Expansin 유전자를 이용한 담배의 형질전환

최동수,김호방,김정회,신주식,석영선,정찬문,이이,Choi Dongsu,Kim Ho-Bang,Kim Jeong-Hoe,Shin Ju-Sik,Seok Yeong-Seon,Chung Chan-Moon,Lee Yi 한국연초학회 2005 한국연초학회지 Vol.27 No.2

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.

전투기 날개 수리를 위한 기계적 체결의 형상 선정에 관한 연구

최동수,Choi, Dongsu 한국군사과학기술학회 2021 한국군사과학기술학회지 Vol.24 No.5

A study on optimal shape selection of a mechanical fastening for the repair of crack defect of ROK Air Force F-5 fighter wing was conducted. The crack defect occurred in the spar of the wing, and the technical manual does not specify the repair method. However, ROK Air Force decided to develop a repair technology for this defect in consideration of various logistic conditions. Three repair shapes for the proper repair were devised and the finite element analysis was performed to examine the structural safety of these three connection members. As a result of the structural safety review, two connection members except one were structurally safe with safety margins over zero because the calculated stress values were at or below the yield strength level. Therefore, two connection members were determined to be able to use for repair under the condition that the aircraft operated within the design limit load. The results of this study would be very useful if the same defect occurs in long-term aircraft operated by the ROK Air Force.

반월공단지역의 잣나무 및 리기다소나무의 생장과 광합성 특성

이충화(Choong Hwa Lee),최동수(Dongsu Choi),이승우(Seung-Woo Lee),김은영(Eun-Young Kim),진현오(Hyun-O Jin),정진현(Jin-Heon Jeong),이천용(Chun-Yong Lee),오정수(Jeong-Soo Oh) 한국산림바이오에너지학회 2004 산림바이오에너지 Vol.23 No.1

본 연구의 목적은 현재 산림쇠퇴 징후가 나타나고 있는 반월공단지역에 식재되어 있는 잣나무와 리기다소나무의 생장저하 실태와 광합성 특성을 밝히는데 있다. 이를 위해 안산시 전망대공원에 피해구를, 경기도 광주시에 소재하고 있는 경희대학교 연습림에 대조구를 선정하여 인공식재된 약 10년생의 잣나무와 리기다소나무 2수종을 비교하였다.<br/> 잣나무 및 리기다소나무 2수종 모두 피해구에서 생장량이 감소하였으며, 침엽의 잔존율도 저하하였다. 또한 2수종 모두 피해구에서 대조구에 비하여 순광합성속도가 저하하였고, 광-광합성곡선의 초기기울기인 양자수율도 저하하였다. 클로로필 a+b, b의 함량도 피해구에서 감소하였으며, 이 결과는 침엽의 연령이 증가함에 따라서 집광운반능력이 낮아져 침엽의 잔존율이 낮아지는 결과를 초래하였다고 생각된다. 이상의 결과로부터 최근 대기오염이 심한 반월공단지역에서 생육하고 있는 잣나무 및 리기다소나무의 생장감소 원인은 침엽의 클로로필 함량이 광화학계의 활성을 저하시켜 광합성 능력이 감소되어 최종적으로 생장감소를 초래한 것으로 판단된다. This study was performed to investigate the growth and photosynthetic rate of ten-years old Pinus koraiensis and Pinus rigida in Banwol industrial region comparing with those of the control site in Gwangju, Gyeonggi Province. Surface soil of the damaged in pH 4.2 was more acidic than control site in pH 4.8, assuming the accelerated soil deterioration by acid deposition.<br/> The shoot growth, needle survival rate and net photosynthetic rate of P. koraiensis and P. rigida at the damaged site were lower than at the control site. In addition, the quantum yield and chlorophyll a+b and b contents reduced in damaged treatment. These results suggest that an inhibition of photochemical reaction of P. koraiensis and P. rigida at the damaged site of Banwol industrial region could be attributed to complexly influence of atmospheric pollution and soil acidification.

항공조명을 위한 NVIS 친밀 발광다이오드 개발 (II) : 녹색

김태훈(Tae Hoon Kim),최동수(Dongsu Choi),유창한(Chang Han Yu) 항공우주시스템공학회 2023 항공우주시스템공학회 학술대회 발표집 Vol.2023 No.10

항공조명을 위한 NVIS 친밀 발광다이오드 개발 (I) : 백색

김태훈(Tae Hoon Kim),박광일(Kwang Il Park),최동수(Dongsu Choi),김외철(Oe Cheul Kim),유창한(Chang Han Yu) 항공우주시스템공학회 2023 항공우주시스템공학회 학술대회 발표집 Vol.2023 No.10

