양.한방협진제도에 대한 직장인들의 태도

구제길,노홍인,홍선미,강인숙,이영호,한동운,Goo, Je-Gil,No, Hong-In,Hong, Sun-Mee,Kang, In-Sook,Lee, Young-Ho,Han, Dong-Woon 대한예방한의학회 2009 대한예방한의학회지 Vol.13 No.2

Background and Objectives: The purpose of the study was to explore the attitude of workers toward a system of collaborative hospital practice between western and traditional Korean medicine, to identify factors influencing this attitude, and discuss the reasons socioeconomic groups' differences. Method: The data were collected with a questionnaire for this study from 14 April 2009 to 1 May 2009. Data were analyzed mainly via non-parametric statistics and logistic regressions utilising SPSS 17.0 (Statistical Package for the Social Sciences) to determine the workers' attitude about the hospital system and to predict factors contributing to positive attitudes. Results: A total of 1,260 workers working for large factories in Gwangju Metropolitan City. Findings confirmed that more than 40% of the workers show interest in the system and about 44% of the workers also express positive attitudes. Factors found to influence the workers' response included marital status, income level, health status, experience in complementary medicine services, the number of health care facilities' visit. Conclusions: The prospects to establish the system of collaborative hospital practice as reflected by the workers' view about the Korean health care service delivery system. Their attitudes toward the system differed among socioeconomic groups. Government and health care providers should identify the socioeconomic subgroups' demands and opinions in order to find and develop measures of integrating western and traditional Korean medicine in health care facilities.

누에로부터 핵다각체병 바이러스 방어관련 유전자 정보 분석

구태원 ( Tae Won Goo ),홍선미 ( Sun Mee Hong ),김성완 ( Sung Wan Kim ),최광호 ( Kwang Ho Choi ),김성렬 ( Seong Ryul Kim ),박승원 ( Seung Won Park ),강석우 ( Seok Woo Kang ),윤은영 ( Eun Young Yun ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2

Silkworm larvae often suffer from viral infections causing heavy losses to the economy of silk industry. Insects exhibit both humoral and cellular immune responses that are effective against various pathohens like bacteria, fungi, protozoa, etc., but no insect immune responses is effective against viral infection. To obtain genes related to insect antiviral immunity from Bombyx mori, the cDNA library was constructed from B. mori nucleopolyhedrovirus (BmNPV)-infected B. mori. From the cDNA library, we selected 411 differentially expressed clones, and the 5` ends of the inserts were sequenced to generate ESTs. In this work, 135 unigenes were generated after the assembly of 411 differentially expressed clones ESTs. Of these 135 unigenes, we selected 109 antiviral response-related candidates except 26 clones that high similarity with genes derived from BmNPV. Among 109 unigenes, a total of 80% had significant matches to genes from other organisms in the database, wheres 20% of the unigenes had not matched in the database. Functional groups of these sequences with matches in database were constructed according to their putative biological function. Three largest categories were control of cellular oraganization (52%), metabolism (20%), and protein fate (10%). The genetic information reported in this study will provide more information about antiviral-related genes in silkworms.

해방풍 유산균 발효물의 항균 및 항산화 증진 효과

조현솔(Hyun Sol Jo),홍선미(Sun Mee Hong) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.2

