Helicobacter pylori 감염에 의한 위염의 병리조직학적 양상 및 박멸치료 후의 호전

조영일(Young Il Jo),박형석(Hyung Seok Park),송기호(Ki Ho Song),인행환(Haeng Hwan In),윤성욱(Seong Yuk Youn),김예리(Yei Lee Kim),이재동(Jae Dong Lee),진춘조(Choon Jo Jin),윤상애(Sang Ae Yoon) 대한내과학회 1998 대한내과학회지 Vol.54 No.2

N/A body and fundus. A signi6cant, positive correlation was noted between HP score and the score for inflammation, for activity, and for mucus depletion. After eradication of HP infection, significant improvement in activity, chronic inflammation, and mucus depletion was detected in responders with a successful HP eradication; the activity return to normal in follow-up, and chronic inflammation and mucus depletion was decreased significally in severity in responders(p<0.05%). No significant reduction in severity of gastritis and mucus depletion was detected in the non-responders with persistent HP infection following anti-HP triple therapy. Conclusion: The eradication of HP results in a rapid, significant histopathological improvement in the gastritis scores and epithelial damage in gastric mucosa.

호두 첨가가 청국장의 지방산 조성 및 관능적 특성에 미치는 영향

박화영,최희은,조영인,최웅규,Park, Hwa-Young,Choi, Hee-Eun,Jo, Young-In,Choi, Ung-Kyu 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.5

본 연구에서는 호두를 10%, 20%, 30%까지 첨가하여 제조한 청국장의 지방산 조성 및 관능적 특성을 확인하고자 하였다. 아미노태 질소는 호두의 첨가량이 많아짐에 따라 감소하는 것으로 나타났으나, 호두를 30% 첨가할 경우에도 청국장의 식품위생법상 기준인 280 mg% 이상은 유지하고 있어, 청국장의 법적기준에는 부합하는 것으로 확인되었다. 호두가 첨가된 청국장의 휘발성 염기질소는 대조구에 비해서 감소되었다. 총 유리아미노산의 함량은 대조구와 비교하여 호두 20% 첨가구까지는 유의적인 차이가 없었으나, 호두 30% 첨가 청국장에서 유의적으로 감소하였으며, 필수 아미노산의 함량도 유사한 패턴으로 감소하였다. 포화지방산의 함량은 호두의 첨가량에 반비례하여 유의적으로 감소하는 것으로 확인되었다. 총 불포화지방산의 함량을 확인한 결과, 호두의 첨가에 따른 청국장의 불포화지방산의 함량 증가를 확인할 수 있었다. 관능검사에서는 맛과 종합적 기호도의 경우 호두 10%와 20% 첨가구가 가장 좋은 것으로 나타났다. The aim of this study was to determine the changes in fatty acid composition and sensory characteristics of cheonggukjang containing walnuts at 0, 10, 20, or 30%. Amino nitrogen content was decreased with increasing content of walnuts. However, cheonggukjang containing 30% of walnuts met the standard of food sanitation act. Volatile basic nitrogen contents of cheonggukjang containing walnuts was lower than that of cheonggukjang containing no walnut. There was no significant difference in total amino acid content for cheonggukjang containing 0%, 10%, or 20% of walnuts. However, total amino acid content in cheonggukjang containing 30% of walnuts was significantly lower than that in cheonggukjang containing 0%, 10%, or 20% of walnuts. The content of essential amino acid in cheonggukjang containing 30% of walnuts was also significantly lower than that in cheonggukjang containing 0%, 10%, or 20% of walnuts. Saturated fatty acid content was decreased significantly from 16.7% to 3.6% when the content of was increased from 0% to 30%. Total unsaturated fatty acid contents was increased with increasing amounts of walnut added to cheonggukjang. Result of sensory evaluation revealed that the taste and overall quality of cheonggukjang made with 10~20% walnuts were significantly higher compared to those of ordinary cheonggukjang. These results suggest that cheonggukjang made with 10~20% walnuts could be developed as a novel fermented food.

