간호사의 임상실무지침서 사용현황과 근거중심 임상실무지침서 요구도 조사

하미숙(Ha Mi Suk),박명화(Park Myong Hwa) 한국성인간호학회 2006 성인간호학회지 Vol.18 No.4

Cyclosporine A 투여시 사람 치은섬유모세포에서 IL-6 발현 증가를 유도하는 신호전달경로에 관한 연구

성진효(Jin Hyo Seong),김동기(Dong Kie Kim),하미숙(Mi Suk Ha) 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.1

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치과위생사의 스켈링 작업 시 발현되는 두경부 및 견부 주위 근육들의 표면 근전도를 이용한 특성 분석

전은숙 ( Eun Suk Jeon ),남건우 ( Kun Woo Nam ),하미숙 ( Mi Sook Ha ) 한국치위생과학회 2012 치위생과학회지 Vol.12 No.4

The current research examines the aching part and the muscle activity that happens during scaling practice subject to 20 dental hygienic students and therefore propose a basic data according to the working attitude of the Dental Hygienist. During the scaling we used the free EMG (BTS Inc., Milan, Italy) to measure the muscle activity, and in order to check the musculoskeletal pain area we used the Nordic-style questionnaire, and the measured research results are as following: At the moment of scaling according to posture the occurrence of the pain showed no difference among the groups elbow, back, leg, knee, ankle/foot but in the neck, shoulder, wrist/hand, waist showed a high difference in the degree of pain according to posture. Results from measuring the muscular activity according to posture, in the group that had a good posture, the trapezius and brachial muscles showed a change according to time, and in the group that had a bad posture a high muscle activity was shown in larynx, trapezius and brachial muscles. In the muscle activity, the changing aspect between the two parts, the group that fulfilled a scaling in a good posture showed a low muscle activity, but in the group that fulfilled the scaling in a bad posture the muscle activity increased excessively. Therefore we examined that when fulfilling a scaling maintaining a bad posture can help in using more effectively the activity of the muscle, in the future I think there is a need to raise the office efficiency by subjecting to dental hyginiest that are in the clinics and performing experiments.

Aspergillus awamori, B-2의 균사체 고정화 의한 Melanoidin 탈색

류병호(Beung-Ho Ryu),김혜성(Hye-Sung Kim),하미숙(Mi-Suk Ha),정종순(Jong-Sun Jung),빈재훈(Jae-Hun Bin),이영숙(Young-Sook Lee),정수자(Soo-Ja Chung) 한국식품영양과학회 1989 한국식품영양과학회지 Vol.18 No.1

곰팡중에서 melanoidin의 탈색력이 있는 Aspergillus awamori, B-2을 선별하여 탈색력을 실험하였다. Aspergillus awamori, B-2는 3.0% glucose, 0.5% yeast extract, 0.1% KH₂PO₄ 및 0.05% MgSO₄ㆍ7H₂O을 함유하는 melanoidin 용액(pH 7.0) 에서 5일 동안 37℃에서 배양했을 때 가장 높은 탈색력을 나타내었다. 탈색력을 효과적으로 하기 위하여 Ca - alginate로 고정화하여 최적조건에서 계속적으로 탈색하였을대 mycelia 단독보다는 더욱 효과적이었다. Melanoidin 탈색력은 최적 조건에서 10일 동안 반응하였을때 약 70%을 나타내었다. 고정화한 mycelia의 재사용에 의한 계속적인 탈색을 시도하였던바 15일 동안 연속적으로 반응시켜도 약 60-70%의 탈색력을 계속 유지하였다. Aspergillus awamori, B-2 which has a high ability to decolorize melanoidin was selected among various fungi.<br/> Aspergillus awamori, B-2 showed the highest decolorization activity when it was cultivated in a melanoidin medium containing 3.0% glucose, 0.5% yeast extract, 0.1% KH₂PO₄and 0.05 % MgSO₄ㆍ7H₂O at an initial pH 7.0 at 37℃ for 5 days.<br/> Mycelia immobilized system with Ca-alginate was more effective on melanoidin decolorization activity showed approximately 70% in 10 days under the optimal conditions.<br/> Continuous decolorzation of melanoidin using reuse of immobilized mycelia showed an almost constant decolorization of abort 60-70 % for 15 days.

