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최재석,유대석,최연희,연규환,홍경식,이병회,김혜진,김현기,김은주,노성환,정영철,김영섭,유시용,Choi, Jae-Seok,Yoo, Dae-Seok,Choi, Yeon-Hee,Yon, Gyu-Hwan,Hong, Kyung-Sik,Lee, Byung-Hoe,Kim, Hye-Jin,Kim, Hyun-Ki,Kim, Eun-Ju,Roh, Seong-Hwan,Jeong 한국생약학회 2007 생약학회지 Vol.38 No.2
We investigated the contents of crude saponin in the decoctions of Platycodi Radix prepared by the different extraction processes. Each lyophilized decoctions prepared by the different protocol were suspended in water and partitioned with ethylacetate (EtOAc) and n-butanol (BuOH), which gave the EtOAc fraction, BuOH fraction and the remaining water fraction, respectively. The content of crude saponin, which was estimated as the amount of BuOH fraction, and the HPLC profiles of the BuOH fraction were analyzed, and compared each others. The crude saponin content in the decoctions were increased proportionally to the increment of the extraction time, amount of water, extraction temperature and the number of repetition for extraction. Consequently, the optimized conditions were deduced to be suitable and recommendable for the preparation of Platycodi Radix ; the water amount needed for the extraction be 15-20 volumes to crude material, temperature for extraction be optimal at $85{\sim}100^{\circ}C$, extraction time be more than 5 hours and the repetition of extraction was 2 times.