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사료내 단백원의 차이가 참돔(Pagrus major)과 교잡어 (P. major ♀×Acanthopagrus schlegeli ♂)의 성장과 에너지수지에 미치는 영향
김양수,Biswajit Kumar Biswas,Amal Biswas,지승철,정관식,Osamu Murata,Yoshifumi Sawada,Kenji Takii 전남대학교 수산과학연구소 2013 수산과학연구소논문집 Vol.21 No.-
This study investigated the growth and energy partition of juvenile red sea bream Pagrus major (159.3±9.5 g; RSB) and hybrid sea bream P. major (♀)×Acanthopagrus schlegeli (♂) (144.8± 10.0 g; F1) on satiation feeding of fish meal (FM) and soybean meal (SM) based isocaloric diets (21.1 kJ/g diet) at 21.6℃ water temperature for 4 weeks. Two sea bream species offered no significant influence on final mean body weight, condition factor, viscera somatic index and apparent digestibility coefficient in both dietary groups, and both fish species were increased the relative stomach and intestine weight in SM than that in FM. RSB fed FM diet showed larger growth rate, feed efficiency, protein efficiency ratio, and apparent nutrients and energy retention efficiency than that fed SM diet, but this was inverse in F1. Whereas, Energy partitions of RSB and F1 in FM and SM dietary groups observed a similar tendency on fecal, digestible, urinary, metabolizable and standard metabolizable energy. However, retained energy of RSB in fed FM diet higher than that in SM, was inverse in F1. Moreover, heat increment plus voluntary activity energy consumption of F1 fed SM diet compare with fed FM diet lower than that of RSB. These results suggest that RSB and F1 has similar the energy consumption metabolism for digestive absorption in both diets, however, the energy accumulation pattern from absorbed nutrients appear to differ on SM fed.
Sarkar Kishore Kumar,Rahman Md. Muzibar,Shahriar Abdullah Al Ehsan,Mitra Trina,Golder Mimi,Zilani Md. Nazmul Hasan,Biswas Biswajit 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.3
Mimusops elengi Linn. is traditionally used as antianxiety, cytotoxic, antimicrobial, antioxidants etc. Also, conventionally, Alstonia scholaris Linn. is used in treating cancer, psychosis, nociception etc. The present study was designed to appraise neuropharmacological and cytotoxic potential of M. elengi and A. scholaris leaves. Hole cross and hole board tests were conducted for assessing sedative effect, thiopental sodium induced sleeping time test for hypnotic property, elevated plus maze (EPM) test for anxiolytic potential, tail suspension test for antidepressant effect and finally brine shrimp lethality bioassay for evaluating cytotoxic potential of both extracts. In both hole cress and hole board tests, M. elengi leaves (MEL) displayed greater locomotion reduction compared to A. scholaris leaves (ASL). Besides, in thiopental sodium induced sleeping time and EPM test, both extracts confirmed significant (p < 0.05) hypnotic and anxiolytic potential where MEL showed better effectiveness than ASL. Moreover, an increase in the duration of immobility after oral administration of extracts in tail suspension test depicts no antidepressant effect rather a depressing action. Finally, both extracts exhibited potent cytotoxic property in brine shrimp lethality bioassay where the LC50 values for ASL and MEL were 61.85 and 67.2 µg/ml, respectively. So, ASL and MEL extracts revealed significant neuropharmacological impact as well as impressive cytotoxic potential that justify the traditional uses of both plants.
