소양호에서 분리한 Dnase 생성세균의 수리학적 분류

남인영,조기성 ( In Young Nam,Ki Seong Joh ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.1

To understand the structure of DNase producing bacterial community in Lake Soyang, water samples were analyzed in April, May and August, 1993. One hundred thirty strains of DNase producing bacteria were isolated by using DNase test agar plating method and classified by testing 40 characteristics. By using key dignostics, the dominant is Neisseria-Moraxella group in spring, Pseudomonas and Acinetobacter in summer, which were referenced by Bergey`s manual. The distribution of the bacteria in spring could be divided into 35 clusters and the bacteria in summer could be divided into 14 cluster in 90% similarity level. The population size of DNase producing bacteria was larger in summer than in spring. The diversity of the bacterial distribution and the number of major clusers in summer was decreased, because the similarity between the tested bacteria in summer is high.

Alcaligenes faecalis NS13에 의한 호기성 종속영양 질산화 및 탈질화

정택경,라창식,조기성,송홍규,Jung, Taeck-Kyung,Ra, Chang-Six,Joh, Ki-Seong,Song, Hong-Gyu 한국미생물학회 2016 미생물학회지 Vol.52 No.2

호기적 조건에서 질산화와 탈질화를 동시에 진행하는 Alcaligenes faecalis NS13 균주를 분리하여 다양한 특성을 파악하였다. 이 균주는 $15-37^{\circ}C$ 온도에서 생장할 수 있으며 암모니움 산화율이 높고 고농도의 암모니움 환경에서도 생장이 저해되지 않고 초기 암모니움 농도 증가에 따라 제거량이 증가하였다. pH와 염분농도에 대해서도 내성 범위가 넓어 암모니움 산화가 영향을 받지 않았다. 질산화에 이어진 탈질화로 인해 질산염의 축적이 일어나지 않았으며 탈질화의 중간산물인 아산화질소는 미량 검출되었지만 배양 후 모든 질소 화합물을 측정한 결과 약 42.8%가 $N_2$로 전환된 것으로 추정되었다. 탈질화는 PCR 증폭을 통해서 탈질화에 관여하는 유전자 nitrate reductase gene, napA과 nitrous oxide reductase gene, nosZ의 존재로 뒷받침되었다. 또한 배지 내 질소의 46.4%가 NS13 균주로 동화되었기 때문에 폐수처리 시 질산화 및 탈질화 후에 슬러지로 처분한다면 실질적으로 89% 이상의 우수한 암모니움의 제거효과를 거둘 수 있을 것이다. In order to find an efficient bacterial strain that can carry out nitrification and denitrification simultaneously, we isolated many heterotrophic nitrifying bacteria from wastewater treatment plant. One of isolates NS13 showed high removal rate of ammonium and was identified as Alcaligenes faecalis by analysis of its 16S rDNA sequence, carbon source utilization and fatty acids composition. This bacterium could remove over 99% of ammonium in a heterotrophic medium containing 140 mg/L of ammonium at pH 6-9, $25-37^{\circ}C$ and 0-4% of salt concentrations within 2 days. It showed even higher ammonium removal at higher initial ammonium concentration in the medium. A. faecalis NS13 could also reduce nitrate and nitrous oxide by nitrate reductase and nitrous oxide reductase, respectively, which was confirmed by detection of nitrate reductase gene, napA, and nitrous oxide reducase gene, nosZ, by PCR. One of metabolic intermediate of denitrification, $N_2O$ was detected from headspace of bacterial culture. Based on analysis of all nitrogen compounds in the bacterial culture, 42.8% of initial nitrogen seemed to be lost as nitrogen gas, and 46.4% of nitrogen was assimilated into bacterial biomass which can be removed as sludge in treatment processes. This bacterium was speculated to perform heterotrophic nitrification and aerobic denitrification simultaneously, and may be utilized for N removal in wastewater treatment processes.

중합효소연결반응에 의한 자연 수계에서의 mer 유전자 함유 세균 탐색

김상준,진주영,이현환,조기성 ( Sang Jun Kim,Joo Young Jin,Hyun Hwan Lee,Ki Seong Joh ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.1

The distribution of bacteria resistant to mercury (Hg^r-bacteria) was investigated from the coastal water sample of Kwangyang bay and freshwater samples of Taeback area, mer gene was also detected from the isolated Hg^r-bacteria by PCR and colony hybridization using the probes which correspond to the conserved sequence of the reported Tn 21, Tn 501 and pMJ 100. The distribution of Hg^r-bacteria at Kwangyang bay among the total number of heterotrophic bacteria grown on agar media was 0 - 0.15% and 0.05 - 0.084% in Feb. and Apr. respectively. The distribution of those bacteria at Taeback area was 94. 55% in the upstream and 17.78% in the downstream of the valley. From the Hg^r-bacteria, about 1Kb of mer gene was amplified by PCR using the amplified mer gene as probe, the interrelationship between the Hg^r-bacteria and the existence of mer gene was investigated by colony hybridization. As a result, 82% and 70% of the Hg^r-bacteria have mer gene at Kwangyang and Taeback area, respectively.

