난백에 감작이 있는 2세 미만의 소아에서 난백 특이 IgE 변화 결정 인자

최엄지,백은영,송근백,윤지선,유진호 대한 소아알레르기 호흡기학회 2023 Allergy Asthma & Respiratory Disease Vol.11 No.2

Purpose: Food sensitization to egg white (EW) peaks during early childhood, after which some children with sensitization to EW outgrow food sensitization, while others develop an increased degree of sensitization to EW. We investigated factors determining changes in EW-specific IgE in children under 2 years of age. Methods: Among children under 2 years of age with atopic dermatitis or food allergy who visited Asan Medical Center from March 2018 to September 2021, a total of 172 sensitized to EW were enrolled. Serum specific IgE to EW, ovalbumin and ovomucoid, and specific IgG4 to EW and ovomucoid were measured at 0, 6, and 12 months. An increase in EW-specific IgE was defined as an increased level of EW-specific IgE after 12 months from baseline. Multiple logistic regression analysis was performed to identify factors determining an increase in EW-specific IgE. Results: Of the 172 children, 85 had complete data on serum specific IgE and specific IgG4 during a period of 12 months. The levels of EW-specific IgE were decreased in 51 subjects (60.0%), while they were increased in 34 subjects (40.0%). Introduction of baked eggs had a protective effect against an increase in EW-specific IgE levels during a period of 12 months (adjusted odds ratio, 0.148; 95% confidence interval, 0.022–0.983). Conclusion: Introduction of the baked egg can prevent an increase in EW-specific IgE during early childhood. Further studies are needed for the long-term effect of baked egg on the natural course of EW sensitization. .

Chromomycin A3 생산량이 증진된 Streptomyces sp. SJ1-7 돌연변이 균주 동정

최엄지,이한별,지원재,김순옥,이창로 한국미생물학회 2023 미생물학회지 Vol.59 No.1

Streptomyces sp. SJ1-7, a soli bacteria isolated from the rhizosphere of a Pinus densiflora plant in South Korea, produces an aureolic acid type antibacterial and antitumor compound chromomycin A3 with industrial applications in DNA and cell staining. In this study, we identified chromomycin A3- overproducing mutant strains of Streptomyces sp. SJ1-7 through a high-throughput screening assay. After mutation by exposure to UV irradiation, the production level of chromomycin A3 in mutant strains was determined by measuring inhibition zone diameters of Staphylococcus aureus. We selected 70 mutant strains with stronger antibacterial activity than the wild-type strain. Absorbance spectroscopy and thin layer chromatography analysis showed that the chromomycin A3 levels in several mutant strains were more than 3-fold higher than that in the wild-type strain. Taken together, these results demonstrate the identification of chromomycin A3-overproducing Streptomyces sp. SJ1-7 mutant strains through the high-throughput screening method using UV-induced mutation.

Predictors of High-grade Vesicoureteral Reflux in Children with Febrile Urinary Tract Infections

최엄지,이민주,박신애,이오경 대한소아신장학회 2017 Childhood kidney diseases Vol.21 No.2

Purpose: This study aimed to investigate clinical and radiological factors that may predict high-grade vesicoureteral reflux (VUR) in patients with febrile urinary tract infection (UTI). Methods: We retrospectively analyzed medical records of 446 patients diagnosed with febrile UTI from March 2008 to February 2017. All patients underwent renalbladder ultrasonography (RBUS), 99mTc dimercaptosuccinic acid (DMSA) renal scan, and voiding cystourethrography (VCUG), and were divided in to 3 groups: a high-grade VUR group (n=53), a low-grade VUR group (n=28), and a group without VUR (n=365). Results: The recurrence and non-Escherichia coli infection rates in febrile UTI were significantly higher in the high-grade VUR group than in the other two groups (P <0.05). RBUS showed that hydronephrosis and ureter dilatation were more frequent in the high-grade VUR group than in the other groups (P <0.05). In the high-grade VUR group, a renal cortical defect was more likely to appear as multiple defects, and the difference in bilateral renal scan uptake between both kidneys was larger than in the other two groups (P <0.001). Conclusion: Recurrent UTI, non-E. coli UTI, abnormal findings on RBUS such as hydronephrosis and ureter dilatation, and abnormal findings in the DMSA renal scan such as multiple renal cortical defects and greater uptake difference were associated with high-grade VUR. VCUG should be selectively performed when RBUS and/or DMSA renal scan reveal significant abnormalities.