항공기 주기환경이 대기부식위험도에 미치는 영향

윤주희 ( Juhee Yun ),이두열 ( Dooyoul Lee ),박승렬 ( Sungryul Park ),김민생 ( Min-saeng Kim ),최동수 ( Dongsu Choi ) 한국부식방식학회(구 한국부식학회) 2021 Corrosion Science and Technology Vol.20 No.2

Atmospheric corrosion severity associated with aircraft parking environment was studied using metallic specimens, and temperature and humidity sensors installed at each aircraft operating base. Data were analyzed after a year of exposure. Silver was used to measure chloride deposition by integrating X-ray photoelectron spectroscopy depth profiles. Carbon steel was utilized to determine the corrosion rate by measuring the weight loss. The time of wetness was determined using temperature and humidity sensor data. Analysis of variance followed by Tukey’s “honestly significant difference” test indicated that atmospheric environment inside the shelter varied significantly from that of unsheltered parking environment. The corrosion rate of unsheltered area also varies with the roof. Hierarchical clustering analysis of the measured data was used to classify air bases into groups with similar atmospheric corrosion. Bases where aircraft park at a shelter can be grouped together regardless of geographical location. Unsheltered bases located inland can also be grouped together with sheltered bases as long as the aircraft are parked under the roof. Environmental severity index was estimated using collected data and validated using the measured corrosion rate.

Isolation and Characterization of Expansin Genes in a Halophyte, Suaeda japonica

Soong-Taek Hwang(황숭택),Suk Kyu Kim(김석규),Jong Gil Na(나종길),Jeom Sook Lee(이점숙),Dongsu Choi(최동수) 한국생명과학회 2013 생명과학회지 Vol.23 No.2

본 연구에서는 염생식물에서는 최초로 expansin 유전자를 분리하고 특성을 분석하였다. 염생식물 중 생리활성 물질 등으로 최근 들어 관심의 대상이 되고 있는 칠면초로부터 expansin 유전자의 cDNA 3종을 분리, 합성하였다. 확보한 3종의 칠면초 expansin cDNA 염기서열 분석 및 단백질 1차 구조의 비교분석 결과 시스테인 잔기의 위치 및 배열간격과 트립토판의 위치, 그리고 활성부위의 일부로 생각되는 HFD 도메인이 잘 보존되어 있다는 점에서 이들이 α- expansin (EXPA)에 속한다는 것을 확인하였다. 다른 식물들과 칠면초의 expansin 단백질을 대상으로 계통수를 작성한 결과, 목본성인 딸기와 대추나무의 expansin과 가장 가까운 것으로 나타났다. 칠면초는 일년생 초본이지만 생육초기에는 초본성으로 자라다가 나중에 목질화되는 경향이 있다. 따라서 칠면초의 expansin이 목본 식물의 expansin과 유사한 기능을 가질 것으로 추측할 수 있다. 고염도 환경에서의 칠면초 유식물의 생육 실험결과에 따르면 실험에 사용된 NaCl 농도범위 내에서 유식물의 생장이 현저하게 억제되지 않았을 뿐만 아니라 expansin의 발현량에도 큰 변화가 없었다. 이 결과로 미루어 내염성을 가진 식물에서는 expansin과 같은 생장관련 유전자의 발현이 NaCl의 농도에 크게 영향을 받지 않으므로 식물의 생장이 저해되지 않는다고 유추할 수 있다. 이는 염생식물인 칠면초가 고염도 환경에서 정상적으로 생육할 수 있도록 하는데 expansin이 중요한 요소 중 하나일 것이라는 점을 의미한다. Halophytes are unique land plants that are capable of thriving in a high?salt environment. They are attracting public attention due to their ability to synthesize bioactive substances such as UV protectants or antioxidizing agents. To achieve unaffected growth under high salinity, halophytes may take advantage of the activities of cell growth factors such as expansins. Expansins are well-known cell wall proteins that are responsible for cell enlargement. They loosen cell walls, thereby contributing to actual plant growth. This study aimed to identify positive roles of expansins in the growth of halophytes. Three expansin cDNA clones were isolated from seedlings of Suaeda japonica. Comparing the deduced amino acid sequences of the expansin genes of S. japonica with those of other plant species suggested that the cDNA clones isolated from S. japonica belong to the EXPA (α-expansin) gene family. A phylogenetic tree based on the deduced amino acid sequences revealed that the expansins of S. japonica share a close evolutionary relationship with those of strawberry (Fragaria ananassa) and jujube (Ziziphus jujuba), both of which are woody dicots. SjEXPAs did not show any remarkable change in the gene expression level in different NaCl concentrations, providing a clue to the unaffected seedling growth of S. japonica in a high-salt environment. In conclusion, the present study presents the first report of expansin genes from halophytes and suggests a putative role for these genes in plant growth under high salinity.