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Biorenovation 공법을 활용한 광나무 추출물의 미백 효과
심지한(Ji Han Sim),이경미(Kyung-Mi Lee),박태진(Taejin Park),강민성(Min Sung Kang),홍혜현(Hyehyun Hong),김승영(Seung-Young Kim) 한국생물공학회 2021 KSBB Journal Vol.36 No.1
Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant crude-extracts with the potential benefits of reduced cytotoxicities and improved biological activities compared with its reaction substrates. In this study, we examined the whitening effect of Ligustrum japonicum (LJ) and LJ biorenovation extract (LJBR) in α-MSH-stimulated B16F10 melanoma cells. In the non-toxic concentrations of 25, 50, and 100 μg/ml, the inhibitory effect of LJ and LJBR on melanin synthesis and tyrosinase activity was observed. LJBR exhibited a stronger inhibitory effect on melanin synthesis and tyrosinase activity relative to its reaction substrate LJ. In addition, the protein expression level of MITF, TRP-1, TRP-2, and tyrosinase, which are known to play a critical role in melanin synthesis, was measured. As expected, a greater inhibitory effect on melanogenesis was observed in LJBR than in untreated group. These findings strongly suggest that biorenovation can assist the generation of a novel skin-lightening material.
Biorenovation 생물 전환 기법을 적용한 주엽나무 잎 추출물이 B16F10 흑색종 세포에 대한 Melanogenesis 관련 유전자 발현에 미치는 영향
김지현(Ji-Hyeon Kim),박태진(Taejin Park),심지한(Ji Han Sim),김승영(Seung-Young Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.4
Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. The purpose of this study was to generated Gleditsia japonica Miquel leaf (GJ) extract using Biorenovation technology and tested the whitening properties of its Biorenovation product (GJBR) in α-melanocyte-stimulating hormone (α-MSH) stimulated B16F10 melanoma cells. As a result of in vitro tyrosinase activity assay, GJBR showed significant whitening effect than GJ. Especially in B16F10 cells, we found that pre-treatment of GJBR efficiently prevented extracellular secretion of melanin with dose-dependent manner. Therefore, it suggests that maybe various components that converted by biorenovation contained in the GJBR affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells. Then together, our results indicate that GJBR could be a new source for the ingredients of cosmetics.
생물전환 기법을 이용한 토란 뿌리 추출물의 RAW 264.7 세포에 대한 항염증 효과
박태진(Taejin Park),심지한(Ji Han Sim),홍혜현(Hyehyun Hong),한동환(Dong-Hwan Han),김승영(Seung-Young Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.2
Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. We generated Colocasia esculenta L. Schott root extract using Biorenovation technology and tested the anti-inflammatory properties of its Biorenovation product (CEB) in lipopolysaccharide (LPS)-treated RAW264.7 murine macrophages. CEB inhibited the LPS-induced production of pro-inflammatory markers such as nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) in a concentration dependent manner without no noticeable toxicities in RAW 264.7 cells. Then together, our results indicate that CEB could be a new source for the ingredients of cosmetics and pharmaceuticals.
흉부 X-ray 검사 시 DEI 값에 따른 적정 관전압에 관한 고찰
채정현(Jeong-hyun, Chae),심지나(Ji-na, Sim),정지상(Ji-sang, Jung),한영수(Yung-soo, Han),진덕은(Duk-eun, Jin),강성호(Sung-ho, Kang),임재식(Jae-sik, Lim) 대한영상의학기술학회 2014 대한영상의학기술학회 논문지 Vol.2014 No.1
목 적 : 흉부 X-ray 검사 시, DR 시스템에 적절한 유효 Detector Exposure Index (DEI) 범위 내의 영상의 Dose Area Product (DAP)값과 유효선량을 비교함으로써, high kVp 사용의 적합성을 영상의 퀄리티나 환자선량 측면에서 평가하기 위함에 있다. 대상 및 방법 : GE definium 8000을 사용하여 흉부팬텀을 이용한 Chest PA 검사를 재현하였다. kVp range는 60~130 kVp로 정하였다. mAs range는 앞의 정한 kVp에 따라 Auto Exposure Control (AEC) 시스템에 의거하여 60kVp에서는 40mAs, 130kVp에서는 2.5mAs로 정하였고 mAs range는 2.5mAs에서, 3.2mAs, 4.0mAs, 5.0mAs, 6.3mAs, 8.0mAs, 10mAs, 12.5mAs, 16mAs, 20mAs, 25mAs, 32mAs, 40mAs로 설정하였다. 위의 kVp 조건에 따라, 각각의 mAs로 총 104번 을 exposure한 뒤, 세브란스 선량관리 시스템으로 전송된 영상의 DEI와 DAP를 획득할 수 있었다. 획득한 영상을 유효한 DEI 범위의 영상들(0.2-0.6 : 정상군)과 DEI 0.2 이하, DEI 0,6 이상인 영상들 을 각각 분류하고 DAP을 이용하여 a PC-Based Monte Carlo Program 2.0 (PCXMC)을 통해 유효 선량을 계산하였다. 각각의 측정한 데이터를 DEI기준, DAP기준, 유효선량의 기준으로 분류한 후, DR 시스템에서 Chest PA 검사 시 적절한 kVp 범위를 분석하였다. 