담배 알카로이드 분획이 닭 간장 Succinate Dehydrogenase 에 미치는 영향 ( 1 )

조영동,안문환 ( Yong Dong Cho,Moon Hwhan Ahn ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.3

The effect of tobacco alkaloid fraction on SDH was studied. At low concentration of the alkaloid fraction, succinate dehydrogenase was activated whereas it was inhibited at high concentration of the alkaloid fraction. Inhibition of SDH by malonate was partially relieved by the alkaloid fraction.

인삼 사포닌의 생화학적 연구 ( ⅩⅤ )

주충노,조영동,구자현,김춘원,이송재 ( Chung No Joo,Yong Dong Cho,Ja Hyun Koo,Chung Won Kim,Song Jae Lee ) 생화학분자생물학회 1980 BMB Reports Vol.13 No.1

(1). When experimental rats were fed with fats either with ginseng saponin (0.05 ㎎/rat) or without the saponin, the blood serum lipids of both groups were found increased gradually on time course and no appreciable difference in the absorption patten between the two groups cood be observed. However, in cannulated rats, in which the secretion of bile and pancreatic juice into small intestine was impaired, the absorption of fats was greatly stimulated in the saponin fed (0.05 ㎎/rat) group suggesting that even the small amount of ginseng saponin greatly favoured for the lipid absorption in the absence or at the shortage of bile and pancreatic juice. (2). It was demonstrated that the highest radioactivity of blood lipid of ginseng administered rats appeared ealier (at 3 hrs.) than control (at 5 hrs.) after radioactive glycerol-tri (1-^(-14)C)-palmitate feeding. The value of radioactivity was also much higher in test group on time course after the radioactive fat feeding and the highest peak appeared earlier than that of control rats. In the 4-^(-14)C-cholesterol fed animals, the highest activity of both blood serum and liver lipids of ginseng administered rats appeared 6 hr. after the feeding but no significantly high radioactivity in control was observed during the experimental period. This results suggest that ginseng saponin stimulated fats and cholesterol absorption and transport. (3). The radioactivity-time course data of hepatic fatty acids of ginseng treated rats for two weeks prior to glycerol-tri (1-^(-14)C)-palmitate administration showed that the highest peak of the radio-activity appeared faster (3 hr. after the feeding) than that (5 hr, after) of control group. Furthermore the radioactivity of non-lipid fraction of the liver of test group was much higher than that of control group. In the liver of ginseng treated rats prior to 4-^(-14)C cholesterol administration, the highest was observed at 4 hr. after the isotope feeding while no appeciable radioactivity peak can be observed in control group during the experimental period. This suggests that the saponin stimulates fatty acid and cholesterol metabolism.

Biochemical Studies on Ginseng Saponins (XV) - The effect of ginseng saponins on the absorption of fats and cholesterol in rats.

주충노,조영동,구자현,김춘원,이송재,Joo, Chung-No,Cho, Yong-Dong,Koo, Ja-Hyun,Kim, Chung-Won,Lee, Song-Jae 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.1

