Dextranase 함유 구강 세정액의 치태 제거 및 치은염 예방 효과에 관한 임상적 연구

송우성,손성희,정현주 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

A novel glucanhydrolase (DXAMase) from a mutant of Lipomyces starkeyi (KSM 22) has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and DXAMase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi DXAMase are desirable for its application as a dental plaque control agent. This study was performed to determine the adjunctive oral hygiene benefits and safety of dextranase (Lipomyces starkeyi KSM 22 DXAMase)-containing mouthwash when used alongside normal toothbrushing. This 6-month clinical trial was placebo-controlled double-blind design evaluating 1U/㎖ dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 3 and 6 months, subjects were scored for plaque accumulation(Turesky modification of Quigley-Hein's plaque index), gingivitis status (Lo¨e and Silness gingival index), and tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects were provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice daily after toothbrushing. All the groups showed significant increase in plaque accumulation since 1 month of experiment. During 6 month's period, the Dextranase mouthwash group showed the least increase in plaque accumulation, compared to the Chlorhexidine mouthwash and placebo groups. As for gingival inflammation, all the groups showed significant increase during 6 months of experiment. The Experimental group (Dextranase mouthwash) also showed the least increase in gingival index score, compared to the Positive control (Chlorhexidine mouthwash) as well as the Negative control (placebo) groups. Whereas the tooth stain was increased significantly in the Positive control group, compared to the baseline score and the Negative control group since 3 months of mouthrinsing. It was significantly increased after 6 months in the Experimental group, still less severe than the Positive control group. As for the oral side effect, the Experimental group showed less tongue accumulation, bad taste, compared to the Positive control group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase provided adjunctive benefits to toothbrushing, comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, with long-term use of the mouthwash. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.

치주질환 병인균간의 성장억제 활성도에 관한 연구

정해원,이상철,정종평,손성희 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.1

In a complex ecosystem such as periodontal pocket, bacterial antagonism may be a determinant in oral colonization and compositional changes of periodontopathic microflora. This study was performed to examine the growth inhibitory activities among the periodontopathic and indigenous bacteria. The growth inhibitory activity was examined as both producers and indicators by stab culture method. Inhibitory activities of cell sonicate and culture supernatant of S. mutans OMZ 65, which showed broad and strong inhibition against periodontopathic bacteria, was examined by the diffusion method. The results were as follows. 1. B. gingivalis, B. intermedius and A. actinomycetemcomitans strains showed weak inhibition against homologous and heterologous strains. Two among three strains of B. intermedius showed moderate inhibition against one strain of B. gingivalis and all of A. actinomycetemcomitans strains showed inhibitory activity to B. gingivalis. 2. The strains of S. mutans showed broad and strong inhibition against B. gingivalis, A. actinomycetemcomitans strains and moderate inhibition against B. intermedius, but S. sanguis strains showed weaker growth inhibitory spectra than those of S. mutans strains. However, the pattern of inhibitory activity showed, to a certain extent, species-specific. 3. S. mutans strains showed broader inhibitory spectrum against A. a. Korean strains than that of S. sanguis strains. But A. actinomycetemcomitans Korean strains has rarely showed growth inhibition against S. sanguis. 4. A. actinomycetemcomitans Korean strains showed weak inhibitory activity to B. gingivalis and no inhibitory activity to B. intermedius. 5. S. mutans showed strong inhibitory activity to homologous strains and typically species-specific than these of S. sanguis. Further investigation should be needed to clarify a relationship between the presence of plasmids and bacteriocin activity.

한국인 연령에 따른 Actinobacillus actinomycetemcomitans의 발현빈도 및 항체 역가에 관한 연구

정종평,김강주,이상철,손성희 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.2

In order to investigate on the frequency and antibody levels to Actinobacillus actinomycetemcomitans from Korean population, normal group were divided into 5 category by the related age : 3-6 years, 7-12 years, 13-20 years, 21-35 years and over 36 years, and also disease group were divided into the diagnostic condition : Localized juvenile periodontitis, post-localized juvenile periodontitis, rapidly progressive periodontitis, adult periodontitis. Each group was composed of 10 to 20 patients and plque sampling was also performed on the mesial pocket of mandibular lst molar with 3 paper points on anaerobic condition. Isolation and identification were done by using a selective medium(tryptic soy agar supplemented with 10% serum, 75ug of Bacitracin and 5ug of Vancomycin per ml) in 10% CO_2 incubator for 3 days with routine Gram staining, biochemical test and colony morphology. Serum antibody levels were also performed with isolated A. actinomycetemcomitans SNUDC 10-1 by using modified ELISA technique. The frequency of A. actinomycetemcomitans in normal healthy group was within 10% and no detection was revealed in 7 years group. Otherwise, the frequency of localized juvenile, rapidly progressive, adult perioduntitis and adult periodontitis associated with diabetes mellitus were 75%, 68%, 42%, and 25%, respectively. Serum IgG antibody levels to A.a SNUDC 10-1 in umbrical cord blood was similar to 3-6 years or 13-20 years group, and 3-6 months group were shown only trace amount of antibody levels. The antibody levels of 1 year and 7-12 years group were revealed 2/4-1/3 levels of 3-6 years group and this levels were gradually increased from 7-12 years group until over 36 years group. However, the antibody levels of localized juvenile, post-localized juvenile and rapidly progressive periodontitis were significantly higher than that of healthy group and no significant differences of the level were recognized between each disease group.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