건강인에서 소장의 감각능과 팽창도에 대한 선택적 5-HT4 수용체 부분 작용제 tegaserod의 효과

정정조 ( Jeong Jo Jeong ),최명규 ( Myung Gyu Choi ),조유경 ( Yu Kyung Cho ),전은정 ( Eun Jung Jun ),조영석 ( Young Seok Cho ),정우철 ( Woo Chul Chung ),이인석 ( In Seok Lee ),김상우 ( Sang Woo Kim ),최상욱 ( Sang Wook Choi ),정인 대한소화기기능성질환·운동학회 2004 Journal of Neurogastroenterology and Motility (JNM Vol.10 No.1

목적: 위장관의 내장 감각능 이상은 기능성 소화불량증의 중요한 병인으로 알려져 있으나 위장관의 감각을 지각하는 기전은 정확히 알려져 있지 않다. 세로토닌은 위장관의 운동과 감각능을 조절하는데 중요한 역할을 한다. 저자는 5-hydroxytryptamine-4 (5-HT4) 수용체 부분 작용제인 tegaserod가 건강인에서 소장의 감각능과 팽창도에 어떠한 영향을 주는지 알아보고자 하였다. 대상 및 방법: 건강 지원자 16명을 대상으로 위약이나 tegaserod (6 mg 하루 2회) 두가지 약물중 한 약제를 단일 맹검, 무작위 순서로 3일간 전투약한 후 검사를 시행하였다. 십이지장 3부에 최대 용적 120 mL의 풍선이 부착된 검사관을 위치시킨 후 바로스타트에 연결하여 소장의 팽창도 및 소장의 감각능을 평가하였다. 풍선의 용적을 단계적으로 증가시키면서 최초 인지 용적(처음으로 감각을 느끼는 용적), 최대 인내 용적(불편감/통증으로 참기 힘들때의 용적)을 기록하고 압력 변화를 관찰하여 소장 팽창도를 계산하였다. 소장 감각능은 20 mL, 40 mL, 60 mL의 3가지 팽창 자극을 무작위 순으로 3회씩 주어 피험자가 느끼는 감각을 시각적 선형척도에 기록하게 하여 평가하였다. 고칼로리 유동식인 엔슈어를 지속적으로 소장에 주입하면서 다시 무작위 순서로 3가지의 팽창자극을 주면서 피험자의 감각을 측정하였다. 결과: Tegaserod 투여군의 소장 팽창도는 2.59±0.74 mL/mmHg로 위약 투여군의 소장 팽창도 2.25±0.42mL/mmHg에 비해 유의한 차이가 없었다. tegaserod 투여군과 위약 투여군 사이에 최초 인지 용적의 차이는 없었지만 최대 인내 용적은 tegaserod 투여군에서 55.7±12.9 mL로 위약투여군 41.7±7.3 mL에 비해 유의하게 높았다( p<0.05). 20,40, 60 mL의 팽창자극을 하였을 때 오심, 팽만감, 불편감/통증 및 총 감각 지수는 tegaserod 투여군과 위약 투여군 사이에 차이가 없었다. 유동식을 주입하면서 팽창자극을 주었을때에도 두 군간에 팽창 자극에 대한 감각 점수에는 유의한 차이가 없었다. 결론: Tegaserod군과 위약군 사이에 빠른 위상성 팽창 자극에 대한 감각의 차이는 없었으나 단계적 팽창 자극에 대한 최대 인내 용적이 tegaserod군에서 위약군에 비해 유의하게 높아 tegaserod가 소장의 감각을 완화시키는 것으로 추정할 수 있다. Backgr ound/Aims: Disturbances in visceral perception contribute to postprandial symptoms in patients with functional dyspepsia. However, the mechanism of perceiving sensation has not been clearly defined. Our aim in this study was to evaluate the effect of tegaserod on compliance and visceral perception in the proximal small intestine. Methods: Sixteen healthy subjects were included in this study. Subjects underwent single blinded randomization to either a placebo group or a regimen group of 3-day pretreatment with tegaserod (6 mg bid). Thirty minutes after the insertion of a balloon-equipped, double-lumen tube into the duodenum, we sequentially measured the compliance and maximal tolerable volume to stepwise balloon distension. The maximum volume of the balloon was 120 mL, and an electronic barostat was used to maintain a constant volume within the balloon. Sensory function was assessed by grading accompanying symptoms of nausea, fullness, and abdominal discomfort/pain during random-ordered isovolumetric ditension of the balloon at volumes of 20 mL, 40 mL, and 60 mL. We made additional assessments on sensory function by slightly modifying the above method of randomized balloon distension to include continuous infusion of liquid meal into the tube. Results: There was no significant difference in compliance between the two groups. Maximal tolerable volume was significantly larger in the tegaserod group than in the placebo group (55.7±12.9 mL vs. 41.7±7.3 mL, p<0.05). Perceived sensations measured using the visual analogue scale during random ordered rapid phasic distension (20, 40, 60 mL) did not reveal differences between the two groups. Conclusions: Tegaserod had no effect on perceived sensation during rapid phasic distension, but it did increase the maximal tolerable volume during stepwise distension. These results suggest that tegaserod reduces the sensitivity to small bowel distension in healthy subjects. (Kor J Neur ogastr oenter ol Motil 2004;1:35-42)

