http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
다양한 유리화 용액과 동결기구에서 유리 동결 생쥐 포배기 배아의 생존율 비교
조동휴,이기숙,류철희,권중기,이정헌 한국임상수의학회 2012 한국임상수의학회지 Vol.29 No.1
The purpose of this study was to evaluate survival rates of vitrified mouse blastocysts in various vitrification solutions (cryoprotectants) and apparatuses. The mouse blastocysts were harvested from culture of mouse 2 cell embryo and were divided into three group (i) untreated (control); (ii) exposed to cryoprotectant agents; or (iii) cryopreserved by various vitrification apparatuses. Vitrification solutions are 40% ethylene glycol (EG) + 5.8 mg/mL ficoll + 0.5M sucrose (EFS solution), 3M glycerol + 3M EG (ES solution), 20% EG + 20% dimethyl sulfoxide (ED solution), 3M EG + 1.0 m sucrose (ES solution). Vitrification apparatuses consisted of 5 groups ; closed plastic straw (CPS), electron microscope (EM) grid, cryoloop, open pulled straw (OPS), and glass micropippete in plastic straw (GPS). The survival rates of control were 88.0%. The survival rates of exposed blastocysts in EFS, GE, ED, and ES solutions were 70.8%,43.5% (P<0.01), 83.3% and 65.2%, respectively. The survival rates of vitrified blastocysts in CPS, EM grid, cryoloop,OPS and GPS were 56.5% (P< 0.01), 72.7%, 83.3%, 60.9% (P<0.05) and 54.2% (P<0.01), respectively. Among the vitrification solutions, the highest survival rate was seen in blastocysts vitrified in EG + DMSO (83.3%). The survival rate was not significantly different from that of the control (88%). Blastocysts cryopreserved with glycerol in all groups had an overall low survival rate of 43.5%. Survival rate of mouse blastocysts between vitrification apparatuses showed higher in cryoloop.
조동휴,BHATTARAIJANARDHAN PRASAD,한성규 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1
Korean red ginseng (KRG) has been used worldwide as a traditional medicine for the treatment of various reproductive diseases. Gonadotropin releasing hormone (GnRH) neurons are the fundamental regulators of pulsatile release of gonadotropin required for fertility. In this study, an extract of KRG (KRGE) was applied to GnRH neurons to identify the receptors activated by KRGE. The brain slice patch clamp technique in whole cell and perforated patch was used to clarify the effect of KRGE on the membrane currents and membrane potentials of GnRH neurons. Application of KRGE (3 μg/μL) under whole cell patch induced remarkable inward currents (56.17±7.45 pA, n=25) and depolarization (12.91±3.80 mV, n=4) in GnRH neurons under high Cl- pipette solution condition. These inward currents were not only reproducible, but also concentration dependent. In addition, inward currents and depolarization induced by KRGE persisted in the presence of the voltage gated Na+ channel blocker tetrodotoxin (TTX),suggesting that the responses by KRGE were postsynaptic events. Application of KRGE under the gramicidin perforated patch induced depolarization in the presence of TTX suggesting its physiological signifi cance on GnRH response. Further, the KRGEinduced inward currents were partially blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; non-NMDA glutamate receptor antagonist, 10 μM) or picrotoxin (PIC; GABAA receptor antagonist, 50 μM), and almost blocked by PIC and CNQX mixture. Taken together, these results suggest that KRGE contains ingredients with possible GABA and non-NMDA glutamate receptor mimetic activity, and may play an important role in the endocrine function of reproductive physiology, via activation of GABAA and non-NMDA glutamate receptors in GnRH neurons.
