Anti-inflammatory effect of hispidin on LPS induced macrophage inflammation through MAPK and JAK1/STAT3 signaling pathways

Han Ying-Hao,Chen Dong-Qin,Jin Mei-Hua,Jin Ying-Hua,Li Jing,Shen Gui-Nan,Li Wei-Long,Gong Yi-Xi,Mao Ying-Ying,Xie Dan-Ping,Lee Dong-Seok,Yu Li-Yun,Kim Sun-Uk,김지수,권태호,Cui Yu-Dong,Sun Hu-Nan 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

Severe inflammatory reactions caused by macrophage activation can trigger a systemic immune response. In the present study, we observed the anti-inflammatory properties of hispidin on LPS induced RAW264.7 macrophage cells. Our results showed that hispidin treatment significantly reduced the production of cellular NO, IL-6 and reactive oxygen species (ROS) while has not inhibitory effect on TNF-α productions. Excitingly, hispidin treatment retains the phagocytosis ability of macrophages which enabling them to perform the function of removing foreign invaders. Signaling studies showed, hispidin treatment dramatic suppressed the LPS induced mitogen activated protein kinases (MAPK) and JAK/STAT activations. In conclusion, our findings suggest that hispidin may be a new therapeutic target for clinical treatment of macrophages-mediated inflammatory responses.

한국의 산업별 주가와 주요거시변수간의 관계추정

( Ying Ying Han ),전승국 ( Seung Kug Jheon ),김영재 ( Young-jae Kim ) 한국경제통상학회(구 한국경상학회,한국국민경제학회) 2015 경제연구 Vol.33 No.1

본 연구에서 산업별 주가와 거시경제변수사이의 동태적 조건부 상관관계를 도출하였다. 또한 동태적 상관성과 함께 주요거시경제변수(WTI, CPI, IP, IR)들이 어떤 영향을 미치는지를 분석하기 위해 Johansen 공적분 검정과 VECM 모형을 통한 충격반응함수(impulse response function)를 통해 분석하였으며, 공적분 검정을 실시한 결과 내구재산업과 비내구재 산업 모두에서 공적분이 존재하였다. 그리고 충격반응함수 분석 결과 거시경제변수에 대한 영향은 내구재가 비내구재에 비해 상대적으로 크게 나타났다. 그 중에서도 이자율(IR)에 대한 반응은 모든 변수에 대해서 양(+)의 반응을 보이고, 소비자물가지수(CPI)에 대한 반응은 비내구재 산업의 통신(TEL)과 필수소비재(ECG)에서 음(-)의 반응이 나타났으나, 타 산업에 대해서는 완만한 양(+)의 반응이거나 음(-)의 반응에서 양(+)의 반응으로 반전하는 모습을 보여주고 있다. 또한 산업생산지수(IP)는 초기에는 급격한 반응을 보였으나 시간이 지날수록 완만한 양(+)의 값으로 수렴해주는 것을 보여주고 있다. 유가와 이자율이 상승하게 되면 시장에 유동성이 감소하여 주식수익률이 하락할 수 있다는 것을 보여주었다. This paper analyzes the relationships between stock market indexes by industries and macroeconomic variables such as international oil price (WTI), consumer price index (CPI), interest rate (IR) and industrial production (IP) by VECM. The main outcomes based on cointergration test show that there exists cointergration in all variables excluding telecommunication industry in non-durable goods markets, which means that there exist long-run equilibrium relations between the corresponding variables. The results of VECM represent that the variables in durable goods markets are more sensitive than those in non-durable goods markets, and the IR turns out the most influential variable in the VECM outcomes. This result coincides with the fact that sectoral-industrial return can be reduced due to the decreased liquidity in the market if IR increases.

RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Han Ying-Hao,Mao Ying-Ying,Yu Nan-Nan,Jin Mei-Hua,Jin Ying-Hua,Wang Ai-Guo,Zhang Yong-Qing,Shen Gui-Nan,Cui Yu-Dong,Yu Li-Yun,Lee Dong-Seok,Jo Yu-Jin,Sun Hu-Nan,Kwon Jeongwoo,권태호 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII+/+ (WT) and PrxII−/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII−/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII−/− vs. PrxII+/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

Identification of peroxiredoxin II and its related molecules as potential biomarkers of dermal mesenchymal stem cell homing using network analysis

Han Ying-Hao,Mao Ying-Ying,Feng Yao-Yuan,Xiang Hong-Yi,Sun Hu-Nan,Jin Mei-Hua,Kwon Taeho 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.3

In this study, we performed RNA sequencing of Prx II +/+ and Prx II −/− dermal mesenchymal stem cells (DMSCs) to identify differentially expressed genes (DEGs). To explore the role of Prx II in DMSCs, we performed Gene Ontology analysis of the DEGs. The results showed that the DEGs were mainly involved in the biological processes of cell migration, intercellular adhesion, and coordination of the regulation of stem cell homing. Through the construction of protein–protein interaction network, four hub genes Cd274 , Ccl5 , Il1b , and Stat1 involved in cell adhesion and cell homing were screened. Quantitative reverse transcription PCR analysis showed that Cd274 , Ccl5 , Il1b , and Stat1 were down regulated in Prx II −/− DMSCs. miRwalk and Starbase databases were further used to screen the upstream molecules miRNA and lncRNA regulating hub gene. Prx II was found to be involved in the regulation of stem cell homing via the Tctn2/miR-351/Stat1/Il1b axis. Thus, we demonstrated that Prx II is a key molecule in the regulation of the homing ability of DMSCs. Our results provide a theoretical foundation for improving the homing ability of DMSCs by targeting Prx II.

MUTYH Association with Esophageal Adenocarcinoma in a Han Chinese Population

Kong, Feng,Han, Xue-Ying,Luan, Yun,Qi, Tong-Gang,Sun, Chao,Wang, Jue,Hou, Hua-Ying,Jiang, Yu-Hua,Zhao, Jing-Jie,Cheng, Guang-Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Adenocarcinoma of esophagus (AE) is a complex disease, affected by a variety of genetic and environmental factors. Much evidence has shown that the MutY glycosylase homologue (MUTYH) plays a key role in the pathogenesis of many cancers. However, there have been no reports on influence on AE in the Han Chinese population. The objective of this study was to investigate this issue. A gene-based association study was conducted using three single nucleotide polymorphisms(SNPs) reported in previous studies. The three SNPs (rs3219463, rs3219472, rs3219489) were genotyped in 207 unrelated AE patients and 249 healthy controls in a case-control study using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The results revealed that the genotype distribution of rs3219472 differed between the case and control groups (OR=1.66,95%CI=1.11-2.48, P=0.012), indicating that an association may exist between MUTYH and AE. These findings support a signifcant role for MUTYH in AE pathogenesis in the Han Chinese population.

Regulatory effect of peroxiredoxin 1 (PRDX1) on doxorubicin-induced apoptosis in triple negative breast cancer cells

Han Ying-Hao,Lian Xu-Dong,Lee Seung-Jae,Li Wei-Long,Sun Hu-Nan,Jin Mei-Hua,Kwon Taeho 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5

Patients with triple negative breast cancer (TNBC) lack the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2; thus, conventional hormone and targeted therapies have minimal effect on them. Therefore, clinical treatment of TNBC is still based on chemotherapy and supplemented by other methods. Doxorubicin (DOX), a common drug used in TNBC chemotherapy, has high affinity for cardiolipin, and the nematosomes are rich in cardiolipin; therefore, DOX has high mitochondria-targeting ability. DOX accumulates and plunders the electrons of nicotinamide adenine dinucleotide phosphate (NADPH) and cytochrome C in mitochondria to produce semiquinone DOX. Under the action of oxygen molecules, semiquinone DOX is reduced to DOX and reactive oxygen species (ROS) are generated. The accumulation of ROS can cause mitochondrial dysfunction and lead to mitochondrial dependent apoptosis. Bioinformatic analysis of samples from TNBC patients revealed that peroxiredoxin 1 (PRDX1) was highly expressed in TNBC tissues, and the poor prognosis of patients with high PRDX1 expression was considerably increased. Previous studies determined that DOX can upregulate the expression of the PRDX1 protein in the human TNBC cell line (MDA-MB-231). Thus, we speculate that PRDX1 plays an important role in the process of DOX-induced TNBC cell apoptosis. In this study, we aimed to explore the role of PRDX1 in the process of DOX-induced TNBC cell apoptosis. We found that PRDX1 deletion increased the sensitivity of MDA-MB-231 cells to DOX, which was mainly due to mitochondrial oxidative stress caused by intracellular ROS accumulation, leading to mitochondriadependent apoptosis. Deletion of PRDX1 promotes the PI3K/Akt signaling pathway to mediate the expression of GSK3β. Gsk3β is an upstream signal of mitochondria-dependent apoptosis, and is also an important target of ROS. PRDX1 participates in adriamycin-induced apoptosis of TNBC cells by regulating the expression level of GSK3β. Our findings present new insights to treat breast cancer and TNBC, outlines the clinical use of DOX, and provides a basic theory to develop PRDX1 gene function.

