실험적 녹농균각막염 모델에서 인터페론 조절인자와 산화질소 합성요소의 발현

현준영,DebasishSinha,TerrenceP.O’Brien,이진학,위원량,.Joon Young Hyon. M.D.... Debasish Sinha. Ph.D... Terrence P. O`Brien. M.D.. Jin Hak Lee. M.D... Won Ryang Wee. M.D... 대한안과학회 2006 대한안과학회지 Vol.47 No.10

Purpose: To investigate the expression of IRF-1, IRF-7 and iNOS in the mice model of Pseudomonas aeruginosa keratitis. The enzymatic activity of iNOS and its expression were also investigated. Methods: With western blot analysis, the protein expression of IRF-1, IRF-7 (at 24 hours), and iNOS (at 12 hours and 24 hours) were evaluated in the mouse model of P. aeruginosa keratitis. iNOS enzymatic activity was determined with a scintillation counter. IRF-1 and IRF-7 expression were localized with immunofluorescent labeling. The wounded control group was given the same corneal wound without bacterial inoculation, and the fellow eyes served as normal controls. Results: Expression of IRF-1, IRF-7 and iNOS was highly upregulated in corneas with P. aeruginosa keratitis compared to normal or wounded corneas. iNOS enzymatic activity also was higher in infected than normal corneas. In wounded corneas, NOS2 expression and activity slightly increased at 12 hours after the infection. Intense IRF-1 immunopositivity was seen in the epithelial layer of infected corneas. Some corneal stromal cells and endothelial cells showed moderate positive labeling in infected corneas. IRF-7 showed intense labeling in the epithelial layer and endothelial cells of normal as well as infected corneas. Increased IRF-7 labeling was observed in epithelial cells in the ulcerated region of infected corneas. Conclusions: These results suggest that IRF-1, IRF-7 and iNOS may play a regulatory role in the immune responses and wound healing process in P. aeruginosa keratitis.

전산CP 적용의 활성화를 위한 사용자 의견 조사

현준영,소은영,최은영,이정희,정은자 한국의료QA학회 2008 가을학술대회 Vol.2008 No.3

표준진료지침 활성화와 수술 예방적 항생제 사용 적정성 증진

현준영,정숙향,최은화,김홍빈,전귀늠,이정희,문소영,소은영,김형호,정진엽 한국의료QA학회 2009 가을학술대회 Vol.2009 No.2

류마티스질환과 연관된 전안부질환

현준영 대한의사협회 2016 대한의사협회지 Vol.59 No.1

Rheumatic diseases are associated various extra-articular manifestations, such as eye, nerve, pericardium, and pleura. The anterior part of the eye has distinguished anatomic structure resembling synovial joints, thus it is a common site of ocular manifestation in rheumatic disease. These changes include sicca syndrome, peripheral ulcerative keratitis, episcleritis, scleritis, and iritis. Some of these findings may be the clues for the diagnosis of the rheumatic diseases, and some ocular manifestations may represent the activity or prognosis of the rheumatic diseases. These ocular complications may leads to severe visual loss. It is crucial to rule out underlying systemic diagnosis in rheumatic disease associated ocular diseases, and when diagnosed, the coordination of the rheumatologist with the ophthalmologist in the treatment is imperative.

Korean Guidelines for the Diagnosis and Management of Dry Eye: Development and Validation of Clinical Efficacy

현준영,김효명,이도,정의상,송종석,최철영,이정복,the Korean Corneal Disease Study Group 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.3

Purpose: To evaluate the clinical efficacy of newly developed guidelines for the diagnosis and management ofdry eye. Methods: This retrospective, multi-center, non-randomized, observational study included a total of 1,612 patientswith dry eye disease who initially visited the clinics from March 2010 to August 2010. Korean guidelinesfor the diagnosis and management of dry eye were newly developed from concise, expert-consensus recommendations. Severity levels at initial and final visits were determined using the guidelines in patients with 90± 7 days of follow-up visits (n = 526). Groups with different clinical outcomes were compared with respect toclinical parameters, treatment modalities, and guideline compliance. Main outcome measures were ocular andvisual symptoms, ocular surface disease index, global assessment by patient and physician, tear film break-uptime, Schirmer-1 test score, ocular surface staining score at initial and final visits, clinical outcome after threemonths of treatment, and guideline compliance. Results: Severity level was reduced in 47.37% of patients treated as recommended by the guidelines. Youngerage (odd ratio [OR], 0.984; p = 0.044), higher severity level at initial visit, compliance to treatment recommendation(OR, 1.832; p = 0.047), and use of topical cyclosporine (OR, 1.838; p = 0.011) were significantly associatedwith improved clinical outcomes. Conclusions: Korean guidelines for the diagnosis and management of dry eye can be used as a valid and effectivetool for the treatment of dry eye disease.

