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흰쥐에 Vitamin Antioxidants 투여가 Lipid Peroxidation에 미치는 영향
함윤애,홍영숙,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1982 EMJ (Ewha medical journal) Vol.5 No.3
We observed the effect of the administration of vitamin A,C and E with β-naphthoflavone and piperonyl butoxide on cytochrome P-450 level and lipid peroxidation in rats liver. The level of hepatic cytochrome P-450 decreased after vitamin A,C and E was administration. In contrast, β-naphthoflavone, when administered with vitamin A,C and E, the increase of cytochrome P-450 was prevented. Lipid peroxidation was decreased after vitamin A, C, and E was administer-ed. Moreover when β-naphthoflavone was administered together with vitamin A, C and E lipid peroxidation was not increased. When piperonyl butoxide was administered together with vitamin A,C and E, both cytochrome P-450 and lipid peroxidation were decreased. These results indicate that vitamin antioxidants can prevent lipid peroxidation by a cytochrome P-450 dependent terminal oxidase system in rat liver microsomes.
착상전 임신기간 중 흰쥐에 있어서 Cytochrome P-450의 변화
함윤애,김성례,홍영숙,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1980 EMJ (Ewha medical journal) Vol.3 No.2
The levels of hepatic microsomal cytochrome P-450 in preimplantation rats were determined 1) The activities of the hepatic microsomal cytochrome P-450 associated with the events leading to implantation were gradually increased in preimplantation rats. 2) The level of the hepatic microsomal cytochrome P-450 was peak, 2.65 nmoles/mg protein, on day 4 in normal preimplantation rat. 3) The activity of the hepatic microsomal cytochrome P-450 was decreased in ovariectomized rat than normal rat. 4) The hepatic microsomal cytochrome P-450 level in ovariectomized rat was peak, 2.39 nmoles/mg protein, on day 5. 5) We observed the fact thar the activity of microsomal P-450 was delayed in ovariectomized rat. 6) We confirmed the fact that the hepatic microsomal cytochrome P-450 level was affected by steroid hormone.
생쥐에 있어서 성주기와 착상전 임신기간 중 Cytochrome P-450의 변화
함윤애,김성례,홍영숙,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1979 EMJ (Ewha medical journal) Vol.2 No.4
Cytochrome P-450 is sometimes called the microsomal carbon monoxide-binding pigment. It is present in the microsomal fraction of several animal tissues, and also in the mitochondrial fraction of adrenal cortex, but not in the mitochondria of other tissues. Cytochrome P-450, the liver microsomal drugmetabolizing enzyme is membranebound and fraction as multicomponent electron transport system for the metabolism of a variety of endogenous substrates(such as steroids, fatty acids, and bile acids) and exogenous substance (such as drugs, carcinogens, insecticides, and many other foreign compounds). The effect estrous cycle and preimplantation on the levels of hepatic microsomal cytochrome P-450 in mice were determined. In effect of estrous cycle on microsomal cytochrome P-450 level, the most increment was at estrus. The content of cytochrome P-450 was increased significantly at pregnant Day 3 of preimplantation.
스테로이드 홀몬에 의한 UDP-Glucuronosyltransferase의 발현과 기질 특이성에 관한 연구
홍영숙,함윤애,이세윤 梨花女子大學校 韓國生活科學硏究院 1992 韓國生活科學硏究院 論叢 Vol.50 No.-
The cDNA encoding a member of a family of steroid-UDP-glucuronosyltransferases has been cloned and sequenced. The form of UDP-glucuronosyltransferase designed UDPGT_r-5 is one of a number of closely related species encoded by member of multigene family. In this work northern analysis of a new member of this steroid glucuronidating family of UDP-glucuronosyltransferases is revealed by expression of synthetic oligonucleotide of UDPGT_r-5, 45 specific oligomer and 18 common oligomer nulceotides. Liver RNAs were separated from AD, PB, retinyl acetate, and insulin treated rats. UDPGT_r-5 oligomer hybridization and RNAs were detected by autoradiography. In UDPGT_r-5-specific oligomer hybridization, the 2.3Kb species were active towards androstenedione and retinyl acetate, while UDPGT_r-5 common oligomer active toward retinyl acetate and phenobarbital. Insulin induced RNA was no change 2.3Kb and 3.5Kb in UDPGT_r-5. This result suggested that the expression of UDPGT_r by insulin is controlled at the post translational level. These data showed that UDPGT_r-5 are androstenedione and retinyl acetate UDP-glucuronosyltranferse that is encoded by two mRNAs transcripted from a single gene.
