Neutrophil Count and the Inflammation-based Glasgow Prognostic Score Predict Survival in Patients with Advanced Gastric Cancer Receiving First-line Chemotherapy

Li, Qing-Qing,Lu, Zhi-Hao,Yang, Li,Lu, Ming,Zhang, Xiao-Tian,Li, Jian,Zhou, Jun,Wang, Xi-Cheng,Gong, Ji-Fang,Gao, Jing,Li, Jie,Li, Yan,Shen, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Purpose: To explore the value of systemic inflammatory markers as independent prognostic factors and the extent these markers improve prognostic classification for patients with inoperable advanced or metastatic gastric cancer (GC) receiving palliative chemotherapy. Methods: We studied the prognostic value of systemic inflammatory factors such as circulating white blood cell count and its components as well as that combined to form inflammation-based prognostic scores (Glasgow Prognostic Score (GPS), Neutrophil-Lymphocyte Ratio (NLR), Platelet Lymphocyte Ratio (PLR), Prognostic Index (PI) and Prognostic Nutritional Index (PNI)) in 384 patients with inoperable advanced or metastatic gastric cancer (GC) receiving first-line chemotherapy. Univariate and multivariate analyses were performed to examine the impact of inflammatory markers on overall survival (OS). Results: Univariate analysis revealed that an elevated white blood cell, neutrophil and/or platelet count, a decreased lymphocyte count, a low serum albumin concentration, and high CRP concentration, as well as elevated NLR/PLR, GPS, PI, PNI were significant predictors of shorter OS. Multivariate analysis demonstrated that only elevated neutrophil count (HR 3.696, p=0.003) and higher GPS (HR 1.621, p=0.01) were independent predictors of poor OS. Conclusion: This study demonstrated elevated pretreatment neutrophil count and high GPS to be independent predictors of shorter OS in inoperable advanced or metastatic GC patients treated with first-line chemotherapy. Upon validation of these data in independent studies, stratification of patients using these markers in future clinical trials is recommended.

Lung-Targeting Delivery of Dexamethasone Acetate Loaded Solid Lipid Nanoparticles

Qing-yu Xiang,Yuan Huang,Zhi-rong Zhang,Min-ting Wang,Fu Chen,Tao Gong,Yan-lin Jian 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.4

The objective of the present study was to develop a novel solid lipid nanoparticle (SLN) for the lung-targeting delivery of dexamethasone acetate (DXM) by intravenous administration. DXM loaded SLN colloidal suspensions were prepared by the high pressure homogenization method. The mean particle size, drug loading capacity and drug entrapment efficiency (EE %) of SLNs were investigated. In vitro drug release was also determined. The biodistribution and lung-targeting efficiency of DXM-SLNs and DXM-solutions (DXM-sol) in mice after intravenous administration were studied using reversed-phase high-performance liquid chromatography (HPLC). The results (expressed as mean ± SD) showed that the DXM-SLNs had an average diameter of 552 ± 6.5 nm with a drug loading capacity of 8.79 ± 0.04% and an entrapment efficiency of 92.1 ± 0.41%. The in vitro drug release profile showed that the initial burst release of DXM from DXM-SLNs was about 68% during the first 2 h, and then the remaining drug was released gradually over the following 48 hours. The biodistribution of DXM-SLNs in mice was significantly different from that of DXM-sol. The concentration of DXM in the lung reached a maximum level at 0.5 h post DXM-SLNs injection. A 17.8-fold larger area under the curve of DXM-SLNs was achieved compared to that of DXM-sol. These results indicate that SLN may be promising lung-targeting drug carrier for lipophilic drugs such as DXM.

Lung-Targeting Delivery of Dexamethasone Acetalte Loaded Solid Lipid Nanoparticles

Xiang, Qing-Yu,Wang, Min-Ting,Chen, Fu,Gong, Tao,Jian, Yan-Lin,Zhang, Zhi-Rong,Huang, Yuan 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.4

The objective of the present study was to develop a novel solid lipid nanoparticle (SLN) for the lung-targeting delivery of dexamethasone acetate (DXM) by intravenous administration. DXM loaded SLN colloidal suspensions were prepared by the high pressure homogenization method. The mean particle size, drug loading capacity and drug entrapment efficiency (EE %) of SLNs were investigated. In vitro drug release was also determined. The biodistribution and lung-targeting efficiency of DXM-SLNs and DXM-solutions (DXM-sol) in mice after intravenous administration were studied using reversed-phase high-performance liquid chromatography(HPLC). The results (expressed as mean ${\pm}$ SD) showed that the DXM-SLNs had an average diameter of 552 ${\pm}$ 6.5 nm with a drug loading capacity of 8.79 ${\pm}$ 0.04% and an entrapment efficiency of 92.1 ${\pm}$ 0.41%. The in vitro drug release profile showed that the initial burst release of DXM from DXM-SLNs was about 68% during the first 2 h, and then the remaining drug was released gradually over the following 48 hours. The biodistribution of DXM-SLNs in mice was significantly different from that of DXM-sol. The concentration of DXM in the lung reached a maximum level at 0.5 h post DXM-SLNs injection. A 17.8-fold larger area under the curve of DXM-SLNs was achieved compared to that of DXM-sol. These results indicate that SLN may be promising lung-targeting drug carrier for lipophilic drugs such as DXM.

