프로스타글란딘 E₁ 투영와 외과적 지연 처치가 당뇨 흰쥐의 횡복직근피판의 생존에 미치는 영향

조성필,전영준,송윤상,한기택 大韓成形外科學會 2002 Archives of Plastic Surgery Vol.29 No.6

馬山灣에서의 燐과 窒素의 動態에 관하여

안태석,안태영,권오섭,조기성 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

1978년 부터 1982년 까지 마산만 4개 정점에서 인산에 인과 질소 상태에 대한 조사 결과, 인산태 인과 질소 상태에 대한 조사 결과, 인산태 인은 각 정점별 평균치가 0.86∼2.70 μg at - P/ℓ이였고, Ammonia + amino 산태 질소는 1.21 ∼4.36 μg at - L/ℓ이였고, 질산태 질소는 0.94 ∼ μg at - N/ℓ 로 비교적 높은 값을 보였다. 마산시에 인접한 정점 1, 2에서는 질소의 농도가 점차로 증가하고 있는 경향을 보였다. 또한 질소와 인의 비율은 정점 1과 2에서 40을 넘는 값을 보여주어, 질소의 유입이 큰 것을 뜻하므로 이에대한 방지대책이 시급히 요구된다. The concentrations of phosphate-P, ammonia + amino acid-N, nitrite-N, and nitrate-N were measured at 4 sites in the polluted Masan Bay from 1978 to 1982. The mean concentration of phosphate-P, ammonia + amino acid-N, nitrine-N and nitrate-N in each site varied 0.68-1.90 ug at P/1, 1.21-4.36 ug at-N/1, 0.36-3.23 ug at-N/1 and 2.17-29.45 ug at-N/1, respectively. At site 1 and 2, nearyby Masan City, the concentration of nitrogen was gradually increasing according to the increase of the population of the city and the N/P ratios at site 1 and 2 reached more than 40.

手拈散이 再灌流裝置下의 흰쥐 摘出 心臟에 미치는 影響

高成奎,高昌男,金城煥,金永錫,文祥官,裵亨燮,曺基湖,李京燮 慶熙大學校韓醫科大學韓醫學硏究所 1997 慶熙韓醫大論文集 Vol.20 No.2

Background : The stenosis of the coronary artery may result in a decrease in the myocardial oxygen supply, ischemia and ??. Soojeomsan as a drug of analgesics is generally regarded to have the effect of vitalizing blood and ?? blood stasis(活血祛瘀). Methods : The purpose of this experimental study is to md whether Soojeomsan is effective or not in reperfusing ischemia in ?? heart of rats under the Langendorff apparatus. Ischemia was induced in isolated hearts of Sprague-Dawley ?? by ceasing the perfusion for 20 minutes. The experiments were divided into a normal saline orally administered group(control group), a Soojeomsan orally 120mg administered group(sample A) and a Soojeomsan orally 240mg administered group(sample B). The heart rates, developed left ventricular pressure, myocardial dilatation/contraction, cardiac blood flow and cardiac enzyme(Lactic Dehydrogenase) of the three group were measured and compared in order to assess the influence of Soojeomsan on isolated rat heart's recovering ability from ischemia. Results : 1. Heart rates were significantly increased in Soojeomsan orally 120mg administered group in comparison with control group on perfusion (P<0.05), and Soojeomsan orally 120mg administered group and Soojeomsan orally 240mg administered group in comparison with control group on ischemia-reperfusion(P<0.001). 2. Left ventricular pressure were significantly increased in Soojeomsan orally 120mg administered group and 240mg administered group in comparison with control group on perfusion(P<0.01), But there were no significant effects on ischemia-reperfusion. 3. There were no significant effects of the ability of left ventricle contraction on perfusion, But the ability of left ventricle contraction was significantly promoted in Soojeomsan orally 120mg administered group in comparison with control group on ischemia-reperfusion(P<0.05). 4. There were no significant effects of the ability of left ventricle dilatation on perfusion, But the ability of left ventricle dilatation was significantly promoted in Soojeomsan orally 120mg administered group in comparison with control group on ischemia-reperfusion(P<0.05). 5. No significant effects were observed in the Coronary perfusion flow on perfusion and ischemia-reperfusion in comparison with control group. 6. There were no significant effects of reducing of Lactic Dehydrogenase on perfusion, But Lactic Dehydrogenase was significantly reduced in Soojeomsan orally 120mg administered group in comparison with control group on ischemia-reperfusion(P<0.01). Conclusion : Soojeomsan have effects to protect of myocardial work and to recover ischemia in the isolated rat hearts. Especially. the effect of Soojeomsan orally 120mg administered group is greater than of Soojeomsan orally 240mg administered group and control group on perfusion and ischemia-reperfusion.