Mimi Golder,Samir Kumar Sadhu,Biswajit Biswas,Tannami Islam 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3
Ethanolic extracts of leaves and hypocotyls of a mangrove plant, Bruguiera gymnorrhiza (Family Rhizophoraceae), were investigated to identify the presence of different phytochemicals and to assess the antihyperglycemic, antidiarrheal, analgesic and antioxidative activity in the current study. Oral glucose tolerance test (OGTT) was performed to investigate the in vivo antihyperglycemic activity and leaves extract (LE) was found to possess significant antihyperglycemic property compared to hypocotyl extract (HE). Therefore, the leaves extract was considered for α-Glucosidase enzyme inhibitory activity test and substantial α-glucosidase enzyme inhibitory property was observed with an IC50 (concentration required to inhibit 50% enzyme) value of 2.670. Antioxidative activity of plant extracts was tested in vitro by evaluating their ability to scavenge DPPH, hydrogen peroxide and hydroxyl radical as well as superoxide anions. In all cases, LE showed greater activity than HE. In addition, LE demonstrated higher amount of total phenolics, flavonoids and tannins indicating higher antioxidant effect compared to HE. For the evaluation of antidiarrheal effect in castor oil induced diarrheal mice, both extracts showed significant inhibition of defecation at doses 250 and 500 mg/kg body weight. Finally, for acetic acid-induced writhing method to evaluate the analgesic activity, significant percentage inhibition of writhing (P < 0.05 vs. control) was observed for both extracts at lower and higher doses. Altogether, these studies justified the plant’s usage in folklore medication.
Kim, Yang-Su,Ji, Seung-Cheol,Biswas, Amal,Biswas, Biswajit Kumar,Yong, Annita Seok Kian,Takaoka, Osamu,Jeong, Gwan-Sik,Murata, Osamu,Takii, Kenji The Korean Society of Fisheries and Aquatic Scienc 2014 Fisheries and Aquatic Sciences Vol.17 No.1
To determine a suitable dietary protein/lipid (CP/CL) ratio in the early juvenile stages of hybrid porgy ($F_1$), female red sea bream (RSB) ${\times}$ male black sea bream, five diets with various CP/CL ratios-60/7, 55/12, 51/17, 46/23, and 41/28-were prepared and provided to juveniles in triplicate. At the smaller juvenile stage, $F_1$, weighing 0.32 g, a significantly higher specific growth rate (SGR) and feed efficiency (FE) were seen with 60/7 and 55/12 diets. However, in RSB weighing 0.26 g, SGR and FE were higher with the 60/7 diet than the other diets at $21^{\circ}C$. At the larger juvenile stage, $F_1$, weighing 3.7 g, there was no significant difference in SGR or FE among the diets, but RSB weighing 4.0 g fed 60/7, 55/12, and 51/17 diets had higher SGR and FE than 46/23 and 41/28 diets at $24^{\circ}C$. Moreover, survival and apparent nutrient retention of $F_1$ at both stages were significantly higher than those in RSB. These results indicate that both $F_1$ and RSB weighing ca. 0.3 g require a higher dietary CP/CL than those weighing ca. 4 g. Additionally, $F_1$ in both trials showed the suitability of a lower dietary CP/CL than RSB, indicating that mass production of $F_1$ juveniles will be more economical than RSB.
( Yang Su Kim ),( Seung Cheol Ji ),( Amal Biswas ),( Biswajit Kumar Biswas ),( Annita Seok Kian Yong ),( Osamu Takaoka ),( Gwan Sik Jeong ),( Osamu Murata ),( Kenji Takii ) 한국수산과학회(구 한국수산학회) 2014 Fisheries and Aquatic Sciences Vol.17 No.1
To determine a suitable dietary protein/lipid (CP/CL) ratio in the early juvenile stages of hybrid porgy (F1), female red sea bream (RSB) × male black sea bream, five diets with various CP/CL ratios「60/7, 55/12, 51/17, 46/23, and 41/28」were prepared and provided to juveniles in triplicate. At the smaller juvenile stage, F1, weighing 0.32 g, a significantly higher specific growth rate (SGR) and feed efficiency (FE) were seen with 60/7 and 55/12 diets. However, in RSB weighing 0.26 g, SGR and FE were higher with the 60/7 diet than the other diets at 21°C. At the larger juvenile stage, F1, weighing 3.7 g, there was no significant difference in SGR or FE among the diets, but RSB weighing 4.0 g fed 60/7, 55/12, and 51/17 diets had higher SGR and FE than 46/23 and 41/28 diets at 24°C. Moreover, survival and apparent nutrient retention of F1 at both stages were significantly higher than those in RSB. These results indicate that both F1 and RSB weighing ca. 0.3 g require a higher dietary CP/CL than those weighing ca. 4 g. Additionally, F1 in both trials showed the suitability of a lower dietary CP/CL than RSB, indicating that mass production of F1 juveniles will be more economical than RSB.
