인체폐암세포 NCI-H460 및 A549의 apoptosis 유발에 미치는 삼기보배탕의 영향

허만규,허태율,김기탁,변미권,김진영,심성흠,김광록,감철우,박동일,Heo, Man-Kyu,Heo, Tae-Yool,Kim, Ki-Tak,Byun, Mi-Kwon,Kim, Jin-Young,Sim, Sung-Heum,Kim, Koang-Lock,Kam, Cheol-Woo,Park, Dong-Il 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.3

Objectives : This study was designed to investigate the antiproliferative activity of the water extract of Samgibopae-tang (SGBPT) in NCI-H460 and A549 non-small-cell lung cancer cell lines Methods : In this study, we measured the subsistence, form of NCI-H460 and A549 non-small-cell lung cancer cell by hemocytometer and DAPI staining. In each cell, we analyzed DNA fragmentation. reverse transcription-polymerase chain reaction and measured activity of caspase-3, caspase-8 and caspase-9. Results and Conclusions : We found that exposure of A549 cells to SGBPT resulted in growth inhibition in a dose-dependent manner. butSGBPT did not affect the growth of NCI-H460 cells. The antiproliferative effect by SGBPT treatment in A549 cells was associated with morphological changes. SGBPT treatment partially induced the expression of DR5 cells and the expression of Faswas markedly increased in both transcriptional and translational levels in A549 cells. SGBPT treatment partially induced the expression of Bcl-2, Bcl-XL and the expression of Bid was markedly decreased in translational levels in A549 cells. However, SGBPT treatment did not affect the expression of IAP family in A549 orNCI-H460 cells. SGBPT treatment partially induced the expression of caspase-3, caspase-8, caspase-9 activity which markedly increased in a dose-dependent manners in A549 cells. The fragmental development of PARP and ${\beta}$-catenin protein was observed in A549 cells by SGBPT treatment. SGBPT treatment induced the expression of PLC-${\gamma}1$ protein which decreased in A549 cells. SGBPT treatment partially induced the expression of DFF45/ICAD which markedly increased in a dose-dependent manner in A549 cells. Taken together. these findings suggested that SGBPT-induced inhibition of human lung carcinoma did not affect NCI-H460 cell growth. However, SGBPT-induced inhibition of human lung carcinoma A549 cell growth was associated with the induction of death receptor and mitochondrial pathway. The results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of SGBPT.

Genetic Diversity and Population Structure of Pseudobagrus fulvidraco in the Nakdong River

허만규,최주수,허윤성,이복규,Huh, Man-Kyu,Choi, Joo-Soo,Heo, Youn-Seong,Lee, Bok-Kyu Korean Society of Life Science 2007 생명과학회지 Vol.17 No.7

전분 젤 전기영동을 사용하여 한국내 분포하는 동자개 여섯 집단에서 유전적 다양성과 집단구조를 평가하였다. 14개 대립유전자좌위에서 9좌위가 다형현상을 나타내었다(64.3%). 종수준과 집단수준에서 유전적 다양성은 각각 0.286과 0.277이였다. Wright의 고정지수에서 Hardy-Weinberg 평형에 비해 전반적인 이형접합체 결핍이 나타났다. 이 결핍은 집단내 유효한 개체수의 부족을 시사한다. 집단간 유전적 분화 정도는 0.064로 대부분의 유전적 변이(93.6%)가 집단내에 있음을 의미한다. 간접적으로 평가된 집단간 이주하는 개체수는 3.67로 나타났다. 제한된 유전자 유동과 유효집단의 감소는 유전적 부동을 유발하고, 주기적인 포획으로 인한 개체수의 감소는 유전자 상실로 이어지고 있다. 대부분의 집단이 겨울철 가뭄과 여름철 홍수로 심한 병목을 겪고 있었다. Enzyme electrophoresis was used to estimate genetic diversity and population genetic structure of Pseudobagrus fulvidraco in Korea. Nine of the 14 loci (64.3%) showed detectable polymorphism. Genetic diversity at the population and species levels were 0.286 and 0.277, respectively. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a substantial deficit of hetero-zygotes relative to Hardy-Weinberg expectations. This deficit is expected that it is due to a limited effective number of individuals per population. The average $G_{ST}$ for polymorphic loci was 0.064, indicating that most (93.6%) of the genetic diversity occurred within populations. The indirect estimate of gene flow based on mean $G_{ST}$ was 3.67. Given limited gene flow is expected to diverge genetically due to drift and reduced populations. Most populations in our study experience annual, severe demo-graphic bottlenecks due to drought and floods.

