유아 교사의 혁신유치원 근무 경험 탐색

강정현(Kang, Jeong-Hyun),권귀염(Kwon, Kyee-Yum) 한국영유아교원교육학회 2021 유아교육학논집 Vol.25 No.2

본 연구는 유아 교사들의 혁신유치원 근무 경험을 탐색하는 것을 목적으로 한다. 혁신유치원에 근무한 경험이 있는 5명의 유아 교사가 연구에 참여하였으며, 자료 수집은 각 참여자들과의 개별 심층 면담 2회, 그룹 면담 1회, 관련 문서자료의 수집을 통해 이루어졌다. 자료 분석 결과, 참여자들은 혁신유치원 근무 경험이 자신을 성찰하고 재도약하는 시간이었으며, 특히 수업 나눔이 도움이 되었다고 하였다. 이들은 소통의 중요성을 깨닫고 다양한 구성원들과 소통을 시도하였으며, 위계적인 현재의 유치원 조직문화에 대해 고민하였다. 이 과정에서 참여자들은 혁신유치원의 성패를 좌우하는 핵심은 제도가 아닌 사람임을 깨닫게 되었다. 또한 혁신유치원 운영 과정에서의 불합리함과 어려움을 경험하면서 문제의식을 느끼고 정책의 진정한 의미 실현에 대해 고민하기도 하였다. 이러한 연구 결과를 바탕으로 교육적 측면, 조직문화 측면, 정책 실행 측면에서의 시사점을 논의하였으며, 후속 연구를 제안하였다. This study aimed to explore early childhood teachers’ experiences in innovative kindergartens. Five early childhood teachers who had experiences in working at innovative kindergartens participated in the study. Data were collected through two in-depth individual interviews with each participant, a group interview, and gathering related documents. The results of data analysis are as follows. By working in the innovative kindergartens, the participant teachers were able to reflect and re-develop themselves as educators; lesson-sharing was particularly helpful. They recognized the importance of communication, and tried to interact with diverse kindergarten members. The hierarchical organizational culture of kindergartens was a point of concern to them. The teachers realized that the success of innovative kindergartens depends on people, not the system. Since the participants became aware of unreasonable aspects of the operating system of innovative kindergartens and experienced difficulties in the work approach, they pondered how to actualize the appropriate direction of innovative kindergartens. Based on the results, implications regarding education, organizational culture, and innovative kindergarten policy were discussed, and suggestions for further research were made.

중소IT기업 S/W 개발인력의 직무몰입 영향요인에 관한 연구

강정현(Kang, Juong-Hyun),정대율(Jeong, Dae-Yul) 한국산학기술학회 2012 한국산학기술학회논문지 Vol.13 No.8

본 논문의 목적은 중소 IT기업 S/W 개발자의 생산성을 높이기 위하여 어떤 요소들이 직무몰입에 긍정 또는 부정적 영향을 미치는가를 분석하는데 있다. 본 연구에서는 개발자의 직무능력을 중요한 선행변수로 보고 있다. 그리 고 역할갈등과 태도가 직무몰입을 가져오는 중요한 매개 작용을 한다는 가설에서 출발하였다. 실증자료를 통한 경로 분석 결과, 직무능력 변수들이 매개변수인 역할갈등과 태도에 영향을 미치며, 이들이 직무몰입에 직, 간접적으로 영향 을 미치는 것으로 나타났다. 특히 중요한 발견은 업무지식이 높을수록 역할갈등은 낮게 나타났으며, 업무기술력이 높 은 인력일수록 역할갈등이 더 높게 나타났다. 이는 중소 IT기업의 경우 조직 내에서 직무기술력이 우수할수록 많은 기대와 일을 시키는 것에서 기인되었다고 보아진다. 결국, IT 개발자의 업무지식을 높이고 직무에 대하여 좋은 태도 를 형성하여야 직무몰입을 높일 수 있다. The purpose of this paper is to find the factors that affect on the job involvement of S/W developer in the small and medium IT companies. We suggested structural model with two mediating variables, role conflicts and attitude of workers. The model considered job competency(job knowledge, technological competences, and communication skill) as independent variables which affect on the mediating variables directly and final dependent variable indirectly. The hypotheses tests showed that the job knowledge affected negatively on the role conflicts as the hypothesis assumed, but technological competences affected positively(reserve direction) on the role conflicts. This finding means that the more he / she has good job skill and technologies, the more does he required to do many things in the organization. The results of path analysis showed that attitude of workers mediated between the role conflicts and job involvement significantly. We concluded that although the workers are suffered from the role conflicts, it could be mitigated by their job knowledge and the favorite attitude toward their job, and finally they could more involve in their job.

