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유영법(Young-Beob Yu),Mi-Jung Kim(김미정),Do-Young Yum(염도영),Bon-Taq Koo(구본탁),Hye-Kyeong Ha(하혜경),Hyun-Kyu Shin(신현규),Jin-Yeul Ma(마진열),Jong-Cheol Park 한국한의학연구원 2006 한국한의학연구원 연구보고서 Vol.- No.-
The purpose of this study was to examine the effects of the Hominis placenta extracts and Astragali radix extracts on improvement of senile osteoporosis (Type 2) in SAM P6. Placenta ex-tracts (Beuronel<SUP>®</SUP>) produced by INBIONET Corp, Daejeon, Korea is hydrolysate of dried human placenta that contains interferon, prostaglandin E1, lysozyme, glycosaminoglycans (GAG) and various amino acid such as leusine, alanine and glutamic acid. In traditional oriental medicine, Placenta (Jahageo) is indicated for insufficiency of blood and tidal fever due to steaming bone disorder. At 10 weeks after birth, Placenta ex. and Astrali Radix ex. was given 100μL and 200μL/kg/day (i.p.), 3times a week for 4 weeks in SAM P6 mice. We measured complete blood cells (CBC) such as RBC, HGB, Hct, PLT, MPB and MCHC. And we analyzed the plasma concentrations of blood urea nitrogen, creatinine, inorganic phosphate and total iron. In addition, we tested bone mineral density (BMD) using the soft X-ray (Lunar Pixirnus). As a result, in SAM P6, injection of Placenta ex. (200μL) increased in RBC, HB and PLT, in comparison with control group. It was also found that the inorganic phosphate levels increased significantly in the injection groups of the Placenta ex. from that of the control group, but blood urea nitrogen was no significant. Moreover, Placenta ex. and Astrali Radix ex. were showed a trend of increase in bone mineral density (BMD) of the vertebrae lumbales, femur and tibia in P6 mice (Fig. 1). These findings suggest that Placenta ex. and Astragali Radix ex. are effective in preventing bone loss in SAMP6. In conclusion, these extracts help on improvement of osteoporosis in SAMs through probably hematogenous functions. <그림참조>
유영법,마진열,하혜경,황대선,김복규,신광수,신현규,Yu, Young-Beob,Ma, Jin-Yeul,Ha, Hye-Kyung,Huang, Dae-Sun,Kim, Bok-Kyu,Shin, Kwang-Soo,Shin, Hyun-Kyoo 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
Objectives: This study presents observation of microorganism such as total aerobic bacteria, total fungus, E. coli, Pseudonomas aerugjnosa, Staphylococcus aureus, and Salmonella typhimurium in herbal decoction manufactured by Korean medical clinic. Methods: We examined to observe microorganism using the requirements for the experimental methods recommended by FDA. For the identification, we observed microscopic methods and carried out polymerase chain reaction (PCR) and DNA purification. The purified DNA samples were analyzed by DNA sequencer. As compared with NCBI database. the results were identified by sequences similarity. Results and conclusion: 26 (55%) of 46 decoctions observed positive for microbial test. 12 (46 %) of 26 positive decoctions exceed requirement of microbial limit test. These microbial colony identified genus of Bacillus using microscopic and DNA sequencing methods.
ICR 마우스를 이용하여 사물탕(四物湯)의 급성 독성에 관한 연구
마진열,유영법,하혜경,황대선,마충제,신현규,Ma, Jin-Yeul,Yu, Young-Beob,Ha, Hye-Kyung,Huang, Dae-Sun,Ma, Choong-Je,Shin, Hyun-Kyoo 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
Objectives: Samul-tang(Siwu-tang) has been traditionally prescribed a medicine as a restorative. Methods: In this study, we investigated the acute toxicity about water-extracted Samul-tang(Siwu-tang). Twenty-five mice completed 14 days of oral Samul-tang(Siwu-tang) at the respective doses of 0(control group), 2560, 3200, 4000 and 5000mg/kg. Results: We observed survival rates, general toxicity, change of body weight, and autopsy. Conclusions: Compared with the control group, we could not find any toxic alteration in all treated groups (2560, 3200, 4000 and 5000mg/kg). $LD_{50}$ of Samul-tang(Siwu-tang) was over 5000mg/kg and it is very safe to ICR mice.
