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Methallothionein Gene과 이배체를 이용한 외래 알파아밀라제의 효모에서의 생산 증진
김근 수원대학교 기능성생명소재연구소 2002 자연과학연구논문집 Vol.1 No.-
In order to increase the production rate of heterologous mouse salivary α-amylase from yeast cell, gene amplification using methallothionein gene and improvement of plasmid-stability using diploid cell were conducted. A DNA fragment harbouring copper ion resistant gene(CUP1) was inserted into the vector containing the α-amylase gene to be pMS12(CUP1). The transformant containing pMS12(CUP1) showed 30% more α-amylase activity in the presence of 0.1 mM CuSO₄in the culture medium, suggesting that the copy number of the vector containing the CUP1 in the yeast cell was increased by the exogenous copper ion and consequently the α-amylase gene on the same vector was amplified together with the vector. The mitotic stability of the vector containing α-amylase gene was 16% higher in diploid cells after 50 generations of cell multiplication than that in haploid cells.
식도 자연파열[Boerhaave 증후군]의 외과적 치료;2례 보고
김근,장봉현,이종태,김규태,Kim, Keun,Chang, Bong-Hyun,Lee, Jong-Tae,Kim, Kyu-Tae 대한흉부심장혈관외과학회 1992 Journal of Chest Surgery (J Chest Surg) Vol.25 No.8
The spontaneous rupture of the esophagus is an uncommon disease, but without early diagnosis and treatment, the mortality rate is high. This report is on the two cases treated at the Department of Thoracic and Cardiovascular Surgery, College of Medicine, Kyu-ngpook National University. The first patient, after heavy drinking, had vomiting followed by chest pain, dyspnea and subcutaneous emphysema. After diagnosis using an esophagogram, an operation was perfomed. About a 4cm rupture was found at the lower left part of the esophagus and was primarily sutured with the intercostal muscle. The patient was weaned from the ventilator after 40 hours. The second patient had symptoms the same as the first case. Six days after the app-earence of the symptoms, the patient was treated by the Thais onlay gastric patch method. The leakage happened after the surgery and he received a conservative treatment. After discharge no abnormalities, such as leakage and stricture, were found on the eso-phagogram The two patients now live a normal life.
김근,장봉현,이종태,김규태,이상범,Kim, Keun,Chang, Bong-Hyun,Lee, Jong-Tae,Kim, Kyu-Tae,Lee, Sang-Bum 대한흉부심장혈관외과학회 1992 Journal of Chest Surgery (J Chest Surg) Vol.25 No.3
We evaluated the correlationship between the predicted defect size at preoperative examination and the actual defect size at operation room, by examining 69 cases of ventricular septal defect operated at the deparment of Thoracic and Cardiovascular surgery, Kyungpook University Hospital from January 1988 to December 1990. We excluded cases associated with other cardiac anomalies. Of the 69 cases, 39 are male and 30 female, forming 1.3:1 sex ratio in males favor. Their age range from 6 months to 16 year, and 4.3 on the average Their body weights are from 6 to 45kg and 15 on the average. According to Soto`s classification, perimembranous type costitutes 42 cases[61%], doubly committed subarterial type 23 cases[33%], and muscular type 4 cases[6%]. The average diameter of defect size is 8.0$\pm$3.5mm measured in 2D-echocardiogram, 5.6$\pm$3.4mm in angiogram, and 7.4$\pm$4.4mm in operative field. There is statistically significant correlation between the size from 2D-echocardiogram and actual defect size[p=0.001], and no significant difference between the two. Especially in the cases without anurysmal formation, they are nearly the same. Cardiothoracic ratio, pulmonary to systemic flow ratio, pressure ratio and resistance ratio also have statistically significant correlation. Main pumonary artery to descending aorta diameter ratio is correlated with the actual defect size. There is statistically significant correlation between the size from angiogram and actual defect size with some difference.
활로씨 4징증을 동반한 완전방실중격결손의 수술치험;1례 보고
김근,장봉현,이종태,김규태,Kim, Keun,Chang, Bong-Hyun,Lee, Jong-Tae,Kim, Kyu-Tae 대한흉부심장혈관외과학회 1992 Journal of Chest Surgery (J Chest Surg) Vol.25 No.8
A 25-month-old patient with complete atrioventricular septal defect and Tetralogy of Fallot underwent repair of both anomalies. The diagnosis was established preoperatively by 2D-echocardiography, cardiac catheterization and cardioangiogram, Repair was accomplished using cardiopulmonary bypass and profound hypothermia to 18C, Closing of the atrioventricular septal defect was achieved with the use of two Dacron patchs by an atrial approach alone. Infundibulectomy and outflow tract reconstruction with the transannular pericadial patch containing a monocusp were performed. Upon the postoperative evaluation by 2D-echocardiography, mitral regurgitation was absent, but a tiny dehiscence of ventricular patch and minimal tricuspid regurgitation were noticed.
