위장관의 원발성 B 세포 악성 림프종의 재분류와 bcl-2, cyclin D1, bcl-6 및 p53 발현의 의미

서정균,김태원,김병수,조은택,박찬국,김만우,이미자,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : In recent years, the REAL (Revised European American Lymphoma) classification provided a new category of MALT (mucosa-associated lymphoid tissue) lymphoma and mantle cell lymphoma in B cell lymphomas. We have analyzed the expression of bcl-2, cyclin D1, bcl-6 and p53 and correlated with the subtypes and histologic grade of primary gastrointestinal B cell lymphoma. Also we investigated the usefulness of immunophenotypic features in diagnosis of low grade B cell lymphoma. Materials and Methods : Twenty-two cases of primary gastrointestinal B cell lymphoma were recategorized in low grade MALT lymphoma, low/high grade MALT lymphoma, high grade MALT lymphoma, and diffuse large cell lymphoma according to the morphological findings. We investigated the expression of bcl-2, cyclin D1, bcl-6 and p53 by immunohistochemical method. Results : The bcl-2 protein expression was higher in the low grade MALT lymphoma than in the high grade lymphoma. The cyclin D1 protein expression was negative in all cases. The expression of bcl-6 and p53 protein was negative in all low grade MALT lymphoma. Conclusion : The results suggest that we can differentiate the low grade lymphoma from the high grade lymphoma by immunohistochemical staining for bcl-2, bcl-6, and p53 protien.

시설농업지에서의 해충과 천적의 발생상

서미자,안수정,박덕기,윤영남 충남대학교 농업과학연구소 1997 농업과학연구 Vol.24 No.1

For the investigation of occurrence of insect pests and their natural enemies, total number of 353 controlled agricultural farms were visited and surveyed. In the 73% of total controlled agricultural farms, 52 species insect pests were occurred. Among these pests, the western flower thrip (Frankliniella occidentalis), the two spotted spider mite (Tetranychus urticae), the greenhouse whitefly (Trialeurodes vaporariorum), the cotton aphid (Aphis gossypii) and the green peach aphid (Myzus persicae) were mainly occurred. On the other hand, small numbers of natural enemies were sustained in 40 controlled agricultural farms, which was 11% and 17% of visited farms and pest occurred farms, respectively. For the most part of collected natural enemies, there were included in Braconid, Coccinellid and Anthocorid, as 63, 20 and 8% of them, respectively.

주사전자현미경을 이용한 온실가루이(Trialeurodes vaporarium) 성충 부속지의 외부 미세구조 관찰

서미자,김기덕,김남성,박수진,채순용,윤영남 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

온실가루이의 외부형태적 특징을 주사전자현미경 사진을 통해 관찰하였다. 우선 이들은 포탄모양의 알을 산란하는데 짧은 갈고리모양의 구조에 의해 잎 뒷면에 붙어있는 것이 확인되었다. 촉각의 길이는 0.3㎜로 전체가 6마디로 구성되어 있고, 첫마디는 굵고 짧으며 둘째 마디부터 가늘고 긴 채찍모양을 하고 있다. 끝마디인 6번째 마디의 끝에는 길이가 10㎛정도의 가늘고 비교적 긴 감각기를 가지고 있어 기주를 탐색하는 과정에서 식물체의 표면을 탐지하는데 사용하는 것으로 사료되며, 바로 밑부분에는 길이가 7-8㎛정도의 비교적 굵은 감각기 2개가 존재하고 있는 것으로 확인되었다. 이 감각기는 반대편에는 존재하고 있지 않은 비대칭 분포를 보이고 있으며, 또한 두개의 모양이 서로 상이함으로 볼 수 있다. 이들의 기능은 주로 기주식물을 탐색하거나 후각기능의 화학적 감각기의 역할을 하는 것으로 추정할 수 있다. 섭식행동이 이루어지는 온실가루이 구기는 전형적인 piercing-sucking type으로, 구침이 길게 잘 발달되어 있다. 구기의 끝부분은 10여개의 미세감각기가 분포고 있어 식물체 표면을 탐색하고 맛을 보는 미각기능을 하고 있으리라 추측할수 있다. 구침은 종종 구기에서 분리되기도 하지만, 구침을 잡아주는 지지대가 기부쪽에 자리하고 있음을 알 수 있다. 구침은 두 개의 반원형이 붙어 있는 형상을 하고 있으며, 다른 흡즙형 구침과는 달리 톱날모양의 구조를 볼 수 없었다. 한편 온실가루이의 다리 끝에 붙어 있는 발톱은 식물체에 고정시키기 위한 구조로 보이는 두 개의 갈고리 형태의 발톱과 그 사이에 중앙지지대 형태의 구조인 paranychium을 포함하여 3갈래의 구조를 하고 있다. External morphology characteristics of the greenhouse whitefly, Trialeurodes vaporarium were investigated by scanning electron microscopy. The antennae of the greenhouse whitefly was 6 segments, rod-shape, and 0.3mm length. On the 6th segment, there were many sensilla for searching host-plant as olfactory receptor. The mouthpart of the greenhouse whitefly was a piercing-sucing type, then its stylet was well developed for piercing plant leaf tissue. Claw of the foreleg was a 3-war hook shape including paranychium for attaching plant surface to pierce and lay egg.

