Compensated Gross Registered Tonnage의 槪要

이희일(李熙壹),박용철(朴容喆) 대한조선학회 1981 大韓造船學會誌 Vol.18 No.2

흰쥐 해마의 신경연접에서 Serotonin 2 수용체 소재에 대한 전자현미경 자기방사법적연구

이희래 대한해부학회 1993 Anatomy & Cell Biology Vol.26 No.4

성장기 흰쥐 시각피질의 신경연접에 대한 도은법 및 전자현미경적 연구

이희래,Lee, Hee-Lai 한국현미경학회 1997 Applied microscopy Vol.27 No.4

These studies were performed to observe the morphological changes of synapses in the visual cortex of rat during early postnatal development. Specimens of the visual cortex were taken from rats (Sprague Dawley) at 1, 3, 7, 14 and 21 days of age, and prepared for silver impregnation and electron microscopy. The number of synapse and the length of postsynaptic thickening were increased progressively with age, especially 14 and 21 days. The number of dendritic spine was increased conspicuously on postnatal days 14-21. And asymmetic, curved and axo-spinous synapses were increased markedly at the same ages. The present findings suggest that spurt of synaptogenesis in the rat visual cortex occurs during early postnatal development, especially in second to 3rd week period and asymmetric and/or curved axo-spinous synapse is a matured form of synapse with advanced age.

기니픽 뇌 미상핵의 신경세포 및 신경연접에 대한 전자현미경적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1986 EMJ (Ewha medical journal) Vol.9 No.1

The caudate nucleus of the guinea pig was examined at the ultrastructural level. At least four types of neurons and seven types of synapses are distinguished. The large polygonal neurons are measured 18-25㎛ in diameter and have large amount of cytoplasm with many stacks of granular endoplasmic reticulum, concentrations of ribosomes, and rich Golgi cisternae. The medium-sized, ovoid neurons are the most frequent cells. These neurons are measured 10-15㎛ in diameter and contain a lot of organelles. The medium-sized, polygonal neurons are measured 11-18㎛ in diameter. These cells have cytoplasm with moderate amount of granular endoplasmic reticulum and Golgi cisternae. The small neurons are the least common never cells in the caudate nucleus. They have a nucleus with indentations, little Golgi apparatus and few cisternae of granular endoplasmic reticulum. The axon terminals containing small round vesicles are the most frequent type of synapses. The majority of them make asymmetric contact on dendritic spines of neurons. Some of remainders make symmetric contact with dendrites and somata and others asymmetric contact with the same structures. Few of terminals 'en passant' with small round vesicles contact on dendrites and somata. The terminals containing elongated vesicles contact symmetrically on the dendrits and somata. All of the terminals containing pleomorphic or large round vesicles make synapses in the same manner of elongated vesicle terminal.

우리가 떠나온 집, 시골집

이희인(李羲仁) 새얼문화재단 2011 황해문화 Vol.72 No.-

Harmaline이 마우스 Paneth세포에 미치는 영향에 대한 광학 및 전자현미경적 연구

이희래,최월봉,권흥식 대한해부학회 1976 Anatomy & Cell Biology Vol.9 No.2

출생후 성장과정의 흰쥐 새줄무늬체에서 신경연접의 구조적 변동

이희래,Lee, Hee-Lai 한국현미경학회 1995 Applied microscopy Vol.25 No.4

This study was performed to analyze the morphological changes of synapses during early postnatal periods. Neonatal rats were grouped by 3, 7, 14, 21, 28 and 42day old, and observed the ultrastructural pattern of the synapses in the neostriatum by transmission electron microscope. 1. The number of synapse, the length of postsynaptic thickening and the amount of synaptic vesicles markedly increase during postnatal development 2. The proportion of asymmetric and curved synapses gradually increase by developmental periods. From the above results, it is suggested that the size of synapse increase during post-natal period, and asymmetric synapse are formed from the symmetric type and curved synapse are formed from the plane type.