본 연구는 동해안 해수에서 분리된 L. plantarum (Lp)과 P. acidilactici (Pa)를 이용한 해방풍 발효를 통해, GABA 증량과 항산화, 항균 및 멜라닌 억제 효능을 확인하였다. 해방풍 잎과 뿌리 추출물의 Lp와 Pa의 공발효(GLE_LpPa, GRE_LpPa)에서 유산균은 각각 1.0E+11과 1.5E+12 cfu/mL의 성장을 나타냈고, 잎과 뿌리의 GABA 합성이 GLE_LpPa는 16 %p, GRE_LpPa는 167 %p의 증량이 확인되었다. 해방풍 잎 발효물의 DPPH 라디칼 소거능은 GLE_Lp 75.6%, GLE_Pa 79.2%로 높은 항산화 활성을 보였으나, 뿌리 발효물인 GRE_Lp 20.9%, GRE_Pa 23.2%로 조금 낮았다. 또한, 잎과 뿌리 발효물의 B16-F10 세포 독성 실험은 1,000 ug/mL 농도의 72시간까지 세포가 안정적으로 성장하는 것으로 나타났다. 또한 각 배양물은 B16-F10 세포에서 멜라닌 억제 효능이 확인 되었으며, 특히 유산균 Lp에 의한 GLE_Lp와 GRE_Lp는 각 71.3%와 68.6%로 Pa 발효보다 높은 멜라닌 억제 기능이 확인되었다. 또한 뿌리 공배양물인 GRE_LpPa는 추출물인 GRE보다 치아우식균 2.2배, 충치균 2.5배, 여드름균 1.25배, 특히 비듬균에 있어서는 12배의 항균 활성 증가가 확인되었다. 이들 결과는 다른 영양성분 첨가 없이 Lp와 Pa가 접종된 해방풍 발효는 GABA 증량이 가능하고, GABA가 포함된 해방풍 발효물은 항산화, 항균과 멜라닌 억제 효능과 세포 안정성이 확인되어 식품 및 화장품 산업의 유효 소재로의 활용 가치가 있을 것으로 사료된다. In this study, the effects of Lactobacillus plantarum (Lp) and Pediococcus acidilacitici (Pa) based fermentation of Glehnia littoralis extracts on GABA levels and antioxidative, antibacterial, and anti-melanin effects were investigated. Lp and Pa co-culture of G. littoralis leaf and root extracts (GLE_LpPa, GRE_LpPa) led to bacterial growth rates of 1.0E+11 and 1.5E+12 cfu/mL, respectively. After 48 h of fermentation, the amounts of MSG and GABA produced were enhanced. In particular, GABA levels in GLE_LpPa and GLE_LpPa were found to have increased by 16% and 167%, respectively. Fermentation of GLE_Lp and GLE_Pa resulted in DPPH activity increases of 75.6% and 79.2%, indicating that both fermented extracts had antioxidative activity. B16F10 cell assays showed GLE_Lp and GRE_Lp inhibited melanin levels by 71.3% and 68.6%, respectively. Furthermore, GRE_LpPa showed enhanced activities against bacterial species that cause dental caries (2.2-fold for Streptococcus mutans, 2.5-fold for Streptococcus sobrinus ) and acne (1.25-fold for Propionibacterium acnes ). Of note was a 12-fold increase in activity against the dandruff bacterium Malassezia furfur . In conclusion, the study shows that the antioxidative, antibacterial, and melanin-inhibiting effects and GABA levels in G. littoralis extracts are enhanced by fermentation, in the absence of additional nutrients. These results support the use of fermented Glehnia littoralis extracts as active ingredients in the food and cosmetics industries.

누에 short neuropeptide F receptor (BsNPF-R)의 cDNA cloning

신효정(Hyojung Shin),권기상(Kisang Kwon),홍선미(Sun Mee Hong),김홍근(Hong Geun Kim),박관호(Kwan-Ho Park),최지영(Ji-Young Choi),김승환(Seung-Whan Kim),유권(Kweon Yu),권오유(O-Yu Kwon) 한국생명과학회 2016 생명과학회지 Vol.26 No.6

누에(Bombyx mori)에서 short neuropeptide F (sNPF) receptor를 encoding하고 있는 cDNA를 cloning하여서 BsNPF-R라고 이름을 붙였다. BsNPF-R는 이미 보고된 sNPF-R들과 아미노산 수준에서 사람(36%), 쥐(34%), 제브라피쉬(35%), 초파리(51%)와 상동성을 보였다. BsNPF-R는 계산적으로 분자량이 42,731 Da이고 원형질막을 관통하는 단백질이다. BsNPF-R 유전자발현은 중장, 후견사선, 말피기관, 정소에서 강한 반면 지방체, 혈세포, 난소에서 약하게 발현하였다. 그리고 합성된 sNPF에 의해서도 BsNPF-R의 유전자발현이 조절되었다. It has already been reported that short neuropeptide F (sNPF) stimulates feeding behaviors in a wide variety of insect species. In the present study, we cloned cDNA, encoding a sNPF receptor homologue from a silkworm, Bombyx mori, named BsNPF-R. The amino acid sequence of BsNPF-R was compared with those of sNPF-R thus far reported, which is shared with humans (36%), mice (34%), zebrafish (35%), and fruit flies (51%), respectively. A BsNPF-R protein"s mass was theoretically estimated to be 42,731 Da and it is a putative plasma membrane-penetrating protein. The mRNA expression of BsNPF-R was tested; the results showed that a strong expression was detected at the midgut, post-silk gland, Malpighian, and testis; however, a weak expression was at the fat body, hemocyte, and ovary. In addition, the synthesized sNPF of a silkworm regulated the BsNPF-R mRNA expression through the cell-based functional analysis.

