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청피(靑皮)가 뇌혈류와 복강내 Macrophage에 미치는 영향
김남순 ( Nam Soon Kim ),황경택 ( Kyung Taek Whang ),황일택 ( Il Taek Whang ),강덕수 ( Duk Soo Kang ),강학천 ( Hak Chun Kang ),한종현 ( Jong Hyun Han ) 대한경락경혈학회 2001 Korean Journal of Acupuncture Vol.18 No.2
Citri Reticulatae Viride Pericarpium is being used to regulate the flow of gi. However, the mechanism of its pharmacological actions is not well understood. The purpose of this research was to investigate effects of Citri Reticulatae Viride Pericarpium on peritoneal macrophages in mice and blood pressure, regional cerebral blood flow in rats. Nitric oxide production was tested using a Griess reagents, and a phagocytic activity of mouse peritoneal macrophage was tested using a luminometer. Blood pressure and regional cerebral blood flow was tested using a phygiograph, MacLab and Macintosh computer with data acquisition system. 1. Blood pressure was increased by Citri Reticulatae Viride Pericarpium in a dose-dependent manner. 2. rCBF was increased by Citri Reticulatae Viride Pericarpium in a dose-dependent manner. 3. Pretreatment with propranolol significantly inhibited Citri Reticulatae Viride Pericarpium induced increase in rCBF. 4. Citri Reticulatae Viride Pericarpium inhibited nitric oxide production from peritoneal macrophages in mice. 5. Citri Reticulatae Viride Pericarpium accelerated phagocytic activity of peritoneal macrophage in mice These results suggest that Citri Reticulatae Viride Pericarpium causes a diverse response of blood pressure, regional cerebral blood flow(rCBF), nitric oxide production and phagocytic activity. The increase in rCBF is also mediated by adrenergic βrecepter.
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황경택,경은호,나한일,소응향,최병화,한종현 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1
The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) in rats, following the intravenous injection of Catalpa Ovata Water Extract (COWE). The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure tranducer(Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as following : COWE increased the changes of rCBF and BP significantly. After pretreatment with propranolol and indomethacin, the changes of rCBF were inhibited significantly, but not by L-NNA and methylene blue. Also, after pretreatment with propranolol, the changes of BP were inhibited significantly, but not by L-NNA, methylene blue and indomethacin. According to above result, it is considered that Catalpa Ovata increased the rCBF and BP, related to adrenergic β-receptor.
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