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A stydy on The Biological Control of Sciarid Fly(Lycoriella sp.) Using Bacillus thuringiensis
최광호,박현철,박현우,진병래,강석권,손흥대,Choi, Kwang-Ho,Park, Hyean-Cheal,Park, Hyun-Woo,Jin, Byung-Rae,Kang, Seok-Kwon,Sohn, Hung-Dae 한국생명과학회 1996 생명과학회지 Vol.6 No.4
Thirteen subspecies of Bacillus thuringiensis including B. t. israelensis, B. t. morrisoni PG-14 and B.t. darmstadoemsos known to be toxic to dipteran insects were treated on the mushroom (Flammulina velutipes) compost to estimate the biological control effect of a sciarid fly, Lycoriella sp. According to the results, it was found that there were no significant effects of the tested strains of B, thuringiensis on the control of Lycoriella sp. For further confirmation, larval gut juice of Lycoriella sp. and trypsin were respectively treated into the parasporal crystal proteins of three subspecies of B. t. israelensis, B. t. morrisoni PG-14, and B. t. darmstadiensis. The proteins were separated by SDS-PAGE. According to the results, the major parasporal crystal proteins were respectively produced by B. t. morrisoni as the amount of 52 kd, B. y. israelensis as 37kd and B. t. darmstadiensis as 39kd, but the activity of these proteins could not be unfortunately confirmed in this study.
철골 보 - 철근콘크리트 기둥 접합부의 휨 및 전단 저항성능에 관한 연구
최광호,Choi, Kwang Ho 한국강구조학회 2000 韓國鋼構造學會 論文集 Vol.12 No.4
철골 보와 철근콘크리트 기둥으로 구성된 복합 시스템은 철골과 철근콘크리트 간에 재료적인 단점을 보완함으로써 구조 효율성과 시공 생산성을 높이는 이점을 가지고 있다. 이 연구는 철골 보에 현장 타설 철근콘크리트 기둥을 간편하게 접합시킬 수 있는 복합구조 시스템을 개발하고, 그 시스템의 휨 및 전단 저항성능을 시험을 통하여 규명하는 것을 목적으로 하고 있다. The composite system, which is consisted of the steel girder and reinforced concrete column has some advantages in the structural efficiency and the construction productivity by complementing the shortcomings between the two materials. This research is aimed at the development of the composite beam-column connection system by which the steel beam can be connected to the R/C column with smooth stress transfer. And, to ensure safety of this system, the tests of moment and shear resisting performance have been carried out for actual size specimen. From the test, the connection system has been preyed to take good resistance and stress transfer between steel girder and reinforced concrete column.
최광호,권대현,Choi, Kwang-Ho,Kwon, Dae-Hyeon 해양환경안전학회 2011 해양환경안전학회지 Vol.17 No.4
We investigated the biological characteristics of the squid, Todarodes pacificus Steenstrup through the samples caught monthly in the Yellow Sea($32^{\circ}$ 30'N, $122^{\circ}E-37^{\circ}$ 30'N, $127^{\circ}E$) from 1985 to 1987. Monthly mantle length frequency were separated into Autumn spawning group and winter spawning group with Tanaka's method(1956) to determine the main group of the squids caught in the Yellow Sea. The ratio of winter spawning group was higher than that of autumn spawning group. Monthly gonadosomatic index of females increased on July in 1985, on October in 1986, and October in 1987 and decreased later in each year. That of males increased on June in 1985, on July in 1986, and June in 1987. The length of 50% group maturity of female was 20.7 cm mantle length(ML) in 1985, 17.9 cm ML in 1986, and 17.2 cm ML in 1987. The ratio of female was higher than that of male in the group over 24 cm ML, and the ratio of male was higher than that of female in the group under 24 cm ML. We concluded that the length of 50% group maturity of squids was affected by the density of the population status. 1985년부터 1987년까지 황해 $32^{\circ}$ 30'N, $122^{\circ}E-37^{\circ}$ 30'N, $127^{\circ}E$ 해역에서 오징어채낚기어업으로 어획된 살오징어($Todarodes$ $Pacificu$s Steenstrup)를 매월 1회 채집하여 생물학적 특성치를 조사하였다. 황해에서 어획된 살오징어의 주 발생군을 판단하기 위해 Tanaka(1956)의 방법을 사용하여 가을과 겨울발생군으로 분리하였다. 겨울발생군의 비율이 가을발생군의 비율보다 높았다. 생식소 숙도지수(GSI) 월변화에서 암컷은 1985년에는 7월에, 1986년에는 10월에, 1987년에는 8월에 최고값을 나타낸 후 감소하였다. 수컷은 1985년에는 6월에, 1986년에는 7월에, 1987년에는 6월에 최고값을 보였다. 암컷의 50% 군성숙체장를 분석한 결과 1985년에는 외투장(ML) 20.7 cm, 1986년에는 17.9 cm, 1987년에는 17.2 cm로 나타났다. 암수비율은 ML 24 cm 이상 체급에서는 암컷의 비율이 높았으며, 24 cm 미만 체급에서는 수컷의 비율이 높았다. 살오징어의 군성숙체장은 개체군 상태에 의해 영향을 받는 것으로 판단된다.
