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Differential Expression Patterns of Gangliosides in Micro-pig solid organs
So-Dam Lee,Dong-Hoon Kwak,Kyu-Tae Chang,Ji-Su Kim,Jae-Sung Ryu,Jin-Hyoung Cho,So-Hyun Lee,Ju-Taek Lee,Da-hyun Park,Seul-Yi Lee,Young-Choon Lee,Young-kug Choo 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Ganglioside are ubiquitous membrane component in mammalian cells and suggested to play important roles in various cell functions such as cell-cell recognition, differentiation and transmembrane signaling. These compounds are localized in a glycosphingolipid-enriched microdomain on the cell surface and regulated by the glycosphingolipid composition. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, differential expression patterens of gangliosides of Micro-Pig solid organs, which are heart, kidney, liver, pancreas and spleen. We examined expression level of high-performance thin-layer chromatography (HPTLC) showed that all of solid organs contained GM3, GM2, GM1 and GD1a as major gangliosides. Especially, ganglioside GD1b expressed in liver and pancreas, not in heart, kidney and spleen. Also, GT1b expressed in liver, kidney and spleen, not in heart and pancreas. As a results suggest that differential expression patterens of gangliosides of Micro-Pig solid organs are related immune rejection response in xenotransplantation by biomarker.
So-Hyun Lee,Jae-Sung Ryu,Jin-Hyoung Cho,So-Dam Lee,Ju-Taek Lee,Da-Hyun Park,Seul-Yi Lee,Dong Hoon Kwak,Young-Kug Choo 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoetic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast- differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.
( Se Yeon Ahn ),( So Dam Yi ),( Won Jong Seo ),( Myeong Jung Lee ),( Young Keun Song ),( Seung Yong Baek ),( Jin Ha Yu ),( Soo Hyun Hong ),( Jin Young Lee ),( Dong Wook Shin ),( Lak Shin Jeong ),( Min 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.3
Endocannabinoids can affect multiple cellular targets, such as cannabinoid (CB) receptors, transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and peroxisome proliferator-activated receptor g (PPARg). The stimuli to induce adipocyte differentiation in hBM-MSCs increase the gene transcription of the CB1 receptor, TRPV1 and PPARg. In this study, the effects of three endocannabinoids, N-arachidonoyl ethanolamine (AEA), N-arachidonoyl dopamine (NADA) and 2-arachidonoyl glycerol (2-AG), on adipogenesis in hBM-MSCs were evaluated. The adipocyte differentiation was promoted by AEA whereas inhibited by NADA. No change was observed by the treatment of non-cytotoxic concentrations of 2-AG. The difference between AEA and NADA in the regulation of adipogenesis is associated with their effects on PPARg transactivation. AEA can directly activate PPARg. The effect of AEA on PPARg in hBM-MSCs may prevail over that on the CB1 receptor mediated signal transduction, giving rise to the AEA-induced promotion of adipogenesis. In contrast, NADA had no effect on the PPARg activity in the PPARg transactivation assay. The inhibitory effect of NADA on adipogenesis in hBM-MSCs was reversed not by capsazepine, a TRPV1 antagonist, but by rimonabant, a CB1 antagonist/inverse agonist. Rimonabant by itself promoted adipogenesis in hBM-MSCs, which may be interpreted as the result of the inverse agonism of the CB1 receptor. This result suggests that the constantly active CB1 receptor may contribute to suppress the adipocyte differentiation of hBM-MSCs. Therefore, the selective CB1 agonists that are unable to affect cellular PPARg activity inhibit adipogenesis in hBM-MSCs.