김근,장봉현,이종태,김규태,Kim, Keun,Chang, Bong-Hyun,Lee, Jong-Tae,Kim, Kyu-Tae 대한흉부심장혈관외과학회 1993 Journal of Chest Surgery (J Chest Surg) Vol.26 No.5
We analyzed the patients who were admitted to Surgical Intensive Care Unit[SICU] of Kyungpook National University Hospital from January 1987 through December 1991. The results were as followings: The total number of patients for 5 years was 2446 and 1553[63.5%] were male,893[36.5%] were female. The average age was 34.1 years old and the greatest age group was the 1-9 age group.The number of the patients in the Department of Thoracic and Cardiovascular Surgery, which was the highest among all departments,was 1608 [65.7%].Congenital Heart disease was the most common among all diseases.The patients who had undergone any kinds of operations were 89.9% of all patients.The averge stay in SICU was 5.2 days.The overall mortality rate was 12.1% ,which included hopelessly discharged patients and the highest mortality rate occurred in the patients over 80 years old.The highest cause of death was cardiac problem[30.4%]. The next was sepsis.The proportion of patients who had received a mechanical ventilatory support was 35.7% and 52% of those patients belonged to the department of thoracic and cardiovascular surgery.
일차성 자연기흉에서 2개의 삽입구를 이용한 흉강경 폐기포절제술
김근,Kim, Keun 대한흉부심장혈관외과학회 2001 Journal of Chest Surgery (J Chest Surg) Vol.34 No.8
비디오흉강경을 이용한 일차성 기흉의 수술은 흉강경, 내시경용감자 그리고 자동봉합기를 위한 3개의 삽입구를 필요로 하였다. 그러나 기흉의 경우에 따라서는 내시경루프를 잘 이용할 경우 2개의 삽입구만으로도 수술이 가능하여 술후 통증 및 흉터의 최소화에도 이바지 할 수 있을 것이다. Usually, Bullectomy with VATS requires three ports on a chest wall for thoracosope, endo lung grasper, and endo auto-suture. However, in some case, the author could successfully accomplish operation using the endo-loop through two ports on a chest wall. The technique reduces postoperative pain and decreases operative wound.
외래 α-amylase 유전자의 효모 엽색체내로의 도입
金槿 水原大學校 1991 論文集 Vol.9 No.-
To improve the mitotic stability of heterologous mouse salivary α-amylase cDNA gene with a yeast promoter in a yeast cell, integrating plasmid vectors containing the α-amylase gene were constructed and introduced into the yeast cell. The results showed that the linearized type of the vector was superior in the transformation effciency and the rate of the expression of the α-amylase gene than the circular type of the vector. The yeast transformant having a linearized plasmid vector exhibited higher value in mitotic stability than that of the yeast transformant having episomal plasmid vector.
세포벽 분해효소에 의한 여러 종류의 효모들의 원형질체 형성의 최적 조건
김근 수원대학교 기초과학연구소 1997 基礎科學論文集 Vol.6 No.-
The optimum conditions to prepare the protoplasts of various yeasts belonging to different genus and species by using lyticase of Arthrobacter luteus as a cell-wall digesting enzyme. For protoplast formation of Saccharomyces uvarum and Kluyveromyces marxianus by liticase, 1.2 M KCl of osmotic stabilizer, 200 unit/ml of lyticase concentration and 90 min of reaction time, 10-20 mM/ml of EDTA and 4%(v/v) mercaptoethanol as the pretreatment agents were the optimum conditions, and optimum pHs for S.uvarum and K. marxianus were 7 and 8, respectively. The optimum lyticase concentration and incubation time for cell-wall digestion was 200 unit/ml and 60 min for S. diastaticus and Brettanomyces custerssi, respectively. The maximum rate of protoplast formation was 96% for S. uvarum, 99% for K.marxianus, 72.5% for S. diastaticus, and 56% for B. custerssi.