김정자,함윤애,강지용,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1978 EMJ (Ewha medical journal) Vol.1 No.1
Total cholesterol content of fishes and shellfishes, which bought in the local market, was estimated in part (head, muscle, skin, egg, and roe). and also, the general analytical data(water, protein, fat, and ash) were examined in the parts. The results of cholesterol content are as follows; 1) In the fresh water fishes, the cholesterol content was highest in trout and watereel. 2) In the marine fishes, it was high in makerel, Spanish makerel, horse makerel, and hickory sard and low in sea-cucumber and thormbock stingray. 3) Cholesterol content of the parts in both fresh water fishes and marine fishes was highest in roe, and the next egg, skin, head, and muscle in the ordet named. 4) Cholesterol content of the shell fishes was 100mg∼200mg/100g. 5) Generally, the cholesterol content also was high in the sample that fat content was high.
白鼠에 있어서 Hepatic Microsomal Cytochrome P-450에 대한 Diazepam, Cimetidine 및 Sulpiride의 영향
김복희,함윤애,홍영숙,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1979 EMJ (Ewha medical journal) Vol.2 No.3
Cytochrome P-450 is a terminal oxidase of the hepatic microsomal monooxygenase system associated with the oxidative biotransformation of a varity of lipophilic endogenous and exogenous compounds, and generally is assayed by CO-binding spectro-photometry of dithionite-reduced samples. In well fed normal male rats with or without stress condition, the dffect of antiulcer drug; cimetidine, sulpiride and diazepam on the level of microsomal cytochrome P-450 were determined. Only stress condition produced on significant effect in cytochrome P-450 contents. Administration of cimetidine, sulpiride and diazepam in this condition caused a 44.1%~87.5% increment in cytochrome P-450, diazepam produced the most increase. After diazepam treatment of rats, the peak position of cytochrome P-450 shifted to a longer wave length of 452 nm.
알코올을 투여한 흰쥐에서 Vitamin E 투여가 Lipid Peroxidation에 미치는 영향
홍영숙,함윤애,성낙응 梨花女子大學校 醫科大學 醫科學硏究所 1984 EMJ (Ewha medical journal) Vol.7 No.1
Ethanol enhances the activity of microsomal enzyme system and lipid peroxidation. We observed the effect of chronic ethanol administration on cytochrome P-450 level and lipid peroxidation in rat liver microsome. The results were as follows : 1) When rats were administered with ethanol, the level of microsomal cytochrome P-450 was decreased and lipid peroxidation did not change significantly. 2) When vitamin A and E were added incubation medium in each group, lipid per-oxidation was decreased significantly.
태생 및 수유기에 알코올에 노출된 신생 흰쥐의 Liver Microsomal Mixed Function Oxidase System과 Lipid Peroxidation의 변화
홍영숙,함윤애,이희래 梨花女子大學校 醫科大學 醫科學硏究所 1989 EMJ (Ewha medical journal) Vol.12 No.3
Maternal ingestion of alcohol produce not only change of drug metabolism but also proliferation of hepatic smooth endoplasmic reticulum and many developmental defects of the central nervous system. The present investigation examined the effects of maternal consumption of alcohol during pregnancy and/or lactation the activities of electron transfer components, mixed function monooxygenase, UDP-glucuronosyltransferase and lipid peroxidation of neonatal rat liver microsomes. Normal group consisted of neonatal rats whose mothers received standard chow and water. The subject of experimental group were neonatal rats whose mothers were exposed to alcohol during pregnancy and lactation(3 weeks). The results were obtained as follows : The activities of the electron transfer system, such as cytochrome P-450, NADPH-cytochrome C reductase were increased in hepatic microsomes of experimental group. The activities of the mixed function monooxygenase, p-nitroanisole-O-demethylase and the conjugated enzyme, UDP-glucuronosyltransferase were increased in hepatic microsomal experimental group. There was no significant differences between the formation of lipid peroxide of normal and experimental group. These results suggest that prenatal exposure to alcohol are influenced by disturb of liver microsomal drug metabolism, especially during the fetal period.
스테로이드 홀몬에 의한 UDP-Glucuronosyltransferase의 발현과 기질 특이성에 관한 연구
홍영숙,함윤애,이세윤 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-
The cDNA encoding a member of a family of steroid-UDP-glucuronosyltransferases has been cloned and sequenced. The form of UDP-glucuronosyltransferase designed UDPGT_r-5 is one of a number of closely related species encoded by member of multigene family. In this work northern analysis of a new member of this steroid glucuronidation family of UDP-glucuronosyltransferases is revealed by expression of synthetic oligonucleotide of UDPGT_r-5 oligomer hybridization , the 2.3Kb species were active towards androstenedione and retinyl acetate, while UDPGT_r-5 common oligomer active toward retinyl acetate and phenobarbital. Insulin induced RNA was no change 2.3Kb and 3.5Kb in UDPGT_r-5.This resu-lt suggested that the expression of UDPGT, by insulin is controlled at the post translational level. These data showed that UDPGT_r-5 are and rostenedione and retinyl actate UDP-glucuronosyltranferse that is encoded by two mRNAs transcripted from a single gene.