방실결절회귀성 빈맥의 성공적인 전극도자 절제의 예측인자

강정채(Jung Chaee Kang),정명호(Myung Ho Jeong),조장현(Jang Hyun Cho),김성희(Sung Hee Kim),안영근(Young Keun Ahn),박주형(Joo Hyung Park),조정관(Jeong Gwan Cho),박종춘(Jong Chun Park),이상현(Sang Hyun Lee),김준우(Jun Woo Kim) 대한내과학회 1999 대한내과학회지 Vol.57 No.5

N/A Catheter ablation of the AV nodal slow pathway using radiofrequency (RF) energy has been established as the first-line curative therapeutic modality of recurrent symptomatic AV nodal reentrant tachycardia (AVNRT). In contrast to catheter ablation of the AV bypass tract, there was no useful marker to localize succesful site of the pathway. This study was performed to determine predictors of successful catheter ablation of the AV nodal slow pathway in patients with AVNRT. Methods : Forty patients (18 men, 22 women; 47.9±13.3 years) with AVNRT undergoing successful catheter ablation of the AV nodal slow pathway were included in this study, in which 217 attempts were tried to ablate the AV nodal slow pathway. Characteristics of local atrial electrogram, anatomical site at each attempt, junctional rhythm during RF delivery were analyzed (40 successful, 177 failed). Maximum difference and duration of atrial electrograms were measured and local atrial electrograms were classified into 5 types (A1, A2, B1, B2 and C type) according to the type and the degree of fragmentation. Finally, the occurrence of junctional rhythm during RF discharge and its onset time were compared between successful and failed attempts. Results : There was no significant difference in the maximum difference of amplitude and duration of atrial electrograms between successful and failed attempts. The success rate in each type of atrial electrogram was significantly different. And, the success rate in non-C type atrial electrograms (A1, A2, B1, and B2) was significantly higher than that in type C atrial electrograms (25.0% vs 10.3%, p<0.01). No significant difference was noted in success rates according to attempted sites. Junctional rhythms during radiofrequency application occured significantly more frequent in successful attempts than in failed attempts (87.5% vs 47.5%, p<0.001). The time to onset of junctional rhythm was not different between successful and failed attempts (5.2±4.9 sec vs 6.1±5.5 sec). Conclusion : Fragmented local atrial electrogram and junctional rhythm during RF energy delivery may be used to predict successful catheter ablation of AVNRT. It is recommended that RF energy should be applied to the site where fragmented atrial electrogram is recorded and terminated if junctional rhythm does not develop within 15 seconds after starting RF energy delivery. (Korean. J. Med 57:867-874, 1999)

헤파린 부착 관상동맥 스텐트의 스텐트 재협착 예방에 대한 효과

강정채(Jung Chaee Kang),박창수(Chang Soo Park),정명호(Myung Ho Jeong),조장현(Jang Hyun Cho),김성희(Sung Hee Kim),안영근(Young Keun Ahn),박주형(Joo Hyung Park),조정관(Jeong Gwan Cho),박종춘(Jong Chun Park),정상우(Sang Woo Juhng),김준 대한내과학회 1999 대한내과학회지 Vol.57 No.1