Cellulose 첨가 식이가 고콜레스테롤 식이를 섭취한 암컷 쥐의 혈중 지질 농도에 미치는 영향

서지나,최미자 啓明大學校 生活科學硏究所 2006 科學論集 Vol.32 No.-

Numerous studies have been done on the effect of fiber on cholesterol metabolism. Most of studies reported a hypocholesterolemic action of fiber in rats fed a high cholesterol diet. However, a few studies reported that addition of cellulose to the diet increased the serum level of cholesterol in rats. Therefore, more studies are needed to figure out the effects of cellulose on cholesterol metabolism. The purpose of this study was to investigate the effect of dietary cellulose supplementation on plasma lipid concentrations in rats fed a high cholesterol diet. Twenty female rats (body weight 190 ± 2.9 g) were randomly divided into two groups. The rats in the control group were fed on 5% cellulose diet (control) and those in the experimental group were fed with 10% cellulose diet. All rats were fed with the experimental diets and deionized water ad libitum for 5 weeks. The plasma lipid concentrations were measured using commercial kits with enzymatic methods. There were no significant differences in the body weight gain, food intake and food efficiency ratio between the control and experimental groups. The rats fed cellulose supplemented diet had significantly higher plasma total cholesterol concentration than those fed control diet. The plasma concentrations of triglyceride were slightly reduced in the rats fed cellulose supplemented diet compared to those fed control diet. But plasma triglyceride concentrations were not significantly different between two groups. More detailed research is necessary to explain why the effectiveness of cellulose in reducing the plasma cholesterol concentration in high cholesterol fed rats has been different.

생물적 방제 인자로서의 무당벌레(Harmonia axyridis) : 하우스에서 계절에 따른 진딧물 방제효과 Control Effects of Aphid Populations in the Greenhouses at Different Seasons

서미자,윤영남 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.1

무당벌레(Harmonia axyridis)를 시설재배지대에 발생하는 진딧물류 방제에 적용하기 위해서 농가포장에서 무당벌레를 이용하여 진딧물 수종에 대한 방제효과를 관찰하였다. 겨울과 봄. 여름철에 무우테두리진딧물과 복숭아흑진딧물, 목화진딧물이 발생한 겨자, 신선초, 케일, 가지를 재배하고 있는 시설재배기에 무당벌레 성충과 유충을 방사하여 진딧물의 방제효과를 조사하였다. 실험에 사용된 무당벌레는 월동개체군을 채집하여 실내에서 유지 보관한 것과 이들 월동개체군으로부터 진딧물을 공급하여 증식시킨 성충과 유충을 사용하였다. 겨울철에 수막시설을 갖추고 있는 시설재배지에 발생하는 진딧물을 효과적으로 방제하기 위해서는 다른 계절에 비해서 많은 양의 무당벌레의 성충과 유충을 투입하여 진딧물 개체군밀도를 낮출 수 있었다. 봄철과 여름철에는 진딧물의 종류와 밀도에 다라서 무당벌레의 방사밀도를 달리하여 원하는 시기에 효과적으로 진딧물 개체군 수준을 낮출 수가 있었다. 무당벌레를 투입하는데는 시설재배지 내의 온도와 습도 조건이 중요한 환경요인으로 작용하며, 진딧물 개체군의 증가율과 진딧물 초기밀도, 투입하는 무당벌레의 발육단계 등의 요인들을 고려하여야 할 것으로 판단된다. Application of the Asian ladybird (Harmonia axyridis) to control several species of aphids in the plastic green houses in mind, control effects of aphid populations regulated by the Asian ladybird were observed. The green peach aphid, the turnip aphid. and the cotton aphid were present on mustard plants, Angelia uths, ornamental kales, and egg plants at greenhouses in spring, summer, and winter. Adults and larvae of the Asian ladybird used in experiments were collected from aggregated sites at Taejon in the autumn and reared on the cotton aphid in the laboratory. In winter. more number of adults and larvae of ladybirds than in other seasons were needed to control aphid population in successively double plastic greenhouses with supplied subterranean water for keeping warmth. In spring and summer. it was possible to keep the aphid populations low when necessary by manipulating ladybird populations according to the density of aphids. On the other hand, the innate increasing rate of aphid, the aphid population density at the time of applying ladybird. the predacious ability of ladybird at specific developmental stages, and needed periods should be taken into account to control aphids. In addition, the environmental factors, for example, optimum temperature and humidity should be considered to be biologically effective when ladybirds are released to greenhouses.