Pharmacokinetic difference of berberine between normal and chronic visceral hypersensitivity irritable bowel syndrome rats and its mechanism

Zipeng Gong,Ying Chen,Ruijie Zhang,Qing Yang,Yajie Wang,Yan Guo,Bingbing Zhou,Xiaogang Weng,Xuchen Liu,Yujie Li,Xiaoxin Zhu,Yu Dong 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

Berberine is one of active alkaloids from Rhizomacoptidis in traditional Chinese medicine. The pharmacokineticsof berberine in rat plasma were compared betweennormal and chronic visceral hypersensitivity irritable bowelsyndrome rats (CVH-IBS) established by mechanical colonirritation using angioplasty balloons for 2 weeks after oraladministration of berberine hydrochloride (25 mg/kg) withthe equivalent dose of 22 mg/kg for berberine according tobody weight. Immunohistochemical analysis of c-fos andmyosin light chain kinase (MLCK) and immunofluorescenceanalysis of MLCK in rat colon were conducted. Quantificationof berberine in rat plasma was achieved by using a sensitiveand rapidUPLC-MS/MSmethod. Plasma samples werecollected at 15 different points in time and the pharmacokineticparameters were analyzed by WinNonlin software. Thegreat different pharmacokinetic behavior of berberine wasobserved between normal and CVH-IBS model rats. Compared with normal group, T1/2 and AUC(0–t) of berberinein the model group were significantly increased, respectively(573.21 ± 127.53 vs 948.22 ± 388.57 min; 8,657.19 ±1,562.54 vs 11,415.12 ± 1,670.72 min.ng/ml). Cl/F of berberinein the model group significantly decreased, respectively(13.89 ± 1.69 vs 9.19 ± 2.91 L/h/kg). Additionally,the expressions of c-fos and MLCK in model group werehigher than those in normal group. The pharmacokinetic behaviorof berberine was significantly altered in CVH-IBSpathological conditions, which indicated the dosage modificationof berberine hydrochloride in CVH-IBS were necessary. Especially, improved exposure to berberine in ratplasma inCVH-IBSmodel rats was attributed to increased theexpression of MLCK.

Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro

( Zhe Jin ),( Yan Qing Gong ),( Xin Cheng Qin ),( Jian Zhang ),( Yi Chen Zhu ),( Gui Ting Lin ),( Tom F Lue ),( Zhong Cheng Xin ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSCGFP+) and fibroblasts(FCGFP+) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSCGFP+ and FCGFP+ were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSCGFP+ and FCGFP+ were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSCGFP+ and FCGFP+ and implies a promising technique for tissue engineering.

Molecular and Cellular Microbiology / Biomedical Sciences : Surface-Displayed IL-10 by Recombinant Lactobacillus plantarum Reduces Th1 Responses of RAW264.7 Cells Stimulated with Poly(I:C) or LPS

( Ruo Peng Cai ),( Yan Long Jiang ),( Wei Yang ),( Wen Tao Yang ),( Shao Hua Shi ),( Chun Wei Shi ),( Jing Tao Hu ),( Wei Gu ),( Li Ping Ye ),( Fang Yu Zhou ),( Qing Long Gong ),( Wen Yu Han ),( Guil 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2

Recently, poly-γ-glutamic acid synthetase A (pgsA) has been applied to display exogenous proteins on the surface of Lactobacillus casei or Lactococcus lactis, which results in a surfacedisplayed component of bacteria. However, the ability of carrying genes encoded by plasmids and the expression efficiency of recombinant bacteria can be somewhat affected by the longer gene length of pgsA (1,143 bp); therefore, a truncated gene, pgsA, was generated based on the characteristics of pgsA by computational analysis. Using murine IL-10 as an exogenous gene, recombinant Lactobacillus plantarum was constructed and the capacity of the surface-displayed protein and functional differences between exogenous proteins expressed by these strains were evaluated. Surface expression of IL-10 on both recombinant bacteria with anchorins and the higher expression levels in L. plantarum-pgsA’-IL-10 were confirmed by western blot assay. Most importantly, up-regulation of IL-1β, IL-6, TNF-α, IFN-γ, and the nuclear transcription factor NF-κB p65 in RAW264.7 cells after stimulation with Poly(I:C) or LPS was exacerbated after co-culture with L. plantarum-pgsA. By contrast, IL-10 expressed by these recombinant strains could reduce these factors, and the expression of these factors was associated with recombinant strains that expressed anchorin (especially in L. plantarum-pgsA’-IL-10) and was significantly lower compared with the anchorin-free strains. These findings indicated that exogenous proteins could be successfully displayed on the surface of L. plantarum by pgsA or pgsA’, and the expression of recombinant bacteria with pgsA’ was superior compared with bacteria with pgsA.

Next-generation sequencing of the mitochondrial genome of Dolichovespula panda (Hymenoptera: Vespidae) with a phylogenetic analysis of Vespidae

Xu-Lei Fan,Ya-Jun Gong,Peng-Yan Chen,Qing-Qing Tan,Jiang-Li Tan,Shu-Jun Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

For the first time the mitochondrial genome of a Dolichovespula species, D. panda Archer (Hymenoptera: Vespidae), was sequenced with a next-generation sequencing approach. The sequenced mitochondrial genome is 17137 bp long and consists of 13 protein-coding, 22 tRNA and two rRNA genes, as well as a partial A +T-rich region. Twenty-two of the genes are encoded on the majority strand and 15 genes on the minority strand. All protein-coding genes start with ATN codons and have a TAA termination codon, except for one with a TA codon. Compared with the putative ancestral arrangement of insects, the D. panda mitochondrial genome shows the shuffling of trnN and trnE, and of trnQ and trnM, the translocation of trnY to upstream of trnI, and of trnL1 to the region between trnS2 and nad1 and a reversal of trnS1. A phylogenetic tree within the Vespidae was reconstructed using the 13 protein-coding mitochondrial genes. This shows a sister group relationship between Dolichovespula and a clade formed by Vespa and Vespula. It also corroborated the position of Eumeninae as sister group of the clade Polistinae +Vespinae.