Effect of Macrophage Migration Inhibitory Factor on Corneal Sensitivity after Laser In Situ Keratomileusis in Rabbit

현준영,Stacey Hose,Celine Gongora,Debasish Sinha,Terrence O’Brien 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.2

Purpose: To investigate the effect of macrophage migration inhibitory factor (MIF) on corneal sensitivity afterlaser in situ keratomileusis (LASIK) surgery. Methods: New Zealand white rabbits were used in this study. A hinged corneal flap (160-μm thick) was createdwith a microkeratome, and -3.0 diopter excimer laser ablation was performed. Expressions of MIF mRNA inthe corneal epithelial cells and surrounding inflammatory cells were analyzed using reverse transcription polymerasechain reaction at 48 hours after LASIK. After LASIK surgery, the rabbits were topically given either 1)a balanced salt solution (BSS), 2) MIF (100 ng/mL) alone, or 3) a combination of nerve growth factor (NGF, 100ug/mL), neurotrophine-3 (NT-3, 100 ng/mL), interleukin-6 (IL-6, 5 ng/mL), and leukemia inhibitory factor (LIF, 5ng/mL) four times a day for three days. Preoperative and postoperative corneal sensitivity at two weeks and at10 weeks were assessed using the Cochet-Bonnet esthesiometer. Results: Expression of MIF mRNA was 2.5-fold upregulated in the corneal epithelium and 1.5-fold upregulatedin the surrounding inflammatory cells as compared with the control eyes. Preoperative baseline corneal sensitivitywas 40.56 ± 2.36 mm. At two weeks after LASIK, corneal sensitivity was 9.17 ± 5.57 mm in the BSStreated group, 21.92 ± 2.44 mm in the MIF treated group, and 22.42 ± 1.59 mm in the neuronal growth factors-treated group (MIF vs. BSS, p < 0.0001; neuronal growth factors vs. BSS, p < 0.0001; MIF vs. neuronalgrowth factors, p = 0.815). At 10 weeks after LASIK, corneal sensitivity was 15.00 ± 9.65, 35.00 ± 5.48, and29.58 ± 4.31 mm respectively (MIF vs. BSS, p = 0.0001; neuronal growth factors vs. BSS, p = 0.002; MIF vs. neuronal growth factors, p = 0.192). Treatment with MIF alone could achieve as much of an effect on recoveryof corneal sensation as treatment with combination of NGF, NT-3, IL-6, and LIF. Conclusions: Topically administered MIF plays a significant role in the early recovery of corneal sensitivity afterLASIK in the experimental animal model.

소아의 눈물기관폐쇄에서 단누소관을 통한 눈물관의 실리콘관삽입술

현준영(Joon Young Hyon),곽상인(Sang In Khwarg),장봉린(Bong Leen Chang),위원량(Won Ryang Wee),이진학(Jin Hak Lee) 대한안과학회 2001 대한안과학회지 Vol.42 No.4

Effect of Donor Age on Graft Survival in Primary Penetrating Keratoplasty with Imported Donor Corneas

