Propolis 추출물의 병원성 미생물에 대한 항균 효과

조정순,김영후,권명상,Cho Jung-Soon,Kim Young-Hwu,Kwon Myong-Sang 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.4

The optimal concentration of ethanol to separate a high quantity of propolis was $60\%$ but that for the best flavonoids extraction was $80\%$ We compared the yields of propolis from different countries. In this study we used $60\%$ ethanol concentration as a standard. The yield of propolis was proportional to the contents of flavonoids. Namely, Polish propolis which showed the highest yield with $56\%$ by the extraction with $60\%$ ethanol revealed also the highest flavonoids content with $3.49\%$ among all the samples tested The major constituents of propolis differed from country to country. It has been suggested that the different geographical origin influenced the efficacy and the constituents of propolis. Antibacterial activity of ethanol extracted propolis from different countries was tested against 6 microbial strains of type cultures including Gram-positive (Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae) and Gram- negative bacteria (Klebsiella pneumoniae, Proteus vulgaris and E coli) in vitro. Propolis extract showed anti-microbial activity against all the tested bacterial strains. In addition, propolis was sensitive to E coli which was resistant to broad spectrum antibiotics like ampicillin. These results showed that propolis may substituted for commercial antibiotics. The efficiency of anti-microbial activity of the propolis was slightly higher in $80\%$ than $97\%$ ethanol extract.

유아를 위한 창의성 프로그램 개발

趙廷順(Cho, Youn-Soon),趙夕嬉(Cho, Seok-Hee),洪勇姬(Hong, Young-Hee),金貞孝(Kim, Jung-Hyo) 이화여자대학교 교육과학연구소 1996 교육과학연구 Vol.24 No.-

한국 골프전문인력의 일자리 정책 발전방안

조정순(Jung-Soon Cho),서아람(Ah-Ram Suh) 한국엔터테인먼트산업학회 2020 한국엔터테인먼트산업학회논문지 Vol.14 No.4

본 연구는 골프분야의 전문인력 현주소를 파악하여 골프산업에서의 일자리를 확대할 수 있는 방안을 강구하기 위한 기초자료를 제시하는데 목적을 두었다. 이를 위한 연구의 주요내용은 한국의 골프직업 및 전문인력 현주소, 한국 골프전문인력의 직업적 발전 방향을 다각적으로 분석하여 제시하였고 전문적, 직업적인 골프 일자리 창출을 위한 정책들의 발전방안들을 다음과 같이 제안하였다. 첫째, 대학과 사회가 연계될 수 있는 실용적인 교육과정 개편 확대를 제안하였다. 이를 위해 골프교육 분야에서도 국가직무능력표준(NCS)의 적극적인 도입 및 활용뿐만 아니라 골프현장에서 적용할 수 있는 마케팅 능력을 겸비한 다양한 골프전문가 등을 양성해야 할 것이다. 둘째, 골프클럽디비전 프로그램의 적극적인 도입과 추진을 제안하였다. 문화체육관광부와 대한체육회에서 정부지원 사업으로 적극적으로 추진하고 있는 상황이며, 골프클럽디비전 사업 추진을 통해 우수인재 확보, 은퇴선수 효율적 관리, 다양한 연령층의 생활체육 골프 참여 문화 조성 등 운영 체질이 개선될 것이다. 셋째, 골프 취업자 전수조사를 제안하였다. 골프분야 취업자 현황도 체육지도자 범주 속에서 별도의 현황 파악이 필요하며, 이는 고용안정과 함께 제도적인 접근을 모색하기 위해 반드시 골프종사자에 대한 취업 전수조사가 체계적으로 이루어져야 할 것으로 판단된다. 넷째, 전문적이고 특화된 골프직업군의 개발과 전개를 제안하였다. 국가 발전의 동력을 위해 골프전공자들의 사회 진출 선택권이 넓어지고 학생선두들이 은퇴 후에도 안정적인 직장을 갖고 골프인들이 자긍심을 가질 수 있도록 해야만 한다. 특히, 생활체육분야에서의 골프복지사, 장애인 및 노인 등 대상을 위한 골프여가치료사, 그리고 해양스포츠와 골프관광의 융합상품의 개발도 새로운 일자리 창출 영역으로 판단된다. 다섯째, 골프산업 경쟁력의 강화를 제안하였다. 이를 위한 기업 차원에서는 가격의 거품을 빼고 낮추며, 품질 및 기술력에 대한 적극적인 투자, 아울러 홍보 부분 강화가 필요하다고 본다. 또한 정부 차원에서는 다양한 정부지원의 제공과 분위기 조성, 전문 인력의 양성과 기술개발 투자와 함께 적극적인 홍보가 필요할 것으로 판단된다. The purpose of this study is to provide adequate datas on number of people who are in golf related field and also further expend that number for golf industry. The main stream of this study is to map out present golf related jobs and how this study can help golf industry in general. To make a greater improvements on golf industry with more job opportunity following ideas were presented. First, improving education on “hand on experiences on the field of golf industry” Better educating potential employees for golf industry for the right positions can enhance overall work environment. To do so, the society and the schools must come to agreement to provide adequate curriculum for people. Second, implementing “a golf club division program.” The support from Ministry of Culture and Sports and Tourism Department, which are govern by Republic of Korea, are aggressively working to expend the golf business and also recruit elite personnel like former tour players to work on the field to better operate the whole system. Third, performing a thorough research on current golf related jobs and numbers and diversity on the field. Fourth, developing a specific and a differentiated golf job fields for better future for people and students who majored in golf industry. So that they cam feel secured and have a sense of dignity. Finally, strengthening the golf industry’s competitiveness. The golf related companies have to step up to higher gear. With working in harmony between golf industry and government can assure of brighter future for our next generation.