온도 기울기(temperature gradient) 젤에서 Heteroduplex Analysis 기법을 이용한 돌연변이 DNA의 검출

조용석,구미자,박귀근,박영서,강종백 A.R. Liss 1998 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.18 No.2

일부 고지혈증 폐경 여성의 Isoflavone 공급에 따른 혈중지질 변화에 관한 연구

이다홍,승정자,이행신,김미현,서유리 대한지역사회영양학회 2001 대한지역사회영양학회지 Vol.6 No.1

Intake of soy protein may decrease the risk factors associated with cardiovascular disease in postmenopausal women. This study was designed to investigate the effects of isoflavone supplementation on serum lipids in 16 hyperlipidemic postmenopausal women. For this purpose, an intervention study was conducted for 12 weeks. Subjects were healthy, free-living women consuming habitual diets with 0.3 g/d of isoflavone. Food and nutrient intake was obtained by 24-hr recall method and anthropometric measurements were made. Systolic and diastolic blood pressure, total serum cholesterol, HDL-cholesterol and LDL-cholesterol were determined before and after the isoflavone supplementation. The results were summarized as follows. The average age, height, weight and BMI of the subjects were 65.3 years, 151.4 cm, 62.2 kg and 27.1, respectively. The systolic blood pressure and diastolic blood pressure were not reduced significantly with isoflavone supplementation. Total cholesterol (p < 0.001), HDL-C (p < 0.05), and LDL-C (p < 0.01) were significantly increased after isoflavone concentration. In conclusion, isoflavone supplementation was not effective to modify risk factors for cardiovascular disease.

꽃노랑총채벌레(Frankliniella occidentalis)의 외부 미세구조의 특징

김기덕,김남성,박수진,서미자,채순용,윤영남 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

SEM을 사용하여 꽃노랑총채벌레, Franklimella occidentalis의 외부형태적 특징을 살펴보았다. 꽃노랑총채벌레의 촉각은 모두 8마디로 구성되어 있으며 길이는 0.25㎜정도이다 또한 3번째 마디와 4번째 마디는 부메랑 모양을 하고 있으며 5번째와 6번째 마디에는 길이가 7㎛정도의 감각기가 있다. 8번째마디에는 8개의 감각기가 있다. 입틀은 비교적 앞으로 튀어나온 모양을 하고 있으며 많은 감각기가 분포하고 있는데 모두 14개의 감각기가 양쪽으로 배열되어 있다. 다리의 구조는 발톱을 가지고 있지 않다. The external morphology of Frankliniella occidentalis was studied by using the scanning electron microscopy (SEM). The antenna was consisted of 8 segments and the length of antenna was 0.25mm. The 3rd segment and 4th segment were bent. The 5th segment and 6th segment had sensilla with 7㎛ long. The 8th segment had 8 sensilla. The mouthpart was protruded and distributed many sensilla on there. The leg had no claw.

