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Cytodex 3 미립담체를 이용한 간세포의 구상체 배양에서 간세포와 미립담체 간의 최적 비율
신우영(Woo Young Shin),이건욱(Kuhn Uk Lee),이해원(Hae Won Lee),조응호(Eung-Ho Cho),이남준(Nam-Joon Yi),서경석(Kyung-Suk Suh) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.3
Purpose: The mass cultivation of functional hepatocytes is a key factor of a bioartificial liver. Combining spheroid and microcarrier cultures has been applied for enhancing the cell viability and metabolic activities. Hence, the optimal number of hepatocytes per microcarrier was investigated. Methods: Firstly, spheroid cultures were carried out with 1 g Cytodex 3 microcarrier plus 2×10?, 4×10? and ?×10? viable hepatocytes per flask. The numbers of hepatocytes per microcarrier were approximately 666.7, 133.3 and 26.7, respectively. The control group consisted of a spheroid culture of 4×10? hepatocytes without any microcarrier. According to the primary experimental results, spheroid cultures with 1×10? of hepatocytes plus 1 g, 2 g and 3 g of the Cytodex 3 microcarrier were performed. The numbers of hepatocytes per microcarrier were approximately 33.3, 16.7 and 11.1, respectively. The control group consisted of a spheroid culture of 1×10? hepatocytes. The cell viabilities were assayed using a Cell Counting Kit-8; with the albumin production assayed using ELISA. Results: According to the primary experiment, the group consisting of 26.7 hepatocytes per microcarrier showed the highest viability (P<0.01). However, there was no statistical difference in the albumin production between the groups (P=0.744). The second Experiment showed the groups consisting of 11.1 and 16.7 hepatocytes per microcarrier had higher viabilities than the other hepatocyte and control groups (P<0.01). The albumin production was similar for each group (P=0.187). Conclusion: With respect to their application to a bioartificial liver, about 130 hepatocytes per microcarrier was appeared to be good for the mass cultivation of a hepatocytes spheroid culture using the Cytodex 3 microcarrier.
연구논문 : 수술 전 알파태아단백치가 높은 간세포암 환자에서 간절제술 후 알파태아단백만을 이용한 추적관찰이 가능한가?
신우영 ( Woo Young Shin ),서경석 ( Kyung Suk Suh ),김태훈 ( Taehoon Kim ),전영민 ( Young Min Jeon ),이남준 ( Nam Joon Yi ),이건욱 ( Kuhn Uk Lee ) 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.2
Background/Aims: The follow-up strategy after hepatectomy for hepatocellular carcinoma (HCC) usually depends on the experience of physician, resulting in frequent imaging studies, which leads to increased cost. Hence, we investigated the role of monitoring alpha-fetoprotein (AFP) levels after hepatectomy in patients with preoperative high AFP. Methods: From January 2000 to December 2004, 66 patients who underwent curative hepatectomy due to HCC with preoperative AFP level >400 ng/ml were reviewed. Changes in AFP level after the operation were investigated. The recurrence was suspected in case of two consecutive increase of AFP over cut-off value. Cut-off value was determined by ROC curve. All patients were divided into 2 groups: patients who met the definition (Group S) and those who didn`t (Group D). Results: AFP level of 20ng/ml was proposed as the cut-off value for diagnosis of recurrence by ROC curve. Thirty two patients who didn`t have the AFP level decreased below 20 ng/ml after the resection had HCC recurred, whereas 16 out of 34 patients who had AFP decreased had tumor recurrence. The AFP level of patients without recurrence was kept below 20 ng/ml during the follow-up. The AFP level of 44 out of 48 recurred patients increased over 20ng/ml upon recurrence. By definition, group D were 5 patients. In 4 patients of group D, the AFP level didn`t increase above 20 ng/ml upon recurrence. These patients had HCC and they recurred 1 year after the surgery. Conclusions: In patients with preoperative AFP level >400 ng/ml, the AFP level tended to increase above 20ng/ml at recurrence mostly within 1 year. Hence, we proposed that these patients could be monitored by only AFP until 1 year after surgery.
신우영(Woo-young Shin),송정수(Jung Soo Song),김정하(Jung-ha Kim) 대한임상노인의학회 2021 대한임상노인의학회지 Vol.22 No.2
Polypharmacy is a major public health concern globally with aging populations and the increase in the prevalence of multimorbidity. This review provides an overview of polypharmacy and its management programs in older patients and describes the development direction and challenges of polypharmacy management strategies while considering the current situation in Korea. Although polypharmacy is often regarded as the routine use of at least five medications, the definition is more useful in terms of clinical appropriateness rather than the number of medications administered. The goal of polypharmacy management should be to ensure rational prescription based on clinical evidence and appropriate medication use in consideration of the patient’s individual factors. In order for doctors, nurses, and pharmacists to collaborate within a multidisciplinary team to ensure a comprehensive medication review, it would be effective to implement a management model in the form of a systematic program within the healthcare delivery system. Some countries are introducing sustainable polypharmacy management programs and developing standardized polypharmacy guidelines. It is required to conduct further benchmark studies of polypharmacy management programs in other countries that have proven their effectiveness while considering the Korean situation.
70% 간절제술을 받은 쥐에서 피브린젤을 이용한 중간엽줄기세포의 생체내 간세포 분화
신우영(Woo Young Shin),정성은(Sung Eun Jung),민혜숙(Hye Sook Min),이해원(Hae Won Lee),조응호(Eung-Ho Cho),이남준(Nam-Joon Yi),서경석(Kyung-Suk Suh),이건욱(Kuhn Uk Lee) 한국간담췌외과학회 2010 한국간담췌외과학회지 Vol.14 No.2
Purpose: To investigate the differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) into hepatocytes by cell transplantation using fibrin gels in a 70% hepatectomized rat model. Methods: MSCs were isolated from Sprague-Dawley rats. MSCs (1.5×107 cells) were mixed with fibrin gels and injected immediately into the abdominal cavity of 70% hepatectomized rats. Fibrin-gels consisted of 500 IU/ml of thrombin and 90 mg/ml of fibrinogen. Transplanted MSCs in the fibrin scaffold were retrieved from surgically opened peritoneal cavities of rats on days 5, 10, 15, and 21 after the operation. The specimens were analyzed histologically and immunohistochemically. Results: On H&E staining, MSCs from hepatectomized rats had changed to a round shape, while MSCs of the control group kept their spindle shape. When the fibrin matrix was biodegraded at day 15, the morphology of the MSCs had changed to hepatocyte-like cells without sinusoids and the hepatocyte-like cells had formed a three-dimensional tissue permitting cell-to-cell contacts within the matrix. On immunohistochemistry, MSCs expressed the hepatocyte markers cytokeratin 18, albumin, and alpha-fetoprotein, after 15 days of transplantation. Conclusion: When bone marrow-derived MSCs are transplanted using fibrin gels in the 70% hepatectomized rat, MSCs differentiate into hepatocyte-like cells and are conglomerated so that they form three-dimensional tissue-like hepatocytes without sinusoids.