Ju-Taek Lee,Jae-Sung Ryu,Dong Hoon Kwak,Jin-Hyoung Cho,So-Dam Lee,Da-Hyun Park,Seul-Yi Lee,Yun Jum Park,Seong Sun Kang,Pyoung Jun Kim,Young-kug Choo 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Gangliosides are complex glycosphingolipids that contain one or more sialic acids, the major components of cytoplasmic cell membranes. these are tought to play a role in the control of biological processes including cell surface interaction, cell differentiation and transmembrane signaling. Particularly, ganglioside GM3 is abundant in the kidney tissue. Diabetes mellitus is one of the most serious metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both, associated with long-term damage, dysfunction, and failure of various organs, especially the eyes, kidneys, nerves, heart, and blood vessel. In this study, we demonstrated that gangliosides expression patterns on kidney of mulberry-treated to STZ-induced diabetic rats. High-performance thin-layer chromatography analysis and immunostating showed that ganglioside GM3, GM2, GM1, GD1a, GD1b and GT1b were expressed in normal rats kidney, whereas ganglioside expression were strongly reduced in the kidney of diabetic rats. In 1.0% mulberry administration to diabetic rats kidney, ganglioside GM3, GM2 and GM1 expression were not different compared to normal rats kidney, whereas the other bands were not seen. Therefore, alteration of ganglioside expression in kidney of STZ-induced diabetic rats seem to be importantly involved in the development of glomerular hypertrophy and proteinuria.
Jae-Sung Ryu,Dong Hoon Kwak,Jeong-Woong Lee,Jin-Hyoung Cho,So-Dam Lee,Ju-Taek Lee,Da-Hyun Park,Seul-Yi Lee,Young-kug Choo 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Gangliosides are complex glycosphingolipids that contain one or more sialic acids, the major components of cytoplasmic cell membranes. these are tought to play a role in the control of biological processes including cell surface interaction, cell differentiation and transmembrane signaling. Direct reprogramming of somatic cells into induced pluripotent stem (iPS) cells can be achieved by overexpression of Oct-4, Sox-2, Klf-4 and c-Myc transcription factors, but only a minority of donor somatic cells can be reprogrammed to pluripotency. In this study, we demonstrated that differential expression patterns of gangliosides in iPS cells. First, we investigated characterization of iPS using Alkaline phosphate (AP) and stage-specific embryonic antigen-1 (SSEA-1) straing and RT-PCR. The iPS cells expressed AP and SSEA-1. In RT-PCR analysis, iPS cells also expressed Oct-4, Sox-2 and Nanog mRNA. Immunocytochemistry and high-performance thin-layer chromatography analysis showed that mouse embryonic fibroblast (mEF) expressed GM3, GM2 and GD3, but not GM2 in iPS cells. Furthermore, ganglsiode expression pattern were equal to mouse embryonic stem cell and iPS. In RT-PCR analysis confirmed that GM3 and GD3 synthase mRNA expressed in iPS, whereas mEF also expressed GM2 synthase mRNA. These results suggest that ganglioside GM2 play a important role in regulating the reprogramming of somatic cells into iPS.
Jin-Hyoung Cho,Dong-Hoon Kwak,Kyu-Tae Chang,Ji-Su Kim,Jae-Sung Ryu,So-Dam Lee,So-Hyun Lee,Ju-Taek Lee,Da-Hyun Park,Seul-Yi Lee,Young-Choon Lee,Young-kug Choo 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1
Ganglioside are ubiquitous membrane component in mammalian cells and suggested to play important roles in various cell functions such as cell-cell recognition, differentiation and transmembrane signaling. These compounds are localized in a glycosphingolipid-enriched microdomain on the cell surface and regulated by the glycosphingolipid composition. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, differential expression patterens of gangliosides between human vein endothelial cells (ECV304) and micro-pig aortic endothelial cells (MPAECs, primary cultured) was investigated. we examined expression level of high-performance thin-layer chromatography (HPTLC) showed that ECV304 cells contained GM3 and GM2 as major gangliosides. However, MPAECs contained only GM3 as major ganglioside. Furthermore, we confirmed that human serum induces cell death of MPAECs. As a results suggest that differential expression patterens of gangliosides of ECV304, MPAECs are related immune rejection response and cell death with xenogeneic serum in xenotransplantation by biomarker. This study was supported by a grant from the Korean Rural Development Administration Agenda Program, 2010030106112200103) and a research grant from the Ministry of Education, Science and Technology (KGC5401011), Republic of Korea.