N/A The coronary stent reduces acute coronary arterial occlusion and late restenosis during and after coronary intervention. However, stent thrombosis and restenosis are still major limitations in widespread use of coronary stent. Local drug delivery with use of heparin-coated stent will be a new approach reducing the incidence of stent thrombosis and restenosis. In order to evaluate the effects of heparin-coated stent on stent restenosis, heparin-coated stents were compared with control stents in a porcine coronary stent restenosis model. Methods : Stent overdilation injury (stent:artery= 1.3:1.0) was performed with bare Wiktor (Group I, n=10) and heparin-coated Wiktor (Group II, n=20) stents (HEPAMEDTM, Medtronics, U.S.A.) in porcine coronary arteries. Follow-up quantitative coronary angiography (QCA) was performed at 4 weeks after stenting and histopathologic assessments of stented porcine coronary arteries were compared in both groups. Results : 1) On QCA, percent diameter stenosis was significantly higher in Group I than in Group II (16.3±6.62% vs. 9.6±5.06%, p<0.05). 2) Injury score of stented porcine coronary artery was not different in both groups (1.26±0.23 vs. 1.20±0.22). 3) Pathologic area stenosis of stented artery was higher in Group I than in Group II (41.6±12.5% vs. 27.1±9.9%, p<0.005). 4) Neointimal area was higher in Group I than in Group II (4.58±1.41 mm2 vs. 2.57±1.07 mm2, p<0.05). 5) By immunohistochemistry, proliferating cell nuclear antigen (PCNA) index was higher in Group I compared with in Group II (11.2±6.75% vs. 6.3±4.14%, p<0.05). Conclusions : Heparin-coated stent is effective in the prevention of late coronary stent restenosis in a porcine coronary stent restenosis model, which may be related with the inhibition of neointimal cell proliferation.

IT 벤처기업의 종업원의 역할갈등이 직무몰입과 직무만족에 미치는 영향에 관한 연구

강정현(Jung hyun Kang),정대율(Dae yul Jeong) 한국창업학회 2012 한국창업학회지 Vol.7 No.2

Recently, the demand of mobile applications increased explosively, the IT venture companies in Korea, more than 90% of them are small and medium enterprise, have difficulty in the personnel management due to the frequent turnover of the his workforce. Most of employees of IT venture company who are expert in information technology and system development are suffering from role conflicts due to the over expectancy of his/her supervisor and work overloading. Many researchers have been proved that the role conflicts influence on the job performance and job satisfaction negatively. That s why most employees leave the organization or turn over the job. We propose a research model that hypothesize the relationship among the role conflicts, job involvement, and job satisfaction, and also the leadership will moderate the role conflicts and the dependent variables, job involvement and job satisfaction. The results of the hypothesis test were some different from the previous researches, in which, the role conflicts affected to the job satisfaction significantly but there was no evidence that the role conflicts affect to the job involvement. So, the model needed more explanatory variables, such as leadership to explain our research domain situation. We classified the leadership as three type, servant, transactional, and transformational leadership. We are interest in which leadership type is more effective in moderating the role conflicts. The empirical test showed that the servant leadership was most effective, but transactional leadership wasn t effective. The transformational leadership moderated effectively to the job satisfaction, but wasn t to the job involvement. From the hypothesis tests, we could derive some important implications to the IT personnel management.