HARMONIC HOMOMORPHISMS BETWEEN TWO LIE GROUPS

( Heui Sang Son ),( Hyun Woong Kim ),( Joon Sik Park ) 호남수학회 2016 호남수학학술지 Vol.38 No.1

In this paper, we get a complete condition for a group homomorphism of a compact Lie group with an arbitrarily given left invariant Riemannian metric into another Lie group with a left invariant metric to be a harmonic map, and then obtain a necessary and su±cient condition for a group homomorphism of (SU(2); g) with a left invariant metric g into the Heisenberg group (H; h0) to be a harmonic map.

Feasibility Evaluation of the Efficient Vermistabilization for Organic Waste Using Orp and Alkalinity as a Feed Condition

Heui Jeong Son,Sang An Ha,Nak Chang Sung,Hyeong Seok Kim,Yong Heui Lee 대한환경공학회 1998 Environmental Engineering Research Vol.4 No.4

HARMONIC HOMOMORPHISMS BETWEEN TWO LIE GROUPS

Son, Heui-Sang,Kim, Hyun Woong,Park, Joon-Sik The Honam Mathematical Society 2016 호남수학학술지 Vol.38 No.1

In this paper, we get a complete condition for a group homomorphism of a compact Lie group with an arbitrarily given left invariant Riemannian metric into another Lie group with a left invariant metric to be a harmonic map, and then obtain a necessary and sufficient condition for a group homomorphism of (SU(2), g) with a left invariant metric g into the Heisenberg group (H, $h_0$) to be a harmonic map.

A DECOMPOSITION OF THE CURVATURE TENSOR ON SU (3)/T (k, l) WITH A SU (3)-INVARIANT METRIC

Heui-Sang Son,Joon-Sik Park,Yong-Soo Pyo 충청수학회 2015 충청수학회지 Vol.28 No.2

In this paper, we decompose the curvature tensor (field) on the homogeneous Riemannian manifold SU (3)/T (k, l) with an arbitrarily given SU (3)-invariant Riemannian metric into three curvature-like tensor fields, and investigate geometric properties.

A DECOMPOSITION OF THE CURVATURE TENSOR ON SU(3)=T (k, l) WITH A SU(3)-INVARIANT METRIC

Son, Heui-Sang,Park, Joon-Sik,Pyo, Yong-Soo Chungcheong Mathematical Society 2015 충청수학회지 Vol.28 No.2

In this paper, we decompose the curvature tensor (field) on the homogeneous Riemannian manifold SU(3)=T (k, l) with an arbitrarily given SU(3)-invariant Riemannian metric into three curvature-like tensor fields, and investigate geometric properties.

Lithium 및 Carbamazepine이 Apomorphine으로 유도된 일측 흑질체 파괴 백서의 회전운동에 미치는 영향

손희섭,은홍배,유상은 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.2

This study was designed to evaluate the effects of haloperidol, lithium and carbamazepine on the apomorphine-induced turning behavior in the substantia nigra-lesioned rats with 6-hy-droxydopamine(6-OHDA). All rats were administered 8㎍ of 6-hydroxydopamine into the Rt. substantia nigra and were divided into seven groups. Each group was composed of six rats and was administered apom orphine, haloperidol(0.03㎎/㎏, 0.3㎎/㎏), lithium(2㎎/㎏, 20㎎/㎏) and carbamazepine(2㎎/㎏, 20㎎/㎏) respectively once a week for 7 weeks. The authors examined the effects of haloperidol, lithium and carbamazepine on the apomorphine-induced turning behavior. The results were as follows : 1) Haloperidol inhibited the turning behavior response induced by apomorphine significantly. 2) Lithium inhibited the turning behavior response induced by apomorphine significantly. 3) Carbamazepine did not influence the turning behavior response induced by apomorphine. From the above results, it is suggested that haloperidol and lithium inhibits the dopaminergic activity, but carbamazepine dose not influence on the dopaminergic activity.