말똥 성게의 DNA Polymerase α 의 정제와 특성

하미숙,류병호 한국수산학회 1987 한국수산과학회지 Vol.20 No.2

말똥 성게를 인공 수정시킨 후 column chromatography 법으로 DNA polymerase α를 분리 정제하였다. Sephadex G-200과 SDS polyacryamide gel electophoresis에 의한 DNA polymerase α의 분자량은 약 137,000∼138,000이였다. 효소활성의 최적 pH는 7.4였고, 칼슘이온 20mM, 나트륨이온 25mM에서 활성이 높았고, 마그네슘 이온은 10mM일 때 활성이 높았다. DNA polymerase α는 N-ethylmaleimide, aphidicolin, cytosin β-D-arabinofuranoside 5′-triphosphate (ara CTP)와 phosphonoacetic acid에 의하여 활성이 크게 저하되었다. From the sea-urchin, Hemicentrotus pulcherrismus, we have purified by four column chromatographic steps for DNA polymerase α activity. The molecular weight of DNA polymerase α was determined to be around 137,000-138,000 by Sephadex G-200 gel filtration and SDS-polyacrylamide gel electrophoresis. The purified enzyme had the optimal activity at pH 7.4. This enzyme showed to be a function of the metal ion K^+, Na^+ and Mg^(2+) employed as activators, the optimum K^+ or Na^+ concentration were 20mM or 25mM and the optimum Mg^(2+) concentration was 10mM. The enzyme activity was inhibited by N-ethyl-maleimide, aphidicolin, cytosine β-D-arabinofuranoside 5′-triphoshate (ara CTP) and phosphonoacetic acid.

상대적 운동강도가 척수앞뿔 손상쥐에서 NT-4/5와 IL-6의 변화에 미치는 효과

하미숙,한동욱 한국자료분석학회 2009 Journal of the Korean Data Analysis Society Vol.11 No.5

This study was designed to investigate the effects of swimming on motor function recovery and hematological assay after Spinal Cord Injury(SCI) rats. SCI model rats were damaged in L1-L2 injected with 6-OHDA. After operation, rats were tested at neurological motor behavior test(Modified inclined plane test, Rota-rod test) at 1, 4, 7, 10, 14 days and EIA(IL-6, NT-4/5) at 14 days. Behavior test, experimental group were difference between control group. At 14 days, there were significantly higher in the II group than the control group. Concentration of IL-6, experimental group were decreased between control group, but this result were not significant. Concentration of NT-4/5, II group were significantly increased between control group. Especially, low-intensity exercise inhibition of second damage of spinal cord and facilitation of locomotion recovery and expression of NT-4/5. 본 연구는 척수앞뿔의 세포손상을 준 흰쥐를 대상으로 운동강도를 달리한 수영운동을 통해 운동기능과 신경계의 회복을 알아보는데 목적이 있다. 본 연구의 진행은 SD계 흰쥐의 허리뼈 1-2번 사이에 6-OHDA로 척수손상을 일으킨 다음 3일 후에 치료를 시작하였다. 수영은 저강도수영군과 고강도수영군으로 나누고 주 5회 15분간 운동을 실시하고, 치료를 시작하기 전에 운동기능의 평가를 시행하며 2주 동안 4번의 검사가 이루어졌다. 혈청 IL-6, NT-4/5에 관한 측정은 2주간 치료 후 검사하였다. 수정된 경사판 검사와 로타로드 검사에서 대조군에 비해 수영군에서 차이가 있었으며, 고강도수영군에 비해 저강도수영군에서 증가되는 폭이 컸다. IL-6 검사에서 대조군에 비해 수영군에서 감소되었으나 유의한 차이는 없었다. NT-4/5는 대조군에 비해 저강도수영군에서 증가하였다. 결과적으로 저강도운동은 척수손상 환자에게 효과적인 치료방법이 될 수 있으며, 장애를 최소화하여 자발적인 일상생활을 도모할 수 있을 것이라 생각된다.

양측성 부하가 보행의 운동학적 요인에 미치는 영향

하미숙,남건우,Ha, Mi-Sook,Nam, Kun-Woo 대한물리치료과학회 2013 대한물리치료과학회지 Vol.20 No.1

Background : The purpose of this study was to investigate the effects of the kinesiologic factors of gait on symmetric load. Methods : The subjects were consisted normal 33 persons (10 males and 23 females). The kinds of weight of the bag was 0kg, 5kg and 7kg. The kinesiologic factors of gait measured by three dimensional motion analysis system and callibration marker. Callibration was ASIS, hip greater trochanter, knee lateral epicondyle on sagittal plane, ankle lateral malleolus on sagittal plane, toe 5th phalange. The changes kinesiologic factor were analyzed using one way ANOVA with SPSS 21.0 package program. Results : The weight of the bag was statistical significance on change of hip joint and knee joint(p<.05). The weight of the bag was no significance on change of ankle joint(p>.05). The right and left of the lower limbs was no significant(p>.05). Conclusion : This research provides weight of bag for the gait. This study showed that symmetric load does affect kinesiologic factors of gait. This indicates that there is an interaction that plays a crucial roles in the weight of bag and kinesiologic factors of gait.