간암종에서 9p, 13q, 16q 염색체의 Microsatellite 변이에 대한 연구

조성진(Seong Jin Cho),김남렬(Nam Ryeol Kim),민연기(Youn Ki Min),조용걸(Yong Geul Joh),조민영(Min Young Cho),서성욱(Sung Ock Suh),염범우(Bom Woo Yeom),원남희(Nam Hee Won) 대한외과학회 2001 Annals of Surgical Treatment and Research Vol.61 No.3

川黃連이 自發性 高血壓 흰쥐(SHR)의 血壓에 미치는 影響

高成奎,高昌男,韓昌澔,金永錫,文祥官,李京燮,曺基湖,裵亨燮 慶熙大學校韓醫科大學韓醫學硏究所 1997 慶熙韓醫大論文集 Vol.20 No.2

In order to investigate the effects of Coptis chenensis FRANCH(川黃連) extract solution on the blood pressure, experimental studies were performed on the blood pressure in normal rats, SHRs. The results were as follows. 1. Coptis chenensis FRANCH(川黃連) extract solution showed significantly effect of asing odecren the mean blood pressure in normal rats. 2. Coptis chenensis FRANCH(川黃連) extract solution showed significantly effect of decreasing on the systolic blood pressure in SHRs. 3. Coptis chenensis FRANCH(川黃連) extract solution was dilated on the isolated aorta in rabbits. 4. Coptis chenensis FRANCH(川黃連) extract solution showed significantly inhibitory effect of ACE in vitro. 5. The mechanism of the antihypertensive effect on Coptis chenensis FRANCH(川黃連) seems due to vasodilatation. According to the above results, it was recognized that Coptis chenensis FRANCH(川黃連) showed significantly antihypertensive effect in normal rats and SHRs. And the mechanism of the antihypertensive effect on Coptis chenensis FRANCH(川黃連) seems due to vasodilatation and ACE inhibition(in vitro).

A report of 21 unrecorded bacterial species of Korea belonging to the phylum Bacteroidota isolated in 2021

차창준,전체옥,Ki-seong Joh,Won Yong Kim,김승범,김명겸,Jung-Hoon Yoon 국립생물자원관 2023 Journal of species research Vol.12 No.-

During screening for indigenous prokaryotic species in Republic of Korea in 2021, a total of 21 bacterial strains assigned to the phylum Bacteroidota were isolated from a variety of environmental habitats including pine cone, seaweed, soil, sea sediment, brackish water and moss. Based on the 16S rRNA gene sequence similarity value of more than 98.7% and formation of a robust phylogenetic clade with the type strain of the closest bacterial species, it was found that the 21 strains belong to independent and recognized bacterial species. There has been no official report that the identified 21 species have been isolated in Republic of Korea up to date. Therefore, 16 species in six genera of two families in the order Flavobacteriales, two species in two genera of two families in the order Cytophagales, one species in one genus of one family in the order Chitinophagales and two species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidota isolated in Republic of Korea. Their Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

