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돼지 유래 생체 조직의 이식재 활용을 위한 방사선 조사미생물 제어 평가
조으리,김정수,최종일,김재훈,성낙윤,송범석,김재경,박종흠,이주운 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.3
This study was compared microbiological safety with gamma-irradiated porcinetendon and skin, as materials for the development of xenografts to regenerate damaged tissuesand protect secondary contamination. The porcine tendon and skin were gamma-irradiated afterinoculation of bacteria and virus to evaluate irradiation sensitivity of microorganisms. The resultshowed that the porcine tendon and skin were not different on the sensitivity of microorganismsby gamma irradiation. Bacteria inoculated in the porcine tendon and skin were confirmed that E. coli was the D10 values of 0.32±0.082 and 0.25±0.1 kGy on tendon and skin, and B. subtilis was4.00±0.312 and 3.88±0.3 kGy on gamma irradiation, respectively. Moreover, Virus inoculatedin the porcine tendon and skin was observed that poliovirus (PV) was 6.26±0.332 and 6.88±0.3kGy, and porcine parvovirus (PPV) was 1.75±0.131 and 1.73±0.2 kGy and bovine viraldiarrhoea virus (BVDV) was 3.70±0.212 and 3.81±0.2 kGy on gamma irradiation, respectively. Virus showed higher resistance compared to bacteria on gamma irradiation, but was not detectedCPE (cytopathic effect) by virus both tendon and skin at 25 kGy, a standard dose recommendedfrom IAEA for sterilization of medical products. Therefore, These results were considered thatgamma irradiation could control effectively bacteria and virus to develop safe porcine xenograft,and apply same irradiation doses to all tissues including tendon and skin of porcine.
시판 유제품 중에서 분리된 젖산균의 감마선 조사에 의한생존 효과
이지혜,김재경,조으리,성낙윤,최종일,김재훈,송범석,박종흠,육홍선,이주운 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.2
This study was conducted with effect of lactic acid bacteria by gamma irradiation. Lactic acid bacteria were exposed to irradiation with a single absorbed dose of 1, 2, 3, 4, 5, 6, 8, and10 kGy. Possible lactic acid bacteria, including Lactobacillus paracasei KCTC 13169, Lactobacilluscasei KCTC 3109, Lactobacillus acidophilus KCTC 3140, Lactobacillus plantarum subsp plantarumKCTC 3103, Lactobacillus debruekii subsp bulgaricus KCTC 3635, Streptococcus thermophilusKCTC 3658 were selected. The radiation sesitivities of lactic acid bacteria were expressed as D10values. The D10 values of Lactobacillus paracasei, Lactobacillus casei, Streptococcus thermophilus,Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus debruekii were calculated as0.42, 0.51, 0.47, 0.90, 0.44, and 0.61 kGy, respectively. Results suggest that L. acidiphilus has thehighly resistant to gamma irradiation.
이종이식재 개발을 위한 감마선 조사 돼지 인대의미생물학적 안전성 및 인장강도
김정수,성낙윤,조으리,최종일,송범석,김재경,임윤묵,이주운,이광원,김태운,변명우,김재훈 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.1
This study was to determine the microbiological safety and tensile strength of gammairradiatedporcine tendon for the development of safe xenografts. Escherichia coli and Bacillus subtiliswere used as model pathogens and inoculated as 106~107 log colonies forming unit (CFU) g-1. As model virus from porcine, porcine parvovirus (PPV), bovine viral diarrhoea virus (BVDV) andpoliovirus were inoculated as 105~106 tissue culture infectious dose (TCID)50 g -1 into porcine skin. The D10 value of E. coli and B. subtilis was measured as 0.32±0.082 kGy and 4.0±0.312 kGy,respectively. Additionally, the D10 values of PPV, BVDV and poliovirus were also shown as 1.75±0.131 kGy, 3.70±0.212 kGy and 6.26±0.332 kGy, respectively. Gamma irradiation decreased thetensile strength of porcine tendon. Results indicate that microbiological safety of porcine tendoncan be improved significantly by gamma irradiation. However, further studies are needed toimprove the tensile strength of gamma-irradiated porcine tendon.