의성 자두 대석조생, 후무사, 추희의 영양성분과 항산화 활성 비교 분석

엄정영(Jung-Young Eom),김현진(Hyun-Jin Kim),최상헌(Sang-Hun Choi),서현주(Hyun-Ju Seo),전익조(Ik-Jo Chun),성지혜(Jeehye Sung),임재환(Jae Hwan Lim),권인숙(In-Sook Kwun),조영은(Young-Eun Cho) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.6

본 연구는 동양계 자두 품종 중에서 의성 자두인 대석조생, 후무사, 추희 품종의 영양성분 및 항산화 활성을 평가하였다. 총 식이섬유는 대석조생> 후무사> 추희 순으로 대석조생이 가장 높았다. 칼슘 함량에서는 대석조생> 추희> 후무사 순으로 대석조생이 칼륨, 칼슘, 인의 함량이 모두 높은 것으로 나타났다. 총 폴리페놀 함량은 추희, 대석조생이 유의적으로 높았고 총 플라보노이드 함량은 대석조생이 유의적으로 가장 높았다. 항산화 활성은 대석조생> 후무사> 추희 순으로 대석조생이 가장 높았다. 따라서 동양계 자두인 의성 자두는 식이섬유 함량이 높고 열량이 낮으며, 높은 항산화 활성을 갖고 있어 다양한 건강 기능성 식품 소재로 활용도가 높을 것으로 사료된다. In this study, we investigated the chemical properties and antioxidant activities of Prunus salicina cultivars. We analyzed the nutritional ingredients, minerals, total polyphenols, total flavonoids, total fiber, and antioxidant activities of Prunus salicina Daeseok, Formosa, and Chuhui produced in Uiseong. There were no significant differences in the carbohydrate composition i.e., fructose, sucrose, and maltose between Prunus salicina Daeseok, Formosa, and Chuhui. Trans fat and cholesterol were not detected in any of the cultivars. The highest contents of P, K, and Ca were observed in the descending order of Prunus salicina Daeseok, Formosa, and Chuhui. In addition, the highest contents of total polyphenol and flavonoid were significantly detected in Prunus salicina Daeseok. Furthermore, the highest content of total fiber was observed in Prunus salicina Daeseok and Formosa. The highest antioxidant activities were observed in Prunus salicina Daeseok compared to Formosa and Chuhui. Taken together, these results suggest that Prunus salicina Daeseok produced in Uiseong could help develop antioxidant products.