Convergence of weighted sums for dependent random variables

Han-Ying Liang,Dong-Xia Zhang,Jong-Il Baek 대한수학회 2004 대한수학회지 Vol.41 No.5

We discuss in this paper the strong convergence for weighted sums of negative associated (in abbreviation: NA) arrays. Meanwhile, the central limit theorem for weighted sums of NA variables and linear process based on NA variables is also considered. As corollary, we get the results on iid of Li et al. ([10]) in NA setting.

A Berry-Esseen type bound of regression estimator based on linear process errors

Han-Ying Liang,Yu-Yu Li 대한수학회 2008 대한수학회지 Vol.45 No.6

Consider the nonparametric regression model Yni = g(xni)+ ∈ni (1 ≤ i ≤ n), where g(·) is an unknown regression function, xni are known fixed design points, and the correlated errors {∈ni, 1 ≤ i ≤ n} have the same distribution as {Vi, 1 ≤ i ≤ n}, here Vt = [수식] with [수식] and {et} are negatively associated random variables. Under appropriate conditions, we derive a Berry-Esseen type bound for the estimator of g(·). As corollary, by choice of the weights, the Berry- Esseen type bound can attain O(n−¹/⁴(log n)³/⁴). Consider the nonparametric regression model Yni = g(xni)+ ∈ni (1 ≤ i ≤ n), where g(·) is an unknown regression function, xni are known fixed design points, and the correlated errors {∈ni, 1 ≤ i ≤ n} have the same distribution as {Vi, 1 ≤ i ≤ n}, here Vt = [수식] with [수식] and {et} are negatively associated random variables. Under appropriate conditions, we derive a Berry-Esseen type bound for the estimator of g(·). As corollary, by choice of the weights, the Berry- Esseen type bound can attain O(n−¹/⁴(log n)³/⁴).

Systematic Review on Epstein-Barr Virus (EBV) DNA in Diagnosis of Nasopharyngeal Carcinoma in Asian Populations

Han, Bao-Lin,Xu, Xiang-Ying,Zhang, Chun-Zhi,Wu, Jian-Juan,Han, Chun-Feng,Wang, Hui,Wang, Xuan,Wang, Guang-Shun,Yang, Shu-Juan,Xie, Yao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To conduct a meta-analysis to investigate the value of EBV DNA in diagnosis of nasopharyngeal cancer (NPC) in Asian populations, and provide important evidence for screening. Methods: Prospective or respective case-control or cohort studies regarding the detection role of EBV DNA for NPC were included in our study. We conducted a comprehensive literature search in PubMed, EMBASE, and the Chinese Biomedical Database (CBM database between January 1980 and March 2012. Results: A total of 18 studies with 1492 NPC cases and 2641 health controls were included. Almost of the included studies were conducted in China, and only one other conducted in Thailand. The overall results demonstrated that the pooled sensitivity, specificity, positive likelihood (+LR) and negative likelihood (-LR) were 0.73 (0.71-0.75), 0.89 (0.88-0.90), 8.84 (5.65-13.84) and 0.19(0.11-0.32), respectively. The overall EBV DNA detection showed the largest area of 0.932 under the summary receiver operator curve (SROC). The accuracy of detection by plasma for NPC (0.86) was higher than in serum (0.81), with largest areas under the SROC of 0.97 and 0.91, respectively. Conclusion: Our results demonstrated the EBV DNA detection in plasma or serum has high sensitivity and specificity in diagnosis of NPC, especially in Chinese populations with a high risk of cancer.

Induction of cellular senescence in sestrin 2 deficient mouse embryonic fibroblast via accumulation of ROS

Ying-Hao Han,Ki-Sun Kwon,Dae-Yeul Yu 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.8