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3T3-L1 지방전구세포에 대한 다양한 주파수 진동의 지방 생성 억제 효과
이영훈,이석호,정혜빈,정용찬,김민환,이은미,김지현,Lee, Yeong Hun,Lee, Seok-Ho,Jung, Haebin,Jung, Yong Chan,Kim, Min Hwan,Lee, Eun Mi,Kim, Chi Hyun 대한의용생체공학회 2021 의공학회지 Vol.42 No.1
Vibration is a mechanical cue that can be applied to adipose tissues for the purpose of treating obesity. However, the exact correlation between vibration and other anti-adipogenic pathways, such as development of cytoskeleton and apoptosis, remains unknown. The objective of this study was to investigate the unknown anti-adipogenic effects of vibration with varied frequencies on preadipocytes. 3T3-L1 preadipocytes were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 5% calf serum at 37 ℃ with 5% CO2 in a humidified incubator. Vibration was generated using Arduino Uno microcontroller and vibration motor module with 1 V DC, and applied to preadipocytes for 3 days. Frequency conditions were set to 20, 55, and 90 Hz. Then, the expressions of p38 pathway, ROCK-1, α-actinin, Bax, Bcl-2, caspase-9, 8, and 3 were analyzed with western blot. As a result, p38 pathway was inhibited in 55 and 90 Hz while ROCK-1 and α-actinin were expressed in 20 Hz. Caspase-3, a terminal apoptotic factor, was activated in 20 Hz via extrinsic pathway rather than intrinsic pathway. Results suggest that various frequencies of vibration can inhibit adipogenesis via different pathways which sheds light on future mechanotransduction applications of vibration for the treatment of obesity.
이영훈,Lee, Yeong-Hun 한국대학교육협의회 2007 大學敎育 Vol.148 No.-
대학에서 기업이 필요로 하는 역량을 충분히 향상시킬 수 있는 교육을 실시하며 가시적인 성과를 보인다면, 기업도 대학교육을 위해 훨씬 더 적극적인 지원 방안을 모색하게 될 것이다. 그러기 위해서는 기업과 대학이 더 자주 만나야 한다. 신입사원들에게 요구하는 대인관계와 협동정신을 대학과 기업이 서로를 향해 발휘한다면, 대학과 기업, 그리고 대학을 졸업한 신입사원 모두가 Win-Win하는 결과를 가져올 수 있을 것이다
이영훈,Lee, Yeong-Hun 전국농업기술자협회 2012 농업기술회보 Vol.49 No.4
콩에서 가장 문제가 되는 병해로는 콩모자이크바이러스병, 잎이 조기에 떨어지는 불마름병과 들불병, 종자의 품위에 영향을 주는 자주무늬병, 미이라병 등이 있다. 이들 병해는 고온 다습한 재배환경에서 많이 발생하고 병의 확산 속도가 빨라져 큰 피해를 주게 된다.
이영훈 ( Lee Yeong Hun ) 한국고문서학회 2003 古文書硏究 Vol.23 No.-
In 1852, the governor of Chungju-mok ordered to survey the Homyungs(戶名) and their real persons. Homyung indicated the assumed name of the taxpayer. According to his order, The Survey Book on Juhos of each village was reported. Juho meant the real person of Homyung, namely the real taxpayer. On those periods, the land tax was collected from an imaginary person whom the Homyung supposed to be. For that reason, there occured so much problems which finally made the governor of Chungju-mok to survey the Homyungs and compile The Survey Book on Juhos of each village. In origin, the Juho indicated the main part of the whole households and the vocabulary itself as a legal one was used only in the field of census registration. But The Sumey Book on Juhos, a historic documents discovered recently, tells us that the legal vocabulary Juho was used not only in the census registration but also in the survey of taxpayers, It supplies an important viewpoint in pursuing the real and whole meanings of Juho from now on.