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김동현 ( Dong Hyun Kim ),박경희 ( Kyung Hee Park ),김영주 ( Young Joo Kim ),김준호 ( Jun Ho Kim ),한희재 ( Hee Jae Han ),박혜정 ( Hye Jung Park ),박중원 ( Jung Won Park ),이재현 ( Jae Hyun Lee ) 대한천식알레르기학회 2015 Allergy Asthma & Respiratory Disease Vol.3 No.3
Food-dependent exercise-induced anaphylaxis (FDEIA) is a specific variant of anaphylaxis that requires both vigorous physical activity and the ingestion of specific causative foods. In particular, occurrence for FDEIA is rarely associated with apples. A 17-year-old male experienced generalized urticaria, dyspnea, headache, vomiting, and presyncope after ingestion of an apple and then 2 hours of exercise. The skin prick test showed a strong positive reaction to apple crude allergen extract, whereas the results of an open food challenge and exercise provocation tests were negative. However, the exercise test after apple consumption provoked a positive reaction with generalized urticaria, dyspnea, and presyncope. We detected 17 kD IgE-reactive protein band in immunoblotting assay with apple crude extract and patient’s serum.(Allergy Asthma Respir Dis 2015;3:224-227)
검정곰팡이의 분화에 있어서 고인산뉴클레오티드의 출현 및 8-아자구아닌 , 시클로헥시미드의 영향에 관한 연구
김종협,한희재 한국균학회 1984 韓國菌學會誌 Vol.12 No.4
Aspergillus niger van Tieghem was cultured by the method of synchronous and submerged culture. Throughout the culture, sporulation was occured. Highly phosphoryatee nucleotides in sporulating mycelia were detected to assure whether the eucaryotic Aspergillus niger produce these substances or not during the differentiation. Phosphorylated nucleotides were extracted from the conidiophore, bearing mycelia and spore forming body, these nucleotides were identified by TLC with P,E.I. cellulose. Guanosine tetraphosphate was found in both phialide forming mycelia and spore forming body. The contents of free amino acids were assayed and its level was found to increase at early stage of sporulation. The effects of 8-azaguanine examined, it was found to prevent spore formation and to made abnormal structure. The effects of inosinic monophosphate and guanosine monophosphate on spore formation were examined, spore formation was enhanced by these nucleotides.
푸른 곰팡이의 형태분화에 따른 생화학적 변화에 대한 연구
맹윤경,원미선,한희재,김유진,이정자 梨花女子大學校 藥學硏究所 1980 梨花藥學硏究 Vol.- No.20
Differentiation of Penicillium chrysogenum YUFE 1308 during shake and submerged culture was studied. Practical media of penicillium production and synchronizing culture technique were involved in the experiments. Changes of the levels in mycelial ribonucleic acid, total proteins, total carbohydrates, residual sugars, nitrate ions, and pH values are determined. Sporulation of this fungus occurred in submerged culture at 24 hours after replacement when the levels of residual glucose had decreased and nitrogen source had been limited. Optimal range of pH was between 5.4 to 5.8. Synthetic rates of total proteins, total RNAs and total carbohydrates in the mycelia decreased when sporulation is induced. Branching of the hyphae occurred when levels of residual sugars, nitrate ions and pH values had fallen respectively in shake culture at 48 hours after hyphal growth. Antibiotic activities were detectable in the extraction of culture broth from presporulation and sporulating stages.