Biorenovation 생물전환 기법을 이용한 비트 추출물의 LPS로 유도된 RAW 264.7 세포에 대한 항염증 효과

홍혜현(Hyehyun Hong),박태진(Taejin Park),강민성(Min-Sung Kang),김승영(Seung-Young Kim) 한국생물공학회 2021 KSBB Journal Vol.36 No.2

Biorenovation is a method of modifying the structure of a wide range of substrates such as chemical compounds and plant extracts by microbial enzymes, which can reduce cytotoxicity and improve biological activity. In this study, biorenovation was applied to Beta vulgaris (BV) to produce biorenovation product (BVB), and anti-inflammatory activity was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. As a result, BVB inhibited nitric oxide (NO) production at a non-toxic concentration in a concentration-dependent manner, and inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and pro-inflammatory cytokine interleukin-6 (IL-6), prostaglandin E₂ (PGE₂), interleukin-1β (IL-1β). Our study shows that BVB exhibits anti-inflammatory activity by inhibiting the production of various inflammatory mediators. Moreover, it suggests that biorenovation has potential usefulness in the development of anti-inflammatory drugs.

LPS로 유도된 R AW 264. 7 대식세포에서 Naringenin-7-O-phosphate의 항염증 활성

홍혜현 ( Hyehyun Hong ),박태진 ( Tae-jin Park ),최병민 ( Byeong Min Choi ),이유정 ( Yu-jung Yi ),김승영 ( Seung-young Kim ) 한국응용생명화학회 2023 Journal of Applied Biological Chemistry (J. Appl. Vol.66 No.-

플라보노이드는 광범위한 생물학적 활성을 가진 중요한 식물 2차 대사 산물로, 본 연구에서 사용된 naringenin (NN)은 자몽에 가장 풍부한 플라바논의 하나로 간 보호, 항지질 과산화 및 항암 활성에 관한 연구가 보고되었다. 우리는 이전 연구에서 biorenovation 기법을 NN에 적용하여 naringenin-7-O-Glucoside (prunin), naringenin-7-O-phosphate (N7P), 6''-O-Succinyl prunin과 같은 3가지 유도체를 합성하였으며 그 중 생물학적 및 물리화학적 특성이 보고되지 않은 N7P가 NN보다 45배 증가한 수용해도를 나타냄을 확인하였다. 따라서 본 연구에서는 N7P의 추가적인 생리활성 조사하고자 RAW 264.7 세포에서 항염증 활성을 평가하였으며 N7P는 세포 독성이 나타나지 않는 농도에서 유효한 inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) 억제 활성을 보였으며 이들의 발현 경로를 유의하게 억제함으로써 nitric oxide (NO) 및 prostaglandin E₂ (PGE₂)를 효과적으로 억제하였다. 뿐만 아니라 pro-inflammatory cytokines 인 tumor necrosis factor-α (TNF-α)와 interleukin-6 (IL-6)의 발현 또한 유의하게 억제하는 것으로 확인되었다. 이러한 결과를 근거로 N7P가 다양한 염증 인자를 표적으로 하는 항염증 소재로 적용될 수 있음을 제안한다. The most abundant flavanone of grapefruits, naringenin (NN), is well known for its hepatoprotective, anti-lipid peroxidation and anti-carcinogenic effects. We generated three derivatives from NN using this technique in previous studies. Among them, it was confirmed that naringenin-7-O-phosphate (N7P), whose biological and physicochemical properties were not reported, showed a water solubility 45 times higher than that of NN. Therefore, in this study, the anti-inflammatory activity was evaluated in RAW 264.7 cells to investigate the potential physiological activity of N7P. As a result, N7P showed nitric oxide (NO) inhibitory activity at concentrations that did not show toxicity. In addition, prostaglandin E₂ (PGE₂) showed significant inhibitory activity from the lowest concentration of 12.5 μM and showed increased inhibitory activity compared to NN. In addition, as a result of western blot, N7P showed increased cyclooxygenase-2 (COX-2) inhibitory activity than NN, and effectively inhibited NO and PGE₂ by significantly inhibiting their expression pathways. N7P also inhibited inflammatory cytokines, including tumor necrosis factor-α, interleukin-6. Based on these results, we propose that N7P can be used as a potent anti-inflammatory agent.