유지의 영양과 질병

조정순 ( Jung soon Cho ) 한국유화학회 1997 한국응용과학기술학회지 Vol.14 No.3

수원 5호 메밀 채소 추출물의 유리라디칼 소거 및 Xanthine Oxidase 활성 저해

김영순,조정순,서형주,Kim, Young-Soon,Cho, Jung-Soon,Suh, Hyung-Joo 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.2

메밀 채소 아세톤 추출물을 각 용매로 분획시 회수율은 헥산이 57%로 가장 높은 회수율을 보였으며, 페놀 성분의 양은 아세트산에틸과 1-부탄올 분획이 47.96 mg/ml과 44.02 mg/ml로 높은 함량을 보였다. 수소 공여능은 부탄오 분획이 가장 높았으며, 에테르, 헥산 순으로 나타났다. 과산화지질의 형성 억제능은 기질로 리놀레산을 사용하여 자외선 조사에 대한 산화 억제능으로서 검토하였다. 과산화지질의 형성 억제능은 부탄올과 헥산 분획에서는 0.9% 첨가시 1시간 조사한 결과 65%와 57%의 억제능과 2시간 조사시 75%와 60% 억제능을 보였다. 초과산화물음이온 라디칼 소거 활성의 측정은 xanthine/xanthie oxidase를 이용한 superoxide dismutase 활성 측정법을 이용하였다. 각 분획의 초과산화물음이온 라디칼 소거 활성은 부탄을 분획은 392.6 U/mg으로 가장 높은 SOD의 활성, $IC_{50}$값은 $2.5\;{\mu}g$으로 가장 낮은 값을 보임에 따라 다른 분획에 비해 초과산화물음이온 라디칼 소거능력 이 가장 높았다. 또한 각 분획의 spectrophotogram을 검토한 결과 부탄올 분획은 메밀의 활성 성분인 rutin과 유사한 spectophotogram을 보였다. 초과산화물음이온 라디칼 소거능과 rutin 함량이 밀접한 관계가 있음을 확인하였으며, 각 분획의 xanthine oxidase의 활성 저해는 $IC_{50}$값이 $3.1\;{\mu}g$을 보인 부탄올 분획의 저해효과가 가장 높았다. To examine the characteristics of the antioxidative property of Buckwheat components, acetone extracts from a buckwheat, Suwon 5, was fractionated using five solvents. Hexane, ethylacetate, ether, butanol and water fractions were obtained. Butanol fraction showed the greatest electron donating ability and inhibitory effect on lipid peroxidation. It also showed the most excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. Spectrophotogram of butanol fraction was similar to that of rutin. Superoxide radical scavenging activity was related to the contents of rutin. Inhibitory effect of each fraction on xanthine oxidase was also measured. Butanol fraction had the strongest inhibitory effect on xanthine oxidase and $IC_{50}\;was\;3.1\;{\mu}g$. The inhibition type of butanol fraction on xanthine oxidase turned out to be a mixture of the uncompetitive and non-competitive modes.