각 그룹의 유의성은 SPSS(version 12.0, SPSS Inc Chicago, IL, USA) 통계 패키지 프로그램을 사용하여 kruskal-wallis test와 사후검정으로 mann -whitney test를 시행하여 검증 하였으며 검증에 사용된 신뢰구 간은 95 %이다. 결 과 : 위의 실험을 통해 얻은 유효한 DEI 범위(0.2-0.6)에 포함된 영상의 평균 kVp는 75.29 kVp 이고 평균 mAs는 8.58 mAs, 평균 DAP는 1.73 mGy·cm², 유효선량은 0.018 mSv 이었다. 유효선 량별로 수집한 군에서 최저의 유효선량인 0.011449 mSv가 측정된 조건은 70kV, 5mAs, DAP 1.06mGy·cm²,이었다. 실측값 DAP에 따른 군에서는 최저 DAP 인 0.885 mGy·cm²에서 조건은 90kV, 2.5mAs, 0.013mSv이 측정되었다. 유의성 검증 결과, 유효 DEI 범위 내의 유효선량에서 유의한 차 이(p<0.05)가 있었고, 전체 DEI 그룹에서 대해서는 0.2 이하 범위와 0.2이상 0.6 이하의 범위, 0.6이상의 범위의 유효선량과 DAP에서 모두 유의한 차이가 있었다.(p<0.05) 결 론 : 각각의 DEI, DAP, 유효선량 기준으로 분류된 데이터를 통해 현재 사용되고 있는 흉부 검사 시 high kVP기법은 환자 피폭적인 측면과 영상의 퀄리티적인 측면에서 재고할 필요성이 있다고 사료된다. Purp os e : This study is to evaluate the suitability of the use of high kVp technique for Chest PA examination in DR system by comparing with Dose Area Product and effective dose of chest images in efficient Detector Exposure Index(DEI) range. Materials and Methods : We set up situation of Chest PA examination with GE definium 8000. kVp range is 60~130 kVp. Range of mAs is 40 mAs at 60kVp, 2.5mAs at 130kVp through Auto Exposure Control system(AEC) in accordance with kVp range we set, then we set up mAs range from 2.5mAs, 3.2mAs, 4.0mAs, 5.0mAs, 6.3mAs, 8.0mAs, 10mAs, 12.5mAs, 16mAs, 20mAs, 25mAs, 32mAs to 40mAs. We exposured 104 times as the kVp condition we set, then all images are sent to the Severance Dose management system which we can acquire DEI and DAP data. We classified acquired images into several DEI groups–under 0.2, between 0.2 and 0.6, over 0.6, then calculated Effective Dose with DAP by using a PC-Based Monte Carlo Program 2.0(PCXMC). After classifying acquired data into 3 groups according to DEI, DAP and effective dose, we analyzed suitable kVp range for Chest PA examination in DR system. SPSS(version 12.0, SPSS Inc Chicago, IL, USA) is used for statistical significance, which is Kruskal-Wallis test and Mann-Whitney test to each group. Confidence interval is 95%. Res ult : Average kVp of image in efficient DEI range from the experiment is 75.29 kVp, mAs is 8.58 mAs, DAP is 1.73 mGy·cm² and effective dose is 0.018 mSv. The condition which 0.011449 mSv is acquired as the lowest effective dose is 70 kVp, 5mAs, DAP 1.06mGy·cm² in groups classified with effective dose. Another condition which 0.885mGy·cm² is acquired as the lowest DAP is 90kV, 2.5mAs, and Its effective dose is 0.013mSv. As a result of statistic significance test, there is significance difference in effective dose of the efficient DEI range group(p<0.05) and significance difference is found in whole DEI groups of under 0.2, between 0.2 and 0.6, over 0.6(p<0.05) Conc lus ion : According to each classified data with DEI, DAP, Effective dose, high kVp technique for Chest PA examination which is currently used is need to be reconsidered in patient exposure and image quality.
B16F10 Melanoma 세포에서 홍지네 고사리 추출물의 Tyrosinase 활성과 Melanin 생성에 미치는 영향
구연수(Yeon-Su Koo),박태진(Taejin Park),심지한(Ji Han Sim),김민선(Min-Seon Kim),김승영(Seung-Young Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.3
In this study, we investigated the effect of Dryopteris erythrosora extract on anti-oxidation and tyrosinase activity and melanin production as natural products of functional cosmetics. Melanin contents of B16F10 melanoma cells were decreased by 10%, 21%, and 24% in a dose-dependent manner, upon Dryopteris erythrosora extract treatment at 50, 100, and 200 ㎍/mL, respectively. Concentration of 200 ㎍/mL of Dryopteris erythrosora extract especially induced greater decreases in tyrosinase activity. As a result, Dryopteris erythrosora extract showed a 70% reduction in concentration of 200 ㎍/mL. Dryopteris erythrosora extract suppressed expression of tyrosinase, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16F10 melanoma cells. Concentration of 200 μg/mL of Dryopteris erythrosora extract especially induced greater decreases in tyosidase activity and melanogenic enzyme protein expressions. This results indicate that Dryopteris erythrosora extract inhibits melanin synthesis and tyrosinase activity, and Dryopteris erythrosora extract may be an ideal candidate as a skin whitening agent.