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해양 현장증거물의 접촉흔 탐지를 위한 nucleic acid fluorescence 적용
신민섭(Min Seop Shin),김남율(Nam Yul Kim),추민규(Min Kyu Choo),이한성(Han Seong Lee) 한국해양환경·에너지학회 2021 한국해양환경·에너지학회 학술대회논문집 Vol.2021 No.5
범행도구에 남겨진 혈흔, 미세흔적 및 체액 등은 오랜 시간이 경과해도 DNA 감정을 통해 범죄 사실을 입증할 수 있다. 하지만 범인은 증거물을 유기하기 위하여 수중이나 산속, 수풀 등 환경요인을 이용하고 있으며, 이런 환경은 각종 오염물질과 DNA 훼손 등으로 DNA 감정을 어렵게 하고 있다. 이러한 열악한 환경에서 수집된 증거물은 이화학적 및 생물학적 오염으로 인해 범행 흔적을 찾기가 어려워 DNA를 효과적으로 채취하는 방법에 대한 연구가 필요하다. 본 연구는 DNA 형광 염색 시약을 이용하여 현장(수중) 증거물에 존재하는 잠재 DNA의 시각화 가능성을 제시하고자 한다. 잠재 DNA를 시각화 하는데 가장 효과가 우수하다 보고된 Diamond™ Nucleic Acid Dye를 처리하여 일반 생활용품의 재질 표면에 유류된 타액과 지문의 탐색여부를 확인하였다. 연구결과, 증거물 재질 중 비다공성 표면(유리, PVC, 스테인리스강, 인조가죽, 검정비닐)에서 타액과 지문의 탐색이 가능하였으며, 해양에 침수된 증거물에서도 시약을 처리 시 1 ~ 2일 동안 타액과 지문을 식별할 수 있었다. 또한 DNA를 활용한 신원확인 기법인 STR 분석에 영향을 미치지 않는 것으로 나타났다. 이와 같이 해수에 침지된 증거물에도 접촉흔 탐지 및 DNA 감정이 가능하므로 현장 수사요원들이 더 적극적으로 증거물 수집에 임할 필요가 있다. It is possible to prove the fact of crime through the identification of DNA from bloodstains, micro-traces, and body fluids remaining on the tools of the crime even after much time has passed. However, criminals use environmental factors (water, mountain, and the woods) to dispose of evidence, and these environments make it difficult to identify DNA due to various pollutants and damage to DNA. For evidence collected from these deteriorated environments, it is difficult to find traces of crimes due to physicochemical and biological contamination. Thus, it is necessary to conduct a study of the method of collecting DNA effectively. This study would present the possibility of the visualization of latent DNA existing in the evidence at the site (in underwater evidence), using DNA fluorescent dye reagent. Treating it with Diamond™ Nucleic Acid Dye, reported to be the most excellent in visualizing latent DNA, this study investigated saliva and fingerprints remaining on the surface of the materials of general household items. As a result of the research, it was possible to explore saliva and fingerprints on the non-porous surface (Glass, PVC, stainless steel, artificial leather, and black vinyl) of the materials, and also in the evidence flooded in the sea, it was possible to identify saliva and fingerprints for 1 or 2 days when treated with the reagent. Also, there was no impact on STR analysis, a method of identification utilizing DNA. Since it is also possible to detect contact traces and identify DNA for evidence immersed in seawater, crime scene investigators should collect evidence more actively.
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윤현경,김진선,김세인,김준수,추민규,Yoon, Hyun-Kyoung,Kim, Jin-Sun,Kim, Sea-In,Kim, Jun-Soo,Choo, Min-Kyu 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.10
Humans technological advancements have resulted in the depletion of whale resources. Accordingly, the International Whaling Commission was established to preserve whale resources and ensure the orderly development of the whaling industry. After a commercial whaling moratorium came into effect, the international trade of whale meat and related products was banned. However, There is a systematic activity through illegal remodeling ships because whales incidentally caught may be distributed in Korea and have a significant economic benefit. Although suspected illegal whaling is actively cracked down, but it is still insufficient to prevent illegal whaling and distribution. To prevent this, stereoscopic crackdowns utilizing air forces and patrol ships are effective, and it is necessary to quickly separate the captured ship and crew to prevent the destruction of evidence. For the transparent distribution of whale meat, it is necessary to advance related technologies such as whale species identification and individual identification of forensic science institutions based on whale DNA database of the National Institute of Fisheries Science. Accordingly, the Korea Coast Guard Research Center is directly conducting research on related national R&D project. To increase the efficiency of identifying whale-related evidence at crime scene, a rapid test kit that responds specifically to whale bloodstrains is developing and evidence transport packs are manufacturing and distributing, while identification technologies are also being advanced.
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해양환경 내 비다공성 표면에 유류된 잠재지문 현출방법에 따른 STR 분석 연구
김진선,김세인,윤현경,추민규,Kim, Jin-Sun,Kim, Sea-In,Yoon, Hyun-Kyoung,Choo, Min-kyu 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.10
Among the various evidence found in maritime crimes, fingerprints and DNA are very important in that they can identify a suspect. In this study, 5 types of non-porous surfaces (plastic, stainless, glass, ceramic, FRP), which are often found as evidence in the actual marine environment, were selected, and latent and blood fingerprints were passed down and immersed at the Donghae Maritime Police Station's exclusive pier for about 7 days. After that, DNA extraction, quantification, and STR profile were analyzed after fingerprint developing CA fumming method and 4 powder methods (Swedish black powder, Concentrated black powder, Supranano red powder, Dazzle orange powder). Among the fingerprint developing methods, when Supranano red powder was applied, a relatively high amount of DNA was found. As a result of STR profile analysis, an average of 16.8 to 9 loci were secured, and all 20 were confirmed in glass and ceramic materials. As a result of the study, it was possible to secure the STR profile by extracting and quantifying DNA after applying the fingerprint developing method to virtual evidence immersed for about 7 days, and further research is needed to secure the STR profile by analyzing DNA after applying various fingerprint developing methods such as VMD and SPR.
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