육가공 제품의 저장 및 조리방법이 지질산패에 미치는 영향

이재민,조정순 ( Jae Min Lee,Jung Soon Cho ) 한국유화학회 1995 한국응용과학기술학회지 Vol.12 No.1

Ham, sausage and bacon were treated with common household processing techniques including refrigerated storage(0, 14, 28 days) and cooking(pan-frying, microwaving, boiling). Lipid oxidation was evaluated by measuring fatty acid composition, malonaldehyde(MA), TBA values and by measuring fluorescent products. Major fatty acid composition were oleic acid and followed respectively palmitic acid, stearic acid, linoleic acid, linolinic acid. There was no significant difference in fatty acid composition by cooking method but there was a tendency of being increased of unsaturated fatty acid during 28days storage. Ma, TBA and fluorescent products showd a tendency of being increased continually according to storage days rather than cooking method.

천연 생약 복합 추출물이 Streutozotocin 유발 당뇨성 흰쥐의 혈당 및 인슐린, 혈청 과산화지질 농도에 미치는 효과

박형래,조정순,Park, Hyung-Rea,Cho, Jung-Soon 동아시아식생활학회 2007 동아시아식생활학회지 Vol.17 No.2

This study was designed to investigate a natural medicinal multi-plants extract (BG515), which consisted of multi extracts of Mori folium, Rehmannia glutinosa Liboschitz, Dioscorea japonica, Lycii fructus, and Astragalus radix, on blood glucose, insulin levels, and serum malondialdehyde (MDA) concentrations in streptozotocin-induced diabetic rats. Streptozotocin (STZ) induces a type 1 diabetes mellitus in rats. Type 1 is usually characterized by the presence of islet cell autoantibodies (ICA), autoantibodies to insulin (IAA), and autoantiboides to glutamic acid decarboxylase (GAD), which identify the autoimmune process that leads to $\beta-cell$ destruction. Thirty-five male Sprague-Dawley (SD) rats weighing $150\sim170g$ each (6 weeks old) were randomly divided into one control (Group A) and 4 STZ-induced diabetic groups, and were subjected to one of the following treatment for 12 weeks. Groups A and B were fed basal diets and Group C, D, and E received the same diets as groups A and B, but with supplements of 150 mg/kg, 300 mg/kg, and 600 mg/kg of BG515 orally for 12 weeks, respectively. Diabetes was induced in Groups B, C, D, and E by intravenous injection of 45 mg/kg of STZ per body weight in sodium citrate buffer (pH 4.5) via the tail vein. In the BG515 groups, we found increases in serum insulin levels, compared to the STZ-control group, but these data were not significant. In contrast, blood glucose and serum MDA concentrations decreased in the BG515 groups compare to the STZ-control group. At the 5th week, in all the BG515 administered groups, there were decreases in serum blood glucose levels compared to the STZ- control group, and this activity was very strong in the BG515-1 group at the 12th week. These results suggest that natural bio-complex compounds (BG515) may slightly suppress STZ-induced changes in serum MDA concentration via the maintenance of serum insulin levels, due to the prevention of $\beta-cell$ and glucagon destruction by STZ.

