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Metabolomic profiling analysis for biomarker discovery in human disease
Geum-Sook Hwang(황금숙) 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Metabolomics is the technology of studying, profiling, and fingerprinting metabolites in various physiologic states. This approach has recently demonstrated enormous potentials in many fields such as genotype discrimination, toxicological mechanism, disease processes, and drug discovery. Metabonomic approaches have been used to generate comprehensive biochemical profiles of low molecular weight endogenous metabolites for biofluides and tissue. This metabolic profile is perturbed in a characteristic fashion in disease, toxic process and drug efficacy, and this shift in position can be readily visualized and modeled using chemometric techniques. The metabolic profile of biofluids shows changes of their composition in response to toxic or disease-induced stress due to the system"s attempt to maintain homeostasis. These methods constitute a metabolomic approach to study the quantitative metabolic consequences of patho-physiological insult. Understanding the biochemical reason for such a shift in metabolic space leads to the identification of biomarkers of disease or drug efficacy. ¹H NMR and Mass spectrometry (MS) were used to generate a molecular fingerprint of biofluid or tissue samples, and then pattern recognition technique was applied to identity molecular signatures associated with the specific diseases or drug efficiency. Several metabolites that differentiate disease samples from the control were thoroughly characterized and the metabolic changes in human and animal model were investigated using ¹HNMR and MS. Spectral data were applied to targeted profiling and spectral binning method, and then multivariate statistical data analysis (MVDA) was used to examine in detail the modulation of small molecule candidate biomarkers. The metabolic profiling produces robust models, generates accurate metabolite concentration data, and provides data that can be used to help understand metabolic differences between healthy and disease or drug treated models. Such metabolic signatures could provide diagnostic markers for a disease state or biomarkers for drug response phenotypes, and mechanistic information on cellular perturbations and pathways.
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한의학의 과학적 연구를 위한 대사체학(Metabolomics)에 대한 고찰
정지연 ( Jee Youn Jung ),황금숙 ( Geum Sook Hwang ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.4
Objective: This study is achieved to know the way that we can use metabolomics for the scientific research of Korean medicine. Results: We elucidate that metabolomics can be contributed to four ways for objectification of Korean medicine 1. Diagnosis of BeunJungLonChi(辯證論治). 2. Application toward Personalized medicine. 3. Quantification of Herbal medicine. 4. Evaluation of efficacy/safety for Korean medicine formulation Conclusion: Metabolomics opens up a new opportunity to reinvestigate Korean medicine from holistic perspective. Furthermore, Metabolomics is expected to provide effective method in research of acupuncture and meridian.
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성별 및 채뇨 시각별 <SUP>1</SUP>H NMR 기반 뇨 대사체 프로파일링 연구
정진영(Jin-Young Jeong),황금숙(Geum-Sook Hwang),박종철(Jong-Chul Park),김동현(Dong-Hyun Kim),하미나(Mina Ha) 환경독성보건학회 2010 환경독성보건학회지 Vol.25 No.4
Objectives : This study was undertaken to examine the metabolomic changes due to gender and diurnal variation at sampling time and to identify an appropriate time point for urine sampling in epidemiologic studies using metabolomic profiles. Methods : Urine samples were collected twice a day (morning and afternoon) from 20 healthy Korean adults after fasting for 8 hours. The metabolomic assay was investigated using H NMR spectroscopy coupled with the principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The metabolites responsible for differentiation between groups were identified through the loading plot of PLS-DA and quantified using Chenomx NMR Suite with a 600 ㎒ library. Results : Metabolites responsible for differentiation in gender and sampling time were creatinine, trimethyl anine oxide (TMAO), hippurate, mannitol, citrate and acetoacetate. Dimethylamine showed difference only as a factor of diurnal time. The level of creatinine was higher in men compared to women, and the levels of citrate, TMAO, hippurate, mannitol, and acetoacetate were higher in women compared to men. The levels of creatinine, TMAO, hippurate, dimethylamine and mannitol were higher in the morning rather than the afternoon while those of citrate and acetoacetate were higher in the afternoon rather than the morning. Conclusions : Since urinary metabolomic profiles varied by gender and diurnal cycle, urine sampling should be performed at the same time point for all participants in epidemiologic studies using metabolomic profiles.
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대사체학의 연구 동향, 응용 및 국내 연구 활성화 방안
김소현,양승옥,김경헌,김영석,유광현,윤영란,이동호,이충환,황금숙,정면우,최기환,최형균,Kim, So-Hyun,Yang, Seung-Ok,Kim, Kyoung-Heon,Kim, Young-Suk,Liu, Kwang-Hyeon,Yoon, Young-Ran,Lee, Dong-Ho,Lee, Choong-Hwan,Hwang, Geum-Sook,Chung, Myeon 한국생물공학회 2009 KSBB Journal Vol.24 No.2
대사체학은 동 식물, 미생물뿐만 아니라 식품, 농업, 의약품에 이르기까지 다양한 분야에서 적용될 수 있으며, 최근 미래를 선도할 학문으로서 주목 받고 있는 분야이다. 하지만 우리나라의 대사체학 연구는 아직 기초적 단계이며, 대사체학에 대한 인식도 부족한 상황이다. 따라서 본 논문에서는 대사체 연구 방법에 대해서 간단히 소개하였고, 국내 외 대사체학 연구현황, 대사체 연구의 필요성과 활용방안, 대사체 연구 수행 활성화를 위한 전략들을 소개하였다. 대사체학은 활용 범위가 매우 넓은 것이 특징인데, 예를 들어 functional genomics, 생물의 계통 분류, 생물의 대사경로 규명, 생물을 이용한 유용물질 생산, 신약 및 신소재 개발, biomarker의 개발, 식품 및 천연물 제제의 품질관리, 그리고 환경 및 독성 모니터링 등에 활용될 수 있다. 그러나 국내 대사체학 연구는 초기단계에 머물러 있는 실정이므로 국내 대사체 연구의 발전을 위해서는 연구 주체간의 협력, 해외 선진 기술 습득, 연구 개발 투자, 대사체 분석 전문가 육성, metabolome database 구축 등이 필요하다. 대사체학 연구에 대한 이러한 지원이 이뤄진다면, 대사체학 분야에 있어서 국내수준과 세계수준의 격차는 줄어들 것이다. 또한 결과적으로 대사체학 연구의 발전은 한국 생명공학 분야 (BT)의 발전에도 크게 이바지할 것으로 사료된다. As one of the new areas of 'omics' technology, there is increasing interest in metabolomics, which involves the analysis of low-molecular-weight compounds in cells, tissues, and biofluids, and considers interactions within various organisms and reactions of external chemicals with those organisms. However, metabolomics research is still at a fundamental stage in Korea. Therefore, the purpose of this study was to establish a strategic long-term plan to revitalize the national metabolomics approach and obtain the elementary data necessary to determine a policy for effectively supporting metabolomics research. These investigations clarified the state of metabolomics study both in Korea and internationally, from which we attempted to find the potentiality and fields where a metabolomics approach would be applicable, such as in medical science. We also discuss strategies for developing metabolomics research. This study revealed that promoting metabolomics in Korea requires cooperation with metabolomics researchers, acquisition of advanced technology, capital investment in metabolomics approach, establishment of metabolome database, and education of metabolome analysis experts. This would reduce the gap between the national and international levels of metabolomics research, with the resulting developments in metabolomics having the potential to greatly contribute to promoting biotechnology in Korea.
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