Saccharomyces cerevisiae에서 Pseudoalteromonas carageenovora 유래 Arylsulfatase 유전자의 표층 발현

조은수,김현진,정소아,김정환,김연희,남수완,Cho, Eun-Soo,Kim, Hyun-Jin,Jung, So-A,Kim, Jeong-Hwan,Kim, Yeon-Hee,Nam, Soo-Wan 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

P. carageenovora 유래 arylsulfatase 유전자(astA)의 효모표면발현 plasmid pCTAST(7.1 kb)를 구축하여 S. cerevisiae EBY100에 형질전환하였고, 선별된 형질전환체들을 YPDG 배지에서 배양했을 때 4-methylumbelliferyl sulfate를 분해하여 형광을 보였다. 이는 효모에서 arylsulfatase가 활성형으로 생산되었음을 의미하였다. S. cerevisiae EBY100/pCTAST를 플라스크 배양했을 때 arylsulfatase 활성은 galactose가 완전히 소모된 배양 48시간째 최대 활성인 1.2 unit/mL로 나타났으며 배양 상등액에서는 활성이 나타나지 않았다. 효모 S. cerevisiae의 세포표면에서 발현된 재조합 arylsulfatase로 제조된 agarose를 agar와 시판용 agarose와 함께 ${\lambda}DNA$ HindIII marker를 사용하여 DNA 전기영동 성능을 비교 실험한 결과, agar보다는 재조합 arylsulfatase 처리로 제조한 agarose가 이동성이나 분리능에서 우수하였으며, 시판용 agarose와 비교하여 이동성이나 분리능이 유사한 결과를 확인할 수 있었다. 본 연구의 결과, 효모 S. cerevisiae의 세포표면에서 발현된 재조합 arylsulfatase 효소를 이용하여 한천으로부터 전기영동용 고순도 친환경적인 agarose 생물 생산 공정에 적용 가능함을 알 수 있었다. In this study, the arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was expressed on the cell surface of S. cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The constructed plasmid, pCTAST (7.1 kb), was introduced to S. cerevisiae EBY100 cell, and yeast transformants on YPDG plate showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When S. cerevisiae EBY100/pCTAST was grown on YPDG medium, the arylsulfatase activity of cell pellet reached about 1.2 unit/mL, whereas no extracellular arylsulfatase activity was detected. The DNA ladder in agarose prepared from agar by this recombinant arylsulfatase showed similar resolution and migration patterns with a commercial agarose. This results revealed that arylsulfatase expressed on the cell surface of S. cerevisiae could be applicable to the economic production of electrophoretic-grade agarose.

유전자조작, 균주분리 Saccharomyces cervisiae에서 Pseudoalteromonas carapeenovora 유래 Arylsulfatase 유전자의 표층 발현

조은수 ( Eun Soo Cho ),김현진 ( Hyun Jin Kim ),정소아 ( So A Jung ),김정환 ( Jeong Hwan Kim ),김연희 ( Yeon Hee Kim ),남수완 ( Soo Wan Nam ) 한국미생물생명공학회 2009 한국미생물·생명공학회지 Vol.37 No.4

농촌마을 색채경관 개선을 위한 디자인 프로세스 연구

조은수(Cho, Eun-Soo),채민규(Chae, Min-Kyu) 한국실내디자인학회 2024 한국실내디자인학회논문집 Vol.33 No.1

Rural villages in Korea are forming heterogeneous landscapes that do not harmonize with the surrounding natural environment due to the overuse of highly saturated colors. The factor in this phenomenon is the housing improvement project of the Saemaul Movement, which was promoted nationwide in the 1970s to modernize underdeveloped rural and fishing areas. As a result, the roof color of Korea’s rural villages is still perceived as being out of harmony with the surrounding natural landscape and a fundamental solution to the color problem in rural villages has still not been found. Accordingly, the purpose of this study is to recognize the problems of color landscapes in rural villages in Korea that have not been resolved for a long time and to propose a color design process for practical improvement of color landscapes in rural villages. This process consists of 7 steps. In 「Research」, the concept of ‘main view points’ is utilized during field investigation, and In 「Analysis」, color analysis from various viewpoints was possible. 「Concept」 and 「Color Design」 presents standards for setting the direction of color design and color range, 「Guidelines」 presents detailed color guidelines. 「Simulation」 are used to determine whether the product is in harmony with the surroundings. 「Management and Utilization Plan」 presents a plan for systematic management and establishment of the color landscape after the end of the color landscape plan. The conclusion of the study is as follows. First, when planning the color landscape of a rural villages, the relationship with the background and adjacent objects must be considered. Second, it is essential to reflect major viewpoints when planning a color landscape. Third, a systematic color landscape management plan is needed even after the color landscape plan is completed. This process will be used as a helpful process to improve the color of rural villages in Korea.