연령의 증가에 따라 동화구연과 동화활동이 유아의 동화기억 및 이해에 미치는 영향

하미숙 한국아동교육학회 1996 아동교육 Vol.5 No.2

This study is aimed at understanding how best children between the ages three and five comprehend stories. Specifically, this study measures the effectiveness between two seperate ways of story comprehension one by simply reading the stories to the children and the other by having the students act out the story as in plays. 46 three-years-olds, 51 four-years-olds, and 63 five-years-olds served as the subjects of this study over two weeks. Three stories that the subjects had not heard or read were chosen for this study. The students were divided into two groups. (1)those who were simply read to and (2)those who acted out those stories. The second group was further divided by sex. After conducting the story telling workshop a total of eighteen questions, which were developed earlier, were asked to the students. The results were calculated for the compression scores. Anova was conducted analyze how the members of the three said groups understood the story. The result of the analysis: 1. On the simple memory performance, dramatic enactment was more effective than story-reading for age 3. but there was no significant differnce between age 4 and age 5. 2. On the story comprehension, dramatic enactment was more effective than story-reading for age 3 but there was no significant difference for age 4 and age 5. 3. On the overall compression test, drama group showed higher score than story-reading group for age 3. For age 4 and age 5, there was no significant difference. 4. On the comparison of children's age, there was some difference between story-reading and enactment of the stories for Age 3. but there was no difference for age 4 and age 5. S. There was no significant difference for boys and girls of all ages on the test. 6. There was an interaction between by story and by sex for age 3, age 4, and the comprehension of age 5. and also There was an interaction for simple-memory and overall compression of age 5.

洛東江 水質의 變化

李宗哲,河美淑,金東奭 慶星大學校 1986 부산수산대학 논문집 Vol.7 No.2

Nakdong River goes through the south-east area of the Korean Peninsula, and make up a kim hae delta and finally flows into the south sea of Korea. It is very important as the source of irrigational, industrial and portable water according to development of industry and increase of population. This study is designed to investigation which control of ecological changes result from the construction of river estuarine barrage. As the first part of that, we tried to check the water qualities during spring season. Water temperature changed from 12.4 to 21.5℃. The pH variation of the samples was ranged 6.1∼8.0 which showed significantly broad spectrum. The concentration of salinity was between 0.02 and 30.90‰ and increased by effect of the sea water along the down stream. The dissolved oxygen was ranged 5.6∼8.9 mg/ℓ and lower part of the Nakdong River was more contaminated by domestic factories area, The values of biochemical oxygen demand varied from 1.5 to 13.6 mg/ℓ and the range of values of chemical oxygen demand 3.1∼15.0 mg/ℓ The values of nutrients varied from 18 to 280 ㎍-N/ℓ for NO₂N, from 63 to 932 ㎍-N/ℓ for NO₃-N form 64 to 1032 ㎍-N/ℓ NH₄-N, form 18.3 to 52.0 ㎍-P/ℓ for phosphate from 1.0 to 4.3 mg/ℓ for silicate.

In vitro yeast 2㎛ plasmid DNA replication by cell division cycle genes

Ryu, Beong-Ho,Ha, Mi-Suck,Kim, Dong-Suk 慶星大學校 1986 부산수산대학 논문집 Vol.7 No.2

Saccharomyces cerevisiae의 extrachromosomal 2㎛ plasmid DNA의 복제기구를 밝혀볼 目的으로 cell cycle의 突然變異菌株을 이용하여 실험하였다. cdc 突然變異菌株는 DNA의 복제를 야기할 수 있는 可能한 溫度는 23℃였다. 그러나 cdc mutants를 36℃에서 한 세대동안 배양했을 때는 그 抽出物에 있어서는 活性이 매우 낮거나 거의 찾아볼 수 없었다. 결국 모든 突然變異原菌株는 酵母의 cell cycle에 있어서 DNA 합성의 開始를 유도하는 의존 과정에서는 불충분하였다. The replication of the 2 ㎛ plasmid DNA of Saccharomyces cerevisiae has been examined in cell division cycle(cdc) mutants. Temperature sensitive mutant of the cell(cdc) grown at permissive temperature (23℃) yielded extracts that were capable of stimulating DNA replication. When the cells were incubated for one generation at the nonpermissive temperature (36℃), their extracts showed very low or no activity. All of these mutants are deficient in events of the dependent pathway leading ot initiation of DNA synthesis in the yeast cell cycle.

Effects of Radical Scavengers and Antioxidants on HgCl_(2)-Induced Cell Injury in Human Glioma Cells

Cho, Yong Woon,Ha, Mi Suk,Woo, Jae Suk,Jung, Jin Sup,Kim, Yong Keun 고신대학교 의학부 2001 高神大學校 醫學部 論文集 Vol.16 No.1