돼지 관상동맥 스텐트 재협착에 대한 Endothelin 수용체 차단제 국소 전달요법의 효과

김진우(Jin Woo Kim),강정채(Jung Chaee Kang),박창수(Chang Soo Park),김완(Wan Kim),정명호(Myung Ho Jeong),김용록(Yong Rok Kim),조장현(Jang Hyun Cho),김주한(Ju Han Kim),김성희(Sung Hee Kim),배열,안영근(Young Keun Ahn),박주형(Joo Hyung Pa 대한내과학회 1999 대한내과학회지 Vol.56 No.6

N/A Objectives : Coronary stent is one of the most effective currently available devices in the treatment of coronary artery diseases. But, coronary stent restenosis is one of major limitations in clinical stenting. Local drug delivery may be a new strategy for the prevention of stent restenosis. Endothelin receptor blocker is known to have vasodilatory and antiproliferative activities. To investigate the effects of local endothelin receptor blocker delivery on stent restenosis, local delivery was performed in the porcine model of coronary stent restenosis. Methods : Stent overdilation injury alone was performed in the control porcine coronary arteries (n=4, group A) and local delivery of endothelin receptor blocker prior to stenting was performed in the porcine coronary artery (n=9, group B). Endothelin receptor blocker (TAK-044, Takeda, Japan) was delivered at a rate of 1 ml/min (50 mg/10 ml) using the Dispatch Catheter. Follow-up quantitative coronary angiogram (QCA) and immunohistopathologic assessment were performed 4 weeks after stenting. Results : 1) On QCA, percent diameter stenosis was significantly higher in Group A than in Group B (29.4±6.1 % vs. 14.5±11.6%, p<0.05). 2) Area stenosis was higher in Group A than in Group B (63.5±23.2 % vs. 40.9±13.3 %) measured by histopathologic method (p<0.05). 3) Neointimal area was higher in Group A than in Group B (3.53±1.9 mm2 vs. 1.75±0.8 mm2, p=0.03). 4) By immunocytochemistry, proliferating cell nuclear antigen index was higher in Group A compared with Group B (46.8±5.2 % vs. 31.1±3.7 %, p<0.05). Conclusion : Local delivery of endothelin receptor blocker is effective in the prevention of stent restenosis in a porcine model, which may be related with the partial inhibition of cell proliferation of neointimal cells.

Salmonella typhimurium에서 유래한 Mannitol Dehydrogenase 유전자의 대장균 내 발현 및 효소특성 규명

장명운,박정미,김민정,강정현,이소원,김태집,Jang, Myoung-Uoon,Park, Jung-Mi,Kim, Min-Jeong,Kang, Jung-Hyun,Lee, So-Won,Kim, Tae-Jip 한국미생물학회 2012 미생물학회지 Vol.48 No.2

Salmonella typhimurium LT2 (KCTC 2421)로부터 mannitol dehydrogenase (StMDH)로 추정되는 유전자를 클로닝하고, 대장균에서 대량 발현하였다. 이 유전자는 488개의 아미노산 서열(약 54 kDa)을 암호화하는 1,467 bp의 염기로 구성되며, 이미 보고된 long-chain dehydrogenase/ reductase (LDR) 계열 효소들과 약 36%의 아미노산 서열 상동성을 나타내었다. 재조합 StMDH의 최적 반응온도는 $30^{\circ}C$이며, pH 5.0의 조건에서 최대의 D-fructose 환원활성, 그리고 pH 10.0에서 최대의 D-mannitol 산화활성을 보인다. 반면에 glucose, galactose, xylose, arabinose 등의 기질에 대해서는 활성을 보이지 않았다. 이 효소는 $NAD^+$/NADH 존재 하에서만 산화 환원 활성을 가지며, $NADP^+$/NADPH는 조효소로 이용하지 못하였다. 결론적으로 StMDH는 전형적인 $NAD^+$/NADH 의존형의 mannitol dehydrogenase (EC 1.1.1.67)임을 확인하였다. A mannitol dehydrogenase (StMDH) gene was cloned from Salmonella typhimurium LT2 (KCTC 2421) and overexpressed in Escherichia coli. It has a 1,467 bp open reading frame encoding 488 amino acids with deduced molecular mass of 54 kDa, which shares approximately 36% of amino acid identity with known long-chain dehydrogenase/reductatse (LDR) family enzymes. The recombinant StMDH showed the highest activity at $30^{\circ}C$, and pH 5.0 and 10.0 for D-fructose reduction and D-mannitol oxidation, respectively. On the contrary, it has no activity on glucose, galactose, xylose, and arabinose. StMDH can catalyze the oxidative/reductive reactions between D-fructose and D-mannitol only in the presence of $NAD^+$/NADH as coenzymes. These results indicate that StMDH is a typical $NAD^+$/NADH-dependent mannitol dehydrogenase (E.C. 1.1.1.67).