중추신경계 손상시 GFAP의 발현에 대한 고찰

하미숙,박수경,박래준 대구대학교 특수교육재활과학연구소 2007 再活科學硏究 Vol.25 No.1

이 연구는 중추신경 손상에 대한 문헌적 고찰을 통해 아교 섬유성 산 단백질(glial fibrillary acidic protein : GFAP)에 대한 이해와 경과 시간에 따른 발현시기 등을 알아보고자 한다. GFAP는 별아교세포에만 특이적으로 염색되어 별아교세포의 특이적 표지물질(specific marker)로 사용되어 지고 있다. GFAP의 발현정도가 손상 후 1주일째까지 가장 현저히 증가하는 것으로 보아 손상 초기에 물리치료적 중재를 통해 중추신경계의 기능회복에 있어서 좋은 결과를 얻을 수 있다. Purpose : The purpose of this study was to uderstand the glial fibrillary acidic protein through literature review about CNS injury. Method : This is a western bolt analysis, immunocytochemical analysis and double immunofluorescence for glial fibrillary acidic protein were performed after CNS injury. Result : The GFAP expression level in the gray matter increased in the early stage of injury. In contrast the GFAP expressoin level in the gray matter decreased in the late stage of injury. Conclusion : The study of GFAP is the intermediate filament of the glial cells and useful as a marker for the astrocytes. Also, the upregulation of GFAP espression required various physical therapeutic approaches.

운동프로그램이폐기능및보행거리에미치는영향

하미숙 한국지역사회건강관리협회 대한건강과학학회 2020 대한건강과학학회지 Vol.17 No.2

Background: The purpose of this study is figure out some change of lung function and walking distance by exercises program in health adults. Method: The all subjects are healthy and participated voluntary We had divided 2 group (10 people to compare group, 10 people for the gym ball exercise group) and the effects on lung function were measured with digital spirometry and walking distance with six-minute walk test. And the t-test was conducted to analyze among the compared group, the gym ball exercise group the differences between before and after the experiment. Result: Gym ball exercise group was a significant difference in the FEV1, FEV1/FVC%, FEF25 - 75%(p<0.05) between before and after exercises. The control group, however, showed no change(p>0.05). Conclusion: This study demonstrated that gym ball exercise could be helpful for lung function. Various exercises in future research on intervention programs can be studied to promote the public if the functional status is considered to be of much help.

육류중 장기의 유기염소계 잔류농약

하미숙,류병호,이종철 한국식품위생안전성학회 1986 한국식품위생안전성학회지 Vol.1 No.2

n beef, pork, chicken-flesh, and goat-flesh sampled from Pusan area, residues of various kinds of organochlorine pesticides were examined. The residues of organochlorine pesticides in the all of samples were found to be in the order of α-BHC, ρ-BHC, heptachlor epoxide PP'-DDE, and m-BHC. The residues of organochlorine pesticides such as heptachlor, PP'-DDE and PP'-DDD were comparatively in low level or trace and drins-pesticides were not detected in all of samples. The average concentrations of total BHC in beef pork, chicken and goat were found 0. 713 ppb, 0. 673 ppb, 0.517 ppb and 0. 586 ppb. Residue contents were found in the order of beef, pork, goat and chicken. The highest concentration was found in the adipose tissue.

호흡운동이 호흡근 활성도 및 흉곽용적에 미치는 영향

하미숙,남건우,Ha, Mi-Sook,Nam, Kun-Woo 대한물리치료과학회 2014 대한물리치료과학회지 Vol.21 No.1

Background : This study is for respiratory muscle activity and chest expansion through practice abdominal breathing exercises. Methods : The subjects were consisted normal 30 persons(15 males and 15 females). The control group to 15 people to compare group and 15 people for the abdominal breathing exercise group through lip retraction movement of the therapist with the resistance of mediated abdominal breathing exercises. For 8 weeks EMG was used to know the changes in respiratory muscle. We also found out the changes in chest expansion. And the t-test was conducted to analyze among the compared group, the abdominal breathing group the differences between before and after the experiment. Results : On the changes in respiratory muscle muscular activity in the transverse abdominis have shown significant results(p<.05), and the change in chest expansion was no significant(p>.05). Conclusion : Various breathing exercises in future research on intervention programs can be studied to promote the public if the functional status is considered to be of much help.

척수전각손상 흰쥐에서 전침자극이 운동기능 회복에 미치는 영향

하미숙,노민희,박래준,김은영,박수경 한국스포츠리서치 2007 한국 스포츠 리서치 Vol.18 No.6

습관화 운동이 어지름증과 주관적 시각 수직감에 미치는 영향

하미숙 한국심리협회 2009 심리행동연구 Vol.1 No.1

본 연구의 목적은 습관화 운동이 어지름증과 주관적 시각 수직감에 미치는 영향을 알아보는 데 있다. 본 연구의 대상은 실험집단(남:N=8, 여:N=7)과 통제집단(남:N=8, 여:N=7)의 각각 15명씩 총 30명 이었다. 주관적 시각 수직감 측정과 전기안진검사를 1차적으로 실시한 후 실험집단에게만 3주 동안 매일 3회씩 습관화 운동을 수행하도록 하였다. 3주 후에 실험집단을 대상으로 주관적 시각 수직감을 2차 검사하여 그 변화를 살펴보았다. 실험집단에게서 좌측, 우측, 주관적 시각 수직감 영역에 있어 통계학적으로 유의미한 변화를 보였다. 본 연구 결과 습관화 운동이 어지름증과 주관적 시각 수직감에 많은 영향을 미치는 것으로 나타났다.

기능적 발목불안정시 하지 근피로가 균형에 미치는 영향

하미숙 한국지역사회건강관리협회 대한건강과학학회 2017 대한건강과학학회지 Vol.14 No.1

Background : The purpose of this study was to investigate the effects to the balance of functional ankle instability(FAI) subject. Method : Subject in this study consisted of students 36people, using the Cumberland ankle instability tool(CAIT) was selected as FAI by 24points or less. The group was classified under two groups, ankle joint stable group, ankle joint unstable group consist of 18 persons each. Result : Balance among the two groups was not significantly different(p>.05). Conclusion : Based on these results, balance of ankle joint unstable group was the smallest average value. Therefore, therapists and sport trainers could still minimize the risks of fall and injury respectively related to muscle fatigue.