인체폐암세포 NCI-H460 및 A549의 증식에 미치는 參芪補肺湯의 영향 비교

허만규(Man Kyu Heo),박철(Cheol Park),최영현(Young Hyun Choi),감철우(Cheol Woo Kam),박동일(Dong Il Park) 한의병리학회 2007 동의생리병리학회지 Vol.21 No.4

해양 갑각류 민꽃게(Charybdis japonica) 집단의 형태학적 변이성

허윤성,이복규,허만규,Heo, Youn-Seong,Lee, Bok-Kyu,Huh, Man-Kyu 한국생명과학회 2006 생명과학회지 Vol.16 No.4

In order to determine the morphological variations of the Japanese swimming crab, Charybdis japonica, we conducted morphological analysis of five populations that are relatively abundant in Korea and Japan. Abdominal sternite for individuals of the Tean population showed significantly short when compared to those of other populations. Principal component analysis demonstrated that morphological differentiation was due to differences (A/Q, B/M, D/P, F/O, J/K), and effectively discriminated each others. The first two components accounted for 40.9% and 60.7% of the variation, respectively. The dendrogram showed three distinct groups, Tokyo population, Taean population, and the other populations.

Genetic Diversity and Population Structure of Codium fragile (SURINGAR) HARlOT in Korea Using Allozymes

이복규,박소혜,허윤성,주무열,최주수,허만규,Lee Bok-Kyu,Park So-Hye,Heo Youn-Seong,Ju Mu-Teol,Choi Joo-Soo,Huh Man-Kyu Korean Society of Life Science 2006 생명과학회지 Vol.16 No.2

The study of genetic diversity and population structure was carried out in the Codium fragile using allozyme analysis. Although this species has been regarded as a ecologically and economically important source, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of four Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level ($H_{ES}$=0.144), and, that of the population level was relatively low ($H_{EP}$=0.128). Nearly 87% of the total genetic diversity in C. fragile was apportioned within populations. The predominant asexual reproduction, population fragmentation, low fecundity, geographic isolation and colonization process are proposed as possible factors contributing to low genetic diversity in this species. The indirect estimated of gene flow based on $G_{ST}$ was 1.69. The moderate level of gene flow in C. fragile populations is mainly caused by thallus developed from isolated utricles dispersal via sea current. 알로자임 분석을 이용하여 청각의 유전적 다양성과 집단구조를 분석하였다. 이 종은 한국내 생태적, 경제적 중요한 자원이지만 유전적 분석이 수행되지 않았다. 전분 젤 전기영동으로 이 종의 한국내 네 집단에 대해 알로자임 변이와 유전 구조를 조사하였다. 15개 대립유전자좌위에 대해 9개 좌위(60.0%)가 적어도 한 집단에 대해 다형현상을 나타내었다. 종수준에서 유전적 다양성은 매우 높았다($H_{ES}$=0.144). 집단수준에서 유전적 다양성은 비교적 낮았다($H_{EP}$=0.128). 청각에서 전체 유전적 다양도의 87%는 집단내에 내포되어 있었다. 청각의 번식방법은 유성생식보다는 무성생식이 우세하고, 집단의 단절, 낮은 자손의 생성, 지리적 격리, 그리고 정착과정이 낮은 유전적 다양성을 설명하는 요인으로 사료된다. 조사한 청각 집단에서 세대당 이주하는 개체수는 1.69로 평가되었다. 이 값은 보통 수준의 유전자 흐름으로 해류를 통한 이동이 주된 요인으로 보인다.

八物湯이 알레르기반응에 미치는 효과

허만규,홍현우,감철우,박동일 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4

This experimental research has been done to study the effects of Palmul-tang on the anti-allergic reaction. We found the several important results from the research which has been performed by two experiments toward immediately type and delayed type in order to study the effects of Palmul-tang on hypersensitivity response to mice. The results obtained from our research are as following: The survival rate of one group to which we injected only the compound 48/80 is almost 0% according to its density and timing test. In the other hand, the survival rates of the other group to which we injected both of the compound 48/80 and Palmul-tang are 20%, 0%, 40%, 10%, 20% and 50% according to 0.025, 0.05, 0.1, 0.25 0.5 and 1 (mg/g) of compound 48/80. Time dependency test also shows the 30% and 20% survival rates in 5 and 1 O minutes. Palmul-tang revealed the significantly inhibitory effect on Compound 48/80 induced Mast cell degranulation. Palmul-tang showed the significantly inhibitory effect in the delayed type hypersensitivity response to picry1 chbride. Palmul-tang showed the significantly inhibitory effect in the delayed type hypersensitivity response to sheep red blood cell. Our research provides the important evidence that Palmul-tang is benificial to the prevention and treatment of allergy related oiseases.