보건직 여성공무원의 인유두종바이러스 감염에 대한 인식 및 지식조사
조동휴 ( Dong Hyu Cho ),김은경 ( Eun Kyung Kim ),이동현 ( Dong Hyun Lee ),최원구 ( Won Ku Choi ),임은지 ( Eun Ji Lim ),김종현 ( Jong Hyun Kim ),이정헌 ( Jeong Heon Lee ),조성남 ( Seong Nam Cho ),오병찬 ( Byung Chan Oh ),정영주 ( Y 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.5
목적: 보건직 여성공무원의 인유두종바이러스 감염에 대한 인식도와 지식도를 알아보기 위해 본 연구를 실시하였다. 연구 방법: 2007년 4월부터 6월까지 전라북도 보건직에 종사하는 공무원 여성 총 991명에게 인터넷을 통한 설문을 실시하였다. 사용된 설문은 일차적으로 대상자의 일반적 특성을 묻는 11문항, human papillomavirus (HPV) 감염에 대한 지식을 묻는 9문항으로 구성되었다. 결과: 완전하게 설문답변을 한 546명 중 인유두종바이러스에 대해 들어 본 적이 있다고 답한 여성은 145명 (37.6%)이었다. 인유두종바이러스 감염과 관련된 인식도는 기혼여성, 성경험이 있었던 여성, 과거 자궁경부암 검사상 이상이 있었던 경우, 성병기왕력이 있었던 경우, 정기적으로 1년에 한 번씩 자궁경부암 검진을 받고 있는 경우에 유의하게 높았다. HPV 감염에 대해 들어 본 적이 있다고 답한 여성에 있어서, HPV 감염에 대한 지식을 묻는 문항 정답률의 총 평균은 56.3%였다. 70% 이상 높은 정답률을 보인 질문은 4문항이었다. `HPV는 자궁경부암을 일으키는 주된 원인이다` 124명 (88.6%), `HPV는 성교를 통해 감염되는 질환이다` 111명 (79.3%), `피임약을 통해 HPV 감염을 예방할 수 없다` 107명 (76.4%), `남자가 HPV를 옮길수 있다` 100명 (71.4%). 정답률이 50% 미만은 3문항이었다. `HPV는 크게 고위험군과 저위험군 2가지로 나뉘어져 있다` 62명(44.3%), `HPV 감염은 평생 지속된다` 41명 (29.3%), `콘돔으로 HPV 감염을 예방할 수 있다` 18명 (12.9%). 결론: 보건직 여성공무원의 HPV 감염에 대한 인식도와 지식도는 낮은 편이었다. 따라서 여성들의 HPV 감염에 대한 인식 및지식 정도를 높이기 위해서는 매스미디어를 통한 더 많은 교육과 홍보가 필요하다. Objective: To assess the level and accuracy of understanding of human papillomavirus (HPV) infection among female public health personnel in Chonbuk province. Methods: Nine hundred ninety-one female public health personnel were asked to complete a questionnaire assessing HPV awareness and specific knowledge about the virus. The questionnaire contained 11 demographic informations and nine true-false questions on knowledge about HPV infection. Results: Questionnaires were completed by 546 women, of whom 145 (37.6%) had heard of HPV. Married women (P=0.019), those with a history of candida, genital warts (P<0.001), or an abnormal smear result (P=0.001), annually visitor for a Pap smear (P=0.023) were more likely to have heard of human papilloma virus. Medical doctor (38.6%) was the most common source of hearing of HPV. Overall percentage of knowledge among those who had heard of HPV was 56.3%. Responses indicated than more than 70% had up-to-date knowledge about several issues: HPV is the main cause of cervical cancer, HPV is sexually transmitted, The pill protects against HPV, and Men can carry HPV. Fewer than 50% knowledge of HPV infection were as follows: HPV viruses are divided to low-risk and high-risk type, HPV infections persist forever, condoms protect against HPV. Conclusion: In this well educated samples, awareness of HPV infection was poor. And also, knowledge about HPV infection was relatively low. We recommend that more educational effort is needed for improving women`s knowledge and awareness of HPV infection.
조동휴 ( Jo Dong Hyu ),정명자 ( Jeong Myeong Ja ),정영주 ( Jeong Yeong Ju ),진소자 ( Jin So Ja ),조성남 ( Jo Seong Nam ),김종덕 ( Kim Jong Deog ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.4
Cystic masses are among the most common sonographically detectable abnormalities of the umbilical cord. Umbilical cord cysts have been described in association with fetal anomalies, with chromosomal or structural defects being found in over 20% of cases.