유산균 발효에 의한 흰점박이꽃무지 염장소스 제조 및 특성

조현솔(Hyun Sol Jo),김태하(Tae Ha Kim),홍선미(Sun Mee Hong) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.9

본 연구는 식용곤충 기반의 소스 개발을 위해, 흰점박이꽃무지(꽃뱅이; Protaetia brevitarsis, Pb)를 40 % 천일염(solar salt; ss)과 정제염(refined salt; rs)으로 염장하여 간장 형태의 조미료를 제조하였다. 또한, 항균력을 지닌 유산균 L. plantarum (Lp)과 W. paramesenteroides (Wp) 접종에 의한 유해 미생물 발생 억제와 3개월 속성발효에 따른 그 기능을 제시하였다. 40 % 농도의 천일염과 정제염으로 염장하고 3개월간 숙성된 흰점박이꽃무지 염장소스(Pb sauce; ssPB_Lp/Wp, rsPB_Lp/Wp; 꽃뱅이장) 내의 미생물을 16s DNA로 분석한 결과, 두 종류 소스에서 투여된 Lp만 확인되었고 기타 미생물은 확인되지 않았다. Lp와 같이 투여된 Wp는 기타 미생물 발생 억제 기능 후 고염 생존이 어려웠다. 두 종류 꽃뱅이장의 평균 pH, 당도, 염도는 pH 6.1, 32 brix, 27.8 %로 시판 제품과 비교한 결과, 유사범위에 속하였으며 유산균 숙성에 따라 GABA 성분이 증가하고 유리아미노산 형태인 프롤린(491.02 ㎎/100 g), 글리신(198.86 ㎎/100 g), 알라닌(140.67 ㎎/100 g), 글루탐산(71.87 ㎎/100 g) 외의 14종 아미노산이 확인되었다. 또한, 타르색소와 안식향, 팔라옥시안식향산, 소브산 등의 식품첨가물도 불검출 또는 기준 수치 이하로 검출되었다. 다음으로 효능분석에서 항산화 능력 검정(DPPH) 결과, 천일염 꽃뱅이장(ssPB_Lp/Wp)이 69.4 %로 높은 항산화 효과를 나타내었으며, 3T3-L1 세포 독성평가 안전성 및 지방축적 감소 효과가 있음도 확인되었다. 본 연구 결과는 흰점박이꽃무지 외의 식용곤충을 활용한 천연 식용곤충 소스류 개발에 대한 가능성과 유효 제품 개발에 도움이 될 것으로 사료된다. The purpose of this study was to develop an insect-based sauce fermented by lactic acid bacteria (Lactobacillus plantarum [Lp] and Weissella paramesenteroides [Wp]). This study suggested salting as an accelerated method for fermenting the edible insect Protaetia brevitarsis (Pb). Brine containing Pb and 40 % solar or refined salt was inoculated with Lp and Wp to inhibit harmful bacterial growth and facilitate fermentation (ssPb_Lp/Wp and rsPb_Lp/Wp). Three months after inoculation, 16s rDNA sequencing identified Lp as the only microbe in the Pb sauce regardless of the salt used. Although Wp withstood the growth-inhibiting effects of other microbes, it did not survive in brine. The ssPb_ and rsPb_Lp/Wp characteristics (average pH 6.1, sugar content 32 Brix, and salinity 27.8 %) resembled those of commercial fish sauce products. It contained proline (491.02 ㎎/100 g), and 17 other amino acids. Preservative (e.g., benzoin, 4-hydroxybenzoic acid, sorbic acid, and tar pigment) levels complied with the Korean Food Standards Codex. The Lp/Wp inoculation increased gamma-aminobutyric acid levels and reduced adipose accumulation. The antioxidant activity was highest (69.4 %) in ssPb_Lp/Wp. Our study demonstrated the potential of Pb- and/or other insect-based sauces and the validity of applying our method to the development of such products.