소양호에 존재하는 새로운 Flavobacterium의 분포와 특징

김하늘 ( Ha Neul Kim ),정요찬 ( Yo Chan Joung ),강희영 ( Hee Young Kang ),이범일 ( Beom Il Lee ),장태용 ( Tae Yong Jang ),조기성 ( Ki Seong Joh ) 한국환경생물학회 2012 환경생물 : 환경생물학회지 Vol.30 No.3

In this study, samples were collected from Lake Soyang in Kangwon-do in order to isolate novel Flavobacterium sp. strains. Totally, 21 strains of Flavobacterium showing 97%~98% similarity in 16S rRNA were selected and thoroughly investigated individual characteristics and ecological differences. As results, we could categorize Flavobacterium isolated from Lake Soyang into four major node groups, where most of Flavobacterium belonged to single group. Next, fatty acid analyses were performed demonstrating similar pattern of the majority of fatty acids as either iso C15:0 or summed feature 3 (comprised C16:1 ω7c and/or C16:1 ω6c) of the other Flavobacterium. However, other phenotypic data were different from the other Flavobacterium sp. Group. Our data showed that genetically related species of Flavobacterium have been distributed in Lake Soyang. Those Flavobacterium strains were phenotypically different from previously reported genus of Flavobacterium species. Taken together, we speculated that isolated Flavobacterium strains from Lake Soyang might be ecologically important members to maintain ecosystem.

馬山灣에서의 燐과 窒素의 動態에 관하여

안태석,안태영,권오섭,조기성 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

1978년 부터 1982년 까지 마산만 4개 정점에서 인산에 인과 질소 상태에 대한 조사 결과, 인산태 인과 질소 상태에 대한 조사 결과, 인산태 인은 각 정점별 평균치가 0.86∼2.70 μg at - P/ℓ이였고, Ammonia + amino 산태 질소는 1.21 ∼4.36 μg at - L/ℓ이였고, 질산태 질소는 0.94 ∼ μg at - N/ℓ 로 비교적 높은 값을 보였다. 마산시에 인접한 정점 1, 2에서는 질소의 농도가 점차로 증가하고 있는 경향을 보였다. 또한 질소와 인의 비율은 정점 1과 2에서 40을 넘는 값을 보여주어, 질소의 유입이 큰 것을 뜻하므로 이에대한 방지대책이 시급히 요구된다. The concentrations of phosphate-P, ammonia + amino acid-N, nitrite-N, and nitrate-N were measured at 4 sites in the polluted Masan Bay from 1978 to 1982. The mean concentration of phosphate-P, ammonia + amino acid-N, nitrine-N and nitrate-N in each site varied 0.68-1.90 ug at P/1, 1.21-4.36 ug at-N/1, 0.36-3.23 ug at-N/1 and 2.17-29.45 ug at-N/1, respectively. At site 1 and 2, nearyby Masan City, the concentration of nitrogen was gradually increasing according to the increase of the population of the city and the N/P ratios at site 1 and 2 reached more than 40.

마산만에서의 인과 질소의 동태에 관하여

안태석,안태영,권오섭,조기성 한국물환경학회 1986 한국물환경학회지 Vol.2 No.1

1978년 부터 1982년 까지 마산만 4개 정점에서 인산태 인과 질소 상태에 대한 조사 결과, 인산태 인은 각 정점별 평균치가 0.68-2.70㎍ at-P/ℓ이였고, Ammonia + amino 산태 질소는 1.21∼4.36㎍ at-N/ℓ이었고, 질산태 질소는 0.94∼29.45㎍ at N/ℓ로 비교적 높은 값을 보였다. 마산시에 인접한 정점 1, 2에서는 질소의 농도가 점차로 증가하고 있는 경향을 보였다. 또한 질소와 인의 비율은 정점 1과 2에서 40을 넘는 값을 보여주어, 질소의 유입이 큰 것을 뜻하므로 이에 대한 방지대책이 시급히 요구된다. The concentrations of phosphate-P, ammonia + amino acid-N, nitrite-N, and nitrate-N were measured at 4 sites in the polluted Masan Bay from 1978 to 1982. The mean concentration of phosphate-P, ammonia + amino acid-N, nitrite-N and nitrate-N in each site varied 0.68-1.90 ㎍ at-P/ℓ, 1.21-4.36 ㎍ at-N/ℓ, 0.36-3.23 ㎍ at-N/ℓ and 2.17-29.45 ㎍ at-N/ℓ, respectively. At site 1 and 2, nearby Masan City, the concentration of nitrogen was gradually increasing according to the increase of the population of the city and the N/P ratios at site 1 and 2 reached more than 40.