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허남기,김경대,최병곤,김경희,민황기,권혜정,Heo Nam-Kee,Kim Keyung-Dea,Choi Byoung-Gon,Kim Keyung-Hee,Min Hwang-Kee,Kwon Heuy-Jeong 한국작물학회 2005 한국작물학회지 Vol.50 No.suppl1
본 시험은 식품의 장기간 저장이 가능하고 간편하게 유통할 수 있는 Retort pouch 기술을 찰옥수수에 접목시켜 저장 중 품질유지 및 저장비용 절감을 위하여 찰옥수수 레토르트 가공 적성을 분석한 결과는 다음과 같다. 1. 식품적성은 식품공전에 의한 저장전 레트로트 식품규격에 따른 성상 및 세균 조사결과 모든 처리에서 팽창되지 않았으며 세균검출 시험결과도 음성으로 나타났다. 2. 미생물 활성과 부패에 관여하는 수분활성도 측정결과 30일 저장후 부터 레토르트 공정전 전처 리로서 열수처리에서는 일반곰팡이 번식 조건인 0.80보다 높은 $0.81\~0.87$로 나타났으며 60일 후에 낮아지는 경향이었고 증숙처리, 급속냉동처리 등에서는 일반 부패세균 및 곰팡이 번식조 건 0.80 이하로 안정성으로 나타났다. 3. 색도색차는 냉동+레토르트 처리시 타처리에 비하여 어두워 외관상 상품성이 떨어졌고 레토르트 처리시 $121^{\circ}C$의 10분간은 색깔이 우수하였고 저장조건은 $5^{\circ}C,\;15^{\circ}C$ 보다 $-20^{\circ}C$ 냉동저장이 다소 우수하였으나 큰 차이는 없었다. 4. 저장기간에 따른 유리당 함량은 저장조건에 따른 미량의 차이는 있었지만 뚜렷한 경향은 없었다. 5. 식미는 저장 60일부터 레토르트 공정전 무처리, 열수처리 및 급속냉동처리에서는 식미가 떨어지고, 이취가 발생하여 저장성이 떨어졌으나 증숙 처리한 옥수수는 $5^{\circ}C,\;15^{\circ}C$ 저장시 $20^{\circ}C$에 냉동 저장된 시료와 큰 차이 없이 양호하였다. Retort pouch is widely used in food industry for a long-term preservation and safe food production. By applying retort pouch technique to waxycorn storage, the quality of waxycorn could be maintained and the storage expense could be saved during storage. Water activities(Aw) of retort waxycorn were below 0.80 except blanching treatment, and it is known that microbial propagation is subdued below 0.80. Commercial value of waxy corn was deteriorated when it was frozen quickly at $-40^{\circ}C$ before treating Retort due to obscurity of chromatocity, while the color change was not noticeable when it was treated with Retort at $121^{\circ}C$ for 10min. For all treatments, very small amounts of free sugar were detected, however, there were no significant differences between treatments. As storage period was longer, shelf lifes of waxy corn in control and waxy corn treated with blanching were more shortened when waxy corn was stored at $15,5^{\circ}C$ before Retort, while waxy corn with boiling treatment was not significantly different compared with that in storage in freezer.
고위도 지역에서 내한성 차나무 육성을 위한 국내외 수집종의 생육 및 특성분석
허남기(Nam-Kee Heo),이광재(Kwang-Jae Lee),김희연(Hee-yeon Kim),임상현(Sang-hyen Leem),함헌주(Heon-Joo Ham),김경희(Kyeng-Hee Kim),이철훈(Cheol-Hun Lee),한상섭(Sang-seob Han) 한국차학회 2010 한국차학회지 Vol.16 No.2
This study investigated the genetic characteristics of tea tree lines collected both in Korea and abroad via a functional analysis of growing features such as cold hardiness. The purpose of this study was to determine the conditions required for the stable cultivation of tea trees in Goseong at high altitudes and under climatic conditions less favorable than those in southern regions. In accordance with the analysis, 8 lines including GS 1 showed stronger cold hardiness than Yabukida, the standard variety, and the harvest period was faster by 1 to 4 days compared to Yabukida. The number of leaves per stock was 102 to 155 in 6 lines. In accordance with the analysis on indoor freezing (EC), 16 lines had high resistance to freezing with LT50 (winter killing temperature) from -10.15 to -12.42oC. The total catechin content was 4.79 to 7.16%, similar to or lower than that of Yabukida. However, GS 8 and GS 15 had higher GCg and ECg values, respectively. The GS 7 and GS 18 lines had lower caffeine content, whereas the tannin content was lower in the G21 line. RAPD classified the varieties into 3 groups based on 75% similarity. It was found that 3 lines, GS 15, GS 19 and GS 21, were genetically very similar. It was estimated that 5 lines, GS 15, 16, 19, 21 and GS 22, which were selected according to strong cold hardiness, can be grown as promising lines at high altitudes.
