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중합효소연결반응에 의한 자연 수계에서의 mer 유전자 함유 세균 탐색
김상준,진주영,이현환,조기성 ( Sang Jun Kim,Joo Young Jin,Hyun Hwan Lee,Ki Seong Joh ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.1
The distribution of bacteria resistant to mercury (Hg^r-bacteria) was investigated from the coastal water sample of Kwangyang bay and freshwater samples of Taeback area, mer gene was also detected from the isolated Hg^r-bacteria by PCR and colony hybridization using the probes which correspond to the conserved sequence of the reported Tn 21, Tn 501 and pMJ 100. The distribution of Hg^r-bacteria at Kwangyang bay among the total number of heterotrophic bacteria grown on agar media was 0 - 0.15% and 0.05 - 0.084% in Feb. and Apr. respectively. The distribution of those bacteria at Taeback area was 94. 55% in the upstream and 17.78% in the downstream of the valley. From the Hg^r-bacteria, about 1Kb of mer gene was amplified by PCR using the amplified mer gene as probe, the interrelationship between the Hg^r-bacteria and the existence of mer gene was investigated by colony hybridization. As a result, 82% and 70% of the Hg^r-bacteria have mer gene at Kwangyang and Taeback area, respectively.