수생조류에서 분리한 대장균의 항균제 내성 및 Tetracycline 내성인자의 분포

조재근,이상민,김기석,Cho, Jae-Keun,Lee, Sang-Min,Kim, Ki-Seuk 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3

One hundred and sixty nine Escherichia (E.) coli strains isolated from fecal samples of aquatic birds in Geumho river basin and Dalseong park were tested by agar dilution method to determine their susceptibility patterns to 14 antimicrobial agents. The distribution of tetracycline resistance determinants (tetA, tetB, tetC, tetD and tetE) were also examined by PCR in 76 tetracycline-resistant ($TC^r$) E. coli isolates. The high resistance was observed in tetracycline, cephalothin and ampicillin (45.0~36.7%). Resistance of E. coli isolates derived from Dalseong park to tetracycline, cephalothin, ampicillin and streptomycin (65.7~44.8%) were significantly higher than those isolated from Geumho river basin (31.4~14.7%). About seventy percent (70.4%) of the strains isolated were resistant to one or more drugs tested. Thirty (39.5%) of 76 $TC^r$ E. coli isolates which were resistant to one or more drugs transferred all or a part of their resistance patterns to the recipient strain of E.coli J53 by conjugation. All of $TC^r$ E. coli isolates contained at least one or more of 5 tet genes examined. The most common genes found in these isolates were tetA (60.6%) and followed by tetB (7.9%) and tetC (1.3%). However, tetD and tetE were not found in any of the isolates tested. Twenty one (27.6%) of $TC^r$ E. coli isolates had two determinants, tetA/tetB (20 strains), tetA/tetC (1 strain). And two strains (2.6%) contained three determinants (tetA/tetB/tetC).

후두암 환자에서 경구강 레이저 성문절제술 후 음성 복원 수술

조재근,손영익,Cho, Jae Keun,Son, Young-Ik 대한후두음성언어의학회 2013 대한후두음성언어의학회지 Vol.24 No.2

The cure rates for early stage laryngeal cancer are similar between laser cordectomy and radiation therapy. As well as the survival outcome, one of the main measures of success in treatment of early laryngeal cancer is voice outcome. Many studies have demonstrated that laser cordectomy and radiation therapy to be equivalent with regard to vocal outcome, whereas others favor radiation. Although such as somewhat disadvantages of voice outcome, laser cordectomy still remains a valid option. Since the patients who treated with laser may benefit from additional phonosurgery to improve postoperative vocal outcome. In this article, we reviewed the techniques of phonosurgery which can be used for laryngeal reconstruction after laser cordectomy. The indications for using each technique are discussed, with particular attention paid to functional outcomes following these reconstructive efforts.

설계사무소 운용을 위한 Server기반 BIM 통합정보시스템 프레임워크 개발

조재근,김연수,마영균,Cho, Jae-Keun,Kim, Yeon-Soo,Mha, Young-Kyun 한국BIM학회 2013 KIBIM Magazine Vol.3 No.2

In the architectural design industry recent BIM system was introduced to the vast amount of information generated by the process, who need it and require the need to manage the construction of information related to production, storage, delivery. In addition, for the project to perform the work involved collaboration between organizations is essential. The purpose of this study was to BIM standard for the system to effectively utilize BIM technology and development of the BIM business support collaboration system, to establish a BIM application environment, a variety of information in the field of architectural design that can be used practically efficient transcription.

지역사회 내 일반인 및 동물에서 methicillin 내성 Staphylococcus aureus의 유전학적 특성

조재근,김진현,성명숙,김기석,Cho, Jae-Keun,Kim, Jin-Hyun,Sung, Myung-Suk,Kim, Ki-Seuk 대한수의학회 2011 大韓獸醫學會誌 Vol.51 No.4

Methicillin-resistant Staphylococcus (S.) aureus (MRSA) is one of the most important nosocomial pathogens worldwide and the emergence of this strain has become a major clinical problem. In this study, we investigated the prevalence of MRSA and their genetic characteristics in 69 S. aureus isolated from humans and animals. In human isolates, higher antimicrobial resistance rates were observed against penicillin (80.6%), followed by erythromycin (11.9%) and tetracycline (9.0%). All of them were susceptible to clindamycin, enrofloxacin, novobiocin, pirlimycin, trimethoprim/sulfamethoxazole and vancomycin. The resistance patterns in animal isolates were similar to those of human isolates. Two (2.9%) MRSA strains were isolated from human (n = 1) and animal (n = 1), and these isolates were confirmed as carrying the mecA gene. One isolate originating from human was resistant to 7 drugs and the other isolate derived from animal was resistant to 11 drugs. Staphylococcal cassette chromosome mec (SCCmec) variant IIIB was identified in animal isolate but SCCmec type of an isolate from human was not exactly determined. Two MRSA isolates showed unrelated PFGE pattern between them. Our results indicated although the frequency of MRSA isolates from humans and animals was low, a continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSA in the community. To our knowledge, this is the first time that SCCmec type variant IIIB was detected from animals in Korea.