수술전 화학방사선 요법을 시행 받은 직장암에서 혈중 암종배아항원과 조직학적 치료반응도와의 상관관계분석

심홍진 ( Hong Jin Shim ),강정현 ( Jeong Hyun Kang ),이형순 ( Hyung Soon Lee ),허혁 ( Hyuk Hur ),민병소 ( Byung Soh Min ),이강영 ( Kang Young Lee ),김남규 ( Nam Kyu Kim ),김영완 ( Young Wan Kim ) 대한임상종양학회 2010 Korean Journal of Clinical Oncology Vol.6 No.1

목적 : 본 연구는 수술 전 화학방사선요법을 시행 받고 수술을 시행한 직장암 환자에서 조직학적 반응도와 혈청 암종배아항원의 상관관계를 알아보기 위해 시행되었다. 방법 : 2005년 8월부터 2007년 12월까지 연세대학교 세브란스 병원에서 직장암으로 진단 받고 수술 전 화학방사선요법을 시행받은 후 전직장간막 절제술을 시행한 85명의 환자를 대상으로 하였다. 화학요법은 5-Fluorouracil에 기초하였고 방사선주사는 총 5,040 cGy 를 조사하였다. 암종배아항원은 화학 방사선요법전(pre-CRT)과 직후(post-CRT), 수술 후 7일째(post-TME)에 각각 측정을 하였다. 조직학적 반응도는 TNM 병기에 따라 반응군(Favorable response : pCR 과ypStage1, 28명)과 비반응군(Unfavorable response : ypStage2와 ypStage3, 57명)으로 구분하였다. 결과 : 환자의 나이, 성별, 암의 위치, 림프혈관의 침범, 신경주위침범 등에서 반응군과 비반응군의 차이는 없었다. 반응군에서 low grade 의 조직학적 분화를 보이는 비율이 높았고(92% vs.70.2%, p=0.018) 항문보존술식을 시행한 비율이 더 높았다(92.9% vs. 71.9%, p=0.026). pre-CRT CEA수치는 반응군에서 유의하게 낮았으나(p<0.001) post-CRT, post-TME CEA값은 두 군간의 차이는 없었다. 다변량 분석에서 낮은 pre-CRT CEA(<5ng/ml) 값과 항문보존술식의 시행여부가 조직학적 반응군과 관련된 독립적인 인자로 확인되었다. 결론 : 직장암 환자에서 화학방사선치료 전 CEA 수치는 수술 후 조직학적 반응정도와 상관관계를 보였으나 화학방사선치료 후나 수술 후의 CEA값은 조직학적 반응정도와 상관 관계가 없었다. 이러한 결과를 바탕으로 CEA가 조직학적 반응정도를 예측하는 인자로서의 역할을 할 수 있는지에 대한 향후 전향적인 연구가 필요하다. Purpose: This study was designed to assess whether serum CEA is associated with pathological tumor response in rectal cancer patients who underwent preoperative chemoradiation therapy (CRT) with total mesorectal excision (TME). Methods: Eighty-five patients with rectal cancer who were treated by preoperative CRT followed by TME were enrolled between August 2005 and December 2007. 5-FU based chemotherapy and 5040 cGy of radiation were delivered. Serum CEA was measured pre-CRT, post-CRT, and post-TME period. Among 85 patients, 29 patients did not have post-CRT CEA level. Pathological tumor response (ypTNM stage) was categorized into two groups as follows; favorable response group (group A: n=28, pathological complete response and ypTNM I) vs unfavorable response group (group B: n=57, ypTNM II and III). Median follow-up period was 29.2 months (range 1.1-50.2 months). Results: There were no differences between favorable and unfavorable response group with respect to age, gender, tumor location, lymphovascular invasion, and perineural invasion (Table 1). Anal sphincter preservation surgery was more commonly performed in the group A when compared with group B (26 (92.9%) vs. 41 (71.9%)) (p=0.026). Well and moderately differentiated histology were more commonly found in the group A (26(92.9%) vs. 40(70.2%) (p=0.018). Low level of pre-CRT CEA (<5ng/ml) was more commonly found in the group A (26(92.9%) vs. 30 (52.6%) (p=0.000). However, there was no difference between group A and B with regard to post-CRT CEA and post-TME CEA. Logistic regression analyses showed that pre-CRT CEA (<5ng/ml) and sphincter preservation surgery were associated with favorable pathological tumor response. Conclusions: Low level of pre-CRT CEA (<5ng/ml) is predictive of favorable pathological tumor response but serum level of post-CRT and post-TME CEA did not have significant association with tumor response. This result should be validated in larger prospective randomized study near future.

