게 껍질 폐기물로부터 추출한 Chitosan에 Itaconic acid의 그라프트 공중합과 오염물질의 제거 능력

한상문,윤충수,김용범,Han, Sang-Mun,Yun, Chong-Soo,Kim, Yong-Beom 유기성자원학회 1999 유기물자원화 Vol.7 No.2

수산물 가공 공장에서 배출되는 게 껍질 폐기물로부터 Chitin을 추출하고 탈아세틸화하여 Chitosan(Cs)을 제조하였다. 이 Cs를 초산 용액중에서 용해한 후 Itaconic acid를 첨가하고 Ceric ammonium nitrate(CAN)를 반응 개시제로 사용하여 Graft-copolymerization 하였다. 본 연구에서는 Cs에 첨가하는 Itaconic acid와 CAN의 농도, 반응온도, 반응시간의 영향에 따른 최적 중합 조건을 도출하고 이 Grafted-copolymer-의 폐수중의 CODcr 유발물과 구리 이온의 제거 능력을 연구하였다. 여기서 CODcr과 구리이온의 제거능력시험에 사용한 폐수는 도광 공장 폐수를 사용하였고, Graft-copolymerization으로 합성된 이 중합체의 동정에는 적외선 스펙트로메타를 사용하였다. 이상의 실험 결과 Graft율은 Cs에 Monomer로써 Itaconic acid를 0.25M농도로 첨가하였을 때, CAN은 $3.5{\times}10^3M$, 반응 온도는 $40^{\circ}C$ 그리고 반응시간은 4시간일때 가장 높았다. 또한, Cs과 Itaconic acid grafted chitosan(CsIa), Cationic polymer를 사용하여 폐수중 유기물과 금속이온의 제거 실험을 한 결과 상등액의 CODcr과 구리 이온 제거율은 Cs와 Cationic polymer 보다CsIa가 보다 효과적이었다. 이것은 합성한 CsIa가 양쪽성 고분자이기 때문이다. Chitosan was prepared from chitin which was abstracted from wasted crab shells. Then itaconic acid was graft-copolymerized onto chitosan using ceric ammonium nitrate as a reaction initiator. To investigate the optimal grafting conditions, the influences of several factors on the grafting were studied, i. e., the concentrations of CAN and itaconic acid, the reaction temperature and time. And to find out its flocculation ability. the flocculation test was carried out with a metal plating factory waste water. The state of graft-copolymer was identified through IR spectra analysis. The optimal grafting conditions and flocculation results were shown to be : concentration of ceric ammonium nitrate is $3.5{\times}10^3M$, reaction temperature is $40^{\circ}C$ and reaction time is 4hrs with 0.25M of the monomer(itaconic acid). Though flocculation tests using chitosan, grafted chitosan and cation, CODcr. metal ions removal rates were measured. The order of superiority is Itaconic acid grafted chitosan>Chitosan>Cationic polymer.

황산화 키토산 유도체와 수용성 저분자 키토산의 간단한 제조 및 pKas

한상문(Sang Mun Han),김용범(Yong Beom Kim) 한국키틴키토산학회 1999 한국키틴키토산학회지 Vol.4 No.3

N/A To investigate acidity with pKa of water soluble chitosans, low molecular weight chitosans were prepared in mild condition by NaNO2 hydrolysis. The pKas of the water soluble chitosans (MW 3,600, pKa : 6.8, and MW : 10,000, pKa : 6.5) were slightly increased dependent on molecular weight because of decreased acidity of amino group on the chitosans compared with water insoluble chitosan (MW : 50,000, pKa : 6.2). It was suggested that chitosan of low molecular weight having lower reactivity against counter ions and biological substances than water insoluble chitosan. Furthermore, various sulfated chitosan derivatives (N-3,6-O-S-chitosan , N-desulfated 3,6-O-S-chitosan ,3,6-O-S- chitin , and 3,6-O- sulfated -N- ( o-carboxybenzoyl ) chitosan )were synthesized with sulfurtrioxide-pyridene complex in pyridine solvent. Characterization of the sulfated chitosan derivatives was carried out by 13C NMR and IR spectroscopies. To observe ionic reaction properties, pKas of the sulfated chitosan derivatives and chitosan of low molecular weight were estimated by potentiometric titration. The sulfated chitosan derivatives had high water solubility, pKas (pKa : 7.7) of N-3,6-O-S-chitosan and N-desulfated 3,6-O-S-chitosan were increased than pKa of water insoluble chitosan (pKa: 6.2). These results suggest the participation of electrostatic interaction of amino and sulfate groups on the sulfated chitosans.