흰쥐 편도체의 Dopamine 수용체에 대한 전자현미경 자기방사법적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1989 EMJ (Ewha medical journal) Vol.12 No.1

The present study was performed to identify the dopaminergic receptor sites in rat amygdala. Identification of the receptor sites at ultrastructrual level has been done by autoradiography after cerebral ventricular perfusion of ^3H-haloperidol. From 434 examples of silver grains, 5% were labeled in soma, 36% in thick dendrites, 26% in the thin branches of axons and dendrites, 21% in thick axons and terminals and 12% in synapses with synaptic vesicles. In the cases of labeled synapses, all of axon terminals contained small round vesicles and showed asymmetric contact with dendrites. By these results it may be implied that dopamine receptors in the amygdala are located in both pre-and post-synaptic sites of the asymmetric axo-dendritic synapses with small round vesicles and the axons of labeled synapses originated from the dopaminergic neurons in the midbrain.

원숭이 뇌 청반 신경연접의 전자현미경적 관찰

이희래,김순회 梨花女子大學校 醫科大學 醫科學硏究所 1982 EMJ (Ewha medical journal) Vol.5 No.3

This study provides a description of the normal morphology of the synapses of the locus coeruleus in the squirrel monkey, Saimiri sciureus, at the electron microscopic level. The animals were perfused with 2% glutaraldehyde-1% paraformaldehyde fixative in phosphate buffer at pH 7.4. Tissues from the locus coeruleus were postfixed by 2% osmium tetroxide in phosphate buffer at pH 7.4, and dehydrated in graded ethanol and aceton, and embedded in Epon 812. The polymerization was carried out in oven at 60℃. The ultrathin sections were cut on a LKB ultratome, contrasted with uranyl acetate and lead citrate, and examined in a Siemens Elmiskop 1A electron microscope. Special attention during the observation was given to the different types of synapses. Five types of synapses with many characteristics, could be differentiated : small-round vesicle terminals(52%), pleomorphic vesicle terminals(25%), elongated vesicle terminals(16%), small-round and dense core vesicle terminals(5%) and large-round vesicle terminals(2%). Some of the axosomatic synapses with small round or pleomorphic vesicles make an indirect contact to the soma by somatic appendages.

흰쥐 선조체 및 흑질의 Serotonin 수용체에 대한 ^3H-ketanserin을 이용한 전자현미경 자기방사법적 연구

이희래,이혜성,배영숙 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-

흰 쥐의 측뇌실에 ^3H-ketanserin을 주입한 다음 신줄무늬체 및 흑색질에서 serotonin 수용체의 분포양상을 전자현미경 자기방사법으로 관찰하여 다음과 같은 결과를 얻었다. 신줄무늬체에서 은입자는 대부분이 가지돌기 및 축삭가지와 연접에 표지되었다. 표지된 연접에서 축삭종말은 소원형 및 다형소포를 함유하였고 비대칭의 축삭가지돌기연접을 형성하였다. 흑색질에서 표지양상은 신줄무늬체와 거의 같았다. 이러한 결과에 따라서 신줄무늬체 및 흑색질에서 serotonin-2 수용체는 주로 소원형 및 다형소포를 함유한 비대칭의 축삭가지돌기연접에 분포하며 이 수용체의 분포는 serotonin신경섬유의 분포와 밀접한 관련이 있는 것으로 생각된다. It has been demonstrated that some serotoninergic axonal branches from the dorsal rap heneurons prject to the neostriatum and the substantia nigra, and also has been reported that serotonin receptors distribute in the same regions of the mammalian brain. It is interesting to analyse how the distribution of serotonin receptors correlates with that of serotoninergic nerve terminals in the brain. This study was performed to identify the serotoninergic nerve terminals in the neostriatum and the substantis nigra of the rat. Identification of the serotoninergic nerve terminals at ultrastructural level has been done by autoradiography after cerebroventricular administration of ³H-ketanserin as a ligand. In the neruropil of the neostriatum, most of silver grains were labeled on the thin branches of axons and dendrites and some of them on soma or myelinated axons. In the cases of labeled synapes, axon terminals contained round and pleomorphic vesicles and showed asymmetric contact with dendrites or dendritic spines. In the substantia nigra, the distribution patterns of labeled siver grains beared a close paralell to neostriatum. But in the labeled synapes from this region, axon terminals ocntaining small round vesicles made mainly asymmetric synapses with dendrites. The observations made in this study indicate that serotonin-2 receptors are present in the asymmetric axo-dendritic synapses with small round and pleomorphic vesicles and the distribution of the serotonin-2 receptors have closely with that of serotoninergic fibers in the neostriatum and the substantia nigra of rat.