT장비에서 Dose Modulation Program인 CareDose와 CareDose4D의 CTDIvol값 비교
이희정(Hee Jeong Lee),권성옥(Seong Ohk Kwon),김주(Joo Kim),서동수(Dong Soo Suh),정성민(Seong Min Chung),임청환(Cheong Hwan Lim) 대한전산화단층기술학회 2005 대한CT영상기술학회지 Vol.7 No.1
Purpose The aim of this study is to apply to clinic a proper technical factor by comparing the value of CTDIvol with dose modulation program of CareDose and CareDose4D in 16 MDCT. Material & Method The value of CTDIvol of constant and variable mA(140mA) was measured in Siemens somatom sensation 16 VB10B CT. The measurement of CTDIvol of CareDose and CareDose4D was performed with technical factor variation. With ART phantom of human shape, CareDose used the Siemens somatom sensation 16 VA70C, and CareDose4D done Siemens somatom sensation 16 VB10B. Result The value of CTDIvol of constant mA and variable mA of CareDose4D was 9.8mGy to 10.92mGy, and 4.13mGy to 5.58mGy, respectively. The value of CTDIvol by kVp variation was minimum 2.53mGy, 2.335mGy at 80kVp and maximum 11.34mGy to 10.18mGy at 140 kVp in the two mode. The value of CTDIvol by table pitch variation in CareDose didn’t vary with 8.14mGy, that by pitch variation of 1, 1.2, 1.5, 2 was 7.38, 7.36, 5.94, 4.60mGy, respectively. The value of CTDIvol by slice thickness variation in CareDose also didn’t vary with 8.14mGy, but CareDose4D increased from 7.26 to 7.35mGy according to increment of thickness. When the tube rotation time was 0.5, 0.75, 1, 1.5 second, the value of CTDivol was 8.14, 8.56, 8.14, 8.21mGy in CareDose and 7.27, 7.30, 7.29, 7.38mGy in CareDose4D, respectively. The value of CTDIvol by scan type was 17.16 and 17.97mGy in CareDose and CareDose4D with sequence mode, and 22.06 and 22.65mGy with spiral mode. Conlclusion The value of CTDivol was decreased by appling CT with dose modulation program for reduction of exposed dose. The value of CTDIvol excluding scan type of technical factor was decreased more in CareDose4D than in CareDose.