Synthesis of Sulfated Chitiosan Derivatives and Its Biological Activites

한상문(Sang Mun Han),김용범(Yong Beom Kim) 한국키틴키토산학회 1999 한국키틴키토산학회지 Vol.4 No.1

키토산 분자네트 워크속에서 비타민 C의 지속적 방출 특성

한상문(Sang-Mun Han) 대한환경위생공학회 2005 대한환경위생공학회지 Vol.20 No.2

Chitosan is a dietary fiber because of a linear polysaccharide composed of β-(1→4)-linked 2-amino-2-deoxy-D-glucopyranose. In this study, control release system of vitamin C has been estimated in chitosan molecular network as a vitamin C carrier of controlled release. The amount of released vitamin C were decreased in higher amount of chitosan concentration. Especially, vitamin C were slowly released from chitosan solution in dialysis membrane when compared with vitamin C solution alone in dialysis membrane. These result assumed that chitosan driving force is dependent on chitosan molecular weight and cationic property of amino group with anionic property of vitamin C.

키토산 유도체를 이용한 양쪽성 응집제 및 강력한 금속 흡착제의 개발

한상문(Sang Mun Han),김용범(Yong Beom Kim) 한국키틴키토산학회 1999 한국키틴키토산학회지 Vol.4 No.2

여러 종류의 식용성 유기산을 사용하여 키토산 고분자를 가수분해하는 반응이 연구되었다. 대부분의 유기산은, 특히 반응이 가압상태에서 진행될 때, 매우 효과적이었다. 본 연구에서 고려된 주요 변수는 산의 종류, 농도 및 반응시간과 온도이었다. 젖산이나 아스코브산은 가수분해과정에 있어서 키토산분자의 탈아세틸 정도를 향상시키는 부수적인 효과를 보여주었다. 반응의 한 예로서, 타탈산을 사용하여 가압용기에서 120도(섭씨)1시 간의 반응으로 키토산 고분자 2%를 함유하는 용액의 점소는 반응후 순수한 물과 유사한 정도로 감소되었다. Several human-edible organic acids were used to hydrolyze polymeric chitosan. They were highly effective for the hydrolysis especially when reactions were performed under pressure. Experimental variables were acid moiety, concentration, reaction time and temperature. Some organic acids such as lactic and ascorbic acid promoted the degree of deacetylation in chitosan molecules during the hydrolysis reaction. It was demonstrated that the viscosity of a chitosan solution (at 2 wt%) can be reduced to as low as that of water by a typical autoclave reaction at 120t using tartaric acid for an hour.

황산화 키토산 유도체의 합성과 항에이즈활성

한상문(Sang-Mun Han) 대한환경위생공학회 2006 대한환경위생공학회지 Vol.21 No.1

To investigate anti-HIV-1 activity of water soluble chitosans, sulfated chitosan derivatives were prepared in mild condition. Various sulfated chitosan derivatives (N-3,6-O-S-chitosan, N-desulfated 3,6-O-S-chitosan, 3,6-O-S-chitin, and 3,6-O-sulfated-N-(o-carboxybenzoyl) chitosan) were synthesized with sulfurtrioxidepyridene complex in pyridine solvent. Characterization of the sulfated chitosan derivatives was carried out by ¹³C NMR and IR spectroscopies. To observe ionic reaction properties, pKas of the sulfated chitosan derivatives and chitosan of low molecular weight were estimated by potentiometric titration. The sulfated chitosan derivatives had high water solubility, pKas (pKa : 7.7) of N-3,6-O-S-chitosan and N-desulfated 3,6-O-S-chitosan were increased than pKa of water insoluble chitosan (pKa : 6.2). These results suggest the participation of electrostatic interaction of amino and sulfate groups on the sulfated chitosans. Anti-HIV-1 drugs, such as AZT, ddC, and ddI for anti-HIV activity had higher selective index compared with SCB-chitosan but N-3,6-O-S-chitosan has shown higher selective index compared with ddC and ddI as HIV drugs.. These results suggest that sulfated chitosan derivatives were expected as an anti-HIV drug with differential driving force mechanism against some nucleoside analogs drug in the future.

