Schwanniomyces castellii CBS 2863(ATCC 26077)으로부터 $\alpha$-Amylase 정제 및 특성

박종천,배석,임선영,이진종,이향,전순배,Park, Jong-Chun,Bai, Suk,Lim, Suhn-Young,Lee, Jin-Jong,Lee, Hyang,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6

The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.

Schwanniomyces castellii CBS 2863으로부터 ${\alpha}$-Amylase 유전자 Cloning

박종천,배석,전순배,Park, Jong-Chun,Bai, Suk,Chun, Bai-CHun 한국미생물학회 1994 미생물학회지 Vol.32 No.1

Schwanniomyces castellii의 제놈 DNA로 제조된 유전자 은행으로부터 cloning된 ${\alpha}$-amylase 유전자가 Sacchromyces cerevisiae에서 발현되었다. Cloning된 삽입 DNA 절편의 크기는 약 5.0 kb이었고, Southern 및 immunoblot 분석 결과 cloning된 ${\alpha}$-amylase 유전자가 Sch. Castellii로부터 유래되었음이 확인되었다. S. cerevisiae SHY3 형질전환체에서 Sch. Castellii ${\alpha}$-amylase 유전자발현은 모균주에 비해 낮았으나, 단백질의 분자량 및 효소의 성질은 Sch. Castellii에서 분리한 ${\alpha}$-amylase의 그것과 차이가 없었다. The gene encoding ${\alpha}$-amylase of Schwanniomyces castellii was cloned in Saccharomyces cerevisiae. The 5.0-kilobase insert was shown to direct the synthesis of ${\alpha}$-amylase. Southern blot analysis confirmed that this ${\alpha}$-amylase gene was derived from the genomic DNA of Sch. castellii. Immunoblot analysis showed that ${\alpha}$-amylase production from S. cerevisiae transformant was less than that of donor strain. The ${\alpha}$-amylase secreted from S. cerevisiae transformant was shown to be indistinguishable from that of Sch. castellii on the basis of molecular weight and enzyme properties.

Schwanniomyces occidentalis var. persoonii CBS 2169 $\alpha$-Amylase 유전자의 Nucleotide Sequence

박종천,배석,오상진,이진종,전순배,Park, Jong-Chun,Bai, Suk,Oh, Sang-Jin,Lee, Jin-Jong,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6

The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.

보문 : Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향

남상영(Sang Young Nam),김경미(Kyoung Mi Kim),박종천(Jong Chun Park),주선종(Seon Jong Joo),정재현(Jai Hyun Jung) 한국식품저장유통학회(구 한국농산물저장유통학회) 1997 한국식품저장유통학회지 Vol.4 No.1

광양만 생태계의 미생물군집에 관한 환경요인 분석

박종천,이우범,주현수 ( Jong Chun Park,Woo Bum Lee,Hyun Soo Joo ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

In Kwangyang bay, the biological and ecological surveys were carried out at seventeen sites both in surface and bottom water from July 1996 to May 1997. Fifteen abiotic and biotic parameters were determined on each sample. These data were subjected to simple regression, multiple stepwise regression and the principal factor analysis-to scrutinize the effect of environmental conditions on water quality and ecosystem. By the results of these analyses in Kwangyang bay, ecosystem were regulated by five factors for four seasons. The results showed that distribution of the microbial population was mainly influenced by inflow of fresh water, nitrogenous nutrients, suspended solids and water temperature. The overall size of microbial population was greater ten and five times than those obtained in 1982 and 1992, respectively.

광양만의 퇴적물에 대한 이화학적 조성 및 중금속 함량

박종천,김진,이우범,이성우,주현수,Park, Jong-Chun,Kim, Jin,Lee, Woo-Bum,Lee, Sung-Woo,Joo, Hyun-Soo 한국하천호수학회 2000 생태와 환경 Vol.33 No.1

본 연구는 광양만 퇴적물의 이화학적 조성과 중금속 함량을 알아보기 위해 함수율, COD, $H_{2}S$, 입도 및 10가지의 중금속을 17개 조사지점에 대해 분석${\cdot}$조사하였다. 조사기간동안 나타난 결과로서 퇴적물의 함수율 변화는 35.5${\sim}$53.8%를 나타내었다. COD와 $H_{2}S$는 각각 3.8${\sim}$12.9 mg/g, 0.1${\sim}$11.4 ${\mu}g/g$를 나타내었다. 퇴적물 입도의 구성은 입경 $74{\mu}m$ 이하가 40.5${\sim}$86.7% 74${\mu}m$ 이상이 11.5${\sim}$43.0%로 나타났다. 등농도 분포도를 이용한 중금속의 공간분포로부터 광양만 퇴적물에 영향을 미치는 몇 곳의 배출원을 추정할 수 있었다. 중금속 배출원 중 여천공단 내의 신풍천, 쌍봉천 그리고 하수처리장의 방류수역이 주요 배출원으로 판정되었다. 중금속의 분석 결과를 US, EPA 비오염해역의 guideline에 비교할 때 납과 수은은 guideline에 모두 적합한 것으로 평가되었으나 망간, 아연, 구리, 철, 비소, 크롬 등은 guideline을 모두 상회하고 있었다. For the purpose of surveying the physicochemical composition of sediment collected from Kwangyang Bay, the percentage of water loss, COD, $H_2S$, grain size and 10 heavy metals were studied at 17 sites. During the surveying period, the changes of the percentage of water loss were appeared $35.5\;{\sim}\;53.8%$. COD and $H_2S$ were showed $3.8\l{\sim}\;12.9\;mg/g$, and $0.1\;{\sim}11.4\;{\mu}g/g$, respectively, In composition of grain size on the sediment, percentages of grain sizes under $74\;{\mu}m$ were varied from 40.5% to 86.7% and above $74\;{\mu}m$ were varied from 11.5% to 43.0%. From the spatial distribution of heavy metal using contour map, we can suppose some heavy metal discharges which affect sediment of Kwangyang Bay, It was estimated that Shinpung creek, Ssang-bong creek, and draining area of sewange treatment plant were the main discharge among the heavy metal output sources. By comparison between present study and heavy metal guideline of nonpolluted sea sediment that is provided by EPA, US, it was showed that the contents of Pb and Hg were acceptable but contents of Mn, Zn, Cu, Fe, As, and Cr were higher than those of EPA guideline.