서영호,허남기,정영상,이해익,민황기,Seo, Young-Ho,Heo, Nam-Kee,Jung, Yeong-Sang,Rhee, Hae-Ik,Min, Hwang-Kee 한국토양비료학회 2000 한국토양비료학회지 Vol.33 No.2
옥수수의 항산화 활성에 대한 N-P-K의 시비의 효과를 알아보고자, 찰옥 2호와 수원 19호 두 품종을 대상으로 시비량에 따른 옥수수의 항산화 활성과 tocopherols, 페놀화합물, carotenoids의 함량을 분석하였다. 시비 수준은 N, P, K 각각에 대한 표준 시비량과 이의 1/2 및 2배 그리고 무비이었다. 찰옥2호의 항산화 활성은 19.0~22.2%로, 수원19호보다 높았다. 찰옥 2호의 항산화 활성은 표준 시비구에서 가장 높았으나, 처리간의 유의성은 없었고, 수원19호의 항산화활성과 항산화 성분 함량은 질소 2배 시용구에서 높았다. Tocopherols, 페놀화합물, carotenoids의 함량은 찰옥2호의 경우 각각 30.0~38.1, 104.7~118.8, $0.1{\mu}gml^{-1}$였고, 수원19호는 각각 16.7~20.1, 59.9~72.7, $35.5{\sim}50.0{\mu}gml^{-1}$로 항산화 활성과 마찬가지로 변이폭이 작고, 시비량에 따른 뚜렷한 경향을 보이지 않았다. ASI와 항산화 활성은 옥수수의 수량과 정상관이 있었으며, 항산화 활성은 tocopherols, 페놀화합물의 함량과 정상관이 있었으나 carotenoids와는 상관관계가 없었다. 옥수수의 항산화 활성과 항산화성분 함량은 품종간 변이의 영향이 시비량에 의한 환경변이의 영향보다 큰 것으로 보인다. Antioxidant activity and related components including tocopherols, phenolic compounds and carotenoids in the corn(Zea mays L.) kernels were measured to understand effects of fertilizer application. Hybrids used were Chalok 2, a waxy corn, and Suwon 19, a dent corn. The standard fertilizer level of N-P-K was 14.5-3-6 for Chalok 2 and 17.4-3-6.9 for Suwon 19. The treatments of the fertilizer levels were the standard level, half and two fold amounts of N, P, and K, and no fertilizer. The antioxidant activity was determined by measuring electron donating ability. The antioxidant activity of Chalok 2 was higher than Suwon 19. The antioxidant activity was the highest at the standard fertilizer treatment for Chalok 2, but was statistically not significant. The antioxidant activity and the related compounds content in Suwon 19, however, were the highest in the two fold nitrogen treatment, and the differences were significant. The contents of tocopherols, phenolic compounds and carotenoids were 30.0~38.1, 104.7~118.8, $0.1{\mu}gml^{-1}$, respectively for Chalok 2 and 16.7~20.1, 59.9~72.7, $35.5{\sim}50.0{\mu}gml^{-1}$, respectively for Suwon 19. The antioxidant activity was positively correlated with the grain yield in both cultivars. The shorter the ASI the higher the yield of the plant. The difference in genetic factors would affect greater influence than fertilizer effect on the antioxidant activity of corn.
이재형 ( Jae Hyoung Yi1 ),허남기 ( Nam Kee Heo ),최병곤 ( Byung Gon Choi ),박은희 ( Eun Hee Park ),권세영 ( Se Young Kwun ),김명동 ( Myoung Dong Kim ),홍운표 ( Wun Pyo Hong ),여수환 ( Soo Hwan Yeo ),백성열 ( Seong Yeol Baek ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2
본 연구에서는 강원전통장류로부터 혈전용해 활성이 우수한 효모를 분리하였다. 된장에서 분리한 AFY-1 균주는 혈전용해 활성 측정결과 양성대조구인 plasmin 보다 약 1.75배 높은 활성을 나타내었다. 분리한 효모는 18S rRNA 염기서열 및 탄소원 이용 특성 분석을 통하여 Saccharomycetales sp.로동정되었으며, AFY-1 균주의 생육 최적온도는 32oC였다. 본 연구에서 분리된 혈전용해능이 우수한 효모균주는 안전성 검증 등 추가연구를 통해 발효식품 제조용 스타터로서 활용이 가능할 것으로 기대된다. Yeast strains demonstrating fibrinolytic activity were isolated from traditional fermented soybean in Gangwon province, Korea. The AFY-1 strain isolated from fermented soybean paste showed the highest fibrinolytic activity (3.5 U/mg protein) corresponding to a 1.75 fold higher fibrinolytic activity compared with the plasmin (2.0 U/mg protein). The optimum temperature for the growth of AFY-1 strain was 32oC. Analysis of 18S rRNA gene sequence and carbon source utilization pattern indicated that the AFY-1 strain shares the highest homology (99%) with Saccharomycetales sp.