B16F10 melanoma cell을 이용한 캐모마일(Matricaria chamomilla L.) 추출물의 미백 효과

조재범 ( Jae-bum Jo ),김명욱 ( Myung-uk Kim ),이은호 ( Eun-ho Lee ),김예진 ( Ye-jin Kim ),조은비 ( Eun-bi Cho ),강인규 ( In-kyu Kang ),조영제 ( Young-je Cho ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3

기능성 천연물 소재로서의 가능성을 검토하고자 캐모마일 추출물의 미백 효과를 조사하고 melanin 생성 반응에 관여하는 물질 억제에 대한 기전을 규명하고자 하였다. 캐모마일을 water와 60% ethanol을 이용하여 추출하였고, 얻어진 추출물을 phenolic농도별로 설정하여 tyrosinase 저해 활성을 알아보았을 때, water추출물의 경우 효과가 미비하였고, 60% ethanol 추출물에서는 농도 의존적으로 저해 활성이 나타내어 melanin 생성 저해 효과가 있을 것으로 판단되었다. 캐모마일 60% ethanol 추출물을 동결건조하여 얻어진 분말을 이용하여 B16F10 melanoma cell에 대한 세포 독성을 측정 결과, 75 μg/mL의 농도에서부터 독성이 관찰되어 농도 구간을 10, 25, 50 μg/mL으로 선정하였다. α-MSH로 자극한 B16F10 melanoma cell에서 melanin 생성량을 측정하여 캐모마일의 melanin 성성 억제 효능과 melanin 생성에 영향을 미치는 단백질인 tyrosinase, MITF, TRP-1, TRP-2의 단백질 발현 억제 효과를 알아본 결과, 캐모마일의 농도가 높아짐에 따라 농도 의존적으로 melanin 생성 함량이 감소하였고, tyrosinase, TRP-1, TRP-2, 등의 단백질 발현량 또한 감소하는 것을 확인할 수 있었다. 따라서 캐모마일 추출물은 B16F10 melanoma cell에서 melanin의 생성을 억제하고, melanin생성 관련 단백질발현을 억제하는 효과가 있음을 확인하였다. 위의 결과들로 인하여 캐모마일은 미백 기능성 식품 산업화를 위한 유용한 자원으로 활용 될 것으로 예상되며, 추후 산업적 응용을 위한 지속적인 연구가 진행되어야 할 것으로 판단되었다. Matricaria chamomilla L. has been used as a bath agent in Europe because of its sterilization effect on the skin. Flowers contain terpenes, flavonoids are effective in relieving inflammation. Matricaria chamomilla L. has been reported to have various drug efficacies such as sedation, anti-diabetic effect and anti-arthritic effect, but there is little research on the scientific efficacy of whitening effect. The purpose of this study was to examine the whitening effect of Matricaria chamomilla L. extract and to investigate the mechanism of inhibition of melanogenesis. The extracts were used to determine tyrosinase inhibitory activity. The tyrosinase inhibitory activity of extracts was ineffective for water extract but in 60% ethanol extract was shown in a concentration-dependent manner. B16F10 melanoma cell was measured using a powder obtained by lyophilization of 60% ethanol extract. The toxicity was observed at a concentration of 75 μg/mL. And concentration range was selected to be at most 50 μg/ mL. The effect of tyrosinase, MITF, TRP-1 and TRP-2 on the expression of melanin protein was investigated in melanoma cells of B16F10 melanoma cells. As a result, it was confirmed that as the concentration of the extract increased, the melanogenesis level decreased and the protein expression level also decreased in a concentration dependent manner. Therefore, it was concluded that Matricaria chamomilla L. extract inhibited melanogenesis in cells. Based on the above results, it is expected that it will be used as a useful basic data for industrialization of whitening functional food of Matricaria chamomilla L.