19-20C 청도 밀양박씨의 향약과 계

박종천 ( Park Jong-chun ) 서울대학교 종교문제연구소 2018 종교와 문화 Vol.0 No.34

The community compacts and mutual aid societies of Milyang Park family in the region of Cheongdo in the 19th and 20th Centuries had showed as follows: (1) The promotions of learning and educational activities of local aristocrats in Joseon dynasty based on the foundation of confucian norms represented how to take control of cultural hegemony in the local area. (2) The community compacts and mutual aid societies of Milyang Park family in the region of Cheongdo built social safety net. And they expended educational activities confined within the relatives, and thereby strengthened the social status of Milyang Park family in the regional community in the 19th through 20th centuries. (3) Janghyun Park inherited the tradition of his family, and thereby developed confucian tradition into national confucianism in modern Korea.

자유표면 유동 시뮬레이션을 위한 고정확도 수치도식의 검토

박종천(JONG-CHUN PARK),이병혁(BYOUNG-HYUK LEE),김정후(JEUNG-HU KIM) 한국해양공학회 2006 韓國海洋工學會誌 Vol.20 No.4

Numerical schemes for spacing and time are tested to accurately simulate the wave propagation. The tested numerical schemesinclude 2nd-order central differencing, 1-order upwind scheme, 2nd-order Leith scheme, 3rd-order MUSCLE, QUICK and QUICKEST schemes in spacing and the Euler and 4th-order Runge-Kutta(R-K) schemes in time. It is seen that more accurate results are expected when the higher-order schemes, especially the schemes combined with a TVD control limiter, are used for solving the wave equation. The 3rd-order upwind scheme with limiter and the 4th-order R-K scheme in time are finally applied to the wave-making simulation in a digital wave tank.

의사소통행위이론과 말씀해석의 공동체

박종천(Jong-Chun Park) 감리교신학대학교 1994 신학과세계 Vol.29 No.-

풍류(風流)로 보는 한국종교의 에토스

박종천 ( Park Jong-chun ) 고려대학교 민족문화연구원 2020 民族文化硏究 Vol.88 No.-

한국의 종교사에서는 불교, 유교, 기독교 등 세계종교의 보편적 이상을 순수하게 추 구하는 주류 엘리트 전통의 ‘순수 정통주의’와 더불어 다양한 이질적 요인들의 혼성적 병존을 드러내는 비주류 대중적 전통의 ‘관용적 포용주의’가 병행되어 왔다. 풍류는 순수 정통주의가 배타적 근본주의로 치닫지 않고 포용적 관용주의와 공존할 수 있게 만든 한국종교의 문화적 에토스였다. 풍류는 ‘현묘지도’(玄妙之道)의 보편적 신비주의의 차원에서 특정한 역사적 상황 속에서 특정한 개별적 종교 전통이 주도적인 가운데 외래에서 유입된 보편적 종교사상들을 개별 종교전통에 충실하게 수용하고 계승하면서도, 열린 자세로 타종교 전통의 문화적 장점을 흡수하여 자기 종교전통을 풍성하게 만들어서 ‘포함삼교’(包含三敎)의 차원에서 다양한 문화를 인정하고 수용하는 문화적 수용성을 통해 종교간 갈등이 증폭되는 배타적 근본주의를 적절하게 제어하는 한편, ‘접화군생’(接化群生)의 사회적 실천을 통해 엘리트 종교사상과 대중적 종교문화 간의 병행도 가능하게 만들었다. 이 글에서는 이러한 관점에 따라 풍류의 에토스가 한국종교 사에서 불교, 유교, 기독교의 핵심 교리나 신념을 일심(一心), 리(理), 씨□ 혹은 얼나 등의 보편적 실재에 입각한 회통(會通)적 사유와 활동을 통해 신비주의적 영성의 보편성으로 전개하고, 이것을 근간으로 삼아 외래의 문화를 수용하는 다문화적 수용성과 함께 사회적 교화를 실현하는 양상을 규명하였다. In the history of Korean religions, the orthodoxy of the mainstream elite tradition pursuing the universal ideals of world religions such as Buddhism, Confucianism and Christianity as well as inclusivism of non-mainstream popular traditions mixing coexistence of various heterogeneous factors have been coexisted. Pungryu (風流) is the Korean religious ethos that made orthodoxy coexisting with inclusivism rather than being driven to exclusive fundamentalism. In the context of universal mysticism, Pungryu has embraced and inherited some cultural merits of other religious traditions in an open attitude while faithfully accepting and inheriting the universal religious ideals that have been introduced from outside in a specific historical context. Through it’s cultural acceptance, which enriches one’s own religious tradition and recognizes and accepts various cultures, it is appropriate to control exclusive fundamentalism that amplifies conflicts between religions, while inclusive social practices enable parallelism between elite religious ideas and popular religious cultures. In this article, following this point of view, I clarified that Pungryu as the religio-cultural ethos based on universal realities such as One heart (一心), Li (理), Spiritual Seed (씨□), and Spiritual I (얼나) developed the universality of mystical spirituality through the thought and activity of harmonization in the history of Korean religions, and materialized social edification along with multicultural acceptance to accommodate foreign cultures.