TV 의학 보도의 예시화 효과 분석

홍혜현(Hyehyun Hong) 한국언론학회 2015 한국언론학보 Vol.59 No.1

자생 Tuber himalayense 자실체 추출물의 항염증 활성

김민경,홍혜현,김정환,김승영,김창무,Kim, Minkyeong,Hong, Hyehyun,Kim, Jung-Hwan,Kim, Seung-Young,Kim, Changmu 한국버섯학회 2021 한국버섯학회지 Vol.19 No.3

본 연구는 국내 참나무 생육지에서 채집된 송로버섯의 일종인 T. himalayense 자실체 추출물(TH)의 항염증 활성을 확인하였다. LPS로 유도된 RAW 264.7 대식세포를 대상으로 한 염증 억제 실험에서 TH는 100 ㎍/ml 이하의 농도에서 세포독성을 보이지 않았으며 LPS에 의해 증가된 NO와 PGE₂의 생성을 농도 의존적으로 억제하였다. Western blot 분석 결과로 볼 때, TH의 항염증 활성은 iNOS와 COX-2 유전자의 발현억제에 의해 NO와 PGE₂의 생성이 감소된 것으로부터 유발된 것임을 입증한다. 또한, TH가 대식세포에 의해 생산 및 분비되는 cytokine IL-1β와 IL-6의 생성을 효과적으로 저해함을 확인하였다. T. himalayense는 다양한 염증 매개체들의 과발현을 효과적으로 억제하며, 이를 표적으로 하는 항염증 관련 식품 및 의약품에 다양하게 활용 될 수 있을 것으로 기대된다. 추후 염증 예방 물질로 적용 될 수 있는 가능성을 높이기 위하여 염증성 작용 기전에 대한 자세한 연구가 필요할 것으로 사료된다. In this study, the anti-inflammatory activity of an extract of the fruiting body of the Tuber himalayense (TH) truffle collected from oak growing areas in Korea was investigated. The extract was not cytotoxic at concentrations below 100 ㎍/mL in an experiment evaluating inflammation inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE₂) production was inhibited by the extract in a concentration-dependent manner. Western blot assay results indicated that the anti-inflammatory activity of TH extract was likely caused by the reduced production of NO and PGE₂ via suppression of induced NO synthase and cyclooxygenase-2 gene expression. In addition, TH extract effectively inhibited the production of interleukin (IL)-1β and IL-6 by macrophages. Thus, TH extract effectively inhibits the overexpression of various inflammatory mediators and could be valuable in formulating anti-inflammatory foods and medicines that target these components.