C57BL/6 마우스에서 기능성 샴푸 Bonogen의 양모 촉진 효과

홍진태(Jin-Tae Hong),이세라(Se-Ra Lee),김환희(Hwan Hee Kim),조영광(Young-Kwang Jo),백인정(In-Jeoung Baek),연정민(Jung-Min Yon),남상섭(Sang-Seop Nahm),곽동훈(Dong Hoon Kwack),이정은(Jung Eun Lee),이범준(Beom-Jun Lee),윤영원(Young-Won 한국독성학회 2006 Toxicological Research Vol.22 No.3

Bonogen shampoo is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effects of Bonogen shampoo on hair growth in an alopecia model of C57BL/6 mice. There were eight male and female experimental groups including distilled water (DW; negative control), a commercial shampoo [M], 3% minoxidil (MXD) and Bonogen shampoo (BNG). Dorsal skin hair of six-week-old mice was trimmed with an electric clipper carefully not to damage the skin. The next day, mice without skin scratch were selected, randomized and separated in 10 mice per group. The test compounds were topically treated with 0.15 ml per mouse on dorsal skin for 21 days daily and then washed thoroughly with DW. The hair regrowth was determined photographically at 0, 4, 7, 10, 15, 18, and 21 days and histologically at day 21. No clinical signs were observed in all mice. Although body weight was slightly increased in 3% MXD group than other groups, it was not significant. Hair regrowth began to be promoted after 14 days and appeared a distinct regrowth pattern in all animals by topical treatment of test compounds at 18 days. In particular, the topical treatment of bonogen shampoo or 3% MXD for 21 days to dorsal skin accelerated hair regrowth faster than DW or M shampoo. At 21 days, the hair regrowth promotion speed was in order of 3% MXD > BNG > M > DW. The bonogen shampoo or 3% MXD also promoted hair follicle elongation compared to the negative control. These results suggest that bonogen shampoo has hair growth promoting activities and may be useful for treatment of bald or alopecia.

호두의 화장품 기능성

Mee-Kyung Kim(김미경),Jin-Sung Kim(김진성),Bun-Sung Jo(조분성),Jeung-Hoan Kim(김정환),In-Cheol Lee(이인철),Myung-Sup Lee(이명섭),Young-Je Cho(조영제) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

호두과육으로부터 용매별로 추출한 추출액중의 phenolic compound 함량은 열수, 에탄올, 메탄올, 아세톤 추출물 순서로 24.3, 34.4, 32.5, 15.1 ㎎/g의 함량을 나타내어 에탄올 추출물의 phenolic compound 함량이 가장 높았으며, 인체에 무해한 추출 용매인 열수 및 에탄올 농도별 추출물의 phenolic compound 비교한 결과 60% 에탄올 추출물에서 34.7 ㎎/g과 열수 추출물에서 24.6 ㎎/g 함량의 phenolic compound를 나타내었다. 호두추출물의 항산화 활성 중 전자공여능의 경우 열수추출물에서 78.1%, 60% 에탄올추출물에서 80.6%의 활성을 나타내었으며, ABTS radical cation decolorization 측정에서는 열수추출물과 60% 에탄올추출물 각각 98.1%와 98.3%의 높은 항산화 활성을 나타내었다. Antioxidant protection factor (PF)의 경우는 열수추출물과 60% 에탄올추출물에서 각각 1.1±0.2 PF와 1.1±0.4 PF로 높지는 않았으며, TBARs inhibitory activity 측정에서는 열수추출물과 60% 에탄올추출물에서 각각 60%와 75%로 비교적 높은 항산화력을 가졌음이 확인되었다. 호두추출물의 항염증효과를 살펴본 결과 100 ㎍/㎖의 농도에서 50%의 NO발현이 억제되었고, western blot에 의한 iNOS 및 COX-2의 억제효과는 대조군에 비해 100 ㎍/㎖의 농도에서 10% 정도의 낮은 저해 효과를 나타내었다. Tyrosinase의 저해 활성의 경우 60% 에탄올추출물에서 43%의 저해율을 보였으며, 수렴효과의 경우 60% 에탄올추출물에서 55%의 억제효과를 나타내어, 호두추출물은 미백 및 주름개선용 화장품소재로서 개발이 가능하다고 판단되었다. The phenolic compounds of walnut extracts by various solvents were shown to be 24.3 ㎎/g in hot water, 34.4 ㎎/g in ethanol, 32.5 ㎎/g in methanol and 15.1 ㎎/g in acetone. In a comparison of phenolic compounds from hot water and different concentrations of ethanol, which are harmless, 60% ethanol extract and hot water extract were 34.7 ㎎/g, 24.6 ㎎/g. The electron donating ability (EDA) of walnut extracts in hot water and 60% ethanol were 78.1% and 80.6%. According to ABTS radical cation decolorization for antioxidant activity, hot water and 60% ethanol extract showed high antioxidant activities of 98.1% and 98.3%. Antioxidant protection factor (PF) were 1.1±0.2 PF and 1.1±0.4 PF in hot water and 60% ethanol extract. In TBARs inhibitory activity, each extract showed high antioxidant activities at 60% and 75%. Anti-inflammation effects of walnut extract were tested, and inhibition of NO was 50% in 100 ㎍/㎖ phenolics. Inhibitory activity against iNOS and COX-2 were shown, through Western blot, to be 10% in 100 ㎍/㎖ phenolics. Tyrosine inhibitory activity of 60% ethanol extract was 43%, and astringent effect of 60% ethanol extract was 55%. These results suggest that walnut extracts are suitable for functional cosmetics requiring skin-whitening and anti-wrinkle activity.