베트남, 중국 결혼이주여성의 건강문해력 관련 요인

조은수(Cho, Eun Soo),양숙자(Yang, Sook Ja) 한국보건간호학회 2020 韓國保健看護學會誌 Vol.34 No.2

Purpose: This descriptive correlational study examined the acculturation, health literacy, and factors affecting the health literacy of Vietnamese and Chinese female marriage immigrants. Methods: The sample consisted of 90 Vietnamese and 89 Chinese female marriage immigrants who participated in Multicultural Family Support Centers’ programs in metropolitan city A. The health literacy was measured by HLI-FMI (Health Literacy Index for Female Marriage Immigrants), acculturation was measured using the Acculturation Index for female marriage immigrants. Descriptive statistics, t-test, Chi-square, one-way ANOVA, Scheffé test, Pearson correlation coefficient, and multiple linear regression were performed for data analysis. Results: The Chinese group (M=9.80±2.72) showed a significantly higher health literacy than the Vietnamese group (M=8.07±3.57). The factors affecting health literacy among Chinese were the length of residence (β=.442 p<.001), and adaptation to Korean culture (β=.381, p=.007). Conclusion: To develope graded education programs for promoting the health literacy of Chinese and Vietnamese female marriage immigrants, the length of residence can be considered. Continuously providing a support system to help them adapt to Korean culture can also have a positive effect on health literacy.

Saccharomyces cerevisiae와 Pichia pastoris에서 Bovine Pancreatic Deoxyribonuclease I의 과발현과 특성

조은수 ( Eun Soo Cho ),김정환 ( Jeong Hwan Kim ),윤기홍 ( Ki Hong Yoon ),김연희 ( Yeon Hee Kim ),남수완 ( Soo Wan Nam ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.4

본 연구에서는 S. cerevisiae와 P. pastoris에서 bovine pancreatic (bp-) DNase I의 과발현과 재조합 DNase I의 특성을 조사하였다. bp-DNase I 유전자는 GAL10 promoter, MFα, GAL7 terminator 사이에 삽입하여 재조합 plasmid인 pGAL-MFα-DNaseI (6.4 kb)를 구축하였다. 그리고 bp-DNase I 유전자를 AOX1 promoter, MFα, AOX1 terminator에 삽입하여 재조합 plasmid인 pPEXI (8.8 kb)를 구축하였다. 재조합 plasmid인 pGAL-MFα-DNaseI과 pPEXI를 각각 S. cerevisiae와 P. pastoris 숙주세포에 형질전환시켰다. 형질전환된 효모세포들을 galactose와 methanol 배지에서 30oC, 48시간 배양하면 bp-DNase I은 대부분이 배양 상등액으로 과발현되었다. P. pastoris 형질전환체는 배양 상등액에서 45.5 unit/mL의 DNase I 활성을 보였으며, 반면에 S.cerevisiae 형질전환체는 37.7 unit/mL의 DNase I 활성을 보였다. 또한 DNA 분해 특성을 조사한 결과, P. pastoris 재조합 DNase I으로 기질 DNA(calf thymus)를 처리하였을 때 1분 이내 DNA가 분해되는 것을 확인할 수 있었으며 이는 상업용 bp-DNase I과 S. cerevisiae 재조합 DNase I으로 처리했을 때보다 빠른 분해 패턴을 보였다. In the present study, we investigated the overexpression and characterization of bovine pancreatic (bp)- DNase I in Saccharomyces cerevisiae and Pichia pastoris. The bp-DNase I gene was fused in frame with the GAL10 promoter, MFα, and GAL7 terminator sequences, resulting in the plasmid, pGAL-MFα-DNaseI (6.4 kb). Also, the bp-DNase I gene was fused in frame with the AOX1 promoter, MFα, and AOX1 terminator sequences, resulting in the plasmid, pPEXI (8.8 kb). The recombinant plasmids, pGAL-MFα-DNaseI and pPEXI were introduced into S. cerevisiae and P. pastoris host cells, respectively. When the transformed yeast cells were cultured at 30oC for 48 h in galactose or methanol medium, bp-DNase I was overexpressed and the most of activity was found in the extracellular fraction. P. pastoris transformant activity showed 45.5 unit/mL in the culture medium at 48 h cultivation, whereas S. cerevisiae transformant revealed 37.7 unit/mL in the extracellular fraction at 48 h cultivation. The enzymatic characteristics, such as DNA cleavage and half life were investigated. Treatment of the recombinant DNase I from P. pastoris induced degradation of the calf thymus DNA within 1 minute, and this DNA degradation rate was higher than that of commercial bp-DNase I (SIGMA) and the recombinant DNase I from S. cerevisiae.