간함량비율과 제조방법에 따른 간소시지의 지질 산패에 관한 연구

이숙미,오성천,조정순 ( Sook Mi Lee,Sungcheun Oh,Jung Soon Cho ) 한국유화학회 1997 한국응용과학기술학회지 Vol.14 No.1

Objectives of this study were to investigate the lipid oxidation of liver sausages, one of the most favorite meet products and to examine the applicability of pork liver to sausages. Sausages containing different proportions of pork liver were manufactured with various processes and were tested and analysed by means of lipid oxidation. Based on the basic recipe for manufacturing sausages, fifteen recipies were proposed with different combinations of ingredients, followed by evaluations on their contents of crude fat, AV, POV, and MA. Sausages manufactured with different methods(sliceable, spreadable, smoked spreadable) and different proportions of liver were also evaluated. In addition, the storage experiments were carried out. The findings from these experiments are summarized as follows : The content of crude fat was 17.12%. As the percentage of the liver increased, fat content of sausages siginificantly decreased. In the storage experiment, it was found that the acid value. peroxide value, and malonaldehyde content significantly increased in all samples regardless of the amount of liver or the storage time. The AV of experimental sausages was significantly increased by adding liver. There were significant differences in proportions of components but little differences were observed in contents among the samples.

Propolis가 종양 억제작용에 미치는 영향

권명상,김영후,조정순,Kwon Myung-Sang,Kim Young-Hwu,Cho Jung-Soon 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.5

In recent years, propolis has attracted much attention as an useful substance in medicine and functional food, even if it is known as a natural remedy in folk medicine since ancient times. propolis was registered as natural food since 1995 on Korean Food Act by Korean Food and Drug Administration(KFDA). The present study demonstrated the optimization of isolation of crude propolis by ethanol, and tumoricidal effect of pro polis. The optimal concentration of ethanol to separate a high quantity of propolis was $60\%$. The cytotoxic effect of ethanol extracted propolis against various cancer cell lines including murine lymphoma (Sarcoma-180), murine T-lymphoma (YAC-1), human breast carcinoma (MCF-7), human gastric carcinoma (KATOIII) and human hepatocellular carcinoma (Hep3B) and human lung adenocarcinoma (A-549) was observed using SRB and MIT assay. In order to investigate the curative activity by oral administration of propolis on tumor, ICR mice was subcutaneously implanted Sarcoma 180. In 300mg/kg and 600mg/kg propolis administered group, development of implanted tumors was inhibited by $40.9\%\;and\;67.9\%$ at 16th day, respectively. In the same dose of propolis administered group, development of implanted tumors was inhibited more strongly with dose dependent manner. Therefore, these data suggested propolis may show tumoricidal effects. In conclusion, these results indicate that propolis, one of the few natural remedies, can be used as functional food with tumoricidal effects.

수원 5호 채소 추출물의 유리라디칼 소거 및 Xanthine Oxidase 활성 저해

서형주,김영순,조정순 ( Hyung Joo Suh,Young Soon Kim,Jung Soon Cho ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.3

To examine the characteristics of the antioxidative property of Buckwheat components, acetone extracts from a buckwheat, Suwon 5, was fractionated using five solvents. Hexane, ethylacetate, ether, butanol and water fractions were obtained. Butanol fraction showed the greatest electron donating ability and inhibitory effect on lipid peroxidation. It also showed the most excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. Spectrophotogram of butanol fraction was similar to that of rutin. Superoxide radical scavenging activity was related to the contents of rutin. Inhibitory effect of each fraction on xanthine oxidase was also measured. Butanol fraction had the strongest inhibitory effect on xanthine oxidase and IC_(50), was 3.1 ㎍. The inhibition type of butanol fraction on xanthine oxidase turned out to be a mixture of the uncompetitive and non-competitive modes.