Hybridization에 의한 반수체 재조합 효모균주의 전분 발효능 증진

박선영,김근,이창후 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Hybridization을 통하여 α-amylase와 glucoamylase를 동시에 분비하는 재조합 단수체 효모균주 Saccharomyces diastaticus K114의 전분분해력, 에탄올내성, 당내성, 고온내성등의 발효특성을 증진시키고자 하였다. 이 단수체 효모균주와 glucoamylase의 활성이 좋고 여러가지 발효능이 우수한 단수체 효모균주 S. diastaticus 1177과의 hybridization 결과 얻어진 hybrid HH64주는 에탄올내성, 고온과 당내성이 증진되었으며, 특히 4%의 전분으로부터 1.6%(w/v)의 에탄올을 생산하여, 1.30%(w/v)의 에탄올을 생산한 재조합균주 S. diastaticus K114보다 전분으로부터 에탄올 생성능이 크게 증진되었다. 한편 이 HH64균주의 전분발효에 있어서의 최적온도 및 pH는 각각 30℃와 5이어다. 개발된 hybrid 효모 HH64sms 20%의 전분으로부터 7.5(w/v)의 에탄올을 직접 생산하였다. To improve the fermentation characteristics(such as starch-degradability, ethanol tolerance, sugar and high-temperature tolerance) of recombinant haploid yeast Saccharomyces diastaticus K114, hybridization technique was used. The hybridization partner was S. diastaticus 1177 which had good glucoamylase activity and fermentability. The best hybrid HH64 showed improved ethanol tolerance, sugar and high-temperature tolerance. Especially, the starch-fermentability was significantly improved, since the hybrid produced 1.60% (w/v) ethanol from 4% (w/v) starch, while the recombinant haploid K114 produced 1.30% (w/v) ethanol. The optimum temperature and pH for the starch-fermentation by the hybrid HH64 was 30℃ and 5, respectively. The hybrid yeast HH64 produced 7.5% (w/v) ethanol directly from 20% (w/v) starch.

지진하중을 받는 5경간씩 연속인 10경간 강상자형교의 거더간 충돌 분석

박선준,강성후,이정용 東新大學校 工業技術硏究所 2004 工業技術硏究 Vol.10 No.-

The present study determined whether there is collision between girders due to difference in natural period between neighboring vibration systems when a designed earthquake load is applied to a 3-lane 10-span steel box girder bridge with 5-span continuity, which are equipped with friction pot bearings that receive earthquake load, and examined the stability of a formula that calculates the shift of the sliding bearings, which is used in expansion joint design. In addition, this study developed a simple collision evaluation formula directly applicable in determining the possibility of collision.

Pile Driving에 의한 지반진동 측정 및 진동 영향 평가

박선주,박연수,강성후 東新大學校 工業技術硏究所 1998 工業技術硏究 Vol.4 No.-

In this paper, the ground vibrations have been measured. These were induced during pile driving using vibratory hammer at construction field of the 1st subway line in Gwangju. The measurement of ground vibrations was divided by four step to pile driving processing, auger in reclaimed layer, casing, auger in soft rock layer and H-pile driving. The measured data were analyzed by reliability index to determination the vibration equations and the allowable isolate distance for vibration criteria. These equations and allowable isolate distance were suggested with division of ultimate limit state(β=0), serviceability limit state(β=1.28), safety state(β=3), respectively. These equations can be used to predict the peak vibration values where soil conditions are similar to this test site. From this paper, analysis method for vibrations inference using reliability index was estabilished and the attenuation of the ground vibration induced during pile driving using vibratory hammer(T4W) are suggested.

3경간 연속 중로식 강아치교의 내풍안정성을 고려한 라이즈 비 특성

강성후,박선준,최명기 東新大學校 工業技術硏究所 2004 工業技術硏究 Vol.10 No.-

The most important element is a rise ratio when regarding beauty and economics of arch bridges. Only the effect of dead load has been considered to decide the rise ratio. In this study, when going over the rise ratio of arch bridges, examined the problems, that the determination of the rise ratio by the dead load has, by adding the factor of a determination of optimum rise ratio, which is not only the effect of the dead load that has been currently considered but also the problem with respect to wind resistant dynamic stability that is now taken seriously. Synthetically, when deciding rise ratio that is investigated in basic step of design, it is not necessary to consider the evaluation wind resistant dynamic stability. Therefore the result of this research can be summarized as followes ; If it is identify oneself with rise ratio that the divided ratio due to the tie girder of the 3 continuous spans half-through steel arch bridges cannot cause a big effect in stability the wind resistant dynamic stability. If it is identify oneself with the relative stiffness ratio and girder depth ratio of the 3 continuous spans half-through steel arch bridges that the rise ratio proposes that it uses 0.10~0.30, divided ratio due to tie girder proposes that it uses 1.0~2.5 because of the wind resistant dynamic stability and the stress of the hanger. The rise ratio proposes that it uses 0.16~0.18 is given by the Japanese bridge construction association to obtained in investigation equation between an arch span length and the rise, using 0.1~0.2 at the basic design phase and the divided ratio which using 1.5 due to tie girder generally has been employed to main results and very well agrees with a research accomplishment results.