Streptomyces sp. SJ1-7에 존재하는 PadR 타입 전사조절인자 CmmRII의 생화학적 특징 분석

최엄지,체벨호르로 마랄,지원재,홍순광,이창로 한국미생물학회 2023 미생물학회지 Vol.59 No.1

Chromomycin A3 is an aureolic acid type antibacterial and antitumor compound produced by Streptomyces species. The biosynthetic gene cluster of chromomycin A3 is composed of more than 30 genes involved in polyketide biosynthesis and modification, sugar biosynthesis and transfer, and resistance, but the regulatory mechanisms of their expression are poorly understood. In this study, we analyzed biochemical features of the AfsR-type transcriptional regulator CmmRI and PadR-type transcriptional regulator CmmRII, which are located within the biosynthetic gene cluster of chromomycin A3 of Steptomyces sp. SJ1-7, isolated from soil surrounding the root of a Pinus densiflora plant in Sangju, South Korea. CmmRII interacted with several putative promoter regions of chromomycin A3 biosynthesis genes, whereas CmmRI interacted with no promoters tested. The presence of chromomycin A3 hardly affected the interaction between CmmRII and the promoter region. Purified CmmRII existed in as a monomeric form. These results imply that CmmRII is an important transcriptional factor in expression regulation of the biosynthetic gene cluster of chromomycin A3 in Steptomyces sp. SJ1-7.

탈인산화된 NPr에 의한 대장균의 섬유상 생장

최엄지,석영재,이창로,Choi, Umji,Seok, Yeong-Jae,Lee, Chang-Ro 한국미생물학회 2017 미생물학회지 Vol.53 No.3

The nitrogen phosphotransferase (PTS) system is a regulatory cascade present in most Proteobacteria, where it controls different functions. The nitrogen PTS is usually composed of $EI^{Ntr}$ (encoded by the ptsP gene), NPr (encoded by the ptsO gene), and $EIIA^{Ntr}$ (encoded by the ptsN gene). While $EIIA^{Ntr}$ plays a role in a variety of cellular processes, such as potassium homeostasis, regulation of ppGpp accumulation, nitrogen and carbon metabolisms, and regulation of ABC transporters, little information is available for a physiological role of NPr. A recent study showed that dephosphorylated NPr affects adaptation to envelope stresses in Escherichia coli. In this study, we provide another phenotype related to NPr. The ptsP mutant showed a filamentation phenotype. The filamentation phenotype of the ptsP mutant was recovered by additional deletion of the ptsO gene, but not by additional deletion of the ptsN gene, suggesting that an increased level of dephosphorylated NPr in the ptsP mutant renders cells the filamentous growth. This idea was confirmed by the fact that cells with increased levels of dephosphorylated NPr shows the filamentation phenotype. Additionally, we showed that cell size of E. coli increases with incremental dephosphorylated NPr concentrations. These results suggested that dephosphorylated NPr induces morphological change of E. coli. 대부분의 Proteobacteria에 존재하는 질소 인산전달계는 다양한 세포내 조절에 관여하는 cascade이다. 이들은 ptsP 유전자에 의해 암호화되는 $EI^{Ntr}$, ptsO에 의해 암호화되는 NPr, ptsN에 의해 암호화되는 $EIIA^{Ntr}$로 이루어져 있다. 이들 중 $EIIA^{Ntr}$은 $K^+$ 농도 조절, ppGpp 농도 조절, 질소와 탄소 대사, ABC transporter의 조절 등 다양한 세포내 조절과정에 관여하지만, NPr의 생리적 기능에 대해서는 알려진 바가 많지 않다. 최근의 한 논문은 대장균에서 탈인산화된 NPr이 세포막 스트레스 반응에 관여한다는 사실이 밝혔다. 본 연구에서는 NPr과 관련된 새로운 표현형을 제공한다. ptsP 유전자가 결손된 균주는 filamentation 표현형을 나타내었다. ptsP 결손균주의 이런 표현형은 ptsO 유전자의 추가적인 결실에 의해 사라졌지만, ptsN 유전자의 추가적 소실에 의해서는 유지되었다. 이는 ptsP 결손균주의 filamentation 표현형이 탈인산화된 NPr의 증가 때문에 나타났음을 나타낸다. 이런 생각은 야생종에서 탈인산화된 NPr이 증가되었을 때 filamentation 표현형을 나타낸다는 사실을 통해 확증되었다. 또한 탈인산화된 NPr의 양이 증가함에 따라 대장균의 세포 길이가 점진적으로 증가한다는 사실을 알 수 있었다. 이러한 결과는 탈인산화된 NPr이 대장균의 형태적 변화를 유도함을 시사한다.