주사전자현미경을 이용한 토마토에 발생하는 아메리카잎굴파리(Liriomyza trifolii)의 외부형태적 특징

윤영남,김기덕,박수진,김남성,서미자,채순용 충남대학교 농업과학연구소 2000 농업과학연구 Vol.27 No.1

SEM을 이용하여 아메리카잎굴파리(Liriomyza trifolii)의 암 ·수컷 성충의 외부형태적 특징을 살펴보았다. 더듬이에 있어 감각모의 3가지 형태는 더듬이가는마디(funicle)에서 관찰되었으며, 모상감각기(trlchold sensillum)는 말단 부위가 뽀족한 특징적인 형태를 하고 있고 감각기들 중에서 가장 많이 분포하고 있다. 원추상 감각기(basiconic sensillum)는 길이가 13㎛, 지름이 1.4㎛에서 1.2㎛인 원형곡선형이다. 홈형 감각기(grooved sensillum)는 간상(peg) 끝에서 나온 손 모양을 하고 있으며, 이 모든 감각기들은 후각과 연관되어져 있는것으로 추정된다. 구기는 홉즙형으로 작고 펼쳐진 윗입술(labrum)과 합쳐져서 하나의 연결된 주둥이(rostrum)을 형성하고 있다. 가느다란 한 쌍의 작은턱수염(maxillary palp)은 긴 관 모양으로 각각의 세로로 이어져 있다. 발마디는 5마디로 이루어져 있으며 그 한가운데에는 패드모양의 흡착반(pulvillus)과 가시모양의 발톱사이돌기(empodium)가 있다. 아메리카잎굴파리는 흡착반과 발톱사이돌기에서 미끄러운 표면에 부착할 수 있는 물질을 분비한다. 수컷의 생식기는 매우 복잡하며 삽입구(aedeagus)는 생식기관으로서 가장 일반적인모양이다. 반면에 암컷의 생식기는 상대적으로 간단한데 이 구조는 외부로 돌출된 관모양이었다. 또한 이러한 결과를 이미 발표된 다른 여러 종의파리와 비교하였다. The external morphology of male and female of Liriomyza trifolii (Burgess) was studied using the scanning electron microscopy (SEM). Three types of sensilla were identified on the funicle of antennae. The trichoid sensilla were most conspicious and distributed with a characteristic sharp structure at the apical end. Basiconic sensilla were showed distal curvature about 13㎛ long and 1.2㎛ to 1.4㎛ in diameter. Grooved sensilla were showed a finger-like projections at the peg apex. The function of these sensilla likely to be olfactory. The mouthparts were adapted for sucking food, and formed a elongate rostrum which was incorporated with labrum. The labrum was a small and flap-like shape. The slender and paired maxillary palp seemed to combine with each other lengthwise to long tube. The tarsus typically consisted of 5 segments, which might be variously modified, principally by thickening or flattening. The segment healed a pair of claws. Beneath the claws, there was a pair of pad-like pulvillia and a bristle-like median empodium. L. trifolii secreted on the pulvillus and empodium an adhesive substance that enable them to cling to smooth surfaces. Male terminalia was complicated. The name 'aedeagus' seemed the best available general term for the copulatory organ, otherwise, female terminalia was usually relatively simple. This structure was reduced and formed a telescopic, eversible, and tubular ovipositor.

주사전자현미경을 이용한 무당벌레에 기생하는 무당벌레기생파리(Degeeria separata)의 외부형태적 특징

윤영남,김남성,김기덕,박수진,서미자,채순용 충남대학교 농업과학연구소 2000 농업과학연구 Vol.27 No.1

External morphological characteristics of male and female of Degeeria separata, which was endoparasited in adult of the Asian ladybird, were studied by using the scanning electron microscopy(SEM). The antenna of Degeeria separata was consisted of three segment and arista. Anstae were about 750㎛ length and 30㎛ in diameter. The surface of antennae was covered with many sensilla. They were long or short, slender or thick, and straight or curved. Each three morphological types of sensilla were observed on the second and third segment of antennae. The mouthpart was sponging type. It was consisted with clypeus, labellum, labium, labrum, and maxillary palp. The labellum is 400㎛ width and double folded vertically. The leg consist of tarsus, tarsus claw, pulvilli, and empodium.