사료내 갈색거저리 유산균 발효물 첨가의 돌가자미 치어 성장 효과

조현솔(Hyun Sol Jo),박무억(Moo Eog Park),홍선미(Sun Mee Hong) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.4

본 연구는 치어 돌가자미(Kareius bicoloratus ) 성장에 도움을 줄 수 있는 사료첨가물 개발을 위해 갈색거저리 유충 분말배지(5 %)에 어병 항균 기능을 가지는 유산균을 발효 한 후, 사료첨가물로써의 효능을 실험하였다. 어류 병원체(비브리오균, 연쇄상구균)에 대한 항균기능을 가진 유산균(LAB; L. plantarum , W. paramesenteroides, P. acidilactici )으로 발효한 갈색거저리 추출 발효물의 양어 성장 실증실험을 위해 총 600 마리의 치어 돌가자미(약 5∼6 g)를 공시하여 100마리씩 수조 6개에 임의 배치한 후 각 3수조씩 대조군과 실험군으로 분류하여 6주간 평균 전장과 체중을 측정하였다. 6주 후에 대조군의 전장 평균은 8.26 ㎝, 실험군은 8.42 ㎝이며, 대조군의 체중 평균은 19.71 g, 실험군은 20.28 g으로 HeTm_LpWpPa 첨가에 있어 성장효과가 증대됨을 확인하였다. 6주간의 증체율은 대조군 223.26 %, 실험군 248.76 %로 HeTm_LpWpPa 첨가군이 25.5 %p 성장효과가 높고, 6주 동안의 일간성장률은 대조군 2.79 %/일, 실험군 2.98 %/일로 실험군이 0.19 %p 더 높았다. 또한 평균 체중 이하의 개체 수에 있어서도 3주째에는 대조군 27마리(9 %), 실험군 9마리(3 %)이고, 6주째에는 대조군 17마리(5.7 %), 실험군 3마리(1 %)로 실험군에서는 평균 체중 이하가 1 %로 균일한 성장 효과가 있었다. 이들 결과는 동애등에 유충 추출 유산균 발효물(HeTm_LpWpPa) 사료 첨가물의 10 % 내외까지 사용은 돌가자미를 포함하는 양어 치어의 성장 촉진에 유효할 것을 시사한다. In this study, we aimed to develop a feed additive that can promote the growth of stone flounder (Kareius bicoloratus ). To this end, we fermented mealworm medium (5%, w/v) with lactic acid bacteria (LAB) with antibacterial function (HeTm_LpWpPa) and evaluated its efficacy as a feed additive. Fish (approximately 5-6 g) were reared in six completely recirculating tanks (100 fish/tank) for 6 weeks. The total length of fish with and without HeTm_LpWpPa supplementation was 8.26 and 8.42 ㎝ and body weight was 19.71 and 20.28 g, respectively, at 6 weeks. The weight gain of the control and experimental groups was 223.26 % and 248.76 %, respectively. The specific growth rate at 6 weeks was 2.79 % (control) and 2.98% (experimental), and the growth rate of the experimental group was slightly higher (0.19 %) than that of the control group. At the end of the experiment (i.e. 6 weeks), the number of fish below the mean weight in the experimental group was 3 (1 %) and that in the control group was 17 (5.7 %). These results indicate that feed supplementation of 10 % HeTm_LpWpPa could be utilized to promote the growth of flounder in farming.

확장성 심근증에 있어서 기능적 영상에 의한 심실벽 운동의 평가

이방헌(Bang Hun Lee),이오영(Oh Young Lee),김정현(Jeong Hyun Kim),임헌길(Heon Kil Lim),이정균(Chung Kyun Lee),조석신(Suhk Shin Cho),김주형(Ju Hyung Kim),강영희(Yeong Hee Kang),홍선미(Sun Mee Hong) 대한내과학회 1991 대한내과학회지 Vol.40 No.2

N/A To evaluate the regional wall motion of left and right ventricles in dilated cardiomyopathy (DCM), the authors carried out functional studies (phase image histogram, and ejection fraction image) by computer-assisted application of radionuclide ventriculography (RVG) and compared functional images with regional ejection fraction by RVG in 17 patients with DCM and 20 normal controls. The results are as follows: 1) Compared with normal controls, regional ejection fraction of left ventricle and ejection fraction of right ventricle were significantly reduced in DCM (all p<0.001). 2) The standard deviation of phase angle in histogram of left ventricle in DCM was significantly greater than normal group. 3) In DCM there was agreement between phase image and regional ejection fraction of left and right ventricle by RVG as to the presence of asyergy in 84.3%, and 94.1% respectively. 4) In DCM, asynergy was noted in all three regions of left ventricle and there was agreement between ejection fraction image and regional ejection fraction as to the presence and localization of asynergy in 94.1% and 98.0% respectively. These results suggest that the advantage of phase image is to quantify the degree of abnormal wall motion and ejection fraction image is superior to phase image in the differentiation between normal and abnormal wall motion and in the localization of asynergy in dilated cardiomyopathy.