교잡어, 참돔 (Pagrus major, ♀) × 감성돔 (Acanthopagrus schregeli, ♂)의 성장 및 수온변화와 저염분 환경에서의 내성
김양수 ( Yang Su Kim ),지승철 ( Seung Cheol Ji ),( Biswajit Kumar Biswas ),( Amal Biswas ),정관식 ( Gwan Sik Jeong ),( Osamu Murata ),( Ken Ji Takii ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.3
The growth and tolerance for water temperature and salinity were compared among red sea bream Pagrus major (RSB) black sea bream Acanthopagrus schregeli (BSB) and their hybrid (F1), female RSB × male BSB. The growth of the F1 fish did not differ until 27 days after hatching (dAH), after which the most rapid growth was observed until approximately 300 dAH, followed by RSB and BSB. However, the RSB had out grown the F1 fish by approximately 303 dAH. By 480 dAH, the RSB were largest, followed by the F1 and BSB groups. The tolerances for high and low water temperature were significantly different for each species and growth stage. The largest tolerance spectrum was observed in the BSB group, and the tolerance spectrums gradually decreased with increasing final body weight. During the salinity tolerance trials, all of the species started to die following transfer into freshwater (0 psu). BSB showed the highest survival rate when kept in fresh water for an average of 29.9 hours, while the F1 fish were more tolerant than the RSB fish. The fish were increasingly tolerant to fresh water and changes in water temperature as they grew, while the size of the temperature spectrum remained unchanged but shifted to a lower temperature range with growth. Our results demonstrate that F1 performed well in terms of growth compared to parental fish, with higher temperature and salinity tolerances than RSB, and is thus suggested to be a suitable aquaculture species for Korea and northeast China.
Md. Naharul Islam,Hiron Saraj Devnath,Maisha Maliha Medha,Ritu Porna Biswas,Nripendra Nath Biswas,Biswajit Biswas,Samir Kumar Sadhu 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.3
Hill tract plant Tetrastigma bracteolatum (Wall.) (Family-Vitaceae) is traditionally used for headache, indigestion and stomach disorder by the tribal people in Bangladesh. In this study, the ethanolic leaves extract of TB was investigated for screening its phytochemical constituents and assessing the antioxidant, analgesic, antidiarrheal and antihyperglycemic potential. In DPPH free radical scavenging assay, the extract showed good radical scavenging potential with SC50 (concentration for 50% scavenging) value of 56 µg/ml. Analgesic activity test was performed by acetic acid-induced writhing method, where the extract inhibited writhing by 28.8% and 51.5% at the doses of 250 and 500 mg/kg bw, respectively. The extract inhibited defecation by 36.2% and 60.3% at the doses of 250 and 500 mg/kg bw in castor oil-induced diarrheal mice. In the evaluation of antihyperglycemic activity by oral glucose tolerance test (OGTT), the extract reduced blood glucose levels in a dose-dependent manner after 90 min, compared to the control group. The extract was also assessed for its in vitro α-glucosidase inhibition capacity but no demonstrable efect was observed. Fourteen chemical compounds were identifed through GC–MS analysis of the extract with the highest amount of 1,2,3,4,4a,5,8,9,12,12a-decahydro-1,4-methanobenzocyclodecene (5.46%). Few compounds displayed better binding afnity with the active site of NADPH oxidase, nitric oxide synthase, M3 muscarinic acetylcholine receptor and sulfonylurea receptor than standard drugs in molecular docking studies. Therefore, the extract of TB may be used as a potential source of drug leads for various ailments.