유리피판술을 이용한 구축성 화상반흔의 교정

임 풍,허 진,조성필,장익수,한기택 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.3

Burn scar contractures of the various joint areas including anterior cervical region, popliteal region, ankle, antecubital region and wrist accompanied with severe functional limitations and esthetic problems. Many authors recommended full-thickness, split-thickness skin graft, local skin flap, or distant skin flap. But the recurrence of the contracture and many following procedures have been troublesome after these surgeries. Recent progress in the field of microvascular surgery has opened the way for various types of free transfer. The microvascular surgery enabled the various joint regions to be resurfaced with free flap and so the recurrence of contracture was prevented. Authors treated 20 patients with severe and recurrent burn scar contracture of the various joint regions using various microvascular free flap transfer. We obtained satisfactory functional results without late recurrence of the contracture of the transferred flaps.

Report of 21 unrecorded bacterial species in Korea belonging to Betaproteobacteria and Epsilonproteobacteria

Kim, Min-Kyeong,Seong, Chi-Nam,Jahng, Kwangyeop,Cha, Chang-Jun,Joh, Ki-seong,Bae, Jin-Woo,Cho, Jang-Cheon,Im, Wan-Taek,Kim, Seung-Bum The National Institute of Biological Resources 2017 Journal of species research Vol.6 No.1

During the extensive survey of the prokaryotic species diversity in Korea, bacterial strains belonging to Betaproteobacteria and Epsilonproteobacteria were isolated from various sources including freshwater, sediment, soil and fish. A total of 23 isolates were obtained, among which 22 strains were assigned to the class Betaproteobacteria and one strain to the class Epsilonproteobacteria. The 22 betaproteobacterial strains were further assigned to Comamonadaceae (11 strains), Burkholderiaceae (6 strains), Oxalobacteraceae (2 strains), Neisseriaceae (1 strain) and unclassified family groups (2 strains). For the strains of Burkholderiaceae, 3 strains were identified as 3 species of Burkholderia, and 2 strains were as 2 species of Cupriavidus. For the strains of Comamonadaceae, 4 strains were identified as 2 species of the genus Hydrogenophaga, 2 strains as 2 species of Acidovorax, 2 strains as 2 species of Limnohabitans, and each of the remaining strains as single species of Comamonas, Curvibacter and Rhodoferax, respectively. For the strains of Oxalobacteraceae, 1 strain was identified as a species of Undibacterium, and the other strain as a species of Herbaspirillum. The strain belonging to Neisseriaceae was identified as a species of Iodobacter. The remaining strains of Betaproteobacteria were identified as species of Sphaerotilus and Methylibium respectively (family unassigned). The epsilonproteobacterial strain was identified as a species of Arcobacter of the family Camplyobacteraceae. The detailed description of each unrecorded species is provided.

Rapid Detection of Virulence Factors of Aeromonas Isolated from a Trout Farm by Hexaplex-PCR

Nam, In-Young,Joh, Ki-Seong The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.4

The detection of virulence factors of Aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. In this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and Aeromonas species were isolated and identified. For rapid detection of six virulence factors of isolated Aeromonas, a hexaplex-polymerase chain reaction (hexaplex-PCR) assay was used. The detected virulence factors include aerolysin (aer), GCAT (gcat), serine protease (ser), nuclease (nuc) lipase (lip) and lateral flagella (laf). The dominant strain found in our isolates was Aeromonas sobria, and the dominant virulence factors were aer and nuc for all seasons. We confirmed that A. sobria and two of the virulence genes (aer and nuc) are related. We proposed a method by which one can identify the major strains of Aeromonas: A. hydrophila, A. sobria, A. caviae, and A. veronii, using hexaplex-PCR.

A Novel Marker for the Species-Specific Detection and Quantitation of Vibrio cholerae by Targeting an Outer Membrane Lipoprotein lolB Gene

( Min Seok Cho ),( Tae Young Ahn ),( Ki Seong Joh ),( Soon Young Paik ),( Oh Sang Kwon ),( Won Hwa Jheong ),( Yochan Joung ),( Dong Suk Park ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.