1) 미량(한마리당 0.05 mg)의 인삼 사포닌을 투여한 흰쥐에서의 지방 흡수 양상은 대조군과 별로 큰 차이가 없었으나 삽관을 부착시켜 소장으로의 담즙과 췌장 분비액이 유입하지 못하도록 수술한 쥐에서는 미량의 인삼 사포닌도 지방 흡수를 크게 촉진시킨 것으로 관찰되었다. 2) 인삼 사포닌과 함께 구경으로 방사성지방을 투여한 쥐에서의 혈청지질의 최고 방사능은 투여한 후 3시간후에 관찰되는데 비하여 대조군의 경우는 5시간후에나 관찰되었다. 또한 방사능의 값도 시험군의 것이 훨씬 컸다. 간장의 지질도 시험군의 최고방사능관찰시기가 대조군보다 빠르고 컸다. 클레스테롤을 투여한 경우에서도 위와 같은 실험결과를 얻었다. 이와 같은 실험결과는 인삼사포닌이 지방이나 클레스테롤의 흡수 및 이동을 촉진하는 것으로 풀이된다. 3) 2주일간 인삼사포닌을 투여한 쥐에서 방사성지방을 투여한후 시간경과에 따른 방사능을 조사한 결과 시험군은 3시간 후, 대조군은 4시간 후에 각각 최고방사능이 관찰되었다. 또한 시험군은 바지방부분의 방사능도 대조군보다 훨씬 컸다. 방사능 클레스테롤을 투여한 쥐에서도 시험군은 5시간 후에 최고방사능이 관찰된데 비하여 대조군에서는 최고방사능 피이크가 실험시간 내에서는 관찰되지 않았다. 이와 같은 실험결과는 인삼사포닌이 지방산과 클레스테롤의 대사를 촉진함을 의미한다. (1). When experimental rats were fed with fats either with ginseng saponin (0.05 mg/rat) or without the saponin, the blood serum lipids of both groups were found increased gradually on time course and no appreciable difference in the absorption patten between the two groups could be observed. However, in cannulated rats, in which the secretion of bile and pancreatic juice into small intestine was impaired, the absorption of fats was greatly stimulated in the saponin fed (0.05 mg/rat) group suggesting that even the small amount of ginseng saponin greatly favoured for the lipid absorption in the absence or at the shortage of bile and pancreatic juice. (2). It was demonstrated that the highest radioactivity of blood lipid of ginseng administered rats appeared ealier (at 3 hrs.) than control (at 5 hrs.) after radioactive glycerol-tri (1-$^{14}C$)-palmitate feeding. The value of radioactivity was also much higher it test group on time course after the radioactive fat feeding and the highest peak appeared earlier than that of control rats. In the 4-$^{14}C$-cholesterol fed animals, the highest activity of both blood serum and liver lipids of ginseng administered rats appeared 6 hr. after the feeding but no significantly high radioactivity in control was observed during the experimental period. This results suggest that ginseng saponin stimulated fats and cholesterol absorption and transport. (3). The radioactivity-time course data of hepatic fatty acids of ginseng treated rats for two weeks-prior to glycerol-tri (1-$^{14}C$)-palmitate administration showed that the highest peak of the radio-activity appeared faster (3 hr. after the feeding) than that (5 hr. after) of control group. Furthermore the radioactivity of non-lipid fraction of the liver of test group was much higher than that of control group. In the liver of ginseng treated rats prior to 4-$^{14}C$-cholesterol administration, the highest was observed at 4 hr. after the isotope feeding while no appeciable radioactivity peak can be observed in control group during the experimental period. This suggests that the saponin stimulates fatty acid and cholesterol metabolism.

리기다소나무의 잎 추출액이 근단세포의 (根端細胞) 형태변화에 미치는 영향

김용옥(Yong Ok Kim),이호준(Ho Joon Lee),김은수(Eun Soo Kim),조영동(Young Dong Cho) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.1

N/A

암처리한 인삼엽의 Greening 과정에서의 Chlorophyll-protein Complex 형성에 대한 연구

박인호,홍영남,권영명,이순희,조영동,Park, In-Ho,Hong, Young-Nam,Kwon, Young-Myung,Lee, Soon-Hee,Cho, Yong-Dong 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.1

