Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

외식업체에서의 공급자-구매자 간 정보기술 활용에 관한 연구

강다원 제주관광대학 2004 論文集 Vol.10 No.-

본 연구적 목적은 정보기술의 발달로 인한 공급자-구매자 관계변화에 관한 연구 동향을 살펴보고, 국내 외식업계의 정보기술 활용에 대한 연구를 함으로써, 날로 발전하는 외식업체 경영정보 기술 도입을 위한 초석을 다지는데 있다. 외식업체에서의 공급자-구매자 관계는 적대적 관계에서 협력적 관계로, 시장거래적 관계에서 구매자 지배적관계, 더 나아가 전략적 파트너쉽으로 발전하고 있다. 한편 외식업체에서 현재 활용하고 있는 정보기술로는 POS System이 대표적이며, 구매 업무에 있어서는 인터넷 상용화 이후 큰 변화가 일고 있다. 전화나 팩스, 호텔자체 VAN을 이용한 구매업무에서 웹기반 구매-발주시스템이 점자 확대되고 있다. 2002년 말부터 대모 외식업체에서 웹기반 구매시스템이 도입되었으며, 최근 2004년에는 대규모 단체급식업체나 패밀리 레스토랑등 대형 외식업체들이 ERP(전자적 자원관리시스템 : Enterprise Resource Planning System)을 도입하고 있다. 앞으로의 후속연구는 본 연구를 바탕으로 ERP 시스템을 넘어, 외식업계에서의 공급사슬관리 (SCM : Supply Chain Management)에 관한 연구가 있어야 될 것이다.

사무직 근로자의 수면의 질 및 직무스트레스와 우울과의 관련성

강미나,강수영,권수정,김현주,배재원,이보연,이예진,임의롬,정다영,조한솔 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to understand the degree of depression, sleep quality, job stress and their association among office workers. Method: A self-administered questionnaire was distributed to 147 workers employed in 5 big enterprises, and 5 small and medium enterprises from October 21 to November 21, 2012. The questionnaires included socio-demographic and health-related characteristics, job-related characteristics, sleep quality, job stress (KOSS) and depression (CES-D). Result: Among all subjects, 23.1% was in the depression group (21 points and over in the CES-D score). Depressive level was positively correlated with job stress and quality of sleep. The adjusted odd ratio for the effects of sleep quality and job stress on depression significantly increased in the depression group compared to that of the normal group. Multiple regression analysis revealed that the following factors of influence had combined explanatory powers on depression: BMI, life satisfaction, quality of sleep, and job stress. Conclusion: The study revealed that complicated influences were exerted on the level of depression by variable factors, as well as socio-demographic characteristics, health-related characteristics, quality of sleep, and job stress. Specifically, the level of depression was influenced by the quality of sleep and job stress.

교내 실내 공기에서 부유 미생물의 분포

박창은,임미소,이다은,김설아,강재원 남서울대학교 2010 남서울대학교 논문집 Vol.16 No.2

The pathogenic and non-pathogenic microorganism have been isolated from the indoor airborne, the closest environment of human beings. Our purpose were to investigate the distribution of fungi and bacteria in indoor airborne sampled from 7 locations in Namseoul University for 2 months from September to October, 2010. The isolated bacteria were identified by Gram stain and biochemical test using VITEKTM system, also The fungus were identified by LPCB. The experiment on microorganism concentration of indoor air carried out and the average of total microorganism was measured. When airborne bacteria were isolated and identified. The isolation rates of Gram positive cocci, Gram positive bacilli, Gram negative bacilli, and Gram negative cocci were 38.3%, 22.4%, 2.7%, 0%, respectively. In Gram positive cocci, the most strains were identified as Micrococcus spp. In Gram positive bacilli, the most strains were identified as Bacillus spp. The frequently isolated fungi were Aspergillus sp, Penicillium sp, Alternaria sp, Rhizopus sp, Mucor sp. respectively. Further systematic studies are necessary with an emphasis on species identification. Key Words : Microorganism, Fungus, Indoor Airborne, Micrococcus, Aspergillus

Upregulation of Glutathion S-Transferase mu 1 in Bovine Cystic Follicles

Kang, Da-Won,Kim, Chang-Woon,Han, Jae-Hee 韓國受精卵移植學會 2010 한국동물생명공학회지 Vol.25 No.4

Follicular cystic follicles (FCFs) show delayed regression with persistent follicle growth. However, the mechanism by which follicles are persistently grown remains unclear. Glutathione S-transferases (GSTs) are drug-metabolizing and detoxification enzymes that are involved in the intracellular transport and metabolism of steroid hormones. In this study, a proteomic analysis was performed to identify whether GST expression is changed in bovine FCFs and to predict the interactions between GST and other proteins. Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and 25 mm). In bovine follicles, GST mu 1 (GSTM1) was detected as a differentially expressed protein (DEP) and significantly up-regulated in FCFs compared to normal follicles (p<0.05). Consistent with the proteomic results, semi-quantitative PCR data and western blot analysis revealed an up-regulation of GSTM1 in FCFs. Expression levels of aromatase and dehydrogenase proteins were changed in FCFs. These results show that the up-regulation of GSTM1 that is observed in bovine FCFs is likely to be responsible for the persistent follicle growth in FCFs as the activity of aromatase and the dehydrogenases.