포스터 전시 : 하부위장관 6 ; 혈전성 혈소판 감소성 자반증을 동반한 출혈성 대장염 1예

조영석 ( Young Seok Cho ),채현석 ( Hiun Suk Chae ),정정조 ( Jeong Jo Jung ),김성수 ( Sung Soo Kim ),한석원 ( Suk Won Han ),이창돈 ( Chang Don Lee ),최규용 ( Kyu Yong Choi ),정인식 ( In Sik Chung ),선희식 ( Hee Sik Sun ) 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

약물치료에 반응을 하지 않았던 비폐쇄성 연하곤란 1 예

조영석(Young Seok Cho),최명규(Myung Gyu Choi),정우철(Woo Chul Chung),정정조(Jeong Jo Jeong),이인석(In Seok Lee),김상우(Sang Woo Kim),김성수(Sung Soo Kim),채현석(Hiun Suk Chae),한석원(Sok Won Han),정인식(In Sik Chung),박두호(Doo Ho Pa 대한소화기기능성질환·운동학회 2002 Journal of Neurogastroenterology and Motility (JNM Vol.8 No.1

Dysphagia is a frequent symptom which is observed in about 6% of all population. The most common causes of nonobstructive dysphagia are reported to be esophageal motility disorders, systemic disease and GERD. About 30% of GERD patients complain of dysphagia. Dysphagia in GERD patients is mostly due to peptic stricture but occasionally related to transient segmental esophageal motor disorder. A 42-year old male patient was admitted because of dysphagia and weight loss. He had renal transplantation 5 years ago. The results of esophagogastroduodenoscopic examination, esophagogram and esophageal manometry were normal. Treatment with proton pump inhibitor, prokinetic and anti-depressant was begun but the patient continued to complain of dysphagia. The result of 24 hour ambulatory pH monitoring revealed pathologic reflux. The results of 24 hour ambulatory esophageal manometry and video fluoroscopy were normal. After reassured, he didn`t complain of dysphagia. We report this case of nonobstructive dysphagia accompanied with GERD refractory to medical treatment in patient who had renal transplantation.(Korean Journal of Gastrointestinal Motility 2002;8:47-52)

수소취급 안전에 관한 연구

조영도(Young-Do Jo),김인찬(In-Chan Kim),권정락(Jeong-Rock Kwon),박기동(Ki-Dong Park) 한국가스학회 2013 한국가스학회 학술대회논문집 Vol.2013 No.5