개의 외이도에서 분리한 포도상구균의 항생제 내성 및 병독성 유전자

조재근,이정우,김정옥,김정미,Cho, Jae-Keun,Lee, Jung-Woo,Kim, Joung-Ok,Kim, Jeong-Mi 한국동물위생학회 2022 韓國家畜衛生學會誌 Vol.45 No.3

The aim of this study was to investigate the prevalence of antimicrobial resistance and virulence factors in staphylococci isolated from canine otitis externa. A total 295 causative microorganisms were isolated. The most common isolated species were Staphylococcus (S) pseudintermedius (94 isolates) followed by Pseudomonas aeruginosa (60 isolates), S. schleiferi (25 isolates), Escherichia coli (23 isolates) and Proteus mirabilis (20 isolates). Staphylococci isolates were showed high resistance to penicillin (78.6%), erythromycin (55.9%), tetracycline (52.4%), clindamycin (51.7%) and ciprofloxacin (42.8%). Of the 145 staphylococci isolates, 49 (33.8%) methicillin-resistant staphylococci (MRS) were observed, distributed among S. pseudintermedius (n=34), S. schleiferi (n=6), S. epidermis (n=4), S. hominis (n=2), S. aureus, S. caprae and S. saprophyticus (n=1, respectively). Forty-three (87.8%) of 49 MRS and 10 (10.4%) of 96 methicillin-susceptibility staphylococci harbored mecA gene. About 80% of MRS were multidrug-resistant with resistance to at least one antibiotic in three or more antibiotic classes. Resistance genes blaZ (93/114, 81.5%), ermB (35/81, 43.2%), ermC (3/81, 3.7%), aacA-aphD (50/54, 92.5%), tetM (69/76, 90.7%) and tetK (6/76, 7.8%) were detected among resistant isolates. Virulence factors genes lukF and lukS were found in 100%(145/145) and 43.4%(63/145), respectively. Genes encoding ermA, eta, etb and tsst were not detected. To the best of our knowledge, this is the first study which investigated for the presence of genes encoding antimicrobial resistance and staphylococcal toxins in staphylococci isolated from canine otitis externa. A continuous monitoring and surveillance program to prevent antimicrobial resistance in companion animals is demanded.

닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이

조재근,손규희,김경희,김정미,박대현,이정우,Cho, Jae-Keun,Son, Kyu-Hee,Kim, Kyung-Hee,Kim, Jeong-Mi,Park, Dae-Hyun,Lee, Jung-Woo 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.3

The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

대구지역 동물병원에서 입원중인 개와 고양이로부터 분리된 항생제 내성 대장균

조재근,김정미,김환득,김경희,Cho, Jae-Keun,Kim, Jeong-Mi,Kim, Hwan-Deuk,Kim, Kyung-Hee 한국동물위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.3

This study was carried out to investigate the antimicrobial resistance profiles and resistance genes in 62 Escherichia coli isolated from dogs and cats hospitalized at animal hospitals in Daegu. E. coli isolates showed high resistance to nalidixic acid (46.8%) and ampicillin (45.2%). Resistance to the other antimicrobial agents was less than 30%, and no resistant isolates were detected for imipenem and amikacin. Of the 28 ampicillin-resistant isolates, TEM and CTX-M genes were detected in 16 (57.1%) and 11 (39.3%), respectively. The aadA gene was found in 4 (26.7%) of 15 gentamicin-resistant isolates, and strA-strB gene was found in 10 (66.7%) isolates. The sul I and sul II genes were detected in 11 (61.1%) and 14 (77.8%) of 18 trimethoprim/sulfamethoxazole-resistant isolates, and tetB gene in 9 (81.8%) of 11 minocycline-resistant isolates, and cmlA gene in 2 (22.2%) of 8 chloramphenicol-resistant isolates. The qnrB and qnrS genes were found in 3 (10.3%) and 1 (3.4%) of 28 nalidixic acid-resistant isolates, respectively. Whereas, none of the SHV, CMY-2, tetA, dfr Ia and dfr VII, and qnrA genes were found. Our results show a wide variety of resistance genes in E. coli isolates from dogs and cats. This study also represents the first report of qnrB and qnrS gene producing E. coli isolates from dogs in republic of Korea.

