Anti-Wrinkling Effect of Noni (Morinda citrifolia) by Antioxidant and Anti-Inflammatory Properties

최수철(Soo-Cheol Choi),윤영한(Young Han Youn) 인간식물환경학회 2020 인간식물환경학회지 Vol.23 No.2

Noni has been used for medicinal purposes for more than 2,000 years in South Pacific Polynesia, China and India, and has been heavily ingested as an extract for its excellent antioxidant and anti-inflammatory effects. However, a recent study found that the noni extract causes digestive disorders, kidney problems, and liver diseases, which made it necessary to use it for other purposes than as an extract. In this study, we want to evaluate the potential of noni as an anti-oxidant, anti-inflammatory and anti-wrinkling agent. Methods: Noni was freeze-dried, extracted in water, and concentrated. Skin cells were treated with the noni extract for 24 hrs and then were exposed to UVB (55 mJ/cm²). After 48 hrs of incubation, pro-inflammatory cytokine, elastase, MMP-1 and type-1 procollagen levels were measured by ELISA. Results: To find out the antioxidant effect of the noni extract, the DPPH and ABTS radical scavenging activity experiments were conducted and the noni extract showed 97.0 % and 92.0 % antioxidant efficacy at 200 μg/mL respectively. The noni extract (50 and 100 μg/mL) decreased IL-6 and TNF-α in RAW 264.7 cells induced by LPS in a concentration-dependent manner. In the RT-PCR experiment involving NO production, the noni extract (50 and 100 μg/mL) inhibited NO production by strongly inhibiting iNOS mRNA expression, and also inhibited the elevation of MMP-1 and elastases caused by UVB irradiation by 25.0 % and 7.0 % respectively. In addition, type-1 procollagen was elevated by 20.0 % by the noni extract treatment in HaCaT cells. Conclusion: The noni extract has photoprotective ability by reducing proinflammatory mediators, elastase and MMP-1 production, and elevation of collagen synthesis. Our findings suggest that the noni extract might be a good natural substance to protect against UVB-induced premature skin aging.

염증성 피부질환 개선 기능 소재로서 둥근전복(Haliotis discus) 유래 항균펩타이드 Ab4-7의 항염증 효과

최수철 ( Soo-cheol Choi ),서정길 ( Jung-kil Seo ),황준호 ( Joon-ho Hwang ),이기영 ( Ki-young Lee ),이인아 ( In-ah Lee ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

서해안에 서식하는 해양생물인 전복은 항산화 및 항염증 효과가 있으며, 항생제 개발 및 화장품 원료 등 다양한 산업에 활용될 수 있는 잠재력을 가진 자원이다. 본 연구에서 우리는 서해안의 다양한 해양 생물중에서 전복을 선택하였으며, 전복에서 분리한 항균펩타이드(AMP)를 이용하여 항균펩타이드 유도체인 Ab4-7를 규명하고 생리활성을 연구하였다. Ab4-7의 항염증 효능을 확인하기 위해 염증이 유도된 RAW 264.7에 Ab4-7을 처리한 결과, 염증성 사이토카인, TLR4, TNF-α, IL-1β, COX-2, iNOS 등을 억제하고 항산화와 관련된 유전인자인 HO-1의 mRNA 발현을 증가시켰다. Ab4-7의 강력한 항산화 및 항염증 효과를 바탕으로, 염증이 유도된 RAW 264.7 세포에서 Ab4-7이 extrocellular matrix metalloproteinase (ECM) 분해에 관여하여 다양한 염증성 피부질환을 유도하는 유전자인 matrix metalloproteinase (MMPs)를 조절하는 효과를 RT-PCR을 통해 확인하였다. Ab4-7은 강력한 항염증 및 항산화 효과를 가지고 있으며, 항염증 및 항산화 효과를 통한 MMPs 관련 유전인자 조절을 통해 다양한 염증성 피부질환 치료제의 기능성 재료로 활용될 수 있다고 사료된다. Abalone, a marine organism inhabiting the west coast of Korea, has antioxidant and anti-inflammatory effects, and is a resource with potential to be used in various industries such as antibiotic development and cosmetic raw materials. In this study, we chose abalone among various marine lives on the west coast. Antibacterial peptide (AMP) was separated from abalone and its derivative Ab4-7 was identified and its physiological activity was studied. The treatment of Ab4-7 in inflammatory RAW 264.7 to check the anti-inflammatory efficacy nhibited inflammatory cytokines, TLR4, TNF-α, IL-1β, COX-2, and iNOS and increased mRNA manifestation of HO-1, genes related to antioxidants. Based on the strong antioxidant and anti-inflammatory effects of Ab4-7, the effects of Ab4-7 in the inflammatory RAW 264.7 cells were identified through RT-PCR, which regulates the gene Matrix Metalloproteinase (MMPs) that induces a variety of inflammatory skin diseases by engaging in the decomposition of the extrocellular matrix metalloproteinase (ECM). Taken together, it is concluded that Ab4-7 has a powerful anti-inflammatory and antioxidant effect and can be used as a functional material for various inflammatory skin disease treatments by controlling the genes associated with matrix metalloproteinase (MMPs).

