배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

KRC 408, inhibitor of hepatic growth factor receptor (c-Met) suppress growth of gastric cancer through antiproliferation and antianigogenic antitumor activity

ZHENG Hong-Mei,HONG Sang-Won,CHOI Myung-Joo,KIM Da-Young,AHN Su-Mi,SHIN Moon-Geun,CHO Sung-Yun,KIM Hyoung-Rae,LEE Jong-Kook,LEE Don-Haeng,HONG Soon-Sun 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1

Improved Mutual MRAS Speed Identification Based on Back-EMF

Hong Zheng,Jiancheng Zhao,Liangzhong Liu 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.3

In the design of sensorless control system for induction motor, high-precision speed estimation is one of the most difficult problems. To solve this problem, the common method is model reference adaptive method (MRAS). MRAS requires accurate motor parameters to estimate rotor speed precisely. However, when motor is running, the variety of temperature and magnetic saturation will lead to the change of motor parameters such as stator resistance and rotor resistance, which will lower the accuracy of the speed estimation. To improve the accuracy and rapidity of speed estimation, this paper analyses the mutual MRAS speed identification based on rotor flux linkage, and proposes an improved mutual MRAS speed identification based on back-EMF. The improved method is verified by Simulink simulation and motor experimental platform based on DSP2812. The results of simulation and experiment indicate that the method proposed by this paper can significantly improve the accuracy of speed identification, and speed up the response of identification.

Porous silicon nanoparticles for cancer photothermotherapy

Hong, Chanseok,Lee, Jungkeun,Zheng, Hongmei,Hong, Soon-Sun,Lee, Chongmu Springer 2011 Nanoscale research letters Vol.6 No.1

<P>The <I>in vitro </I>cell tests and <I>in vivo </I>animal tests were performed to investigate the feasibility of the photothermal therapy based on porous silicon (PSi) in combination with near-infrared (NIR) laser. According to the Annexin V- fluorescein isothiocyanate Apoptosis assay test results, the untreated cells and the cells exposed to NIR laser without PSi treatment had a cell viability of 95.6 and 91.3%, respectively. Likewise, the cells treated with PSi but not with NIR irradiation also had a cell viability of 74.4%. Combination of these two techniques, however, showed a cell viability of 6.7%. Also, the cell deaths were mostly due to necrosis but partly due to late apoptosis. The <I>in vivo </I>animal test results showed that the Murine colon carcinoma (CT-26) tumors were completely resorbed without nearly giving damage to surrounding healthy tissue within 5 days of PSi and NIR laser treatment. Tumors have not recurred at all in the PSi/NIR treatment groups thereafter. Both the <I>in vitro </I>cell test and <I>in vivo </I>animal test results suggest that thermotherapy based on PSi in combination with NIR laser irradiation is an efficient technique to selectively destroy cancer cells without damaging the surrounding healthy cells.</P>

Inside Back Cover: Direct Patterning and Biofunctionalization of a Large-Area Pristine Graphene Sheet (Chem. Asian J. 3/2015)

Hong, Daewha,Bae, KiEun,Park, Duckshin,Kim, Houngkyung,Hong, Seok-Pyo,Kim, Mi-Hee,Lee, Bong Soo,Ko, Sangwon,Jeon, Seokwoo,Zheng, Xu,Yun, Wan Soo,Kim, Yang-Gyun,Choi, Insung S.,Lee, Jungkyu K. Wiley (John WileySons) 2015 Chemistry - An Asian Journal Vol.10 No.3

中国琼剧艺术发展的若干思考

Zheng Hong 동방문화대학원대학교 문화예술콘텐츠연구소 2020 문화와예술연구 Vol.16 No.-

경극(琼剧)은 기세차고 폭넓은 중국 전통문화의 배경하에 해 남에 전해진 중국 희곡의 여러 연극 종류가 충돌되면서 교류하 고 융합되고, 특히 월극(粤剧) 및 영남의 민속풍습과 융합된 후 지역의 민간소조(小调), 가무팔음(歌舞八音), 도운악곡(道坛乐 曲) 등 해남의 해남 문화요소를 흡수하였다. 이를 기반으로 하 여 경제 문화 융합의 배경하에 지역 방언을 위주로 하고 지속 적인 발전을 거쳐 최종적으로 우세 보완과 상부상조를 구현하 여 점차적으로 형성된 일종의 지역문화가 농후하고 지역적 특 색이 있는 해남도 본토 출신의 새로운 연극 종류이며, 중국 희 곡 백화원에 활짝 핀 하나의 기이한 꽃이다. 경극은 본토 대중들로부터 사랑을 받고 해남 지역에서 널리 유전되며 대중들이 즐겨 듣고 일상생활을 풍부하게 하며 영적 수요를 충족시키는 중요한 문화 형식이다. 오늘에까지 발전해 오면서 중국 해남도의 유일무이한 음악형식이 되었으며 해남음 악문화의 한 부호이다. 본 논문은 주로 경극의 무대공연형식 및 그 예술에 대해 자 세히 탐구하고자 한다. Qiongju is a wonderful type of Hainan local drama featured by local dialect and culture, blossoming in the area of Chinese opera under the broad background of Chinese traditional culture. After the collision, exchange and fusion of various kinds of Chinese operas introduced into Hainan, especially Cantonese opera and Lingnan folk customs, it has absorbed the local folk tunes, “Bayin”song and dance, Taoist music and other Hainan cultural elements. Qiong Opera is popular with local people and widely spread in Hainan area. It is an important cultural form for the public to enjoy and enrich their daily life and meet their spiritual needs. So far it has developed into a unique form of music in Hainan Island, China, and it is a symbol of Hainan music culture. This paper mainly discusses the stage performance form and its art in detail.