코로나19 예방행동과 정보추구 의도 연구 : 보호동기이론의 확장과 지지정당의 영향력 검증을 중심으로

김효정(Hyo Jung Kim),홍혜현(Hyehyun Hong) 한국광고홍보학회 2021 한국광고홍보학보 Vol.23 No.2

본 연구는 보호동기이론(protection motivation theory)을 기반으로 개인의 코로나19 관련 정보추구와 예방행동 의도에 영향을 미치는 요인들을 분석하였다. 특히 코로나19 이슈가 정치적 성향에 따라 다르게 인식될 수 있음에 주목하여, 개인의 여당 지지 여부가 미치는 영향을 분석하였다. 코로나19 확산 초기인 2020년 3월 서울 시민 627명을 대상으로 수행한 온라인 설문 결과, 보호동기이론에서 설명하는 바와 같이 코로나19에 대한 위협 평가(심각성 인식, 취약성 인식)와 대처 평가(대처 효능감, 자기 효능감)가 높을수록 예방행동 의도가 높아졌으며, 이때 집권 여당 지지는 위협 평가를 약화시켰던 것으로 나타났다. 더불어, 위협 평가가 높을 때, 그리고 여당을 지지할 때 코로나19 관련 정보추구 의도가 높아짐을 확인하였다. 본 연구를 통해 보호동기이론을 코로나19 상황에 적용하고 개인의 정치적 성향(여당 지지 여부)의 영향을 함께 살펴봄으로써, 보호동기이론을 확장・재검증하고 건강 이슈의 정치화를 논의하는데 기여할 것으로 기대된다. Based on the protection motivation theory (PMT), the current study examined the cognitive factors that influence protective health behaviors related to the COVID-19 pandemic. This study also analyzed how individuals’ political orientation (i.e., individual support for the ruling political party) influences their perceptions and behavioral intentions regarding COVID-19. An online survey of 627 Seoul citizens was conducted in March 2020, which was the early stage of the spread of COVID-19. Consistent with the PMT literature, the results showed that individuals’ threat appraisal (perceived severity and perceived susceptibility) and coping appraisal (response efficacy and self-efficacy) positively influenced their intentions of preventive behaviors against COVID-19. Intentions to seek more information about COVID-19 was positively influenced only by threat appraisal. Regarding the impact of political orientation, it was found that the supporters of the ruling party reported the lower level of threat appraisal and the higher level of coping appraisal, as compared to non-supporters. An individual’s political orientation also influenced their information seeking intention; supporters of the ruling party were more likely to seek more information regarding COVID-19 than non-supporters. Theoretical and practical implications of the findings and directions for future research are discussed.

페이스북 메시지의 발신자 유형과 결론 제시 방식이 커뮤니케이션 효과에 미치는 영향

조정식(Cho, Jungsik),김남경(Kim, Namkyung),홍혜현(Hong, Hyehyun) 한국광고홍보학회 2017 한국광고홍보학보 Vol.19 No.2

최근 SNS가 보편화되고 영향력이 커지면서, 마케팅 커뮤니케이션 채널로 활발히 이용되고 있다. 본 연구에서는 페이스북 메시지의 발신자 유형(유명인 vs. 일반인)과 결론 제시 방식(명시적 vs. 암시적)에 따른 커뮤니케이션 효과를 살펴봄과 동시에, 페이스북 인게이지먼트 수준(저 vs. 중 vs. 고)이 이를 어떻게 조절하는지 분석하였다. 또한 커뮤니케이션 효과를 메시지 태도, 좋아요 클릭 · 공유하기 · 댓글 달기 의도, 온/오프라인 구전 의도 등 다차원적으로 측정하여 그 관계를 탐색하였다. 2 × 2 × 3 실험 설계를 통해 수집한 212명의 데이터를 분석한 결과, 유명인 발신 메시지는 일반인 발신 메시지에 비해 긍정적인 메시지 태도와 좋아요 클릭 의도, 공유하기와 댓글 달기 의도를 이끌었으며, 암시적 결론 메시지는 명시적 결론 메시지에 비해 메시지 태도가 긍정적으로 나타났으나 다른 차원의 효과는 유의하지 않았다. 페이스북 인게이지먼트 수준이 높은 경우, 유명인 메시지에 대한 태도가 특히 긍정적이고 명시적 결론 메시지에 대해서도 오프라인 구전에 관대한 경향이 있는 것으로 나타났다. 논의에서는 페이스북 커뮤니케이션 효과에 대한 학문적 의미를 찾고, 페이스북을 활용하는 마케팅 커뮤니케이션 담당자들을 위한 제언을 제시하였다. In the past few years, SNS (social networking services) have been used as a useful marketing communication tool. The current study aims to examine the effects of the sender type (celebrity vs. non-celebrity) and the message type (explicit vs. implicit) on an array of communication effect indices, including the attitude toward the message, the intention of prompt behavioral reactions (e.g., ‘liking,’ ‘sharing,’ and ‘commenting’), and online/offline word-of-mouth. The study also investigated the moderating role of Facebook engagement. A dataset of 212 participants from an experiment was analyzed. The influence of a celebrity sender and an implicit message was found mainly in terms of one’s reactions such as the attitude toward the Facebook message and the ‘liking’ intention, while rarely in other communication effect indices. In addition, a few interaction effects appeared depending on the level of Facebook engagement. Based on the results, some scholarly insights and practical guidelines are provided.

