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김강주,김은철,김기경,이현옥,장선일,정종평,Kim, Kang-Ju,Kim, Eun-Cheol,Kim, Ki-Gyung,Lee, Hyun-Ok,Jang, Seon-Il,Chung, Chong-Pyoung The Korean Academy of Periodontoloy 1997 Journal of Periodontal & Implant Science Vol.27 No.3
헤민과 메나디온 제한에 의한 Porphyromonas gingivalis(P. gingivalis) W50의 병독력의 변화를 검색하고자, 실험관내 병독력을 NIH 3T3 세포의 세포활성 변화로 관찰하였고, 생체내 병독력은 배양상청액을 ICR mouse 피하조직에 주사한 후의 염증반응을 관찰하였다. 헤민 존재 하에 배양한 P. gingivalis W50 배양상청액에 의한 mouse 3T3 세포의 세포활성은 헤민과 메나디온 없이 배양한 세포의 활성보다 낮았다. 헤민과 메나디온을 첨가하지 않고 배양한 세균의 생체내 병독력은 중등도의 염증세포 침윤과 울혈에 의한 출혈, 미약한 세포간질의 부종과 근육 파괴를 보였다. 메나디온 존재 하에서 배양한 세균은 미약한 염증세포의 침윤, 울혈에 의한 출혈 및 근육의 파괴가 관찰되었다. 헤민 존재하에서 중등도의 울혈에 의한 출혈, 미약한 세포간질의 부종, 염증세포의 침윤 및 근육파괴가 관찰되었다. 헤민과 메나디온 존재 하에서 배양한 세균은 심한 염증세포의 침윤과 중등도의 세포간질의 부종 및 울혈에 의한 출혈을 보였다. 이상의 연구 결과 P. gingivalis W50 배양 상층액의 병독력은 헤민에 의하여 영향을 받는 것으로 생각된다.
급성 구강감염부위에서 Staphylococcus lugunensis 의 특성
유용욱,이미성,차정단,김기경,신상희,문상은,김강주 원광대학교 생명공학연구소 1999 생명공학연구소보 Vol.6 No.1
Staphylococcus lugdunensis (S. lugdunensis) is a newly identified pathogenic species of coagulase negative staphylococci and an occasional but not rare cause of severe infections, such as infective endocarditis after dental extraction, bacteremia, osteomyelitis, peritonitis, and soft tissue infections. As antibiotic use increased, resistance rapidly developed. Some strains have plasmids related to antibiotic resistance. To characterize S. lugdunensis in acute oral infection, S. lugdunensis was isolated from the patients with acute oral abscess, osteomyelitis, and normal persons. Antibiotic resistance, in vitro cellular toxicity, in vivo virulence, δ-like hemolysin activity, and synergistic hemolysis on sheep blood agar plates were investigated. The dot blot analysis and Southern blot analysis of staphylococcal DNA was performed with δ-hemolysin gene probe of Staphylococcus aureus. (S. aureus) Staphylococcal DNA was cloned, nucleotide sequence was analysed, and homology was compared with other sequence in Gene Bank. S. aureus, S. lugdunensis, Staphylococcus cohnii (S. cohnii), and other coagulase negative staphylococci (CNS) were isolated from the patients with acute oral infection. The isolation ratio of S. lugdunensis in the patients with infection was higher than that of healthy persons, but the isolation ratio of S. aureus in the patients with infection was similar to that of healthy persons. S. lugdunensis from the patients with acute oral infection showed the resistance to penicillin, methicillin, cephalothin, and clindamycin. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had δ-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with S. aureus on sheep blood agar plates. In dot blot analysis, all strains of S. lugdunensis showed the positive reaction with the probe of δ-hemolysin gene in S. aureus, but a 7.3 kb HindⅢ fragment was observed in the DNA of S. lugdunensis that gave synergistic hemolysis in a Southern blot analysis. The molecular size of partially purified δ-hemolysin was about 50 kd. The cloned fragments from the chromosomal DNA of S. lugdunensis showed the partial homology with the insulin receptor-related and dopamine receptor of humans. These results suggest that S. lugdunensis might be an important pathogen in acute oral infection and show some homology with eukaryotes.
한국인과 일본인에서 분리한 Actinobacillus actinomycetemcomitans의 특성
김강주,김기경,Toshinobu Takemoto,Hidemi Kurihara 원광대학교 치의학연구소 1997 圓光齒醫學 Vol.7 No.2
To compare the characteristics of Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) between Republic of Korea and Japan, A. actinomycetemcomitans was isolated and characterized by the fermentation of carbohydrates, serotype, leukotoxicities, SDS-PAGE, and Western blotting. Biotyping on the basis of the fermentation of xylose, mannitol, and galactose resulted in three distinct biotypes. There are some homologies and heterogeneities of biotypes between Repulic of Korea and Japan. SDS-PAGE and Western blotting patterns of total protein were evaluated for eight strains belonging to the three serotypes. The patterns were similar for all strains except one Japanese isolate investigated. A. actinomycetemcomitans from Japanese patient with localized juvenile periodontitis showed a strong band. All A. actinomycetemcomitans showed the leukotoxicity at 60 min and 90 min. The antibody levels to the serotype a and b of A. actinomycetemcomitans in the Japanese patients with periodontitis were higher than that of serotype c. The antibody levels to A. actinomycetemcomitans were similar among 3 serotypes in Korea. These results suggest that some homologies and heterogeneities of A. actinomycetemcomitans between Republic of Korea and Japan might be existed.
Moon, Sang-Eun,Cha, Joung-Dan,Kim, Ki-Gyung,Lee, Hyun-Ok,Kim, Kang-Ju 원광대학교 생체재료·매식연구소 1997 원광생체재료·매식 Vol.6 No.1
In order to investigate the change of virulence in P. gingivalis W50, in vitro virulence was measured by the change of cell activity in mouse 3T3 cell. The cell activity of cultured supernatant of P. gingivalis W50 in the presence of hemin was lower than that in the obsence of hemin and menadione, but there was no change according to the presence of menadione. These results suggest that the virulence of cultured supernatant in P. gingivalis W50 might be increased by hemin.