The Characteristic of Reward in Computer Assisted Learning

Eun Mo Yeon(연은모),Sun-Young Lee(이선영),Yoonkyung Chung(정윤경),Eun Soo Cho(조은수),Soongoo Kwon(권순구),Hun Jeon(전훈),Kye-Hyeng Lee(이계형),Sung-Hyun Yoon(윤성현),Yeonhee So(소연희),Sung-il Kim(김성일) 한국HCI학회 2008 한국HCI학회 학술대회 Vol.2008 No.2

Computer Assisted Learning (CAL) is quite different from in many aspects. CAL provides individualistic learning environment and facilitates autonomy of the learner. Thus the learners who uses CAL program has more sense of control and engages in more strategic learning than conventional learning environment. In this experiment, we used KORI (KORea university intelligent agent) which is a new type of ITS adopting TA (Teachable Agent) that fosters learning by teaching. So, we investigated the critical motivational factor that have influences in CAL learning and the effects of reward in CAL are another area of our interest. Thus, we divided two conditions that presence of reward and absence of reward. The 174 elementary school students(5th) were participated and they are randomly assigned the one of the reward conditions. Before entering the experimental instruction, all participants measured about metacognition, self-efficacy and goal orientation questionnaire as independent variables. Then, Participants were instructed of method of using KORI program and asked to study for ten days with KORI program at least 20 minutes everyday in their home, about 10 days. After 10 days, they were rated interest and comprehension. Regression results suggest that regardless of the presence of reward, metacognition is a positive predictor in interestingness. It indicate that metacognitive skills are required in CAL learning situation irrespective of reward. But on comprehension in the absence of reward, only self- efficacy appeared to be a positive predictor. In the presence of reward, performance goal orientation showed as a negative predictor of comprehension, whereas self-efficacy was a positive predictor. This result suggest that presence of reward especially interferes learning process of performance goal orientation in CAL learning situation. It could be interpreted that reward interferes the learning process of performance goal orientation by debilitating intrinsic motivation.

CNN 기반 아보카도 숙성도 분류

정산아(San ah Jeong),조은수(Eun Soo Cho),정호기(Ho Gi Jung) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.11

Avocado, which is used in various diet foods, has been listed in the Guinness Book of World Records as the most nutritious fruit in the world as well as teenage superfoods. Avocado is a fruit that requires post-aging, so it was often difficult for consumers to know if post-aging was completed. Previously, research has been conducted to determine the maturity of avocados using electrodes. However, in this study, the goal is to implement an algorithm that determines whether avocados have matured when consumers photograph them using a camera. The dataset was actually collected by taking about 2600 photos of avocados in the categories of ripe, unripe, and rotten, and a convolutional neural network (CNN) based network was trained. As a result, it was confirmed that about 72% of the accuracy was shown.