권현윤,현준영,전현선 대한안과학회 2020 Korean Journal of Ophthalmology Vol.34 No.1

Purpose: To investigate the influence of donor age on corneal graft survival following primary penetrating keratoplasty(PK) with imported donor corneas. Methods: The eyes of patients who underwent primary PK with imported donor corneas were classified retrospectivelyinto two groups according to a donor-age cutoff of 65 years. Primary outcome measures were rejection-free graft survival and graft survival. Cox proportional hazard regression analysis was used to assessthe factors affecting graft survival. Survival analysis was performed using the Kaplan-Meier method, whiledifferences between groups were examined using a log-rank test. A subgroup analysis of low- and high-riskeyes according to preoperative diagnosis was also performed. Results: A total of 140 eyes from 138 patients (age, 58 ± 18 years) were enrolled. Cox regression analysis revealedthat the donor age of 65 years or older group presented an increased risk of both graft rejection andfailure. Survival analysis revealed that rejection-free graft survival and graft survival rates were higher in eyesin the donor age of less than 65 years group. Finally, in the subgroup analysis, both rejection-free graft survivaland graft survival rates were significantly higher in the donor age of less than 65 years group than in the donorage of 65 years or older group, but only in the low-risk subgroup. Conclusions: Donor age may correlate with graft survival in primary PK performed with imported donor corneas. Donor age could be a considerable factor in primary PK with imported donor corneas, especially in preoperativelylow-risk patients.

Current Practice Pattern for Dry Eye Patients in South Korea: A Multicenter Study

송종석,현준영,이도,정의상,최철영,이정복,김효명 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.2

Purpose: To assess current practice patterns for dry eye patients in South Korea and to evaluate the preferenceaccording to the ages and clinic types of physicians. Methods: Dry eye patients (n = 1,612) were enrolled in this multicenter cross-sectional, observational study. Theseverity level of dry eye patients was classified based on the Korean guidelines for dry eye treatment. Themedical records of the enrolled dry eye patients were evaluated, and the practice styles and the preferenceswere analyzed according to the ages and clinic types of physicians. Results: Of all patients, dry eye level 1 was most common (47.5%), followed by level 2 (33.5%), level 3 (9.1%), andlevel 4 (1.1%). Topical anti-inflammatory agents were used in 70.7% of patients with dry eye level 2 and in 80.6%of patients at levels 3 and 4. Topical anti-inflammatory agents were also used in 48.7% of patients with dry eyelevel 1. Preservative-free artificial tears were preferred at all dry eye levels. The use of topical anti-inflammatoryagents did not differ with investigator ages, but older physicians preferred preserved artificial tears more thanyounger ones. Physicians at referral hospitals also tended to use topical anti-inflammatory agents and preservative-free artificial tears earlier, beginning at dry eye level 1, than those who worked at private eye clinics. Conclusions: Topical anti-inflammatory agents were commonly prescribed for the treatment of dry eye patientsin South Korea, even from dry eye level 1. Preservative-free artificial tears were preferred at all dry eye levels. Practice styles differed somewhat depending on the ages and clinic types of physicians.

Contact Lens Induced Limbal Stem Cell Deficiency: Clinical Features in Korean Patients

이승찬,현준영,전현선 대한안과학회 2019 Korean Journal of Ophthalmology Vol.33 No.6

Purpose: To describe the clinical features of Korean patients with contact lens-induced limbal stem cell deficiency(CL-LSCD). Methods: Medical records of 22 patients who were diagnosed with CL-LSCD between 2014 and 2019 werereviewed retrospectively. Outcome measures included demographics, clinical presentation, treatment, clinicalcourse, and pattern of contact lens (CL) wear. Results: Forty-two eyes of 22 patients were found to have typical changes associated with CL-LSCD. Twenty(91%) patients were women and mean age was 36 ± 12 years. All patients had myopia with mean sphericalequivalent of -7.52 ± 3.2 diopter. Twenty (91%) patients had bilateral disease and the location of limbal involvementwas diffuse in 20 eyes (47.6%) and partial in 22 eyes (52.4%, superior in 20 eyes and inferior in 2 eyes). Fourteen (63.6%) patients complained of decreased visual acuity. Average period of CL wear was 14 ± 9years. Four patients used cosmetic colored CLs and four patients had a history of overnight CL wear. All 12patients who completed follow-up (28 ± 42 weeks) showed improvement in visual acuity and ocular surfacecondition after cessation of CL wear and medical treatment. Of them, five (42%) patients showed full recoverywhile seven (58%) showed partial recovery. Conclusions: If a patient with a history of CL wear for an extended period of time presents with decreased visualacuity, practitioners should perform detailed examinations with suspicion of CL-LSCD, including fluoresceinstaining. CL-LSCD is usually reversible and close follow-up with conservative treatment is recommended asthe initial treatment option.