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농약의 약효증진을 위한 첨가제 효과에 관하여 - Diazinon 제를 중심으로 -
조정례,이규승 ( Jeong Rye Cho,Kyu Seung Lee ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.1
In this paper, we reviewed the degradation factors of diazinon which was known to be easily degraded by soil microorganisms and lost of its activity. Under submerged soil condition, the contribution of microorganisms to diazinon degradation was about 40% and these microorganisms preferred soil humus as substrates to diazinon itself. The effect of monooxygenase activity in submerged soil was more important than esterase activity on diazinon degradation and these enzymes were inhibited by several chemicals such as piperonyl butoxide(PBO), EPN and tricyclazole. From these results, new formulation type of diazinon (PBO and triphenyl phosphate were added to commercial diazinon formulation by 0.1% respectively.) and diazinon mixture formulation (diazinon was mixed with EPN, tricyclazole and carbofuran in equal amount) were prepared. The new formulation type of diazinon showed better insecticidal activity by 12% and more delayed diazinon degradation in ten days than commercial diazinon.
崔鍾佑,趙丁禮,李奎承,金文圭 충남대학교 농업과학연구소 1992 농업과학연구 Vol.19 No.2
The effects of continuous restoration of sludge ito the reclamating paddy soil and teaching test of sludge components by soil column were investigated. 1. The contents of nitrogen, phosphorus, potassium, C.E.C. and organic matter(O.M.) were increased in/on the paddy soil treated with paper mill sludge than non-treated. 2. Humic layer depth recognized by color showed the non-treated(10 cm), second year(15 cm) and third year(20 cm), respectively. 3. The effects of sludge treatment showed in the contents of O.M. such as non-treatment(0.9 %) < second year(1.39 %) < third year(1,75 %) in 10 cm depth. 4. All components in soil tested with column were increased by holding capacity of sludge, and the contamination effects of soil and ground water were not found by leaching test.
李奎承,趙丁禮,崔鍾佑 忠南大學校 環境問題硏究所 1992 環境硏究 Vol.10 No.-
금강상류 용담댐 예정지구내의 금강본류와 지천을 대상으로 하여 7 개소를 선정하여 1992년 5월 30일과 11월 29-30 일의 2회에 걸쳐 현장에서 채취하여 수질을 조사하였으며, 중요한 결과는 다음과 같다. 1. pH는 대체로 수질기준에 적합하였으나, 1차 채수시 금강본류 3개지점에서 이끼 그리고 진안천 하류에는 수초가 왕성하게 자라고 있음을 확인하였다. 2. BOD의 경우 1차시료에서는 금강본류가 유입천 보다 낮았고, 2차시료에서는 본류의 BOd가 상승하는 경향을 보였으며, 지안천하류(L-6)가 1, 2차 시료 모두 가장 높은 함량을 나타냈다. 3. 생활하수와 관련있는 Na+와 Cl-의 함량은 진안천 하류(L-6)에서 가장 높았으며, NH4-N와 NO3-N도 같은 경향이었으므로 진안읍이 금강상류에서 오염원으로 문제가 되고 있음을 알수 있었다. 4. 용담댐 예정지역의 수질은 비교적 양호하였으나, 진안읍의 생활하수와 유역 농경지 토양에서 유입되는 비료성분이 주오염원으로 생각되며, 특히 금강최상류의 죽도유원지는 하절기 수질오염으로 크게 문제시 될 것으로 본다.
잿빛곰팡이병균(Botrytis cinerea) 의 Procymidone 약제 저항성 요인 구명
이규승,조정례 한국환경농학회 1995 한국환경농학회지 Vol.14 No.3
잿빛곰팡이병균(Botrytis cinerea)의 procymidone 약제 저항성 발현 요인을 구명하기 위하여 procymidone 약제 저항성과 감수성 균주를 각각 세균주씩 선발하여 약제 저항성 검정과 아울러 세포구성물질의 함량 및 조성을 조사한 결과를 요약하면 아래와 같다. 1. 공시균주의 약제 저항성 검정 결과 저항성이 감수성균주에 비하여 1,000배 이상의 저항성이 발현된 것으로 확인되었다. 2. Monooxygenase저해제인 piperonyl butoxide와 esterase 저해제인 triphenyl phosphate를 PDA 배지에 처리한 후 균사생육을 조사한 결과 저항성과 감수성균주간에 차이가 없어 저항성은 균체의 내적요인에서 기인된 것으로 판단되었으며, 아울러 procymidone과 혼합 처리시 약효 상승 효과는 확인되지 않았다. 3. 스테롤 조성은 저항성과 감수성 균주 모두 유사하였으며, GC/MS 분석결과 분자량 428의 스테로이드계 화합물이 비슷한 함량으로 존재하였다. 4. 지질함량은 저항성이 감수성균주에 비하여 30% 정도 많았고, 지방산 함량은 저항성균주의 경우 palmitic acid와 stearic acid가 약 2.5배, oleic acid 2.9배, 그리고 linoleic acid가 공시균주의 주된 지방산으로 나타났으며, 2배 정도 많은 함량을 보였다. 5. 조키틴 함량은 감수성균주가 저항성에 비하여 0.11% 많았으나, 이의 가수분해산물인 N-acetyl glucosamine함량은 저항성균주가 감수성에 비하여 반응 24시간에 3배 그리고 48시간에서 2배 많았다. 따라서 잿빛곰팡이병균(Botrytis cinerea)의 procymidone 약제 저항성은 lipid 함량의 증가, fatty acid 함량의 증가 및 조성의 변화 그리고 키틴 전구물질인 GlcNAc 함량과의 복합적인 요인에 의하여 발현되었을 것으로 판단되었다. This study was carried out to investigate the resistance mechanism by three different kinds of procymidone-resistant and susceptible isolates of Botrytis cinerea which had been collected from green houses. The average resistance level of the resistant strains was 1,000 times higher than that of susceptible ones. Also, it was revealed that the resistance was not originated from components excreted by Botrytis cinerea, based on the result obtained from the treatment with piperonyl butoxide and triphenyl phosphate as an inhibitor of monooxygenase and esterase, respectively. The total lipod content of resistant strains was 1.3 times higher than that of susceptible ones, among fatty acids, palmitic acid, stearic acid, and linoleic and being 3.0, 2.5, and 2.0 times higher, respectinely. Also slight differences in sterol contents and components were observed. The crude chitin content was slightly higher in susceptible strains but contents of N-acetyl glucosamine, a hydrolysate of chitin, were about 2 times higher in resistant ones.
李奎承,崔鍾佑,趙丁禮,金文圭 충남대학교 농업과학연구소 1992 농업과학연구 Vol.19 No.2
In order to use organic resource like a compost 44 samples of paper mill sludge were collected from each paper manufacturer. Some metal and PCBs(polychlorinated biphenyls) contents in/on sludge were investigated and metal contents of soil treated with kraft and paper board sludge were checked in contrast io the non treated soil. The staple results were the same as followings. 1. Average contents of Pb in sludge ranged 14.5-25.7 ppm, and Al, Cd., Cu, Fe, Zn were similar concentration to unpolluted soil. Also, Cr and Hg were not detected from any sludge sample. 2. PCBs contents in sludge of kraft. paper board and news print paper were determined. Less than 10 ppb of PCB_s contents were detected from kraft and paper board sludges, but a little more was found in sludge of news print paper. 3. Some metal contents in sludge treated soil were much similar to non-treated soil and the concentration also were ranged under the average of unpolluted natural soil. 4. From We results paper mill sludge seemed to be no harmful effects to soil.