뷔토르 소설에서의 단절과 지속

최수철 ( Soo Cheol Choi ) 한국불어불문학회 1996 불어불문학연구 Vol.32 No.1

Les romans de Michel Butor se caracte´risent par l`activite´ d`une conscience, un effort subjectif de de´chiffrement du monde. L`attention porte´ a` un rythme essentiel de notre existence l`ame`ne a` re´aliser d`abord que notre univers mental est un monde discontinu. Il est vrai que nous ne vivons le temps comme continuite´ qu`a` certains moments. Il s`efforce donc d`e´tudier le temps dans sa continuite´. Mais la continuite´ ve´ritable est impossible dans un travail de reproduction cre´ative. C`est pourquoi il joue sur plusieurs plans de la conscience, sur le passe´ et sur le pre´sent, sur les simultane´ite´s et les retours en arrie`re. La discontinuite´ est ainsi souligne´e par la reprise renouvele´e d`une me^me matie`re dans des contextes diffe´rents. D`ailleurs, la re´alite´ e´voque´e par une perception lacunaire nous parvient sous forme de fragments. Toutefois une telle perspective re´ve`le le cheminement de Butor qui se met a` la recherche de formes mobiles pour donner une dure´e au langage, et pour de´passer la conception habituelle du temps. Dans un monde romanesque, la dichotomie entre discontinuite´ et continuite´, rupture et dure´e disparai^t par conse´quent au profit de la constitution de formes nouvelles. Butor affirme en effet que la recherche de formes romanesques nouvelles re´sulte de la tentative de confronter le langage avec la re´alite´, afin que l`un et l`autre s`e´clairent mutuellement.

ICS 극소출력 중계기를 이용한 난시청 개선

최수철(Soo-cheol Choi),조기량(Ki-ryang Cho) 한국방송·미디어공학회 2016 방송공학회논문지 Vol.21 No.1

Rhizopus japonicus와 Zymomonas mobilis의 혼합고정화 배양계에 의한 생전분으로부터 에탄올 생산

최수철(Soo-Cheol Choi),이상원(Sang-Won Lee),박석규(Seok-Kyu Park),성찬기(Chan-Ki Sung),손봉수(Bong-Soo Shan),성낙계(Nack-Kie Sung) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.4

호기성의 Rhizopus japonicus와 협기성 Zymomonas mobilis로 구성된 혼합고정화 배양계(R-Z계)를 제조하고, 생전분으로부터 에탄을 생산에 응용하였다. R. japonicus를 고정화배양하므로서 액체배양에서 보다 2배 높은 glucose량을 얻었다. R-Z계의 에탄을 생산량은 1.67g/L(Y_(P/S), 0.094)이었지만, 배양 24시간째부터 산소공급을 억제한 R-Z 24계에서는 6.54g/L(Y_(P/S), 0.38)의 에탄올을 얻어 대조구의 약 4배를 향상시켰다. 회분배양에서는 5%의 기질 농도가 적당하였으며, 생산된 에탄올은 15.02g/L(Y_(P/S), 0.36)이었다. 2% 기질을 5회 첨가한 유가배양에서는 2%기질의 회분배양과 동등한 수율인 0.38을 얻었다. Ethanol production from raw starch was performed using the co-immobilized culture system of Rhizopus japonicus and Zymomonas mobilis(R-Z). Glucose production in immobilized R. japonicus culture was 2-fold higher than that in free cell culture. Ethanol production was 1.67g/L(Y_(P/S), 0.094) and 6.54g/L(Y_(P/S). 0.38) in R-Z and R-Z 24 culture system, respectively. R-Z system was modified and designated as R-Z 24 system by replacing cotton plug with silicon check valve after 24h fermentation with R-Z system. Optimal substrate concentration for ethanol production in batch culture was 5%(w/v) and ethanol concentration produced was 15.02g/L(Y_(P/S), 0.36). Ethanol yield(Y_(P/S), 0.38) in fed-batch culture of 5 times with 2%(w/v) substrate was equal to that in batch culture of 2% (w/v) substrate.

DSS로 유도된 마우스 대장염 모델에서 꽃새우 추출물의 항염증 효능

최유민(Yu Min Choi),박지선(Ji Sun Park),강정은(Jeong Eun Kang),최수철(Soo Cheol Choi),이성훈(Sung Hoon Lee),이인아(In-Ah Lee) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.9