Two-step enhanced cancer immunotherapy with engineered <i>Salmonella typhimurium</i> secreting heterologous flagellin

Zheng, Jin Hai,Nguyen, Vu H.,Jiang, Sheng-Nan,Park, Seung-Hwan,Tan, Wenzhi,Hong, Seol Hee,Shin, Myung Geun,Chung, Ik-Joo,Hong, Yeongjin,Bom, Hee-Seung,Choy, Hyon E.,Lee, Shee Eun,Rhee, Joon Haeng,Min, American Association for the Advancement of Scienc 2017 Science Translational Medicine Vol.9 No.376

<P>We report a method of cancer immunotherapy using an attenuated Salmonella typhimurium strain engineered to secrete Vibrio vulnificus flagellin B (FlaB) in tumor tissues. Engineered FlaB-secreting bacteria effectively suppressed tumor growth and metastasis inmousemodels and prolonged survival. By using Toll-like receptor 5 (TLR5)-negative colon cancer cell lines, we provided evidence that the FlaB-mediated tumor suppression upon bacterial colonization is associated with TLR5-mediated host reactions in the tumor microenvironment. These therapeutic effects were completely abrogated in TLR4 and MyD88 knockout mice, and partly in TLR5 knockout mice, indicating that TLR4 signaling is a requisite for tumor suppression mediated by FlaB-secreting bacteria, whereas TLR5 signaling augmented tumor-suppressive host reactions. Tumor microenvironment colonization by engineered Salmonella appeared to induce the infiltration of abundant immune cells such as monocytes/macrophages and neutrophils via TLR4 signaling. Subsequent secretion of FlaB from colonizing Salmonella resulted in phenotypic and functional activation of intratumoral macrophages with M1 phenotypes and a reciprocal reduction in M2-like suppressive activities. Together, these findings provide evidence that nonvirulent tumor-targeting bacteria releasing multiple TLR ligands can be used as cancer immunotherapeutics.</P>

Anticancer effects of KI-10F: A novel compound affecting apoptosis, angiogenesis and cell growth in colon cancer

HONG, SANG-WON,JUNG, KYUNG HEE,CHOI, MYUNG-JOO,KIM, DA YOUNG,LEE, HEE-SEUNG,ZHENG, HONG-MEI,LI, GUANG YONG,EL-DEEB, IBRAHIM M.,PARK, BYUNG SUN,LEE, SO HA,HONG, SOON-SUN Spandidos Publications 2012 International journal of oncology Vol.41 No.5

<P>The anticancer effect of a new pyrazole derivative, KI-10F (2-(4-(2-(4-(dimethylamino) phenyl)pyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol?1-yl) acetonitrile)?3.5HCl) was evaluated in human colon cancer cells. KI-10F strongly suppressed the growth of human colon cancer cells and induced apoptosis by increasing the proportion of sub-G1 presenting apoptotic cells as well as causing cell cycle arrest at the G2/M phase. Apoptosis by KI-10F was confirmed by observation of an increase in the expression of cleaved caspase-3, caspase-8, caspase-9 and Bax, and the decrease of Bcl-2. Decreased expression of HIF-1α and VEGF, and the inhibition of HUVEC tube formation and migration showed that KI-10F effectively inhibited the angiogenesis process. Furthermore, in?vivo study in a mouse xenograft model showed that KI-10F produced a stronger antitumor activity than 5-FU, a conventional anticancer drug prescribed for the treatment of colon cancer. The effects of KI-10F on tumor proliferation (PCNA), angiogenesis (CD34) and apoptosis (cleaved caspase-3) were evaluated by immunohistochemistry using isolated tumor tissue samples. Taken together, our results demonstrated that KI-10F induces apoptosis and inhibits cell growth and angiogenesis both in?vitro and in?vivo. We suggest that KI-10F is an effective chemotherapeutic candidate for use against colon cancer.</P>

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

Effects of SRBSDV-infected rice plants on the fitness of vector and non-vector rice planthoppers

Hong-Xing Xu,Hong-Xing Xu,Xu-Song Zheng,Yajun Yang,Jue-Feng Zhang Zhang,Zhong-Xian Lu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Southern rice black-streaked dwarf virus (SRBSDV) is a new member of the genus Fijivirus and transmitted by whitebacked planthopper (WBPH), Sogatella furcifera (Horváth). The rice area affected by SRBSDV and the subsequent damages are increasing rapidly. In current study, we evaluated the effects of SRBSDV-infected plants on the ecological fitness and the tolerance to adverse environmental factors of vector WBPH and non-vector brown planthopper (BPH), Nilaparvata lugens (Stål). Our study revealed that the fitness of WBPH and BPH were both influenced by feeding on SRBSDV-infected rice. Fecundity ofWBPH feeding on the SRBSDV-infected rice plants were higher than those on healthy plants, nymphal duration of male WBPH prolonged markedly by 0.77 d (P b 0.05), while the weight of brachypterous female adults significantly decreased (P b 0.05), and the average survival time shortened both at 26 °C and 31 °C, respectively. Median lethal time of WBPH on SRBSDV-infected and healthy rice plants were 80 h and 112 h at 26 °C, 64 h and 88 h at 31 °C, respectively. As for non-vector BPH, nymph survival rate decreased significantly by 11.22% (P b 0.05) and the longevity of female adults also shortened (P b 0.05), while hatchability of BPH eggs increased (P b 0.05). Survival time of BPH feeding on healthy rice at 26 °Cwas significant longer than those feeding on healthy and infected rice at 31 °C. The above results imply that the vectorWBPH suffers much stronger effects than non-vector BPH fromfeeding on SRBSDVinfected rice plants.