효모 표층 Arylsulfatase에 의해 제조된 Agarose의 특성

김연희 ( Yeon Hee Kim ),남수완 ( Soo Wan Nam ),조은수 ( Eun Soo Cho ),김저환 ( Jeong Hwan Kim ) 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 2010 한국미생물·생명공학회지 Vol.38 No.4

Agar로부터 agarose 제조 시 유기용매를 이용해서 황을 제거하는 방법이 일반적으로 많이 사용되어진다. 하지만 agar의 황을 가수 분해하는 효소를 사용할 경우 agarose 제조 시공정과정을 획기적으로 간소화할 수 있다. 따라서 arylsulfatase로 agaropectin에서 황 제거를 통해 agarose로 바꾸는 공정은 간단하고 높은 수율을 얻을 수 있기 때문에 agarose 생산에 효율적으로 적용할 수 있다. 본 연구에서는 arylsulfatase를 세포표면에 발현하는 효모생촉매를 이용하여 제조한 agarose의 황 함량과 gel 강도를 측정하였다. 처리한 효모(효)소의 농도가 증가할수록 증가된 탈황 반응에 의해 황함량이 줄어들었고, 특히 35 unit/mL의 효소 농도로 처리하였을 때 황 함량은 0.2%까지 감소하는 것을 확인할 수 있었다. 황 함량을 가장 낮출 수 있는 최적 조건은 0.6% agar(Junsei) 용액에 효모 표층 arylsulfatase 35 unit/mL로 처리하고 40oC에서 3시간 반응시켰을 때 였다. 또한 1.0% DNA 전기 영동용 agarose의 gel 강도는 효모 표층 arylsulfatase 처리로 제조된 agarose의 경우 559.8±0.12로 상업적 agarose의 gel 강도(880.6±0.15 g/cm2) 보다는 낮았다. 따라서 효모 S.cerevisiae의 세포 표면에서 발현된 재조합 arylsulfatase 효소를 이용하여 agar로부터 전기영동용 agarose의 생산 공정에 적용 가능함을 알 수 있었다. Enzymatic hydrolysis of sulfate groups in agaropectin or agar simplifies the production process of high-quality or low sulfate-content agarose. This study was investigated that cell surface displayed arylsulfatase can be applied to desulfatation of agar for production of agarose. Sulfate content of agarose prepared by treatment of yeast surface-displayed arylsulfatase was decreased in a enzyme dosedependent manner. Especially, 35 unit/mL of yeast surface arylsulfatase attenuated sulfate content of agarose up to 0.2%. In the 0.6% agar(Junsei), 35 unit/mL enzyme treated at 40oC for 3 h showed the lowest content of sulfate. Therefore, this result was determined to be the optimal condition to desulfatation of agar for production of agarose. In addition, the gel strength of yeast surface arylsulfatase treated agar and commercial agarose were compared. Agarose prepared by treatment of yeast surface arylsulfatase showed 559.8±0.12 of gel strength, and it is a similar compared to the commercial agarose.

엔진 마운트의 동특성 해석 및 최적설계

임홍재(Hong Jae Yim),성상준(Sang Joon Sung),이제형(Je Hyung Lee),조은수(Eun Soo Cho),이상범(Sang Beom Lee) 한국소음진동공학회 2000 한국소음진동공학회 학술대회논문집 Vol.2000 No.1

Horseshoe Appliance에 의한 전치부 반대교합 치험예 : CASE REPORTS OF ANTERIOR CROSSBITE CORRECTION TREATED BY HORSESSHOE APPLIANCE

조은수,김수철,정규림 대한치과교정학회 1984 대한치과교정학회지 Vol.14 No.2

The present paper describes 4 clinical cases of Hellman dental age Ⅲ A group of functional Class Ⅲ malocclusion in which the horsehoe appliance was applied to obtain normal anteroposterior relationship between jaws. What is the most noteworthy in the result from the use of this appliance is that minimum down and backward rotation during backward repositioning of the mandible is noticed and the excellent retentive property in itself makers allowance for effective orthopedic correction in mixed dentition with anterior crossbite.