꽃새우는 우리나라 서 · 남해안에 분포하며 철분, 칼슘, 타우린 등 다양한 영양소가 들어있다. 본 연구에서는 꽃새우 추출물을 이용하여 LPS로 염증이 유도된 RAW 264.7 세포와 DSS로 염증이 유도된 ICR mouse 모델에서 항염증 효과를 실험하였다. RAW 264.7 세포와 마우스의 대장 조직을 이용해 RT-PCR을 진행하였을 때 꽃새우 추출물 투여는 염증성 사이토카인인 IL-6, TNF-α, IL-1β와 염증매개 물질인 iNOS, COX-2의 발현이 감소하였다. 또한, 대장 조직 내 단백질 발현을 유의하게 감소시켰으며, 대장 조직 손상을 개선하였다. 이와 같은 연구 결과를 보아 꽃새우 추출물은 in vivo와 in vitro에서 염증성 사이토카인을 억제하는 효과가 있음을 제시하고 있다. 꽃새우가 염증성 장 질환 치료 및 예방에 효과적인 물질로서의 가치를 입증하기 위해서는 염증 반응에 영향을 미치는 성분 확인이 필요하다고 사료된다. Trachysalambria curvirostris is a crustacean found near the southwest coast of Korea which contains various nutrients such as iron, calcium, taurine, and chitosan. In particular, chitosan has anti-inflammatory, antibacterial, anticancer, and antioxidant effects. The purpose of this study was to evaluate the anti-inflammatory effect of the extract of Trachysalambria curvirostris on inflammatory bowel disease (IBD). Inflammatory factors (COX-2, IL-1β, IL-6, iNOS, TNF-α) in cells and colon tissue induced by lipopolysaccharide (LPS) and dextran sulfate sodium (DSS) were confirmed using RT-PCR. The generation of cytokines (IL-6, TNF-α) in the colon tissue protein of ICR mice caused by DSS was measured by ELISA. Compared to the LPS and DSS groups, the expression level of inflammatory cytokines showed a reduction in the group treated with the Trachysalambria curvirostris extract. Similarly, the ELISA results showed that the expression level of inflammatory cytokine decreased compared to the DSS group. As a result of H&E staining using mouse colon tissue, it was possible to observe a lower invasion of inflammatory cells in the Trachysalambria curvirostris treated group than in the DSS group. It is necessary to conduct additional research on the exact mechanism of reaction to identify the active components in the Trachysalambria curvirostris extract, and to prove the value of the Trachysalambria curvirostris for IBD prevention and treatment.

항균 펩타이드 Ab4-3의 항염증 및 항산화 활성 스크리닝

송준(Song, Jun),최수철(Choi, Soo-Cheol),이인아(Lee, In-Ah) 한국생활과학회 2023 한국생활과학회 학술대회 Vol.2023 No.5

참마와 명아주의 항산화 및 항염증 효과

김신애 ( Shin Ae Kim ),최수철 ( Soo Cheol Choi ),윤영한 ( Young Han Youn ),고창인 ( Chang In Ko ),하영순 ( Young Soon Ha ),이인아 ( In-ah Lee ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.4

본 연구에서는 참마와 명아주의 항산화 및 항염증 효능을 평가하기 위해 참마와 명아주 에탄올 추출물을 이용하여 free radical 소거활성, enzyme-linked immunosorbent assay (ELISA) 실험을 수행하였다. 참마와 명아주 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(EC₅₀)에서 각각 2.386, 0.524 mg/mL로 측정되었다. 또한 참마와 명아주 추출물의 혼합 시료의 free radical 소거활성은 참마 추출물 : 명아주 추출물 비율이 2 : 1일 때 가장 뛰어난 것으로 나타났다. IL-6와 TNF-α의 ELISA 실험을 통해 항염증 효능을 평가한 결과, 마우스 비장세포에서 IL-6의 경우 1 mg/mL 농도에서 참마 추출물은 대조군과 대비하여 27.17% IL-6 생성을 감소시켰으며, 명아주 추출물은 72.30%의 감소를 나타내었다. TNF-α의 경우 참마 추출물은 61.97%, 명아주 추출물은 77.85%로 유의성 있는 TNF-α생성 감소 효능을 나타내었다. 이 결과들을 통하여 참마와 명아주 추출물은 항산화, 항염증 효능을 가지고 있으며, 이를 활용하여 항염증에 효과가 있는 천연물 제제에 응용 가능성이 있음을 확인하였다. In this study, free radical scavenging activity and enzyme-linked immunosorbent assay (ELISA) experiments were carried out using Dioscorea japonica (D. japonica) and Chenopodium album (C. album) extracts to evaluate their anti-oxidative and anti-inflammatory effects. In the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity, EC₅₀ of D. japonica and C. album were measured as 2.386 and 0.524 mg/mL, respectively. The free radical scavenging activity of the mixed sample of D. japonica and C. album was the highest when the D. japonica and C. album ratio was 2 : 1. The IL-6 and TNF-α ELISA assay showed that IL-6 in mouse spleen cells treated 1 mg/mL of samples, D. japonica and C. album decreased the production of IL-6 concentration by 27.17%, 72.30%. In the case of TNF-α, D. japonica and C. album decreased 61.97% and 77.85% of TNF-α production, respectively. Through these results, we confirmed that D. japonica and C. album have antioxidant and anti - inflammatory effects and could be applied to natural medicine cosmetic having anti - inflammatory effects.