생물전환 기법을 이용한 토란 뿌리 추출물의 RAW 264.7 세포에 대한 항염증 효과

박태진(Taejin Park),심지한(Ji Han Sim),홍혜현(Hyehyun Hong),한동환(Dong-Hwan Han),김승영(Seung-Young Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.2

Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. We generated Colocasia esculenta L. Schott root extract using Biorenovation technology and tested the anti-inflammatory properties of its Biorenovation product (CEB) in lipopolysaccharide (LPS)-treated RAW264.7 murine macrophages. CEB inhibited the LPS-induced production of pro-inflammatory markers such as nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) in a concentration dependent manner without no noticeable toxicities in RAW 264.7 cells. Then together, our results indicate that CEB could be a new source for the ingredients of cosmetics and pharmaceuticals.

제주산 갯괴불주머니 추출물의 항염증 효과

장성찬(Sungchan Jang),박태진(Taejin Park),김민선(Min Seon Kim),홍혜현(Hyehyun Hong),김미진(Mi Jin Kim),김승영(Seung-Young Kim) 한국생물공학회 2018 KSBB Journal Vol.33 No.4

Several compounds such as libanoridin, heterocarpin, and columbianetin A and crude extract were isolated respectively from Corydalis heterocarpa and Corydalis bungeana and were reported to have anti-tumorigenic and antiinflammatory properties. Corydalis platycarpa (Maxim.) Makino, another Fumariaceae member, have acclimated and prospered in Jeju Island, South Korea. However, the synergistic and inhibition effect of Corydalis platycarpa (Maxim.) Makino crude extract on the expression of LPS-induced inflammatory cytokines and mediators has not been yet examined. Quantitative reverse transcription PCR (qRT-PCR) and immunoblotting were implemented to examine representative inflammatory markers. We found the Corydalis platycarpa (Maxim.) Makino extract (CPE) inhibited dose-dependently the secretion of the five pro-inflammatory indicators NO, PGE2 and TNF-α and suppressed mRNA expressions of iNOS, COX-2, TNF-α, IL-1β, and IL-6. Furthermore CPE exerted its dosedependent anti-inflammatory effect via NF-κB/IκB signaling pathway while down-regulating the protein expression level of iNOS and COX-2 and suppressing the secretion of pro-inflammatory cytokines such as PGE₂ and TNF-α. CPE would be further applied to the development of pharmaceuticals and functional cosmetic ingredients.

Biorenovation 공법을 활용한 광나무 추출물의 미백 효과

심지한(Ji Han Sim),이경미(Kyung-Mi Lee),박태진(Taejin Park),강민성(Min Sung Kang),홍혜현(Hyehyun Hong),김승영(Seung-Young Kim) 한국생물공학회 2021 KSBB Journal Vol.36 No.1

Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant crude-extracts with the potential benefits of reduced cytotoxicities and improved biological activities compared with its reaction substrates. In this study, we examined the whitening effect of Ligustrum japonicum (LJ) and LJ biorenovation extract (LJBR) in α-MSH-stimulated B16F10 melanoma cells. In the non-toxic concentrations of 25, 50, and 100 μg/ml, the inhibitory effect of LJ and LJBR on melanin synthesis and tyrosinase activity was observed. LJBR exhibited a stronger inhibitory effect on melanin synthesis and tyrosinase activity relative to its reaction substrate LJ. In addition, the protein expression level of MITF, TRP-1, TRP-2, and tyrosinase, which are known to play a critical role in melanin synthesis, was measured. As expected, a greater inhibitory effect on melanogenesis was observed in LJBR than in untreated group. These findings strongly suggest that biorenovation can assist the generation of a novel skin-lightening material.