임상 사회사업의 발전과 과제

홍선미 한국사회복지연구회(서울대학교 사회복지연구소) 1999 사회복지연구 Vol.14 No.1

The clinical social work is a practice speciality of the social work profession. In spite of struggle to advance their status as professional social workers, at present, clinical social workers face many dilemmas in defining their professional base. This article reviews definition of clinical social work and numerous state-of-the-profession issues. Through an examination of a series of definitions of clinical social work, this article reveals the problematic nature of clinical social work, in relation to its concept, boundary and function. A historical review of the growth of the profession identifies internal and external influences in shaping the nature of clinical social work. In pursuit of professional development, clinical social work confronts with several dilemmas related with identity and professional status of the profession. Due to its knowledge base borrowed from psychology, clinical social work is frequently blamed for its irrelevant professional base. With an insufficient effort to share the common base of social work, clinical social work cannot share the value and missions of social work. In order to integrate clinical approaches to social work practice, two practice is are proposed; the clinical case management model and the broker case management model Several practical strategies for the new direction for future professional development are also suggested.

Seltzer Model에서 인삼과 morphine의 공동투여가 통각행동에 미치는 영향

홍선미,강영님,김광진 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The aim of this study was to compare the effects of the ginseng total saponin(100㎎/㎏, i.p., GTS), morphine(3㎎/㎏, i.v.) and the co-administration of the GTS and morphine at same time after the unilateral tight ligation of about half of the sciatic nerve in rats. This tight ligation evoked a markedly different responses of the neuropathic pain in both injured left sides and non-injured right side, which is representing the mirror effect in spite of the time courses of the various stimili; mechanical, chemical and cold, but exception of spontaneous. The maximal responses of each stimulus showed neuropathic pain evoked at the seventh day, and the values of the response were used as a indicater of the nerve injury group for drugs; GTS alone, morphine alone, and the co-administration of GTS and morphine at same time. The effect of the GTS was continued for approximate 48 hours and morphine for 2 hours, respectively. The co-administration of the GTS and morphine at same time, however, showed the no considerable antinociceptive effect at first injection, but the consideralble difference after injection of 24 hours of 48 hours. And also these responses showed the mirror-like response after the drug treatments. These results suggest that partially tight ligation evoked the neuropathic pain in the various stimuli showing the mirror-like effect, and the actions between GTS and morphine has the antagonistic relationship with each other, and the action mechanism is partly involved the non-opioid and opioid mechanisms.

급성 담낭염에 대한 내과적 치료의 역할

강성원,조한래,한설해,박현주,정인성,홍선미,김성걸,이헌영 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

In 58 patients who were admitted to department of internal medicine, ChungNam national university hospital from January 1989 to March, 1993. We evaluated for medical treatments and the following results were obtained. 1) The ratio of male and female was 1 : 1 and mean age of the patients was 57.4 years. 2) At arrival on hospital, the most common clinical symptom was pain in right upper quadrant and epigastrium(93.1%), the remainders were fever and chill(79.3%), nausea(51.7%) and vomiting(36.2 %) in order of frequency. 3) The most common physical findings was tenderness on the right upper quadrant and epaigastrium(98%), the remainders were jaundice(50%) and palpable gall bladder(13.8%) in order of freguency. 4) In laboratory examination, elevated levels of ESR(82.8%), leukocytosis(67.2%), elevated levels of AST(62.0%), ALT(50.0%), serum total bilirubin(51.7%) and alkaline phosphatase (43.1%) were noted. 5) The mean period of NPO was 3.9 days and the mean hospital period was 13.5 days. 6) The rates of combined complications in patients with acute cholecystitis were 17.2%; these are gall bladder empyema(5.2%), hydrops(5.2%), perforation(3.4%), sepsis(3.4%) and intestinal obstruction (1.7%). 7) In 96.6 percent of 58 cases, the symptoms of acute cholecystitis were improved by conservative management. 8) The mean follow-up period was 25.5 months and the recurrence rate was 10% during this period.