파괴에너지를 고려한 콘크리트 배합설계 프로그램

강성후,박선준,노호현 東新大學校 工業技術硏究所 2001 工業技術硏究 Vol.7 No.-

In the concrete structure, there are many possibilities existing cracks that are produced and developed from the infinitesimal crack in the member, fatigue or corrosion being not existing in the early period. Also, it is not enough to explain the fracutre behavior using the strength theory. Because the concrete reach to the shear due to main cracking after developing infinitesimal crack when it is received the tension stress. But it is hard to solve the structural problem of concrete, because current analysis and design methods of structure are based on the strength of concrete. Hence the subject of this thesis is programming the design of automatic mix design program to do automatic concrete mix design and to consider fracutre energy depending on mix condition.

강박스 거더교 콘크리트 바닥판에 발생하는 크리이프와 건조수축에 관한 연구

강성후,박선준,김현일 東新大學校 工業技術硏究所 2002 工業技術硏究 Vol.8 No.-

It studies the non-structural crack factors that are produced in Steel Box Girder Bridge concrete floor plate using analytical method. It mainly studies humidity and design standard of concrete strength. It used MIDAS CIVIL Ver 5.4.0, a general structure analysis program that applies drying shrinkage rate of domestic road bridge design standard and standard value of creep coefficient, CEF-FIP standard equation and ACI standard equation from the aspect of creep, drying shrinkage and hydration heat to see the effect of the two factors on concrete crack and found the following result. The analytical results of this study showed that the initial stress, which was obtained by ACI standard, exceeds the allowable tensile stress between 5 to 18 days. This result means that even if a bridge is designed and constructed according to design standard, the bridge can have cracks due to various variables such as drying shrinkage, hydration heat and creep that produce stress in slab.

3경간 중로식 아치교량의 풍하중에 대한 동적안전성

강성후,박선준,정철호 東新大學校 工業技術硏究所 2003 工業技術硏究 Vol.9 No.-

Recently, bridges have been momentous as not only regarding function but also concerning aesthetics. However, when beauty is considered in the design, it is also essential that stability and economics be considered. Moreover, when considering stability, an arch bridge is one of the most stable structures. The most important element is a rise ratio when regarding beauty and economics of arch bridges. Only the effect of dead load has been considered to decide the rise ratio. Therefore, in this study, when going over the rise ratio of 3-Span half through type arch bridges, I examined the problems, that the determination of the rise ratio by the dead load has, by adding the factor of a determination of optimum rise ratio, which is not only the effect of the dead load that has been currently considered but also the problem with respect to wind-resistant dynamic stability that is now taken seriously.

노천 발파패턴에 따른 발파상수의 정량적 평가

강성후,박선준 東新大學校 工業技術硏究所 2002 工業技術硏究 Vol.8 No.-

The actual ground vibrations due to prism cut and trench cut blasting at power plant field have been measured, and the data were analyzed using reliability index(β) to the blast constants and attenuation coefficients. In this study, blast vibrations were suggested with wave component parts of ground vibration to the blast patterns, and were suggested with the division of ultimate limit state(,8 =0), serviceability limit state(β=1.28) and safety state(β =3) respectively. The reliability index 0 means 50% data line obtained by the least squares best-fit line. The reliability index 1.28 and 3 represent bounds below 90%, and 99.9% of the data, respectively. The vibration constants K(particle velocity) for prism cut were evaluated to 333, 619, 1419, respectively and the attenuation coefficients of vibrations was -1.94. From this study, inference and analysis methods of vibration equations using reliability theory were established.

휨부재의 인장철근비 변화에 따른 섬유보강에폭시 판넬의 보강효과평가

강성후,박선준,송영국 東新大學校 工業技術硏究所 2003 工業技術硏究 Vol.9 No.-

FREP is a stiffener that is increasingly used in construction fields today. Concerning it, however, the mechanical characteristics of structures reinforced with it, reinforcement design methods, etc. have not been systematically established yet. The present study evaluated the mechanical characteristics of FREP such as fracture behavior, bending stress enhancement, etc. through a bending test using as variables steel ratio of RC beams reinforced with FREP and the thickness of FREP reinforcement. The RC Beams used in the test showed two types of fraction, which were bending tension fraction and edge stripping fracture, and when the ratio of steel ratio (ρ) to FREP ratio (ρ_(F)) is larger than 1.8, it showed edge stripping fracture. Under same reinforcement condition, if steel ratio is relatively low, reinforcement effect appeared to be high. In addition, if reinforcement thickness increases, the flexural rigidity rises and, as a result, the maximum load also increases, but an excessive increase of reinforcement thickness makes stress concentrate on the reinforced end and cause unexpected brittle fracture (edge stripping fracture) even at a light load.