마충제,이남헌,마진열,하혜경,유영법,신현규,Ma, Choong-Je,Lee, Nam-Hun,Ma, Jin-Yeul,Ha, Hye-Kyung,Yu, Young-Beob,Shin, Hyun-Kyoo 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
Objective : The purpose of this report was to provide the information activity and safety of Palmul-tang by analyzing domestic/international papers and theses about Palmul-tang, Methods: Domestic/international papers and theses related to Palmul-tang were reviewed and analyzed, These papers were then classified by year, experimental method and subject, Results: The following results were obtained in this study. 1. The study of Palmul-tang started from 1985 and was continuously increased. The study was mainly forcused on experimental model rather than clinical study. 2. As these studies were classified by subject, papers related to immune intensification were most abundant by 20 papers, Besides there were several papers related to cardiovascular activity, reproductive activity, anti-apoptotic activity and cerebral hemodynamics. 3. Among the papers related to immune intensification. the studies on recovery of fatigue were mostabundant by 10 papers and the studies of on immune cell and cytokine express were six. In addition to. several studies were associated with anti-cancer activity and anti-allergic activity. Recovery of fatigue was determined by measurement of fatigue markers in a living body such as lactate. CPK, pyruvate and triglyceride and assessment of exercise capability of animals such as swimming test. slopped plate test. Rota-rod test, and activity cage test after Palmul-tang treatment. 4. According to experimental data. it is supported that Palmul-tang has been used as Qi and Blood intensifier with immune intensification and recovery of fatigue. 5. The paper related to safety of Palmul-tang was only one paper which is studied on acute toxicity of Palmul-tang with experiment with ICR mouse. There was no study on evaluating safety by observing liver and kidney functions after Palmul-tang treatment Conclusion: Palmul-tang is being used in various ways associating with immune intensification. cardiovascular activity and reproductive activity. However. studies on efficacy and mechanism of Palmul-tang should be conducted at the molecular biology level and studies on safety of Palmul-tang need to be completed at the clinical level.
Chiness hamster lung cell(CHL)에서 십전대보탕 염색체 이상 시험
마진열,황대선,이남헌,하혜경,유영법,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Lee, Nam-Hun,Ha, Hye-Kyung,Yu, Young-Beob,Shin, Hyun-Kyoo 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.2
Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by Chromosomalanomaly test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo Chromosomalanomaly test was performed using chiness hamster lung cell line. Results : Sipjeondaebo-tang water extract was negative in Chromosomalanomaly test at the doses of 0, 625, 1250 and $2500{\mu}g/m\ell$ at 6h and 24h.(S9- fraction). Chromosomalanomaly test(S+fraction) was also negative at the doses of 0, 1250, 2500 and $5000{\mu}g/m\ell$. Conclusions : It was concluded that Sipjeondaebo-tang extract did not induce Chromosomalanomaly in the chiness hamster lung cell.
마진열,이남헌,황대선,하혜경,유영법,신현규,Ma, Jin-Yeul,Lee, Nam-Hun,Huang, Dae-Sun,Ha, Hye-Kyung,Yu, Young-Beob,Shin, Hyun-Kyoo 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.1
Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by micronucleus test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo micronucleus test was performed using 7 weeks ICR mice. At 24 hours after with Sipjeondaebo-tang extract at the doses of 0, 500, 1000 and 2000 mg/kg/day by peritoneal route mice were sacrificed and bone marrow cells were prepared for smear slides. Results : As a result of counting the micronucleus polychromate erythrocyte of 2000 polychromate erythrocyte, all treatment groups did not show statistically significant increase than negative control group. and there was no clinical sign and body weight connected with injection of Sipjeondaebo-tang(Shiquan dabu-decoction) extract. Conclusions: It was concluded that Sipjeondaebo-tang extract did not induce micronucleus in the bone marrow cells of ICR mice
SD 랫트를 이용하여 四君子湯의 아급성 독성에 관한 연구
마진열(Ma Jin-Yeul),유영법(Yu Young-Beob),하혜경(Ha Hye-Kyung),황대선(Huang Dae-Sun),신현규(Shin Hyun-Kyoo) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.3
Sagunja-tang(Sijunzi-tang) has been traditionally prescribed as a restorative. The present study was undertaken to determine the possible toxic effects of Sagunja-tang on SD rats. In this study, we investigated the subacute toxicity of water-extracted Sagunja-tang(Sijunzi-tang) on SD rats. Twenty rats were orally adiministered Sagunja-tang for 28 days at a dose of 0 ㎎/㎏(control group) or 1500㎎/㎏(treated group), respectively. All of subjects was survived. No significant difference in abnormal clinical signs, related to hematological values, serum biochemical values, water and feed intake, coagulation time, autopsy analysis, organ weight, tissue microscopically, funduscopy, urine intake and urinalysis, was detected. Compared with the control group, we could not find any subacute toxic alteration in treated group (l500㎎/㎏) for 28 days. This result suggests that Sagunja-tang(Sijunzi-tang), a herbal medicine prescription, is a safe prescription to body.