암처리(暗處理)한 인삼엽(人蔘葉)을 greening시키는 과정에서(광도 ; 2,000 lux연속광 ; 온도 $25^{\circ}C$;상대습도, 66%) 형성되는 chlorophyll-protein complex의 전기영동 패턴, 각(各) complex의 엽록소조성 및 흡수스펙트럼 양상을 조사하였다. 광하(光下)에서 자란 인삼엽(人蔘葉)의 thylakoid membrane을 5% SDS-PAGE로 분리하였을 때 6개의 chlorophyll-containing band를 볼 수 있었다(CP1a, CP1, LHCP1, LHCP2, LHCP3 complex, free pigment zone). Chlorophyll a-rich CP1 complex와 chlorophyll a/b-rich LHCP complex는 676nm와 670nm에서 각각 흡수극대를 나타냈으며, chl. a/b-rich LHCP complex는 652nm에서 minor한 peak를 나타내었다. 암처리 인삼의 greening과정에서 CP1 LHCP complex는 12시간의 광(光)처리후에 이미 형성되어 있었으며, CP1 complex의 chl. a/b ratio와 각 complex의 chlorophyll함량은 greening이 진행됨에 따라 점차 증가하는 경향을 보이고 있다. 이러한 결과는 광(光)에 의해 유도되는 CP complex분자의 생합성 및 기능적으로 활성을 갖는 PSI과 PSII의 형성이 광(光)처리 12시간내에 나타난 가능성을 시사한다. The formation and chlorophyll composition of chlorophyll-protein complexes were investigated in the etiolated ginseng leaves during greening under continuous white light(2000 lux, $25^{\circ}C$, 66% relative humidity). Six chlorophyll-containing bands (CP1a, CP1, LHCP1, LHCP2, LHCP3, free pigment zone) were separated by SDSPAGE from ginseng leaves(grown under light). Chlorophyll a-rich CP1 complex and chlorophyll a/b-rich LHCP complexes reveal their maximum absorption peaks at 676nm and 670nm, respectively. And chlorophyll a/b-rich LHCP complexes have another minor peak at 652nm. CPI and LHCP complexes have already formed in the etiolated ginseng leaves after 12 hr light treatment. And chlorophyll a/b ratio of CP1 complex and the percentages of chlorophyll contents of each complexes increased progressively during greening. These results suggest that the light-induced biosynthesis of CP complex molecules and the formation of functional PSI and PS II could occur within 12 hr of illumination in the etiolated ginseng leaves during greening.

경북 내성천의 하도 지형 환경 및 퇴적물 분석

이광률(Gwang Ryul Lee),조영동(Yong Dong Cho),김대식(Dae Sik Kim),김중석(Jung Suk Kim),정우헌(Woo Heon Jeong),조현진(Hyun Jin Cho),윤국현(Kuk Hyun Yun) 한국지역지리학회 2010 한국지역지리학회지 Vol.16 No.2

암처리한 인삼엽의 Greening 과정에서의 Chlorophyll - protein Complex 형성에 대한 연구

박인호,홍영남,권영명,이순희,조영동 ( In Ho Park,Young Nam Hong,Young Myung Kwon,Soon Hee Lee,Yong Dong Cho ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.1

The formation and chlorophyll composition of chlorophyll-protein complexes were investigated in the etiolated ginseng leaves during greening under continuous white light(2000 lux, 25℃, 66% relative humidity). Six chlorophyll-containing bands (CP1a, CP1, LHCP1, LHCP2, LHCP3, free pigment zone) were separated by SDSPAGE from ginseng leaves(grown under light). Chlorophyll a-rich CP1 complex and chlorophyll a/b-rich LHCP complexes reveal their maximum absorption peaks at 676 ㎚ and 670 ㎚, respectively. And chlorophyll a/b-rich LHCP complexes have another minor peak at 652nm. CPI and LHCP complexes have already formed in the etiolated ginseng leaves after 12 hr light treatment. And chlorophyll a/b ratio of CP1 complex and the percentages of chlorophyll contents of each complexes increased progressively during greening. These results suggest that the light-induced biosynthesis of CP complex molecules and the formation of functional PSI and PS II could occur within 12 hr of illumination in the etiolated ginseng leaves during greening.

터널 장공발파(2.0m 이상)에서 장약 및 기폭방법 개선과 전색효과를 증대시킨 터널고속굴착공법

강기돈(Kang Gi Don),박종범(Park Jong Bum),송치용(Song Chi Yong),조영동(Cho Young Dong),원연호(Won Yeon Ho) 대한토목학회 2010 대한토목학회 학술대회 Vol.2010 No.10