한 중소도시의 청소년 약물사용 실태조사

이후경,김선재,윤성철,봉수연,안현주,박선영 대한신경정신의학회 2001 신경정신의학 Vol.40 No.1

-초록- 연구목적: 약물사용의 가능성이 가장 높은 시기는 십대 후반에서 이십대 초반의 청소년기로 알려져 있다. 또한 청소년기의 약뮬사용은 빠른 의존의 가능성, 심각한 약물남용으로 진행할 가능성, 약물로 인한 공격성의 증가 등으로 심각한 사회문제가 될 여지가 많다. 그러므로 저자들은 중소도시에서 청소년 약물사용의 예방 및 치료를 위한 정책을 마련하는데 기초가 될 수 있는 청소년 약물사용 실태를 조사하는 것을 목적으로혹시청소년상담실의 기금을 받아 본 연구를 시행하였다. 저자들이 세운 연구가 설은 다음과 같다. 첫째, 나이, 성(性)과 같은 개인적 요인에 따라 청소년의 약물사용에 차이가 있을 것이다. 둘째, 가정, 또래집단과 같은 환경적 요인에 따라 청소년의 약물사용에 차이가 있을 것이다. 셋째, 음주, 흡연, 비행과 약물사용과는 상화관련이 있을 것이다. 방법: ㅎ시 4개 중학교 학생 833명(51.6%), 3개 고등학교 학생 780명(48.4%), 성별은 남자(49.5%), 여자(50.5%), 총 1613명을 대상으로 하여 1999년 7월 12일부터 14일까지 3일간 설문조사를 실시하였다. 결과: 1) 술(59.4%), 담배(34.4%)의 경험이 가장 높았고, 의약품 중에는 진통제(33.1%), 흡입제(inhalants)중에는 본드(0.7%), 마약류 중에서는 히로뽕(0.4%)의 사용이 가장 많았다. 2) 술, 담배, 수면제, 각성제, 신경안정제의 사용경험이 중학생 보다 고등학생이 거의 두 배 이상 높았다. 3) 약물을 사용하지 않는 학생의 부모 화목도 점수가 약물을 사용하는 학생의 부모 화목도 점수보다 의미 있게 높은 것으로 나타났다. 4) 흡연 및 음주경험이 있는 학생의 친구들이 그렇지 않은 학생의 친구들에 비해 술, 의약품, 흡입제를더 많이 사용하였다. 5) 술, 담배, 약물을 사용하는 학생들이 비행 가능성이 높았다. 6) 술을 사용하게 되는 가장 많은 이유는 '친구들과 어울리기 위하여'(55.0%), 담배를 사용하게 되는가장 많은 이유는 '심심해서'(50.9%)이었다. 7) 술, 담배, 약물을 사용하면 다른 것을 사용할 가능성이 높은 것으로 나타났다. 결론: 한국의 청소년 약물사용경험은 이제까지의 연구와 본 연구를 종합하여 볼 때 지속적으로 증가하고 있다. 향후 약물사용을 줄이기 위해서는 국가적인 차원에서 약물사용 실태조사가 선행되어야 하고, 그 결과에 따른 적절한 대책의 수립과 정신과 의사들의 적극적인 참여가 필요하다. Objectives : The purpose of this research is to help solve adolescent issues involving substance uses, which may become begin to undergo many changes(i.e.practising various roles, thereby leading to independent life styles). More often than not, adolescent drug problems develop into social problems, chiefly behavioral imitations and heavy dependence on medicine. The authors conducted a survey of adolescent substance uses to develop preventive measures against the drug habit prevalent among young people, thereby formulating a policy to deal with problematic community. Funded by a Yuth Counselling Center in the city of H. the research was conducted on the following assumptions that there will be : Firstly, a comparison of drug uses by sex and age ; Secondly, variance of substance users by family background and peer group ; Thirdly, a combination of heavy drinking, smoking and delinquency resulting in the drug habit. Methods : the survey included a total of 1613 students during 3 days, 12 through 14, July, 1999. The subjects were 833 pupils(51.6$) from 4 middle schools and 780 students(48.4%) from 3 high schools in the city of H with a ratio of 49.5%(males) and 50.5%(females). Results : 1) Experience of substance use is most referable to drinking(59.4%) and smoking(34.4%) while given to analgesics(33.1%) among medicines, glue(0.7%) among inhalants and philophone(0.4%) among narcotics. 2) High school students found more than double the amount of smoking and drinking as well as uses of hypnotics, stimulants and sedatives by junior high school students. 3) Our resalt showed was significantly noted that non-users had a better relationship with their parents than most users. 4) Smoking and drinking problean could be more easily developing into adolescent substance uses. 5) Alcohol, cigarette, and drug users showed more misconducts than other adolescents. 6) Most adolescents believe theat drinking is necessary for making friends(55.0%), while smo-king is just for killing time(50.9%) 7) Drinking, smoking, and other substance users were found to develop into more proble-matic habits. Conclusion : The present survey and several others show that adolescent substance uses in Korea are on the rise. Therefore, extensive counter-measures will be necessary followed by nationwide epidemiological survey of the pending issue, with the psychiatrists participating in the campaign.