최광호,Choi, Kwang-Ho 한국환경영향평가학회 2011 환경영향평가 Vol.20 No.2
Causing by green house gas emission, global warming is being accelerated significantly. This global warming cause world climate to change quiet different than before and we call this phenomenon is Climate Change. Environmental Impact Assessment being implemented in Korea is to prevent predicted environmental impacts from deteriorating within the domestic information and situation. As the climate change is getting severe, new meteorological records can be occurred which is exceeded existing statistical data. According to KMA(Korea Meteorological Administration) data, maximum value of precipitation and temperature in many regions changed with new data within last decade. And these events accompanied with landslides and flooding, and these also affected on water quality in rivers and lakes. According to impacts by climate change, disasters and accidents from heavy rain are the most apprehensive parts. And water pollution caused by overflowed non-point sources during heavy rain fall, fugitive dust caused by long-term drought, and sea level rise and Tsunami may affect on seaside industrial complex should be worth consideration. In this review, necessity of mutual consideration with influences of climate change was considered adding on existing guideline.
최광호 ( Kwang Ho Choi ),김성렬 ( Seong Ryul Kim ),강석우 ( Seok Woo Kang ),박옥란 ( Yulan Piao ),김성완 ( Sung Wan Kim ),김기영 ( Kee Young Kim ) 한국잠사학회 2015 한국잠사곤충학회지 Vol.53 No.2
본 실험에서는 누에 피브로인 L-chain 유전자의 인트론 영역을 사용하여 우리나라에서 보존하고 있는 누에 계통중 원산지별 누에 계통 8개를 대상으로 계통 간 유전자 다형성을 비교하였다. 실험 결과, 실험에 사용한 누에 계통에 따라서 동일한 계통 내 모든 개체에서 FibL1과 FibL2의 유전자다형성 조합이 일정하게 유지되어 있었으며, 계통 간에는 FibL1과 FibL2의 유전자 다형성 패턴 조합이 다소 상이한 것으로 확인되었다. 따라서, 프라이머 FibL1 와 FibL2에 의한 피브로인 L-chain 유전자 다형성 염기서열 정보를 활용한다면 제한된 영역에서 누에 계통 구분을 위한 지표로 활용할 수 있을 것으로 사료된다. We have previously characterized the complete fibroin light chain gene from one of the silkworm race Baegokjam (Bombyx mori) and found two variable regions (FibL1, intron 2 ~ 3; FibL2, intron 6) with the primer sets designed to cover these variable regions. In this study, we tested the utility of these variable regions as genetic markers for classifying silkworm races. For the purpose, Europian races (Q, PK), Chinese races (C26, C31), Japanese races (N15, N9), and tropical races (SA2, SA5) were used in this experiment. The polymorphism of the FibL1 and FibL2 were divided into two and three types, respectively. The combination of the FibL1 and FibL2 polymorphisms were constant within the same races. The result suggest that the primer sets designed from two variable regions of fibroin light chain gene may be useful as the genetic markers for silkworm races.
한국 연근해 오징어의 어황 특성 1 . 해양환경의 변동에 따른 오징어의 분포
최광호(Kwang Ho Choi),황선도(Sun Do Hwang),김주일(Ju Il Kim) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.4
Annual variations in main fishing grounds of common squid with different angling and their fishing conditions in Korean waters were studied by analyzing catch and water temperature data. The main fishing grounds of squid angling fishery started to moved to the north in April and to the south in September in the East Sea of Korea. The catch of squid was related to the direction of thermal fronts. The catch was high when the thermal front was formed in the east-west direction, while there was low catch when the thermal front was formed in the south-north direction which runs parallel to the coast.