대구지역 개와 고양이에서 분리된 Staphylococcus pseudintermedius의 fluoroquinolone 내성

조재근 ( Jae-keun Cho ),김정미 ( Jeong-mi Kim ),김환득 ( Hwan-deuk Kim ),김경희 ( Kyung-hee Kim ),양창렬 ( Chang-ryoul Yang ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.1

The purpose of this study was to investigated the fluoroquinolone (FQ) resistance and presence of gyrA and grlA gene in 87 Staphylococcus pseudintermedius isolates obtained from clinical samples of dogs and cats. Also, the profiles of FQ resistance compared with methicillin resistant S. pseudintermedius (MRSP) isolates. FQ resistance was observed for enrofloxacin (41.4%), ciprofloxacin (39.1%), norfloxacin (36.8%), ofloxacin and levofloxacin (32.2%, respectively), and moxifloxacin (31.0%). Thirty-eight (43.7%) of 87 S. pseudintermedius isolates were resistant to more than one FQ. Twenty-six (64.5%) of 38 FQ resistant isolates were resistant to all the six FQ tested. Of 38 FQ resistant isolates, gyrA gene was detected in all isolates but grlA gene was not found. Moreover, 19 MRSP isolates were resistant to enrofloxacin (63.2%), ciprofloxacin (57.9%), norfloxacin (52.6%), and ofloxacin, levofloxacin and moxifloxacin (47.4%, respectively). FQ resistance were highly prevalence in S. pseudintermedius isolates from dogs and cats. Our results emphasize the prudent use of antimicrobial agents to companion animals is necessary for prevent antimicrobial resistance.

개와 고양이에서 분리한 Pseudomonas aeruginosa의 항생제 내성

조재근 ( Jae-keun Cho ),김정미 ( Jeong-mi Kim ),김경희 ( Kyung-hee Kim ),임현숙 ( Hyun-suk Lim ),양창렬 ( Chang-ryoul Yang ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.1

The aim of this study was to investigate the antimicrobial resistance among Pseudomonas (P.) aeruginosa isolated from dogs and cats. A total of 45 (6.2%) P. aeruginosa was isolated from 710 dogs and 21 cats with clinical signs. Resistance to one or more of the antimicrobials tested was observed in 26 (57.8%) P. aeruginosa. Resistance to cefepime was the most frequent (44.4%), followed by ofloxacin (22.2%), levofloxacin (17.8%), norfloxacin (8.9%), ciprofloxacin (6.7%), ceftazidime, aztreonam, colistin, polymixin B and gentamicin (4.4%, respectively), while resistance to piperacillin/tazobactam, imipenem, tobramycin and amikacin was 2.2%, respectively. All isolates were susceptibility to doripenem and meropenem. Antimicrobial susceptibility testing should be a crucial step in selection of appropriate antimicrobial therapy in veterinary medicine. Also, the prudent use of antimicrobials and continuous monitoring for companion animals are required.

낙동강과 금호강에서 분리된 광범위 베타 락탐 분해효소 생성 Escherichia coli 내 항균제 내성 및 integrons의 분포

조재근 ( Jae Keun Cho ),김환득 ( Hwan Deuk Kim ),권순효 ( Soon Hyo Kwon ),김진현 ( Jin Hyun Kim ),장성일 ( Sung Il Jang ),박최규 ( Choi Kyu Park ),김기석 ( Ki Seuk Kim ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.1

This study was conducted to investigate the antimicrobial resistance, presence of β-lactamase genes and integrons in 83 ESBL-producing Escherichia coli isolated from Nakdong river and Geumho river in Daegu. Among the β-lactam antimicrobials, all isolates were resistant to ampicillin, cephalothin, cefamandole and cefotaxime, followed by piperacillin (98.8%), ampicillin/sulbactam (86.7%), aztreonam (60.2%) and cefepime (59.0%), whereas resistance to piperacillin/tazobacram, ticarcillin/clavulanic acid and cefoxitin was less than 30%. Many of the ESBL-producing Escherichia coli were also resistant to non-β-lactams antimicrobials such as nalidixic acid (83.1%), sulfonamides (72.3%), ciprofloxacin (62.7%) and gentamicin (38.6%). All isolates showed resistance to seven or more antimicrobial agents.The most frequently detected gene was blaTEM+CTX-M (49.4%), followed by blaCTX-M (27.7%), blaTEM (6.0%) and blaOXA (1.2%). But blaSHV was not found. Class 1 integrons were found in 61.4% (51 isolates) of isolates, however, class 2 and 3 integrons were not detected. The results showed water from Nakdong river and Geumho river is contaminated with ESBL-producing E. coli isolates. These results suggest the need for further investigation of antibiotic resistant bacteria to prevent public health impacts in the water environment.