배경 수은은 신경조직에서 여러 가지 독성을 나타내며 알츠하이머병의 병태생리학적 기전에 관련되어 있는 것으로 알려져 있다. 생체 및 시험관 내 실험을 통하여 신경을 포함한 여러 조직세포에서 수은이 반응성 산소기를 발생시키고 지질의 과산화를 유발하는 것으로 밝혀짐으로써 산화성 스트레스가 수은에 의해 유발되는 여러 가지 신경성 질환에 관여할 것으로 예상된다. 그러나 지질의 과산화가 수은에 의한 세포독성에 중요한 역할을 하는지에 대해서는 분명하지 않다. 방법 본 연구에서는 수은에 의한 세포손상에 어떤 반응성 산소기가 관여하며, 지질의 과산화가 수은에 의한 세포독성에 기여하고 있는지를 인간 신경교종세포인 A172 세포를 이용하여 조사하고자 하였다. 결과 수은은 농도와 처리시간에 비례하여 세포사망을 일으다. 이러한 변화는 Hydroxyl radical scavenger인 dimethylthiourea에 의해 방지되었으나, superoxide scavenger인 superoxide dismutase와 hydrogen peroxide scavenger인 catalase와 pyruvate에 의해서는 영향을 받지 않았다. 황산화제인 DPPD, Trolox 및 melatonin은 방지효과를 나타내지 못했다. 수은에 의한 세포손상은 iron chelator인 phenanthroline과 sulfhydryl group 방지제인 dithiothreitol과 glutathione에 의해 방지되었다. 수은은 세포 내 ATP농도를 감소시켰으며, 이러한 효과는 수은에 의한 세포손상을 방지하는 약물들에 의해 억제되었다. 결론 철 이온에 의존적인 기전을 통해 발생된 hydroxyl radical이 수은에 의한 세포손상에 관련되어 있으며, 지질의 과산화는 수은에 의한 세포독성에 중요한 역할을 하지 못하는 것으로 생각된다. Background Mercury exerts a variety of toxic effects on both neurons and glia and also may play a role in pathophysiological mechanisms of Alzheimer's disease in neuroblastoma cells. Studied in vivo and in vitro have shown that mercury generates reactive oxygen species(ROS) and increase lipid peroxidation in various tissues including brain, suggesting that oxidative stress may contribute to the development of neurodegenrative disorders caused by mercury intoxication. However, whether lipid peroxidation plays an important role in mercury cytotoxicity is not clear. Methods The present study was undertaken to determine, (1) the involvement of individual ROS in mediating mercury cytotoxicity and (2) whether mercury-induced cell death is resulted from lipid peroxidation in human glioma cells. HgCl_(2) caused the loss of cell viability in a dose-and time-dependent manner. Results The loss of viability was prevented by the hydroxyl radical scavenger dimethylthiourea, but the superoxide scavenger superoxide dismutase and the hydrogen peroxide scavengers catalase and pyruvate showed no beneficial effect. The potent antioxidant DPPD exerted partial protective effect, but BHA. Trolox, and melatonin were not effective HgCl_(2)-induced loss of viability was prevented by the ferrous iron chelator phenanthroline, but no the ferric iron chelator deferoxamine HgCl_(2) cytotoxicity was effectively prevented by thiols (dithiothreitol and glutathione). HgCl_(2) caused ATP depletion, which was prevented by the agents protecting HgCl_(2)-induced cell death. Conclusion These results suggest that (1) HgCl_(2)-induced cell death is associated with generation of hydroxyl radicals resulting from and iron-dependent mechanism and (2) lipid peroxidation dose not play a critical role in HgCl_(2) cytotoxicity in human glioma cells.

Cyclosporine A 투여시 사람 치은섬유모세포에서 IL-6 발현 증가를 유도하는 신호전달경로에 관한 연구

성진효,김동기,하미숙 朝鮮大學校 口腔生物學硏究所 2002 口腔生物學硏究 Vol.26 No.1

Cyclosporine A(CsA) is a widely used immunosuppressant, with clinical applications ranging from organ transplants to chronic inflammatory diseases. One of the side effects associated with CsA treatment is the development of gingival overgrowth. Alghough CsA has been shown to stimulate IL-6 production in human gingiva fibroblast cells in culture, the signaling mechanism that regulate IL-6 production is not fully understood. The aim of this study was to examine the role of the p38 and extracelluar signal-regulated kinase(ERK) mitogen-activated protein kinase (MAPK) pathway in regulating CsA mediated IL-6 production from human gingiva fibroblast.Human gigiva fibroblast cultures were established from systemically healthy gingival tissue donors. Cells were treated for various time with CsA in the presence and absence of the specific p38 and ERK1,2 MAPK inhibitors SB203580 and PD98059. respectively, either untreated or treated. IL-6 levels in the cell culture media were measured b enzyme-linked immnosorbent assays. MAPK activation was demonstrated by immunoblot for the active kinase (tyrosing/threonine phosphorylated) in whole cell extracts using phospho-specific antibodies. p38 MAPK activity in human gingival fibroblast cells was measured using an in vitro immunokinase assay using ATP as production in human gingival fibroblast. The activation of the p38 and the ERK1.2MAPKs occured following CsA stimulation. Pretreatment with various inhibitors had effect on CsA stimulated p38 MAPK activity. PD98059 decreased CsA stimulated phosphorylation of ERK1.2. Additionally, Wortmannin and LY290042, a potent inhibitor of the PI3K-kinase were able to inhibit IL-6 activation, but they had no effect on IL-6 activation when stimulated by CsA. This study provides evidence that both the p38 and ERK MAPK pathways are important for the regulation of the production of IL-6 from the human gingival fibroblast in response to CsA.