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난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 I. 개 보데텔라, 파보바이러스 및 개디스템퍼 항원의 닭에서의 면역반응
이희수,김종만,우승룡,정병열,조윤상,탁동섭,임숙경,유한상,윤용덕,허원,문영식,오진식,Lee, Hee-soo,Kim, Jong-man,Woo, Seng-ryong,Jung, Byeong-yeal,Cho, Yun-Sang,Tark, Dong-seob,Lim, Sook-kyoung,Yoo, Han sang,Yoon, Yong-dhuk,Huh, Woo,Mun, Y 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1
This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280 ~ 1:10,240 in sera and 1:160 ~ 1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80 ~ 1:320 and 1:64 ~ 1:256, respectively. Virus neutralization titers against canine distemper was 1:8 ~ 1:64 in sera and egg yolk. These results suggested that egg yolk antibody titers could be variable according to a sort of adjuvant and antigens of the pathogens.
가축 전염병 관리를 위한 돼지 사체의 화학적 처리 방법
오연수 ( Yeonsu Oh ),윤석훈 ( Suk Hoon Yoon ),탁동섭 ( Dong-seob Tark ),조호성 ( Ho-seong Cho ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.4
This is a study on the improvement of the chemical treatment method of the livestock carcass treatment newly introduced in the livestock infectious disease prevention method in order to improve the problems of the existing burial-centered carcass treatment method when a livestock infectious disease occurs. It was conducted to establish detailed treatment standards for the chemical treatment method of pig carcasses based on the results of proof of the absence of infectious diseases in pigs. After inoculating pig carcasses with 10 pathogens (6 viruses [FMDV, ASFV, CSFV, PCV2, PRRSV, PEDV] and 4 bacteria [Lawsonia intracellularis, Clostridium perfringens type C, E. coli , Salmonella Typhimurium]) It was treated at 90℃ for 5 hours in a potassium hydroxide (KOH) liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens. Based on these results, it was possible to prove that chemical treatment of pig carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. Although there are problems to be solved in the processing and operation of the chemical treatment products of livestock carcasses, the chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.
원저 : 사슴 재조합 프리온 단백질의 발현,정제 및 특성 조사
김효진 ( Hyo Jin Kim ),이윤희 ( Yoon Hee Lee ),정혜영 ( Hye Young Jeoung ),김민정 ( Min Jeong Kim ),탁동섭 ( Dong Seob Tark ),조인수 ( In Soo Cho ),손현주 ( Hyun Joo Sohn ) 한국수의공중보건학회 2010 예방수의학회지 Vol.34 No.4
Abnormal prions are infectious agents involved in a neuro-degenerative disease, which occurs naturally such as Chronic Wasting Disease (CWD) in deer and elk, Bovine Spongiform Encephalopathy (BSE) in cattle, Scrapie in sheep and goats and Creutzfeldt-Jakob Disease (CJD) in humans. The cellular prion protein of the elk consists of 233 amino acids (residues 25-257), which represents an autonomous folding unit with three α-helices and two-stranded anti-parallel β-sheets. Here, we demonstrated elk-recPrP (Elk recombinant prion protein) which can be obtained as follows; (1) Cloning of elk PrP gene, (2) Expression of a histidine-tagged full-length elk PrP by induction with IPTG in E. coli and (3) Purification by affinity chromatography using Ni-NTA agarose resin. In Western blot and ELISA analysis, elk-recPrP showed specific activity against anti-PrP monoclonal antibody. Thus, our elk-recPrP would be a useful tool for the understanding of basic structure and mechanism studies of PrPSC formation.