Effect of Soil Amendments on Arsenic Reduction of Brown Rice in Paddy Fields

Kang, Dae-Won,Kim, Da-Young,Yoo, Ji-Hyock,Park, Sang-Won,Oh, Kyeong-Seok,Kwon, Oh-Kyung,Baek, Seung-Hwa,Kim, Won-Il 한국토양비료학회 2018 한국토양비료학회지 Vol.51 No.2

There is an increasing concern over arsenic (As) contamination in rice since Codex Committee on Contaminants in Food (CCCF) discuss on maximum levels for As in rice in 2010. This study was conducted to reduce As concentration in rice by soil amendment treatments in paddy field soils contaminated by As. The selected four amendments were poultry manure, agri-lime, steel slag, and gypsum with the addition of 3% or 5% (w/w) on a dry basis. The As reduction effect could not be verified, as a result of the pot test by adding poultry manure to the paddy soil around the mine located in Yesan. Among the agri-lime treated rice cultivated pots, the As concentration increased up to 32.1%. On the other hand, the content of As in the sample pots treated with steel slag and gypsum decreased by 65.4% and 63.4%, respectively. On the basis of the results of these pot experiments, the field test was carried out in the As polluted rice field around the mine located in Yesan, and when the four amendments were treated, the As content in the brown rice reduced in all the amendment treatments compared with the control plot. The As reduction in brown rice of the amendment was confirmed to be higher efficiency by the order of gypsum > steel slag > poultry manure > agri-lime. As a result of pot experiments using paddy soil around the mine located in Seosan, As stabilization efficiency in rice and As reduction effect could not be determined by comparison to the control. From the rice cultivated from agri-lime treated pot, As concentration increased by 15.8% in rice. On the other hand, the As content of the pots treated with steel slag and gypsum decreased by 39.1% and 60.2%, respectively. In conclusion, distinguished As reducing effectiveness could be expected by soil amendment treatments for rice cultivation.

Acetylcholine-Induced Calcium Current and Oscillation in Mouse Eggs

Da-Won Kang,Jae-Hee Han,Seong-Geun Hong 한국동물번식학회 2002 Reproductive & Developmental Biology(Supplement) Vol.26 No.1s

Adverse Effects on Crops and Soils Following an Accidental Release of Hydrogen Fluoride and Hydrofluoric Acid

Kang, Dae-Won,Kim, Hyuck-Soo,Kunhikrishnan, Anitha,Kim, Da-In,Lee, Seul,Park, Sang-Won,Yoo, Ji-Hyock,Kim, Won-Il 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

A number of accidents relating to highly toxic hydrogen fluoride (HF) or hydrofluoric acid (HA) release have occurred over fast few decades in Korea. Thus, this study was conducted to investigate the fluoride (F) concentrations in paddy soil and brown rice from 2 different areas where the soils were exposed to HF and HA. In the first case, the HF leakage accident that occurred in 2012 affected the surrounding soils and crops and consequently, crops (rice) affected by HF were unavailable for forage even though F did not accumulate in the soil. For example, at the time of accident, F concentrations in brown rice samples were $33.0-1,395mg\;kg^{-1}$, while F concentrations in soil samples were $155-295mg\;kg^{-1}$ which were less than the Korean standard guideline values of $400mg\;kg^{-1}$. However, after a year, F concentrations in brown rice were observed below the detection limit ($1mg\;kg^{-1}$), although F concentrations in soils were similar with those in 2012. Also, large amounts of wastewater discharges containing HA occurred in 2013 and some agricultural soils exceeded the Korean standard guideline values for F ($400mg\;kg^{-1}$), but soil-plant F transfer was not observed. In conclusion, it was observed that soil to plant transfer of F is unlikely although HF and HA as gas or liquid form can cause direct damage to plants.

Identification of Differentially Expressed Genes between Neonatal and Peripubertal Rat Thymi Using $GeneFishing^{TM}$ Polymerase Chain Reaction

Kang, Da-Won,Kim, Gyu-Tae,Han, Jae-Hee The Korean Society of Animal Reproduction 2007 Reproductive & developmental biology Vol.31 No.1

Aging causes thymus involution, and genes in thymus play an important role in the development of the immune system. In this study, we compared genes expressed in thymus of neonatal and peripubertal rats using annealing control primers (ACPs)-based GeneFishing polymerase chain reaction (PCR) and semiquantitative reverse transcription (RT)-PCR. We identified 10 differentially expressed genes (DEGs) with 20 ACPs. Of 10 DEGs, bystin-like, collagen type V alpha 1 (COL5A1), and T-cell receptor beta-chain segment 2 (TCRB2) that are related to immune-function were detected in rat thymus. Bystin-like and TCRB2 were up-regulated, while COL5A1 was down-regulated in peripubertal thymus. Semiquantitative RT-PCR confirmed postnatal changes in expression of bystin-like, COL5A1, and TCRB2. These results suggest that bystin-like, COL5A1, and TCRB2 could regulate immune function controlled in thymus as age increases.

Ca and concentrations change during early embryonic development in mouse

Kang Da-Won,Hur Chang-Gi,Choi Chang-Rok,Park Jae-Yong,Hong Seong-Geun,Han Jae-Hee 한국발생생물학회 2005 한국발생생물학회 학술발표대회 Vol.2005 No.1