표정 인식 기반의 감성형 동반자 스마트미러

최엄지(Um Ji Choi),서준헌(Jun Heon Seo),박제훈(Je Hoon Park),박지원(Ji Won Park),박한욱(Han Wook Park),최민석(Min Seok Choi),고병철(Byoung Chul Ko) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.6

농촌 주거환경의 개선을 위한 제품디자인 제안

최엄지(Eomji Choi),조시현(Sihyun Jo),조광수(Kwangsoo Cho),홍정표(Jungpyo Hong) 한국디자인학회 2019 한국디자인학회 학술발표대회 논문집 Vol.2019 No.11

소아 폐렴의 재입원에 대한 위험인자

홍유찬,최엄지,박신애 대한소아감염학회 2017 Pediatric Infection and Vaccine Vol.24 No.3

Purpose: We analyzed the risk factors affecting readmission of children with pneumonia. Methods: We retrospectively analyzed the medical records of pediatric patients admitted to the Department of Pediatrics at the Jeonju Presbyterian Medical Center from January 2007 to August 2016. We classified patients who were readmitted with pneumonia within 30 days of discharge as the readmission group and patients who were admitted with pneumonia for the first time as the first admission group. Results: Among 158 patients, the study (readmission) group included 82 patients and the control (first admission) group included 76 patients. Age, the percentage of segmented neutrophils and lymphocytes, the number of admissions in the last 12 months, the associated diseases (respiratory diseases such as asthma), and the affection of the right upper lung were analyzed as risk factors for readmission. However, based on a regression analysis, only age and associated diseases were found to be significant risk factors. The rate of readmission increased with younger age. When there were associated diseases, the rate of readmission also increased. Conclusions: Young age and associated diseases were significant risk factors for readmission for patients with pediatric pneumonia. When pediatric patients are admitted with pneumonia, if they are young and/or have associated diseases, a comprehensive approach is needed to reduce the rate of readmission with careful consideration of precise examination, treatment, timing of discharge, and follow-up. 목적: 본 연구에서는 소아 폐렴 환자에서 재입원의 분석을 통하여 이에 영향을 미치는 위험인자를 알아보고자 하였다. 방법: 2007년 1월부터 2016년 8월까지 전주예수병원 소아청소년과에 폐렴으로 입원한 소아를 대상으로, 퇴원 후 30일 이내에 폐렴으로 재입원한 환자(재입원군)와 초회 입원한 환자(초입원군)로 나누어의무기록을 검토하여 후향적으로 분석하였다. 결과: 158명 중 연구군(재입원군)은 82명, 대조군(초입원군)은 76명이었다. 연령, 분절형 호중구 및 림프구 백분율, 12개월 내 입원 횟수, 동반 질환(천식 등 호흡기 질환), 우상 폐야의 병변이 재입원의 위험인자로 분석되었다. 그러나 회귀분석상 연령과 동반 질환만 의미 있는 차이를 보였고, 재입원율은 연령이 낮고 동반 질환이 있을 때 높았다. 결론: 소아 폐렴의 재입원 위험인자로 환자의 어린 연령과 동반 질환이 유의하였다. 소아 환자가 폐렴으로 입원했을 때 연령이 낮고 동반 질환이 있다면 더 정확한 검사와 치료, 퇴원 시기 결정, 외래 추적관찰 등에 신중을 기하여 향후 재입원율을 줄이기 위한 종합적 접근이 필요하다.

감성 인식 기반의 화장법 추천 스마트 미러

최민석(Min Seok Choi),최엄지(Um Ji Choi),서준헌(Jun Heon Seo),박제훈(Je Hoon Park),박지원(Ji Won Park),박한욱(Han Wook Park),고병철(Byoung Chul Ko) 한국정보과학회 2017 한국정보과학회 학술발표논문집 Vol.2017 No.12

Whole lung lavage with granulocyte macrophage colony-stimulating factor inhalation in children with pulmonary alveolar proteinosis: A case report

김지윤,박민지,송근백,최엄지,백은영,이다경,홍수종 대한 소아알레르기 호흡기학회 2023 Allergy Asthma & Respiratory Disease Vol.11 No.4

Pulmonary alveolar proteinosis (PAP) is a rare lung disease characterized by hypoxemic respiratory failure due to the accumulation of lipoproteinaceous material in alveolar macrophages and alveoli. It has been reported that granulocyte macrophage colony-stimulating factor (GM-CSF) inhalation or whole lung lavage significantly improves the disease state. We present a case of 17-year-old female diagnosed with PAP and treated with both GM-CSF inhalation and whole lung lavage. Her symptoms, imaging findings, and pulmonary function tests improved after these treatments. Therefore, combined treatments of whole lung lavage and subsequent GM-CSF inhalation should be considered a treatment option for children and adolescents.