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玄鎬燮 서울大學校 保健大學院 1971 公衆保健雜誌 Vol.8 No.1
For the purpose of analysing the issues involved in the interrelationships between health services for mothers and children and government sponsored family planning programs and assessing the feasibility of an integrated administration of these closely related services, the Author conducted an opinion survey towards family planning senior field workers from both the urban and rural health centers. Pre-coded questionnaire forms were distributed to respondents in classrooms held for an inservice training of the field workers, from 7 through 21 October 1970. The conclusions obtained from this study can be outlined as follows; 1. Under stitustions prevailing in Korea, it is desirable that MCH service and family planning service be operated under one umbrella at all administrative levels, because; a. the target populations of the two services are practically idential, b. medical and health backgrounds as well as facts as basis of the two services are almost common to each other, c. basic training of the field persommel to be mobilized for these two services should involve technical subject-matter much in common in nature, and d. funds for each of the two services are much limited in scale. 2. Both the MCH and family planning services, in terms of their program contents, still are much apart from the ideal types theoretically constructed. 3. Program contents of both MCH and family planning services in rural areas lack a balance compared with those for the urban area. 4. Cooperation and coodination between the two services are deficient both in rural and urban areas, mainly for organizational and budgetary segregation. 5. A greet mejority of family planning field workers are in support of an integrated administration of MCH and family planning services. 6. A low level of recognition as to the practical know-how of an integrated MCH and family planning services has been assessed with family planning field workers. Based on the above findings, it is recommended that 1. A study of much larger scope and higher intensity on the subject of integration of MMCH and family planning services is desirable. 2. A study should be conducted on the practical procedure of evaluating these two services as the integral parts of one comprehensive health services oriented towards mothers and children.
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각종 요인이 물속의 Diastase 활성에 미치는 영향
윤복상,현호섭,백남원,Yoon, Bock-Sang,Hyun, Ho-Sup,Paik, Nam-Won 대한예방의학회 1974 Journal of Preventive Medicine and Public Health Vol.7 No.1
Many factors exert an influence on enzyme activity and thus on the rate of reactions that they catalyse. The most important of these factors are pH, temperature, substrate concentration, and the concentration of some inhibitors present. A solution of the enzyme diastase, which breaks down molecules of the polysaccharide starch to the disaccharide maltose by hydrolysis, was provided. Activity of this enzyme was measured by the rate at which starch was removed from the reaction mixture. These experiments were designed to study this reaction rate under varying conditions and the following results were obtained. 1. The range of optimum pH for this enzyme at room temperature was 4.0-7.0 and the optimum pH was 5.0. 2. The range of optimum temperatures for this enzyme at pH 7.0 was $30^{\circ}C-50^{\circ}C$ and the optimum temperature was $40^{\circ}C$. 3. The relationship between the enzyme activity and substrate concentration could be expressed by the Michaelis-Menten equation. The limiting velocity of this enzyme at room temperature and pH 7.0 was $415{\mu}g$ starch removed/ml of reaction mixture/min and $K_m$, Michaelis constant, was $343{\mu}g/ml$. 4. Inhibitors NaCl and $HgCl_2$ blocked this enzyme activity completely at 1% and 0.01% respectively. 위생공학에서 하수나 공장폐수를 처리할 때 이용하는 생물학적 처리과정은 미생물에서 생성되는 효소에 의하여 이루어 진다. 본 연구에서는 diastase의 활성에 대하여 수소이온 농도, 온도, 기질 농도 및 방해물질 등 여러가지 요인이 미치는 영향을 실험적으로 관찰하여 다음과 같은 결론을 얻었다. (1) diastase의 활성도는 pH $4.0{\sim}7.0$의 범위가 적당하였고 최적치는 pH 5.0이었다. (2) diastase의 활성도는 $30^{\circ}C{\sim}50^{\circ}C$ 높았으며 최적온도는 $40^{\circ}C$였다. (3) 기질농도의 영향을 보면 녹말농도 $750{\mu}g/ml$까지는 diastase의 활성도가 급격히 증가하였고, 그 이후에는 한정속도를 나타냈다. Michaelis 방정식을 적용시켜 보면 한정속도는 $415{\mu}g/ml$ starch removed/ml of reaction mixture/min였고 Michaelis 상수는 $340{\mu}g/ml$였다. (4) 방해물질의 영향을 보면 NaCl 농도 1.0%, $HgCl_2$ 농도 0.001%에서 diastase의 활성은 완전히 억제되었다.
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