게 껍질 폐기물로부터 추훌한 Chitosan에 Itaconic acid의 그라프트 공중합과 오염물질의 제거 능력

한상문(Sang-Mun Han),윤충수(Choong-Soo Yun),김용범(Yong-Beom Kim) 유기성자원학회 1999 유기물자원화 Vol.7 No.2

수산물 가공 공장에서 배출되논 게 껍질 폐기물로부터 Chitin을 추출하고 탈아세틸화하여 Chitosan(Cs)을 제조하였다. 이 Cs를 초산 용액중에서 용해한 후 Itaconic acid를 첨가하고 CeIic ammonium nitrate(CAN)를 반응 개시제로 사용하여 Graft-copolymelization 하였다 본 연구에서는 Cs에 첨 7념F는 Itaconic acid와 CAN의 농도, 반응온도, 반응시간의 영향에 따른 최적 중합조건을 도출하고 이 Grafted-copolymel의 폐수중의 CODcr 유발물과 구리 이온의 제거 능력을 연구하였다. 여기서 CODcr과 구리이온의 제거능력 시험에 사용힌 폐수는 도금 공장 폐수를 사용하였고. Graft-copolymerization으로 합성된 이 중합체의 동정에는 적외선 스펙트로메타플 사용하였다. 이상의 실험 결과. Graft 율은 Cs에 Monomer로써 Itaconic acid 릅 0.25M농도로 첨가하였윤 때. CAN은 3.5x 10-\1. 반응 온도는 40.C 그리고 반응시간은 4시간일때 가장 높았다. 또한. Cs과 Itaconic acid grafted chitosan(Csla). Cationic polymer를 사용하여 폐수중 유기불과 금속이온의 제거 실험을 한 결과 상등액의 CODcr과 구리 이온 제거율은 Cs와 Cationic polymer 보다 CsIa가 보다 효과적이었다. 이것은 합성한 Csla가 양쪽성 고분자이기 때문이다. Chîtosan was prepared from chitin which was abstracted from wasted crab shells. Then itaconic acid was graft-copolymerized onto chitosan using ceric ammonium nitrate as a reaction initiator. To investigate the optimal grafting conditions. the influences of several factors on the grafting were studied. i.e .. the concentrations of CAN and itaconic acid. the reaction temperature and time. And to find out its flocculation ability. the flocculation test was carried out with a metal plating factory waste water. The state of graft-copolymer was identified through IR spectra analysis. The optimal grafting conditions and flocculation results were shown to be concentration of ceric ammonium nitrate is 3.5x lQ-1vi. reaction temperature is 40'c and reaction time is 4hrs with O.25M of the monomer(itaconic acid). Though flocculation tests using chitosan. grafted chitosan and cation. CODcr. metal ions removal rates were measured. The order of superiority is Itaconic acid grafted chitosan) Chitosan)Cationic polymer.

Tetracycline을 포접한 Chitin 차페막의 약물 방출특성

한상문 ( Sang Mun Han ),손상현 ( Sang Hyun Son ),조지연 ( Ji Youn Cho ),최한영 ( Han Young Choi ),강병원 ( Byoung Won Kang ),심형진 ( Hyung Jin Shim ),곽병국 ( Byung Kook Kwak ),이승진 ( Seung Jin Lee ) 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.1

Chitin은 생체친화성이 높고, 독성을 유발하지 않는다. 본 연구에서는 Chitin을 calcium chloride dihydrate saturated methanol 용액에 녹여 Chitin 차폐막에 양이온성 항생제인 TC을 포접시켜 TC의 용리 특성을 조사하였다. Chitin 차폐막에 포접시켜 칼슘을 제거한 결과 93% 이상 차폐막속에 포접됨을 알았으며 TC 감수성균의 대부분에 대하여 MIC 농도를 유지하면 방출됨을 확인하였다. Chitin is well known that it is biocompatible, biodegradable and nontoxicity. In this study, chitin was dissolved in a novel solvent newly developed, calcium chloride dihydrate saturated methanol solution. The chitin dope casted into a membrane which was subject to adsorption and release tests with tetracycline. Tetracycline which is cationic antibiotic substance was found to be adhered more than 90% to chitin membrane and was released at the level of minimum inhibitory concentration(MIC) against the tetracycline killing microorgrans. It was concluded that controlled release and adherence of any antibiotic substances from or on chitin membrane depend upon their ionic characters(Carboxy1 group of soluble polysaccharide) and salt concentration of eluents.

심포지움 : 토끼 신동맥에 주입된 키토산 색전물질의 시간 경과에 따른 병리적 변화

한상문(Sang Mun Han),김용범(Yong Beom Kim),곽병국(Byung Kook Kwak),심형진(Hyung Jin Shim),안병제,이용철(Yong Chul Lee),도쿠라세이치(Tokura Seiichi) 한국키틴키토산학회 2002 한국키틴키토산학회지 Vol.7 No.1

키틴과 키토산 유도체의 의료용 고분자재료로써의 응용

한상문 ( Sang Mun Han ),박제권 ( Jae Kweon Park ),안병제 ( Byung Jae Ahn ),이수한 ( Su Han Lee ) 한국키틴키토산학회 2008 한국키틴키토산학회지 Vol.13 No.4