에너지관리 측면에서의 Nonintrusive load monitoring의 적용성 검토를 위한 건축물 및 에너지 통계조사

곽영훈(Young Hoon Kwak),강정아(Jeong-A Kang),김도운(Do Un Kim),이수현(Su Hyun Lee),황지현(Ji Hyun Hwang),이태원(Tae Won Lee) 대한설비공학회 2018 대한설비공학회 학술발표대회논문집 Vol.2018 No.6

Lactococcus lactis subsp. lactis 유래 cyclomaltodextrinase 유전자의 대장균 내 발현 및 효소 특성

장명운 ( Myoung Uoon Jang ),강혜정 ( Hye Jeong Kang ),정창구 ( Chang Ku Jeong ),박정미 ( Jung Mi Park ),이아름 ( Ah Rum Yi ),강정현 ( Jung Hyun Kang ),이소원 ( So Won Lee ),김태집 ( Tae Jip Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.4

본 연구에서 584개의 아미노산(68.7 kDa)으로 구성된 cyclomaltodextrinase (LLCD)의 유전자를 Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435)로부터 클로닝하였다. LLCD는 일반적인 CDase 계열 효소들과 약 40% 전후의 아미노산 서열 상동성을 나타내었다. C-말단에 6개의 히스티딘 잔기를 가진 재조합 효소는 dimer의 형태로 대장균에서 발현되고 정제되었다. LLCD는 pH 7.0 및 37oC에서 최대의 β-CD 가수분해 활성을 나타내었다. 특히, 이 효소는 starch 및 pullulan에 대해 극히 낮은 활성을 보였으나, 반면에 CD에 대한 가수분해 활성은 starch에 비해 약 80배 이상 높았다. 이처럼 높은 CD에 대한 활성을 근거로 LLCD는 CDase 계열 효소로 분류될 수 있으나, starch, pullulan, 그리고 acarbose에 대한 매우 낮은 활성은 다른 유사효소와 비교하여 차별화되는 특징이다. A putative cyclomaltodextrinase (LLCD) gene was cloned from the genome of Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435), which encodes 584 amino acids with the predicted molecular mass of 68.7 kDa. KCTC 3769 shares approximately 40% of amino acid sequence identity with the CDase-family of enzymes. The dimeric enzyme with C-terminal six-histidines was heterologously expressed and purified from recombinant E. coli. LLCD showed the highest activity against β- cyclodextrin (CD) at pH 7.0 and 37oC. In particular, LLCD exhibited extremely low activity against starch and pullulan, while its CD-hydrolyzing activity was about 80 times higher than starch. Due to its much higher activity on CD over starch, LLCD has been identified as a member of CDases. However, LLCD can be distinguished from the other common CDases on the basis of its extremely low hydrolyzing activity against starch, pullulan, and acarbose.